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The map label for this gene is ksgA

Identifier: 15835248

GI number: 15835248

Start: 758691

End: 759524

Strand: Reverse

Name: ksgA

Synonym: TC0633

Alternate gene names: 15835248

Gene position: 759524-758691 (Counterclockwise)

Preceding gene: 15835249

Following gene: 15835247

Centisome position: 70.79

GC content: 41.49

Gene sequence:

>834_bases
GTGGCACGGAGTTCTATAGAGCAGTTAACTTCTTTTCTCAAATCGGTCAACGGGCGGGCCAAGAAGGCTCTTTCCCAAAA
TTTTTTAGTGGACGGGAACATTTTACGAAAAATTCTGGCAACTGCGGATGTTCAGCCTGGGGATTGGGTTCTTGAAATAG
GTCCTGGATTTGGAGCGTTATCCGAAGTTCTGGTCTCTCAAGGAGCAAATGTCATTGCTCTGGAAAAAGATCCCATGTTT
GAAGAGTCTTTATCTCAACTGCCTATCGATATTGAGATTACGGATGCTTGTAAGTATCCTCTAGCCTCTTTAGACGATAA
GGGGTGGAAAGGGAAAGGACGTATAGTAGCGAACCTTCCATACCATATTACCACTCCTTTATTAACAAAGTTCTTTTTAG
AATGCCCTAATCGTTGGAAGACGGTCACTGTAATGATTCAAGATGAAGTGGCTCGTCGGATTACCGCCAATCCTGGGGAC
AAGGATTACAGTTCGTTGACGGTTTTTTTGCGATTTTTCGCTGATGTGCAATATGCCTTTAAAGTGAGCCCAAACTGTTT
CTATCCTAAGCCGAGTGTGAGTTCGGCTGTCGTTCATATGCGTGTGCATGAGGATTTTCCTTTAAGTGGTTCTGAGATAG
ACGAGTTTTTTGCGTTAACTCGGGCAGCATTTGGACAAAGACGAAAACTACTAGCAAACTCTTTGAAAAATCTGTATCCA
AAAGATAAGGTCTTTCAAGTGTTGGAGCATCTAGGGTTTTCTGAGAAAACTAGACCAGAGACTATTTCTCTAGAGGAATA
TTTAAAAATTTTTCGTTTGTTAAAAGATTTTTAG

Upstream 100 bases:

>100_bases
AATTGCGCGCTCGCTATGGGTTCGATCCTTCTATCATAACACAACTTCTTTCAGAAGATGCTCCCCAGCTATTTTCTTTA
TTATAAAAAAGGCGGAAAGG

Downstream 100 bases:

>100_bases
TAGTTTTGCTCATCTCTACAGAAAATAAGGCTTCTCTTTCCAAATATCTTCCTTTCGATCACAATATTCGCTCTAGGTGT
CTTGGATGCTTATAGCATTC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MARSSIEQLTSFLKSVNGRAKKALSQNFLVDGNILRKILATADVQPGDWVLEIGPGFGALSEVLVSQGANVIALEKDPMF
EESLSQLPIDIEITDACKYPLASLDDKGWKGKGRIVANLPYHITTPLLTKFFLECPNRWKTVTVMIQDEVARRITANPGD
KDYSSLTVFLRFFADVQYAFKVSPNCFYPKPSVSSAVVHMRVHEDFPLSGSEIDEFFALTRAAFGQRRKLLANSLKNLYP
KDKVFQVLEHLGFSEKTRPETISLEEYLKIFRLLKDF

Sequences:

>Translated_277_residues
MARSSIEQLTSFLKSVNGRAKKALSQNFLVDGNILRKILATADVQPGDWVLEIGPGFGALSEVLVSQGANVIALEKDPMF
EESLSQLPIDIEITDACKYPLASLDDKGWKGKGRIVANLPYHITTPLLTKFFLECPNRWKTVTVMIQDEVARRITANPGD
KDYSSLTVFLRFFADVQYAFKVSPNCFYPKPSVSSAVVHMRVHEDFPLSGSEIDEFFALTRAAFGQRRKLLANSLKNLYP
KDKVFQVLEHLGFSEKTRPETISLEEYLKIFRLLKDF
>Mature_276_residues
ARSSIEQLTSFLKSVNGRAKKALSQNFLVDGNILRKILATADVQPGDWVLEIGPGFGALSEVLVSQGANVIALEKDPMFE
ESLSQLPIDIEITDACKYPLASLDDKGWKGKGRIVANLPYHITTPLLTKFFLECPNRWKTVTVMIQDEVARRITANPGDK
DYSSLTVFLRFFADVQYAFKVSPNCFYPKPSVSSAVVHMRVHEDFPLSGSEIDEFFALTRAAFGQRRKLLANSLKNLYPK
DKVFQVLEHLGFSEKTRPETISLEEYLKIFRLLKDF

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=299, Percent_Identity=29.4314381270903, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI156415992, Length=288, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1786236, Length=262, Percent_Identity=31.2977099236641, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25141369, Length=282, Percent_Identity=29.4326241134752, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25146882, Length=250, Percent_Identity=28.4, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=222, Percent_Identity=29.2792792792793, Blast_Score=99, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21358017, Length=223, Percent_Identity=32.2869955156951, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21357273, Length=289, Percent_Identity=27.681660899654, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_CHLMU (Q9PK40)

Other databases:

- EMBL:   AE002160
- PIR:   D81680
- RefSeq:   NP_297007.1
- ProteinModelPortal:   Q9PK40
- SMR:   Q9PK40
- GeneID:   1245993
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0633
- TIGR:   TC_0633
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- PhylomeDB:   Q9PK40
- ProtClustDB:   PRK00274
- BioCyc:   CMUR243161:TC_0633-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31228; Mature: 31097

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 27-27 BINDING 29-29 BINDING 54-54 BINDING 75-75 BINDING 95-95 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSSIEQLTSFLKSVNGRAKKALSQNFLVDGNILRKILATADVQPGDWVLEIGPGFGAL
CCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHH
SEVLVSQGANVIALEKDPMFEESLSQLPIDIEITDACKYPLASLDDKGWKGKGRIVANLP
HHHHHHCCCCEEEECCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECC
YHITTPLLTKFFLECPNRWKTVTVMIQDEVARRITANPGDKDYSSLTVFLRFFADVQYAF
CHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEE
KVSPNCFYPKPSVSSAVVHMRVHEDFPLSGSEIDEFFALTRAAFGQRRKLLANSLKNLYP
EECCCCCCCCCCCCHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KDKVFQVLEHLGFSEKTRPETISLEEYLKIFRLLKDF
HHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARSSIEQLTSFLKSVNGRAKKALSQNFLVDGNILRKILATADVQPGDWVLEIGPGFGAL
CHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHH
SEVLVSQGANVIALEKDPMFEESLSQLPIDIEITDACKYPLASLDDKGWKGKGRIVANLP
HHHHHHCCCCEEEECCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECC
YHITTPLLTKFFLECPNRWKTVTVMIQDEVARRITANPGDKDYSSLTVFLRFFADVQYAF
CHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEE
KVSPNCFYPKPSVSSAVVHMRVHEDFPLSGSEIDEFFALTRAAFGQRRKLLANSLKNLYP
EECCCCCCCCCCCCHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KDKVFQVLEHLGFSEKTRPETISLEEYLKIFRLLKDF
HHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935