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The map label for this gene is pfkA-2

Identifier: 15835097

GI number: 15835097

Start: 581135

End: 582781

Strand: Direct

Name: pfkA-2

Synonym: TC0479

Alternate gene names: 15835097

Gene position: 581135-582781 (Clockwise)

Preceding gene: 15835096

Following gene: 15835100

Centisome position: 54.16

GC content: 38.43

Gene sequence:

>1647_bases
ATGGAGTTACTCTCTGTAAATAAGAGTTATTTTGAACTACAAAGATTACATTATCGTCCAGTCACTCTAGCATTGTTGGA
TAATTTATGTTCTCTTCATATTAAAGAATCAGCTTCTTCTGAACCTAATCCAGGTTTATTAGCCAAGCATATTCCACATC
TATGTGCCCTTCCTAATTTAACGATTCAAAAAGGGGATCGCTCTTCTTCTGAGCCTTTACGTATCGGAGTTTTACTTTCA
GGAGGACAAGCACCAGGAGGTCATAATGTAGTCATAGGGTTATTTGAAGGACTACGAGCTTTTAATAAAGAAACTAAACT
TTTTGGATTTATTAAAGGCCCCTTGGGGCTTATTAGAGGGCTATATAAGGACCTAGACATCTCAGTTATCTATGATTATT
ACAACGCTGGTGGGTTTGACATGCTTTCCTCCAGTAAAGAGAAAATCAAAACTAAAGAGCAAAAGAGTGCTATTCTCTCT
ACCGTAAAAAAAATGAAACTACACGGGCTGCTTATTGTGGGCGGAGATAACTCCAATACCGATACAGCAATGCTAGCAGA
ATATTTTATCGAGCACAACTGTCCTACGGCTGTAATTGGAGTTCCTAAAACCATTGATGGAGATTTGAAAAACTCTTGGA
TCGAAACCCCTCTAGGATTCCATACATCATGCCGAACTTATTCAGAAATGATTGGAAATTTGGAAAAAGATATTCTGTCC
ACTCGGAAATATCATCATTTTGTCAAATTAATGGGGGAACAAGCCTCGCACAGCACTTTGGAGTGCGGTCTTCAAGCTCT
TCCTAATATTACTCTAATAGGAGAGGAAGTAGCTATTCACCACACATCTTTAAGAAGTTTAAGTCATCATATTGCACGAG
GACTTATTAAACGGTTCCGTAAAGATAAAAACTATAGCACGATTCTTATCCCAGAAGGTTTGATCAAACAGATCCCTGAC
ACAAAACTGTTAATTCATGAATTAAACGCTCTGATAGCTGAAGGACAATTTTCGGTTAATCATTTTGACCAACAATTATC
GCCTATGGCTCTTCAAACTTTCTCTTCGCTTCCAGAAAATATTCGCGAGCAGCTACTTCTTGACAGAGACTCGTATGGAA
ATGTTCGCGTGTCTAAAATTGCTATAGAAGAGCTGCTAGCATCTTTAGTTAGTGATGAAATAGCTAAACTAGAACCAACT
ATGCCCTTTGCTCCTGTAACACATTTTTTAGGCTATGAATCGCGCGCGAGTTTTCCTTCAAATTTTGATTCAAATTATGG
TTTAGCCTTAGGAATTGCAGCTTCTCTTTTCTTAGTAAGAGGTAAAACTGGATACATGGTGACTATAGGCAACTTAGCGG
AAGAATATGCTCACTGGACAATAGCAGCAACTCCCCTATACAAAATGATGCATTTAGAAAAACGGTTTAATGAAGAGACA
CCAGTAATTAAGACAGATTCTGTATCTCCACACTCCCCCATGGTGCAATACCTGCACAAAATAAAAGAGGCCTGCCTGCT
AGAAGATTTATACCGATTTCCTGGACCTTTGCAATACTTTGAAGAGCAAGCACTAATTGACCAACGCCCACTAACACTTC
TTTGGGAAAAAGGCGCTTTATCAGAAAACAATGCTAACAGATTATAA

Upstream 100 bases:

>100_bases
CTTATCGTATCTATCCTAAAATGAATCATCATCTTTGCGTGTATTCTGAGGCTTTTCAAGACCTTATCAATTGGCTAAAA
CATCAATTATTAGGGACACC

Downstream 100 bases:

>100_bases
ATAAAAGAAATAATTAGATGAGGAGATAGCGGTAGTCGGACTTGAACCAACGACACGCGGATTATGATTCCGCTGCTCTA
ACCACCTGAGCTATACCGCC

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MELLSVNKSYFELQRLHYRPVTLALLDNLCSLHIKESASSEPNPGLLAKHIPHLCALPNLTIQKGDRSSSEPLRIGVLLS
GGQAPGGHNVVIGLFEGLRAFNKETKLFGFIKGPLGLIRGLYKDLDISVIYDYYNAGGFDMLSSSKEKIKTKEQKSAILS
TVKKMKLHGLLIVGGDNSNTDTAMLAEYFIEHNCPTAVIGVPKTIDGDLKNSWIETPLGFHTSCRTYSEMIGNLEKDILS
TRKYHHFVKLMGEQASHSTLECGLQALPNITLIGEEVAIHHTSLRSLSHHIARGLIKRFRKDKNYSTILIPEGLIKQIPD
TKLLIHELNALIAEGQFSVNHFDQQLSPMALQTFSSLPENIREQLLLDRDSYGNVRVSKIAIEELLASLVSDEIAKLEPT
MPFAPVTHFLGYESRASFPSNFDSNYGLALGIAASLFLVRGKTGYMVTIGNLAEEYAHWTIAATPLYKMMHLEKRFNEET
PVIKTDSVSPHSPMVQYLHKIKEACLLEDLYRFPGPLQYFEEQALIDQRPLTLLWEKGALSENNANRL

Sequences:

>Translated_548_residues
MELLSVNKSYFELQRLHYRPVTLALLDNLCSLHIKESASSEPNPGLLAKHIPHLCALPNLTIQKGDRSSSEPLRIGVLLS
GGQAPGGHNVVIGLFEGLRAFNKETKLFGFIKGPLGLIRGLYKDLDISVIYDYYNAGGFDMLSSSKEKIKTKEQKSAILS
TVKKMKLHGLLIVGGDNSNTDTAMLAEYFIEHNCPTAVIGVPKTIDGDLKNSWIETPLGFHTSCRTYSEMIGNLEKDILS
TRKYHHFVKLMGEQASHSTLECGLQALPNITLIGEEVAIHHTSLRSLSHHIARGLIKRFRKDKNYSTILIPEGLIKQIPD
TKLLIHELNALIAEGQFSVNHFDQQLSPMALQTFSSLPENIREQLLLDRDSYGNVRVSKIAIEELLASLVSDEIAKLEPT
MPFAPVTHFLGYESRASFPSNFDSNYGLALGIAASLFLVRGKTGYMVTIGNLAEEYAHWTIAATPLYKMMHLEKRFNEET
PVIKTDSVSPHSPMVQYLHKIKEACLLEDLYRFPGPLQYFEEQALIDQRPLTLLWEKGALSENNANRL
>Mature_548_residues
MELLSVNKSYFELQRLHYRPVTLALLDNLCSLHIKESASSEPNPGLLAKHIPHLCALPNLTIQKGDRSSSEPLRIGVLLS
GGQAPGGHNVVIGLFEGLRAFNKETKLFGFIKGPLGLIRGLYKDLDISVIYDYYNAGGFDMLSSSKEKIKTKEQKSAILS
TVKKMKLHGLLIVGGDNSNTDTAMLAEYFIEHNCPTAVIGVPKTIDGDLKNSWIETPLGFHTSCRTYSEMIGNLEKDILS
TRKYHHFVKLMGEQASHSTLECGLQALPNITLIGEEVAIHHTSLRSLSHHIARGLIKRFRKDKNYSTILIPEGLIKQIPD
TKLLIHELNALIAEGQFSVNHFDQQLSPMALQTFSSLPENIREQLLLDRDSYGNVRVSKIAIEELLASLVSDEIAKLEPT
MPFAPVTHFLGYESRASFPSNFDSNYGLALGIAASLFLVRGKTGYMVTIGNLAEEYAHWTIAATPLYKMMHLEKRFNEET
PVIKTDSVSPHSPMVQYLHKIKEACLLEDLYRFPGPLQYFEEQALIDQRPLTLLWEKGALSENNANRL

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=221, Percent_Identity=27.6018099547511, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 61219; Mature: 61219

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLSVNKSYFELQRLHYRPVTLALLDNLCSLHIKESASSEPNPGLLAKHIPHLCALPNL
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCCCCHHHHHCCHHHCCCCC
TIQKGDRSSSEPLRIGVLLSGGQAPGGHNVVIGLFEGLRAFNKETKLFGFIKGPLGLIRG
EEECCCCCCCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHHCCCCCEEEEEHHHHHHHHH
LYKDLDISVIYDYYNAGGFDMLSSSKEKIKTKEQKSAILSTVKKMKLHGLLIVGGDNSNT
HHHCCCEEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCC
DTAMLAEYFIEHNCPTAVIGVPKTIDGDLKNSWIETPLGFHTSCRTYSEMIGNLEKDILS
HHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
TRKYHHFVKLMGEQASHSTLECGLQALPNITLIGEEVAIHHTSLRSLSHHIARGLIKRFR
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
KDKNYSTILIPEGLIKQIPDTKLLIHELNALIAEGQFSVNHFDQQLSPMALQTFSSLPEN
CCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHH
IREQLLLDRDSYGNVRVSKIAIEELLASLVSDEIAKLEPTMPFAPVTHFLGYESRASFPS
HHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCC
NFDSNYGLALGIAASLFLVRGKTGYMVTIGNLAEEYAHWTIAATPLYKMMHLEKRFNEET
CCCCCCCHHHHHHHHHHHEECCCCEEEEEHHHHHHHHCEEEEHHHHHHHHHHHHHCCCCC
PVIKTDSVSPHSPMVQYLHKIKEACLLEDLYRFPGPLQYFEEQALIDQRPLTLLWEKGAL
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCC
SENNANRL
CCCCCCCC
>Mature Secondary Structure
MELLSVNKSYFELQRLHYRPVTLALLDNLCSLHIKESASSEPNPGLLAKHIPHLCALPNL
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCCCCHHHHHCCHHHCCCCC
TIQKGDRSSSEPLRIGVLLSGGQAPGGHNVVIGLFEGLRAFNKETKLFGFIKGPLGLIRG
EEECCCCCCCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHHCCCCCEEEEEHHHHHHHHH
LYKDLDISVIYDYYNAGGFDMLSSSKEKIKTKEQKSAILSTVKKMKLHGLLIVGGDNSNT
HHHCCCEEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCC
DTAMLAEYFIEHNCPTAVIGVPKTIDGDLKNSWIETPLGFHTSCRTYSEMIGNLEKDILS
HHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
TRKYHHFVKLMGEQASHSTLECGLQALPNITLIGEEVAIHHTSLRSLSHHIARGLIKRFR
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
KDKNYSTILIPEGLIKQIPDTKLLIHELNALIAEGQFSVNHFDQQLSPMALQTFSSLPEN
CCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHH
IREQLLLDRDSYGNVRVSKIAIEELLASLVSDEIAKLEPTMPFAPVTHFLGYESRASFPS
HHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCC
NFDSNYGLALGIAASLFLVRGKTGYMVTIGNLAEEYAHWTIAATPLYKMMHLEKRFNEET
CCCCCCCHHHHHHHHHHHEECCCCEEEEEHHHHHHHHCEEEEHHHHHHHHHHHHHCCCCC
PVIKTDSVSPHSPMVQYLHKIKEACLLEDLYRFPGPLQYFEEQALIDQRPLTLLWEKGAL
CEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCC
SENNANRL
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA