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The map label for this gene is hemL

Identifier: 15835100

GI number: 15835100

Start: 586942

End: 588210

Strand: Direct

Name: hemL

Synonym: TC0482

Alternate gene names: 15835100

Gene position: 586942-588210 (Clockwise)

Preceding gene: 15835097

Following gene: 15835105

Centisome position: 54.7

GC content: 40.58

Gene sequence:

>1269_bases
ATGCCCCATCTTTTCTCTAAAGCTTGTCAATATTTCCCAGGAGGAGTCAACTCTCCTGTTCGAGCGTGCCGATCTGTTGA
TATAACCCCTCCCGTAGTAACAAGAGCTTCGGGAGATTTTTTTACGGATTCTCAAGGTAAAACATATATTGATTTTTGTG
GATCCTGGGGATCTTTGATCCACGGTCATAGTCATCCCTATATTTGCGAAGCAATCCAACAGGGCCTACAAAGGGGATGT
TCCTATGGTCTTACTTCTGAACAGGAAATTTCATTTGCAGAAGAAATCTTTTCTTACTTAGAGATCTCCAACGACCATAA
AATTCGATTCATGTCCACAGGATCAGAAGCTACTATGACCGCCGTTCGTCTTGCGAGAGGAGTCACAGAACGCCCTATTA
TCATTAAATTTTCAGGATGCTATCACGGTCATTCAGATGTTTTTTTACAAGAGATCCACTTCCAACAAACTATTCTAGAC
ACTGTTGATCTCACCCAACCACTTACTCTTTCCCTTCCTTTCAATAATCTATCTCTGTTCCTAGATGTTATGAATCAAAT
AGGTCATAGAGTCGCTGGAGTCATTTTTGAGCCTATATGTGCAAATATGGGAGTTGTTCTTCCCTTACCAGGTTTTATAG
AAGGCATTATTCAAACCTGTAGAAAAACAGGATCCCTTTCTATCATGGATGAAGTTGTTACAGGATTCCGAGTAAGCAAA
GGCGGAGCAGCAGCTTTACACCCGTTAAAACCGGATATCCTTGTTTTCGGAAAAATTTTAGGTGGAGGATTGCCAGCATC
GGCAGTTTGCGCTCCCGCAGCAATCATGGATTACCTAGCCCCTGTTGGGAAAGTTTTTCAAGCAGGCACTTTATCAGGAA
ACCCCTTAGCTATGTCTGCAGGGAAAGCTTCCATAGCCTTGTGTCGAGAAAAGAATTTTTATACTCAACTGTCTGCTATT
GAGGATGATTTTTTATCCCCTATCGAACAAATGATTCAACAATCAGGTATTCCCGTCACACTTGTTCGTTATGGAAATCT
TTTCTCCTTTTTCTTTAGCGAGAATCGACCTAATAACCTTAAAGAAGTTCAGCTGTGTAATACCGATATTTTTCGAACTT
TTTATCAACAGGCCTTCTTAAAAGGAATTTATCTTTCTCCATCACCTTTCGAAGCGAGTTTCTTGTCCACAGCTCACTCC
ATGGAAAATTTGAACTATGCTCAACAAGTTTTAATAGAAAGCTTAGAACAAGCTTGCTCCTTAGTTTAG

Upstream 100 bases:

>100_bases
AAGATCGAAATCAAGTGTTCTCCAGCCCCCAATCCGTTATCTTTTTTTTTTATAATCCCTAGTATAAACTGCTTTTTTCC
ATACTTTTTAGAGGACAATT

Downstream 100 bases:

>100_bases
TAGTAAATTTTCAGGGACAGTCGACATTGAAGCAAAACGTCCTCCAAGATTTTGGAGAACATCTGACCATAATTTGTCAG
GACGAGCAGTAAAAACGATC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MPHLFSKACQYFPGGVNSPVRACRSVDITPPVVTRASGDFFTDSQGKTYIDFCGSWGSLIHGHSHPYICEAIQQGLQRGC
SYGLTSEQEISFAEEIFSYLEISNDHKIRFMSTGSEATMTAVRLARGVTERPIIIKFSGCYHGHSDVFLQEIHFQQTILD
TVDLTQPLTLSLPFNNLSLFLDVMNQIGHRVAGVIFEPICANMGVVLPLPGFIEGIIQTCRKTGSLSIMDEVVTGFRVSK
GGAAALHPLKPDILVFGKILGGGLPASAVCAPAAIMDYLAPVGKVFQAGTLSGNPLAMSAGKASIALCREKNFYTQLSAI
EDDFLSPIEQMIQQSGIPVTLVRYGNLFSFFFSENRPNNLKEVQLCNTDIFRTFYQQAFLKGIYLSPSPFEASFLSTAHS
MENLNYAQQVLIESLEQACSLV

Sequences:

>Translated_422_residues
MPHLFSKACQYFPGGVNSPVRACRSVDITPPVVTRASGDFFTDSQGKTYIDFCGSWGSLIHGHSHPYICEAIQQGLQRGC
SYGLTSEQEISFAEEIFSYLEISNDHKIRFMSTGSEATMTAVRLARGVTERPIIIKFSGCYHGHSDVFLQEIHFQQTILD
TVDLTQPLTLSLPFNNLSLFLDVMNQIGHRVAGVIFEPICANMGVVLPLPGFIEGIIQTCRKTGSLSIMDEVVTGFRVSK
GGAAALHPLKPDILVFGKILGGGLPASAVCAPAAIMDYLAPVGKVFQAGTLSGNPLAMSAGKASIALCREKNFYTQLSAI
EDDFLSPIEQMIQQSGIPVTLVRYGNLFSFFFSENRPNNLKEVQLCNTDIFRTFYQQAFLKGIYLSPSPFEASFLSTAHS
MENLNYAQQVLIESLEQACSLV
>Mature_421_residues
PHLFSKACQYFPGGVNSPVRACRSVDITPPVVTRASGDFFTDSQGKTYIDFCGSWGSLIHGHSHPYICEAIQQGLQRGCS
YGLTSEQEISFAEEIFSYLEISNDHKIRFMSTGSEATMTAVRLARGVTERPIIIKFSGCYHGHSDVFLQEIHFQQTILDT
VDLTQPLTLSLPFNNLSLFLDVMNQIGHRVAGVIFEPICANMGVVLPLPGFIEGIIQTCRKTGSLSIMDEVVTGFRVSKG
GAAALHPLKPDILVFGKILGGGLPASAVCAPAAIMDYLAPVGKVFQAGTLSGNPLAMSAGKASIALCREKNFYTQLSAIE
DDFLSPIEQMIQQSGIPVTLVRYGNLFSFFFSENRPNNLKEVQLCNTDIFRTFYQQAFLKGIYLSPSPFEASFLSTAHSM
ENLNYAQQVLIESLEQACSLV

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=298, Percent_Identity=30.2013422818792, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI24119277, Length=323, Percent_Identity=24.4582043343653, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI284507298, Length=213, Percent_Identity=31.924882629108, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI37574042, Length=310, Percent_Identity=23.5483870967742, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1786349, Length=411, Percent_Identity=38.6861313868613, Blast_Score=307, Evalue=7e-85,
Organism=Escherichia coli, GI1787560, Length=273, Percent_Identity=32.6007326007326, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1789759, Length=309, Percent_Identity=29.126213592233, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI145693181, Length=329, Percent_Identity=29.1793313069909, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1789016, Length=360, Percent_Identity=26.1111111111111, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788044, Length=322, Percent_Identity=26.3975155279503, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786991, Length=315, Percent_Identity=26.031746031746, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144271, Length=354, Percent_Identity=27.683615819209, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25144274, Length=180, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=369, Percent_Identity=25.7452574525745, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=313, Percent_Identity=24.2811501597444, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_CHLMU (Q9PKI3)

Other databases:

- EMBL:   AE002160
- PIR:   B81697
- RefSeq:   NP_296859.1
- ProteinModelPortal:   Q9PKI3
- SMR:   Q9PKI3
- GeneID:   1245840
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0482
- TIGR:   TC_0482
- HOGENOM:   HBG725944
- OMA:   VENYEQA
- ProtClustDB:   PRK00615
- BioCyc:   CMUR243161:TC_0482-MONOMER
- BRENDA:   5.4.3.8
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46159; Mature: 46027

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHLFSKACQYFPGGVNSPVRACRSVDITPPVVTRASGDFFTDSQGKTYIDFCGSWGSLI
CCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCEECCCCCCEECCCCCEEEEECCCHHHHH
HGHSHPYICEAIQQGLQRGCSYGLTSEQEISFAEEIFSYLEISNDHKIRFMSTGSEATMT
CCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
AVRLARGVTERPIIIKFSGCYHGHSDVFLQEIHFQQTILDTVDLTQPLTLSLPFNNLSLF
HHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHH
LDVMNQIGHRVAGVIFEPICANMGVVLPLPGFIEGIIQTCRKTGSLSIMDEVVTGFRVSK
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHCEEECC
GGAAALHPLKPDILVFGKILGGGLPASAVCAPAAIMDYLAPVGKVFQAGTLSGNPLAMSA
CCCCEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
GKASIALCREKNFYTQLSAIEDDFLSPIEQMIQQSGIPVTLVRYGNLFSFFFSENRPNNL
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHEEHCCCCCCCCC
KEVQLCNTDIFRTFYQQAFLKGIYLSPSPFEASFLSTAHSMENLNYAQQVLIESLEQACS
CCEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LV
CC
>Mature Secondary Structure 
PHLFSKACQYFPGGVNSPVRACRSVDITPPVVTRASGDFFTDSQGKTYIDFCGSWGSLI
CCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCEECCCCCCEECCCCCEEEEECCCHHHHH
HGHSHPYICEAIQQGLQRGCSYGLTSEQEISFAEEIFSYLEISNDHKIRFMSTGSEATMT
CCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
AVRLARGVTERPIIIKFSGCYHGHSDVFLQEIHFQQTILDTVDLTQPLTLSLPFNNLSLF
HHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHH
LDVMNQIGHRVAGVIFEPICANMGVVLPLPGFIEGIIQTCRKTGSLSIMDEVVTGFRVSK
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHCEEECC
GGAAALHPLKPDILVFGKILGGGLPASAVCAPAAIMDYLAPVGKVFQAGTLSGNPLAMSA
CCCCEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
GKASIALCREKNFYTQLSAIEDDFLSPIEQMIQQSGIPVTLVRYGNLFSFFFSENRPNNL
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHEEHCCCCCCCCC
KEVQLCNTDIFRTFYQQAFLKGIYLSPSPFEASFLSTAHSMENLNYAQQVLIESLEQACS
CCEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LV
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935