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The map label for this gene is leuS

Identifier: 15835099

GI number: 15835099

Start: 584289

End: 586748

Strand: Reverse

Name: leuS

Synonym: TC0481

Alternate gene names: 15835099

Gene position: 586748-584289 (Counterclockwise)

Preceding gene: 15835101

Following gene: 15835098

Centisome position: 54.69

GC content: 41.75

Gene sequence:

>2460_bases
ATGCGCTATGATCCCAGTCTCATAGAAGAGAAGTGGCAAAAGTTTTGGAAAGAAGAACAGACTTTTAGAGCCGAAGAGGA
TGAGACTAAAACAAAATACTATGTTTTAGATATGTTCCCTTATCCATCAGGGGCGGGATTGCATGTTGGGCACTTGATTG
GTTATACGGCGACAGATATTGTTGCGAGATACAAGCGTGCTAAAGGATTTTCTGTTTTGCATCCTATGGGATGGGATAGC
TTTGGGCTACCTGCCGAGCAGTATGCTATACGTACAGGGACGCATCCAAGAGAAACCACGGAGAAGAATATTGCTAATTT
TAAACAGCAGTTGACTTCAATGGGATTTTCCTACGATGAAAGCCGCGAATTTGCTACTTCGGATCCAGAATACTATAAAT
GGACACAAAAGCTTTTTTTGATCCTTTATGAGAAAGGTCTAGCCTATATGGCTGATATGGCCGTTAACTACTGTCCTGAG
TTGGGCACTGTTTTGTCTAATGAAGAAGTAGAAAATGGTTTTTCTGTTGAAGGAGGATATCCTGTTGAAAGAAGGATGCT
CCGCCAGTGGGTATTACGTATAACAGCTTTTGCGGATCAGTTATTAGGTGGATTAGATGAGCTAGATTGGCCTGAAAGTG
TTAAACAGCTTCAGAGAAATTGGATCGGGAAATCCGTAGGAGCATCTGTCCATTTTGAAACAGAACACGGTGTTCTGGAA
GTTTTTACAACAAGACCAGATACTCTGATAGGGGTTTCTTTTTTAGTGTTGGCTCCCGAGCATCCTTTGGTTGATCTTTT
AACTTCTGATGAGCAGAAAACTATTGTGGCTCAATATGTCAAGGAGACGCAAAGTAAAAGTGAAAGAGATCGCATCAGTG
AAATGAAAACGAAAAGCGGGGTCTTTACTGGAGCTTATGCGAAACATCCGGTTACTCAAAATCCTATTCCTATTTGGATT
GCGGATTATGTGTTAATGGGATACGGTTCAGGCGCAGTTATGGGAGTTCCTGCTCATGACGACCGAGATTTATTATTCGC
GCAACAGTTCGATTTACCGATAATCTCTGTTGTTAGTGAAGATGGCGTCTGTATTAATAGTTGCCATGAGGATTTTAGTC
TGGATGGGCTATCAGGAGAAGAAGCTAAGCAGTATGTGATTAACTTTTTAGAAAAGAATAATTTAGGTTCTGCAAAGGTT
GCGTATAAGCTGCGAGATTGGTTGTTCTCTCGTCAACGCTATTGGGGAGAACCGATTCCAGTCATCCATTTTGAAGATGG
TTCATGTCGTCCTTTAAAAGACGATGAGCTTCCTTTACTTCCCCCTGAAATCCAGGATTATCGGCCTGAGGGGGTTGGCC
AGGGTCCTCTAGCCAAAGTAAAAGAATGGGTGAATGTTTTTGATTCAGAGACGCAAAAAGAAGGTAAGCGTGAAACGCAT
ACCATGCCTCAGTGGGCTGGCTCTTGCTGGTACTATTTGCGTTTTTGTGATGCTCATAACTCCTGTGCTCCTTGGGCAGA
GGAAAAAGAACAGTACTGGATGCCAGTAGATCTTTATATTGGAGGGGCTGAACATGCTGTGTTGCACCTGTTATACGCAC
GTTTTTGGCATCAGGTATTTTATGAAGCCGGCATTGTTTCTACTCCCGAGCCATTCAAAAAATTAGTCAATCAGGGGTTA
GTTTTATCAACATCCTATCGTATTCCTGGGAAAGGATATATAGCTCCCGAAATGGCTAAAGAAGAGAATGGGCAATGGAT
TTCTCCTTCAGGGGAATTACTTGACGTTCGCCAAGAAAAAATGTCGAAATCTAAGTTGAATGGAGTAGATCCAAAGGTTT
TGATAGATGAATTCGGAGCAGATGCGGTTCGGATGTACGCCATGTTTTCTGGACCATTAGATAAAAATAAACTCTGGTCG
AACCAGGGGGTTGCTGGCTGCCGACGATTTTTAAACCGTTTTTATGAAATGGCAACTTCTTCTCGGGTAAAAGACGAGGA
TATTTTTGAGGGAATGTCCTTAGCACACAAACTTGTGCAACGTGTAACGGATGACATTGAAAAATTGTCTTTGAATACGA
TAACGTCTTCTTTTATGGAGTTTATCAATGAATTTGTAAAACTTCCTGTATATCCAAAAAATGCTGTAGAAATGGCTGTA
CGAGCTTTAGCTCCCATTGCTCCTCATATTAGTGAAGAGTTATGGGTGTTGTTAGGGAATGCTTCGGGTATTGAAAAGGC
TGGTTGGCCTAAAGCACTCCCCGAGTATTTAGAGGGGAAAATCGTGACCATCGTAGTCCAAGTAAATGGGAAGCTTCGAG
CTCGATTAGATATTAGTAAGGATGCAATAGAGGAAGAGGTTGTTGCATTGGCTAAAGAAGCTGTATCTAAATATTTGGAA
GGTGGGGTAGTGAGAAAGACTATTTTTGTTCTGAACCGACTAGTTAACTTCGTGATATAA

Upstream 100 bases:

>100_bases
CGATCTTAAATATAGTGTATGTTTAGCGTTTATTTTTTCCTCATAGCAAGTTGGTTGTGAAGAGGAGCGTGCCAATAAAG
ATAACAATAGGTTAGGTGCT

Downstream 100 bases:

>100_bases
TGAGACGTTGGCTAACTTCCCGTCTTTATGATGCTTTTTTAGTTGCTGCTTTCTTAGCAGCAGCACCACGCATTTTTTAT
AAAGTAGTCTTTCACGGGAA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS

Number of amino acids: Translated: 819; Mature: 819

Protein sequence:

>819_residues
MRYDPSLIEEKWQKFWKEEQTFRAEEDETKTKYYVLDMFPYPSGAGLHVGHLIGYTATDIVARYKRAKGFSVLHPMGWDS
FGLPAEQYAIRTGTHPRETTEKNIANFKQQLTSMGFSYDESREFATSDPEYYKWTQKLFLILYEKGLAYMADMAVNYCPE
LGTVLSNEEVENGFSVEGGYPVERRMLRQWVLRITAFADQLLGGLDELDWPESVKQLQRNWIGKSVGASVHFETEHGVLE
VFTTRPDTLIGVSFLVLAPEHPLVDLLTSDEQKTIVAQYVKETQSKSERDRISEMKTKSGVFTGAYAKHPVTQNPIPIWI
ADYVLMGYGSGAVMGVPAHDDRDLLFAQQFDLPIISVVSEDGVCINSCHEDFSLDGLSGEEAKQYVINFLEKNNLGSAKV
AYKLRDWLFSRQRYWGEPIPVIHFEDGSCRPLKDDELPLLPPEIQDYRPEGVGQGPLAKVKEWVNVFDSETQKEGKRETH
TMPQWAGSCWYYLRFCDAHNSCAPWAEEKEQYWMPVDLYIGGAEHAVLHLLYARFWHQVFYEAGIVSTPEPFKKLVNQGL
VLSTSYRIPGKGYIAPEMAKEENGQWISPSGELLDVRQEKMSKSKLNGVDPKVLIDEFGADAVRMYAMFSGPLDKNKLWS
NQGVAGCRRFLNRFYEMATSSRVKDEDIFEGMSLAHKLVQRVTDDIEKLSLNTITSSFMEFINEFVKLPVYPKNAVEMAV
RALAPIAPHISEELWVLLGNASGIEKAGWPKALPEYLEGKIVTIVVQVNGKLRARLDISKDAIEEEVVALAKEAVSKYLE
GGVVRKTIFVLNRLVNFVI

Sequences:

>Translated_819_residues
MRYDPSLIEEKWQKFWKEEQTFRAEEDETKTKYYVLDMFPYPSGAGLHVGHLIGYTATDIVARYKRAKGFSVLHPMGWDS
FGLPAEQYAIRTGTHPRETTEKNIANFKQQLTSMGFSYDESREFATSDPEYYKWTQKLFLILYEKGLAYMADMAVNYCPE
LGTVLSNEEVENGFSVEGGYPVERRMLRQWVLRITAFADQLLGGLDELDWPESVKQLQRNWIGKSVGASVHFETEHGVLE
VFTTRPDTLIGVSFLVLAPEHPLVDLLTSDEQKTIVAQYVKETQSKSERDRISEMKTKSGVFTGAYAKHPVTQNPIPIWI
ADYVLMGYGSGAVMGVPAHDDRDLLFAQQFDLPIISVVSEDGVCINSCHEDFSLDGLSGEEAKQYVINFLEKNNLGSAKV
AYKLRDWLFSRQRYWGEPIPVIHFEDGSCRPLKDDELPLLPPEIQDYRPEGVGQGPLAKVKEWVNVFDSETQKEGKRETH
TMPQWAGSCWYYLRFCDAHNSCAPWAEEKEQYWMPVDLYIGGAEHAVLHLLYARFWHQVFYEAGIVSTPEPFKKLVNQGL
VLSTSYRIPGKGYIAPEMAKEENGQWISPSGELLDVRQEKMSKSKLNGVDPKVLIDEFGADAVRMYAMFSGPLDKNKLWS
NQGVAGCRRFLNRFYEMATSSRVKDEDIFEGMSLAHKLVQRVTDDIEKLSLNTITSSFMEFINEFVKLPVYPKNAVEMAV
RALAPIAPHISEELWVLLGNASGIEKAGWPKALPEYLEGKIVTIVVQVNGKLRARLDISKDAIEEEVVALAKEAVSKYLE
GGVVRKTIFVLNRLVNFVI
>Mature_819_residues
MRYDPSLIEEKWQKFWKEEQTFRAEEDETKTKYYVLDMFPYPSGAGLHVGHLIGYTATDIVARYKRAKGFSVLHPMGWDS
FGLPAEQYAIRTGTHPRETTEKNIANFKQQLTSMGFSYDESREFATSDPEYYKWTQKLFLILYEKGLAYMADMAVNYCPE
LGTVLSNEEVENGFSVEGGYPVERRMLRQWVLRITAFADQLLGGLDELDWPESVKQLQRNWIGKSVGASVHFETEHGVLE
VFTTRPDTLIGVSFLVLAPEHPLVDLLTSDEQKTIVAQYVKETQSKSERDRISEMKTKSGVFTGAYAKHPVTQNPIPIWI
ADYVLMGYGSGAVMGVPAHDDRDLLFAQQFDLPIISVVSEDGVCINSCHEDFSLDGLSGEEAKQYVINFLEKNNLGSAKV
AYKLRDWLFSRQRYWGEPIPVIHFEDGSCRPLKDDELPLLPPEIQDYRPEGVGQGPLAKVKEWVNVFDSETQKEGKRETH
TMPQWAGSCWYYLRFCDAHNSCAPWAEEKEQYWMPVDLYIGGAEHAVLHLLYARFWHQVFYEAGIVSTPEPFKKLVNQGL
VLSTSYRIPGKGYIAPEMAKEENGQWISPSGELLDVRQEKMSKSKLNGVDPKVLIDEFGADAVRMYAMFSGPLDKNKLWS
NQGVAGCRRFLNRFYEMATSSRVKDEDIFEGMSLAHKLVQRVTDDIEKLSLNTITSSFMEFINEFVKLPVYPKNAVEMAV
RALAPIAPHISEELWVLLGNASGIEKAGWPKALPEYLEGKIVTIVVQVNGKLRARLDISKDAIEEEVVALAKEAVSKYLE
GGVVRKTIFVLNRLVNFVI

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7661872, Length=867, Percent_Identity=32.8719723183391, Blast_Score=454, Evalue=1e-127,
Organism=Homo sapiens, GI94721239, Length=200, Percent_Identity=27.5, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI94721241, Length=200, Percent_Identity=27.5, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1786861, Length=870, Percent_Identity=40.6896551724138, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1790708, Length=378, Percent_Identity=25.1322751322751, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71997517, Length=838, Percent_Identity=28.4009546539379, Blast_Score=284, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI71997510, Length=841, Percent_Identity=28.2996432818074, Blast_Score=284, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI212645227, Length=339, Percent_Identity=27.7286135693215, Blast_Score=116, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323414, Length=886, Percent_Identity=35.5530474040632, Blast_Score=468, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6321531, Length=418, Percent_Identity=26.3157894736842, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21355409, Length=865, Percent_Identity=32.1387283236994, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17864482, Length=417, Percent_Identity=23.5011990407674, Blast_Score=94, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24653289, Length=417, Percent_Identity=23.5011990407674, Blast_Score=94, Evalue=5e-19,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYL_CHLMU (Q9PKI4)

Other databases:

- EMBL:   AE002160
- PIR:   G81698
- RefSeq:   NP_296858.1
- ProteinModelPortal:   Q9PKI4
- SMR:   Q9PKI4
- GeneID:   1245839
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0481
- TIGR:   TC_0481
- HOGENOM:   HBG693845
- OMA:   ERGNFPV
- ProtClustDB:   PRK00390
- BioCyc:   CMUR243161:TC_0481-MONOMER
- BRENDA:   6.1.1.4
- GO:   GO:0005737
- HAMAP:   MF_00049_B
- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF7
- PRINTS:   PR00985
- TIGRFAMs:   TIGR00396

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; SSF47323 tRNAsyn_1a_bind; SSF50677 ValRS_IleRS_edit

EC number: =6.1.1.4

Molecular weight: Translated: 93016; Mature: 93016

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 603-603

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYDPSLIEEKWQKFWKEEQTFRAEEDETKTKYYVLDMFPYPSGAGLHVGHLIGYTATDI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
VARYKRAKGFSVLHPMGWDSFGLPAEQYAIRTGTHPRETTEKNIANFKQQLTSMGFSYDE
HHHHHHCCCCEEECCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
SREFATSDPEYYKWTQKLFLILYEKGLAYMADMAVNYCPELGTVLSNEEVENGFSVEGGY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHCCCCCCCCCC
PVERRMLRQWVLRITAFADQLLGGLDELDWPESVKQLQRNWIGKSVGASVHFETEHGVLE
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHCCCEEEEECCCCEEE
VFTTRPDTLIGVSFLVLAPEHPLVDLLTSDEQKTIVAQYVKETQSKSERDRISEMKTKSG
EEECCCCHHHCCEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VFTGAYAKHPVTQNPIPIWIADYVLMGYGSGAVMGVPAHDDRDLLFAQQFDLPIISVVSE
CEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCEEEHHHCCCCEEEEECC
DGVCINSCHEDFSLDGLSGEEAKQYVINFLEKNNLGSAKVAYKLRDWLFSRQRYWGEPIP
CCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VIHFEDGSCRPLKDDELPLLPPEIQDYRPEGVGQGPLAKVKEWVNVFDSETQKEGKRETH
EEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCHHHC
TMPQWAGSCWYYLRFCDAHNSCAPWAEEKEQYWMPVDLYIGGAEHAVLHLLYARFWHQVF
CCCHHHCCCEEEEEEECCCCCCCCCCHHHHCEECEEEEEECCHHHHHHHHHHHHHHHHHH
YEAGIVSTPEPFKKLVNQGLVLSTSYRIPGKGYIAPEMAKEENGQWISPSGELLDVRQEK
HHCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHCCCCCEECCCCCHHHHHHHH
MSKSKLNGVDPKVLIDEFGADAVRMYAMFSGPLDKNKLWSNQGVAGCRRFLNRFYEMATS
HHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
SRVKDEDIFEGMSLAHKLVQRVTDDIEKLSLNTITSSFMEFINEFVKLPVYPKNAVEMAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
RALAPIAPHISEELWVLLGNASGIEKAGWPKALPEYLEGKIVTIVVQVNGKLRARLDISK
HHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCEEEEEEEEECCCEEEEECCCH
DAIEEEVVALAKEAVSKYLEGGVVRKTIFVLNRLVNFVI
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRYDPSLIEEKWQKFWKEEQTFRAEEDETKTKYYVLDMFPYPSGAGLHVGHLIGYTATDI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
VARYKRAKGFSVLHPMGWDSFGLPAEQYAIRTGTHPRETTEKNIANFKQQLTSMGFSYDE
HHHHHHCCCCEEECCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
SREFATSDPEYYKWTQKLFLILYEKGLAYMADMAVNYCPELGTVLSNEEVENGFSVEGGY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHCCCCCCCCCC
PVERRMLRQWVLRITAFADQLLGGLDELDWPESVKQLQRNWIGKSVGASVHFETEHGVLE
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHCCCEEEEECCCCEEE
VFTTRPDTLIGVSFLVLAPEHPLVDLLTSDEQKTIVAQYVKETQSKSERDRISEMKTKSG
EEECCCCHHHCCEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VFTGAYAKHPVTQNPIPIWIADYVLMGYGSGAVMGVPAHDDRDLLFAQQFDLPIISVVSE
CEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCEEEHHHCCCCEEEEECC
DGVCINSCHEDFSLDGLSGEEAKQYVINFLEKNNLGSAKVAYKLRDWLFSRQRYWGEPIP
CCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VIHFEDGSCRPLKDDELPLLPPEIQDYRPEGVGQGPLAKVKEWVNVFDSETQKEGKRETH
EEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCHHHC
TMPQWAGSCWYYLRFCDAHNSCAPWAEEKEQYWMPVDLYIGGAEHAVLHLLYARFWHQVF
CCCHHHCCCEEEEEEECCCCCCCCCCHHHHCEECEEEEEECCHHHHHHHHHHHHHHHHHH
YEAGIVSTPEPFKKLVNQGLVLSTSYRIPGKGYIAPEMAKEENGQWISPSGELLDVRQEK
HHCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHCCCCCEECCCCCHHHHHHHH
MSKSKLNGVDPKVLIDEFGADAVRMYAMFSGPLDKNKLWSNQGVAGCRRFLNRFYEMATS
HHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
SRVKDEDIFEGMSLAHKLVQRVTDDIEKLSLNTITSSFMEFINEFVKLPVYPKNAVEMAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
RALAPIAPHISEELWVLLGNASGIEKAGWPKALPEYLEGKIVTIVVQVNGKLRARLDISK
HHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCEEEEEEEEECCCEEEEECCCH
DAIEEEVVALAKEAVSKYLEGGVVRKTIFVLNRLVNFVI
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935