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The map label for this gene is def

Identifier: 15835247

GI number: 15835247

Start: 757895

End: 758440

Strand: Reverse

Name: def

Synonym: TC0632

Alternate gene names: 15835247

Gene position: 758440-757895 (Counterclockwise)

Preceding gene: 15835248

Following gene: 15835246

Centisome position: 70.69

GC content: 39.56

Gene sequence:

>546_bases
ATGATTAGAGATCTTGAGTATTATGATAGCCCGATTTTGCGTAAAGTTGCGGCTCCTATAGATGAAATCACGGATGAGCT
ACGACAACTTGTACTAGATATGAGTGAGACTATGACTTTTTATAAAGGAGTGGGACTCGCAGCTCCTCAGGTAGGACATA
GTGTTGCTTTATTCATTATGGGCGTAGAAAAAGAGCTGGACGATGGAGAGCTCATTTTCTGTGATTTCCCAAAAGTATTC
ATTAATCCTGTGATTACTCAAAAATCTGAGCAGTTAGTTTATGGAAATGAAGGGTGTTTGTCTATTCCAGGATTGAGAGG
AGAAGTGGCTAGGCCCGATAAAATTACGGTAACAGCAAAAAATTTGGATGGCCAACCATTTTCTATGACTCTAGAGGGTT
TTTTGGCGAGAATCGTCATGCATGAAACCGATCACCTGCATGGAGTCCTCTATATCGATAGAATGTCTGATAAAGACAAG
ACGAAACAGTTTAAGAATAACCTAGAGAAAATTCGCCGAAAATATAGTATTTTGCGAGGCTTATAG

Upstream 100 bases:

>100_bases
GCTAAAGGATAATGTTGGAAGATGCTGTTTAATTGTTTTTAGATCATAGGTTTACAAACGGCCTATCTTTTTAAGTTTTT
GTGTGGGAAGCTTTGGGATT

Downstream 100 bases:

>100_bases
TTTTTGGTGTTTCTTGCTGTAAGCACCTTCTAAGCTGGCGACTTTAAAGTTTTTTTCTCTTTGCTTATACTTATGCCACG
CTACAGCATAAGAAGAGCGT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MIRDLEYYDSPILRKVAAPIDEITDELRQLVLDMSETMTFYKGVGLAAPQVGHSVALFIMGVEKELDDGELIFCDFPKVF
INPVITQKSEQLVYGNEGCLSIPGLRGEVARPDKITVTAKNLDGQPFSMTLEGFLARIVMHETDHLHGVLYIDRMSDKDK
TKQFKNNLEKIRRKYSILRGL

Sequences:

>Translated_181_residues
MIRDLEYYDSPILRKVAAPIDEITDELRQLVLDMSETMTFYKGVGLAAPQVGHSVALFIMGVEKELDDGELIFCDFPKVF
INPVITQKSEQLVYGNEGCLSIPGLRGEVARPDKITVTAKNLDGQPFSMTLEGFLARIVMHETDHLHGVLYIDRMSDKDK
TKQFKNNLEKIRRKYSILRGL
>Mature_181_residues
MIRDLEYYDSPILRKVAAPIDEITDELRQLVLDMSETMTFYKGVGLAAPQVGHSVALFIMGVEKELDDGELIFCDFPKVF
INPVITQKSEQLVYGNEGCLSIPGLRGEVARPDKITVTAKNLDGQPFSMTLEGFLARIVMHETDHLHGVLYIDRMSDKDK
TKQFKNNLEKIRRKYSILRGL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=169, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=5e-16,
Organism=Escherichia coli, GI1789682, Length=161, Percent_Identity=37.888198757764, Blast_Score=102, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24645728, Length=156, Percent_Identity=37.1794871794872, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_CHLMU (Q9PK41)

Other databases:

- EMBL:   AE002160
- PIR:   C81680
- RefSeq:   NP_297006.1
- ProteinModelPortal:   Q9PK41
- SMR:   Q9PK41
- GeneID:   1245992
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0632
- TIGR:   TC_0632
- HOGENOM:   HBG665227
- OMA:   VINPTWE
- ProtClustDB:   PRK00150
- BioCyc:   CMUR243161:TC_0632-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20535; Mature: 20535

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: ACT_SITE 142-142

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRDLEYYDSPILRKVAAPIDEITDELRQLVLDMSETMTFYKGVGLAAPQVGHSVALFIM
CCCCCCHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEE
GVEKELDDGELIFCDFPKVFINPVITQKSEQLVYGNEGCLSIPGLRGEVARPDKITVTAK
ECCCCCCCCCEEEEECCHHHHHHHHCCCCCEEEECCCCCEECCCCCCCCCCCCEEEEEEC
NLDGQPFSMTLEGFLARIVMHETDHLHGVLYIDRMSDKDKTKQFKNNLEKIRRKYSILRG
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
L
C
>Mature Secondary Structure
MIRDLEYYDSPILRKVAAPIDEITDELRQLVLDMSETMTFYKGVGLAAPQVGHSVALFIM
CCCCCCHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEE
GVEKELDDGELIFCDFPKVFINPVITQKSEQLVYGNEGCLSIPGLRGEVARPDKITVTAK
ECCCCCCCCCEEEEECCHHHHHHHHCCCCCEEEECCCCCEECCCCCCCCCCCCEEEEEEC
NLDGQPFSMTLEGFLARIVMHETDHLHGVLYIDRMSDKDKTKQFKNNLEKIRRKYSILRG
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935