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The map label for this gene is mdh

Identifier: 15835270

GI number: 15835270

Start: 783057

End: 784037

Strand: Reverse

Name: mdh

Synonym: TC0655

Alternate gene names: 15835270

Gene position: 784037-783057 (Counterclockwise)

Preceding gene: 15835271

Following gene: 15835259

Centisome position: 73.07

GC content: 44.95

Gene sequence:

>981_bases
ATGTTTTCTCAAACAGTGAGTGTAGCAGTAACAGGAGGAACGGGGCAAATCGCCTACTGCTTTTTATTTGCCTTGGCCCA
TGGGGATATTTTTGGGCCTGATACCGGAATAGATCTGCGTATTTATGATATCCCAGGGACAGAAAGATCTCTATCTGGAG
TACGCATGGAATTGGATGATGGAGCTTTCCCTTTGTTGCAACGCGTGCAGGTAACGACATCCTTGCATGATGCTTTTGAT
GACATCGATGCTGCCTTTCTCATAGGATCTGTCCCCAGAGGCCCAGGAATGGAAAGAAGAGATCTTTTGAAGAAAAATGG
CGAGATTTTTGCTACTCAAGGTAAGGTCTTGAACACAGCAGCTAAACGAGAGGCCAAAATTTTTGTCGTTGGAAATCCTG
TGAATACAAATTGCTGGATAGCCATGAATCACGCTCCTAGATTGCTGAGAAAAAATTTTCATGCAATGTTGCGCTTAGAT
CAGAATCGTATGCACAGTATGCTAGCCCATAGGGCTGAAGTCCCTTTATCTGCTGTATCGCAGGTTGTTGTCTGGGGGAA
TCATTCTGCAAAACAAGTTCCAGATTTTACACAGGCATTAATTCATGGTCGTCCTATTGTAGAAACTATAGCGGACCGCG
ATTGGCTAGAGAATATTATGGTTCCTTCTGTGCAGAGTCGTGGTAGCGCTGTAATAGAAGCGCGAGGGAAGTCTTCTGCA
GCTTCTGCAGCACGAGCTTTAGCAGAGGCTGCACGATCCATATATCAACCGAAGGAAGGGGAATGGTTTTCTTCTGGAGT
ATGTTCGGACAATAATCCCTATGGCTTGCCAGAAGATATAATCTTTGGGTTCCCTTGTCGAATGCTGGGGACTGGAGAGT
ATGAGATCGTCCCCGGACTTCCTTGGGATGCCTTTATTCGTGGAAAGATGCAGATTTCTTTAGACGAAATTCTGCAAGAG
AAAGCTAGTGTCTCTTTGTAA

Upstream 100 bases:

>100_bases
CTTGATTCTTTTATTCTCCAAACGTATGTTGGGTTCAAAATTGATTACAAGCGTATCATGAAGAATATATGCGTTTTGTA
AAAGCAAGAGAGGGTGGTTT

Downstream 100 bases:

>100_bases
GGCATTAATCCATCCTCCCTCTCTAAAGGGAGGATGGATTAAGAAGCTTCCGGAGCATAAAGAAATTCTTTAGCTATGTA
TGCCGTAGAATCTCGCAGTA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MFSQTVSVAVTGGTGQIAYCFLFALAHGDIFGPDTGIDLRIYDIPGTERSLSGVRMELDDGAFPLLQRVQVTTSLHDAFD
DIDAAFLIGSVPRGPGMERRDLLKKNGEIFATQGKVLNTAAKREAKIFVVGNPVNTNCWIAMNHAPRLLRKNFHAMLRLD
QNRMHSMLAHRAEVPLSAVSQVVVWGNHSAKQVPDFTQALIHGRPIVETIADRDWLENIMVPSVQSRGSAVIEARGKSSA
ASAARALAEAARSIYQPKEGEWFSSGVCSDNNPYGLPEDIIFGFPCRMLGTGEYEIVPGLPWDAFIRGKMQISLDEILQE
KASVSL

Sequences:

>Translated_326_residues
MFSQTVSVAVTGGTGQIAYCFLFALAHGDIFGPDTGIDLRIYDIPGTERSLSGVRMELDDGAFPLLQRVQVTTSLHDAFD
DIDAAFLIGSVPRGPGMERRDLLKKNGEIFATQGKVLNTAAKREAKIFVVGNPVNTNCWIAMNHAPRLLRKNFHAMLRLD
QNRMHSMLAHRAEVPLSAVSQVVVWGNHSAKQVPDFTQALIHGRPIVETIADRDWLENIMVPSVQSRGSAVIEARGKSSA
ASAARALAEAARSIYQPKEGEWFSSGVCSDNNPYGLPEDIIFGFPCRMLGTGEYEIVPGLPWDAFIRGKMQISLDEILQE
KASVSL
>Mature_326_residues
MFSQTVSVAVTGGTGQIAYCFLFALAHGDIFGPDTGIDLRIYDIPGTERSLSGVRMELDDGAFPLLQRVQVTTSLHDAFD
DIDAAFLIGSVPRGPGMERRDLLKKNGEIFATQGKVLNTAAKREAKIFVVGNPVNTNCWIAMNHAPRLLRKNFHAMLRLD
QNRMHSMLAHRAEVPLSAVSQVVVWGNHSAKQVPDFTQALIHGRPIVETIADRDWLENIMVPSVQSRGSAVIEARGKSSA
ASAARALAEAARSIYQPKEGEWFSSGVCSDNNPYGLPEDIIFGFPCRMLGTGEYEIVPGLPWDAFIRGKMQISLDEILQE
KASVSL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=327, Percent_Identity=43.4250764525994, Blast_Score=269, Evalue=2e-72,
Organism=Homo sapiens, GI89886456, Length=297, Percent_Identity=22.2222222222222, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17561064, Length=326, Percent_Identity=40.7975460122699, Blast_Score=218, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI32566798, Length=240, Percent_Identity=41.6666666666667, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI32566800, Length=172, Percent_Identity=37.2093023255814, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583394, Length=325, Percent_Identity=43.3846153846154, Blast_Score=248, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MDH_CHLMU (Q9PK18)

Other databases:

- EMBL:   AE002160
- PIR:   C81678
- RefSeq:   NP_297029.1
- ProteinModelPortal:   Q9PK18
- SMR:   Q9PK18
- GeneID:   1246016
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0655
- TIGR:   TC_0655
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- BioCyc:   CMUR243161:TC_0655-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 35679; Mature: 35679

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 188-188 BINDING 93-93 BINDING 99-99 BINDING 106-106 BINDING 113-113 BINDING 132-132 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSQTVSVAVTGGTGQIAYCFLFALAHGDIFGPDTGIDLRIYDIPGTERSLSGVRMELDD
CCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECC
GAFPLLQRVQVTTSLHDAFDDIDAAFLIGSVPRGPGMERRDLLKKNGEIFATQGKVLNTA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCEEEECCCHHHHH
AKREAKIFVVGNPVNTNCWIAMNHAPRLLRKNFHAMLRLDQNRMHSMLAHRAEVPLSAVS
HCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHEECHHHHHHHHHHHHCCCHHHHC
QVVVWGNHSAKQVPDFTQALIHGRPIVETIADRDWLENIMVPSVQSRGSAVIEARGKSSA
EEEEECCCCCHHCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHH
ASAARALAEAARSIYQPKEGEWFSSGVCSDNNPYGLPEDIIFGFPCRMLGTGEYEIVPGL
HHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCHHHHCCCCHHEECCCCEEEECCC
PWDAFIRGKMQISLDEILQEKASVSL
CHHHHEECEEEECHHHHHHHHHCCCC
>Mature Secondary Structure
MFSQTVSVAVTGGTGQIAYCFLFALAHGDIFGPDTGIDLRIYDIPGTERSLSGVRMELDD
CCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECC
GAFPLLQRVQVTTSLHDAFDDIDAAFLIGSVPRGPGMERRDLLKKNGEIFATQGKVLNTA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCEEEECCCHHHHH
AKREAKIFVVGNPVNTNCWIAMNHAPRLLRKNFHAMLRLDQNRMHSMLAHRAEVPLSAVS
HCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHEECHHHHHHHHHHHHCCCHHHHC
QVVVWGNHSAKQVPDFTQALIHGRPIVETIADRDWLENIMVPSVQSRGSAVIEARGKSSA
EEEEECCCCCHHCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHH
ASAARALAEAARSIYQPKEGEWFSSGVCSDNNPYGLPEDIIFGFPCRMLGTGEYEIVPGL
HHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCHHHHCCCCHHEECCCCEEEECCC
PWDAFIRGKMQISLDEILQEKASVSL
CHHHHEECEEEECHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935