Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 15834695

GI number: 15834695

Start: 80287

End: 81621

Strand: Reverse

Name: trmE

Synonym: TC0070

Alternate gene names: 15834695

Gene position: 81621-80287 (Counterclockwise)

Preceding gene: 15834696

Following gene: 15834694

Centisome position: 7.61

GC content: 41.87

Gene sequence:

>1335_bases
ATGCTTCAAAATGATACAATCGCTGCCATTGCAACTCCTCCGGGAGAAGGGAGTGTCGCCATTGTGCGCGTCTCTGGCCC
TGACGCTATTTCTATTTCTAATCGTATTTTTTCTGGCAATATTGCAGGCTATGCCTCGCATACAGCCCACCTTGGCACTG
TAGTTCATAATGAAGTTTGTATTGATCAAGCTTTAGTGTTGATTATGCGAACCCCCCGTTCTTTTACAGGTGAAGACATT
GTAGAATTCCAATGCCATGGAGGATACTTCGCTTGCTCTCAAATTTTGAATGCCTTGTTGGCGGAAGGGGCTCGCGCAGC
ACTTCCCGGAGAATTTTCACAACGAGCTTTTTTGAATGGGAAAATCGACCTAATTCAAGCAGAGGCTATACAACAGCTCA
TTTCAGCTGAAAATATTGATGCTTTTCATATTGCCCAAAATCAATTTCAAGGGCATACCTCACAAGCCATTTCCTCGATT
TCCTCCTTAATCATAGAAGCTTTAGCCTACATAGAAGTTTTAGCAGACTTCCCTGAAGAAGATATAGAAACGGAACGTTC
CCTTCCTGTGCAAAAAATCACAGAAGCTCTCACTATCACACAAGAGCTTCTTTCTAGCTTTGATGAGGGACAACGTCTTG
CTCAAGGGACAAGCATTGTTTTAGCAGGGCTTCCCAATGCAGGGAAATCTTCTATACTCAATGCTCTTACTCAAAAAAAT
CGAGCAATTGTTACCGACATACCAGGAACAACTCGCGATATTTTAGAAGAAAACTGGGTTTTGCAAGGGAAGAATCTTCG
TCTGATTGATTCTGCAGGTATTCGAGAAACCGAAAACATTGTTGAAAAAGAAGGAATAGAACGCGCTCGGCAGGCCATGA
GTCAAGCAGAGGGCATCCTCTGGGTTATGGATGCCTCTCAACCTGTTCCAGAATTTCCTGCCATCCTATACCAAAAGCCG
ACTATTTTACTTTGGAATAAATGCGATGTTGCATCTCCCCCTCAACTAGAGGTCCCTTTTCAGCAAATTTTTGTATCTGC
TAAAACTGGAAAAGGGTTTTCGGAATTGCGACAAGCATTAAACAAATGGCTCCACACCACACAATTAGGAAAATCTTCTA
AAGTCTTTTTAGTTTCTGCAAGGCATCACAGCTTGCTGCATGCTGTTCATACATGCTTAACAGATGCGTTAACAGGATTC
CAAGAACATCTCCCTAATGAATGCCTTGCATTAGATCTCAGACAAGCTTTACATTCTATAGGGAATCTATCTGGATCTGA
AGTCACTGAAAATGTTTTAGGAGAAATTTTCAGTAAATTTTGTATAGGGAAATAG

Upstream 100 bases:

>100_bases
CCTCGTACTCTCCCTAATCTACCTTTCTTGCTTAGAAAGTTCCTCCAAAAGTATAATAGTCTCCTTTTCAGCACTTTGTT
TAGCCTAAAGGCGCCTACTT

Downstream 100 bases:

>100_bases
CCGGATGAACAAGCTACGCGTACCATCCAAGCGAGCATTTATCTTAAACACCTTGAACCAACTGTTCCCCAATCCTGAAC
CTTCTTTAAAAGGATGGCAT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MLQNDTIAAIATPPGEGSVAIVRVSGPDAISISNRIFSGNIAGYASHTAHLGTVVHNEVCIDQALVLIMRTPRSFTGEDI
VEFQCHGGYFACSQILNALLAEGARAALPGEFSQRAFLNGKIDLIQAEAIQQLISAENIDAFHIAQNQFQGHTSQAISSI
SSLIIEALAYIEVLADFPEEDIETERSLPVQKITEALTITQELLSSFDEGQRLAQGTSIVLAGLPNAGKSSILNALTQKN
RAIVTDIPGTTRDILEENWVLQGKNLRLIDSAGIRETENIVEKEGIERARQAMSQAEGILWVMDASQPVPEFPAILYQKP
TILLWNKCDVASPPQLEVPFQQIFVSAKTGKGFSELRQALNKWLHTTQLGKSSKVFLVSARHHSLLHAVHTCLTDALTGF
QEHLPNECLALDLRQALHSIGNLSGSEVTENVLGEIFSKFCIGK

Sequences:

>Translated_444_residues
MLQNDTIAAIATPPGEGSVAIVRVSGPDAISISNRIFSGNIAGYASHTAHLGTVVHNEVCIDQALVLIMRTPRSFTGEDI
VEFQCHGGYFACSQILNALLAEGARAALPGEFSQRAFLNGKIDLIQAEAIQQLISAENIDAFHIAQNQFQGHTSQAISSI
SSLIIEALAYIEVLADFPEEDIETERSLPVQKITEALTITQELLSSFDEGQRLAQGTSIVLAGLPNAGKSSILNALTQKN
RAIVTDIPGTTRDILEENWVLQGKNLRLIDSAGIRETENIVEKEGIERARQAMSQAEGILWVMDASQPVPEFPAILYQKP
TILLWNKCDVASPPQLEVPFQQIFVSAKTGKGFSELRQALNKWLHTTQLGKSSKVFLVSARHHSLLHAVHTCLTDALTGF
QEHLPNECLALDLRQALHSIGNLSGSEVTENVLGEIFSKFCIGK
>Mature_444_residues
MLQNDTIAAIATPPGEGSVAIVRVSGPDAISISNRIFSGNIAGYASHTAHLGTVVHNEVCIDQALVLIMRTPRSFTGEDI
VEFQCHGGYFACSQILNALLAEGARAALPGEFSQRAFLNGKIDLIQAEAIQQLISAENIDAFHIAQNQFQGHTSQAISSI
SSLIIEALAYIEVLADFPEEDIETERSLPVQKITEALTITQELLSSFDEGQRLAQGTSIVLAGLPNAGKSSILNALTQKN
RAIVTDIPGTTRDILEENWVLQGKNLRLIDSAGIRETENIVEKEGIERARQAMSQAEGILWVMDASQPVPEFPAILYQKP
TILLWNKCDVASPPQLEVPFQQIFVSAKTGKGFSELRQALNKWLHTTQLGKSSKVFLVSARHHSLLHAVHTCLTDALTGF
QEHLPNECLALDLRQALHSIGNLSGSEVTENVLGEIFSKFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=465, Percent_Identity=35.4838709677419, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI193082993, Length=450, Percent_Identity=35.3333333333333, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI193082988, Length=497, Percent_Identity=32.9979879275654, Blast_Score=190, Evalue=2e-48,
Organism=Escherichia coli, GI2367268, Length=465, Percent_Identity=37.2043010752688, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI87082120, Length=159, Percent_Identity=34.5911949685535, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=461, Percent_Identity=31.8872017353579, Blast_Score=173, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323665, Length=499, Percent_Identity=29.4589178356713, Blast_Score=189, Evalue=8e-49,
Organism=Drosophila melanogaster, GI45550813, Length=485, Percent_Identity=28.8659793814433, Blast_Score=162, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_CHLMU (Q9PLM9)

Other databases:

- EMBL:   AE002160
- RefSeq:   NP_296454.1
- ProteinModelPortal:   Q9PLM9
- SMR:   Q9PLM9
- GeneID:   1245599
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0070
- TIGR:   TC_0070
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q9PLM9
- ProtClustDB:   PRK05291
- BioCyc:   CMUR243161:TC_0070-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48245; Mature: 48245

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 82-82 BINDING 121-121 BINDING 444-444

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQNDTIAAIATPPGEGSVAIVRVSGPDAISISNRIFSGNIAGYASHTAHLGTVVHNEVC
CCCCCCEEEEECCCCCCCEEEEEECCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHH
IDQALVLIMRTPRSFTGEDIVEFQCHGGYFACSQILNALLAEGARAALPGEFSQRAFLNG
HHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHCHHHCCC
KIDLIQAEAIQQLISAENIDAFHIAQNQFQGHTSQAISSISSLIIEALAYIEVLADFPEE
CEEEEHHHHHHHHHHHCCCCCEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
DIETERSLPVQKITEALTITQELLSSFDEGQRLAQGTSIVLAGLPNAGKSSILNALTQKN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCC
RAIVTDIPGTTRDILEENWVLQGKNLRLIDSAGIRETENIVEKEGIERARQAMSQAEGIL
CEEEECCCCCHHHHHHCCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEE
WVMDASQPVPEFPAILYQKPTILLWNKCDVASPPQLEVPFQQIFVSAKTGKGFSELRQAL
EEEECCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCCCCHHHHEEECCCCCCHHHHHHHH
NKWLHTTQLGKSSKVFLVSARHHSLLHAVHTCLTDALTGFQEHLPNECLALDLRQALHSI
HHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GNLSGSEVTENVLGEIFSKFCIGK
HCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLQNDTIAAIATPPGEGSVAIVRVSGPDAISISNRIFSGNIAGYASHTAHLGTVVHNEVC
CCCCCCEEEEECCCCCCCEEEEEECCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHH
IDQALVLIMRTPRSFTGEDIVEFQCHGGYFACSQILNALLAEGARAALPGEFSQRAFLNG
HHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHCHHHCCC
KIDLIQAEAIQQLISAENIDAFHIAQNQFQGHTSQAISSISSLIIEALAYIEVLADFPEE
CEEEEHHHHHHHHHHHCCCCCEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
DIETERSLPVQKITEALTITQELLSSFDEGQRLAQGTSIVLAGLPNAGKSSILNALTQKN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCC
RAIVTDIPGTTRDILEENWVLQGKNLRLIDSAGIRETENIVEKEGIERARQAMSQAEGIL
CEEEECCCCCHHHHHHCCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEE
WVMDASQPVPEFPAILYQKPTILLWNKCDVASPPQLEVPFQQIFVSAKTGKGFSELRQAL
EEEECCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCCCCHHHHEEECCCCCCHHHHHHHH
NKWLHTTQLGKSSKVFLVSARHHSLLHAVHTCLTDALTGFQEHLPNECLALDLRQALHSI
HHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GNLSGSEVTENVLGEIFSKFCIGK
HCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935