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The map label for this gene is argF [H]

Identifier: 158338712

GI number: 158338712

Start: 5680147

End: 5681085

Strand: Direct

Name: argF [H]

Synonym: AM1_5620

Alternate gene names: 158338712

Gene position: 5680147-5681085 (Clockwise)

Preceding gene: 158338711

Following gene: 158338713

Centisome position: 87.34

GC content: 50.37

Gene sequence:

>939_bases
ATGTCTACTGATACTGCAAGTCTAAAAGGAAGAGATCTGCTAAGTTTAGCAGACTTAACAAGCCCAGAAATTGATGGTTT
GTTGGAACTGGCGGCTCAAATCAAGGTGGGACAGGTCAAGCCCCATTGTCCCAAGGTCCTGGGGTTGCTATTTACCAAGG
CTTCTACCCGCACAAGGGTTAGTTTCTCCGTGGGCATGTACCGTTTAGGAGGACAGGTGATTGATTTGAGGCCGGATGCC
ACCCAGGTGGGTCGAGGAGAGCCGTTAGAAGATACGGCTAGGGTCTTAGATCGATACTTAGATGTTTTGGCGATTCGCAC
CTATGAACAGCAGGATTTAAACATCTTTGCTCAATACGCTTCAATACCTGTGATTAATGCCCTGACAGATTTAGAACATC
CCTGTCAGATCTTTGCGGATTTGTTGACCCTACGAGAAGCCTTTGGACAGCTAGAAGGCTTGACTCTTACCTATTTAGGG
GATGGCAACAATGTCGCCCATTCCTTGCTGTTGGGGTGTGCCCTAGTCGGAGTAAATATTCGAGTCGCGACTCCCCTGGA
CTATCAGCCTCTCCCTGATTTTGTTGAGAAAGCCAAGATACTGGCGGGCGATCGCAGTTCTGTACTGATCACCACCGATC
CCCAAAAAGCCGTCGATGGGGCTCATGCCCTCTATACCGATGTCTGGGCCAGTATGGGCCAAGAAGAGTTGGCCAGTAAT
CGGATTCCTATCTTTCAGCCCTATCAAGTGAACGAATCCCTTTTGACGAAAGCGGATCCTAATGCCATTGTCCTCCATTG
CTTGCCTGCCCATCGCGGAGAAGAAATTACAGCAGACGTGATGGAAGGGGCACAGTCCCGCGTGTGGGATCAGGCCGAGA
ATCGCTTACACACCCAGCAGGCCCTGCTAGCTAGCGTGTTAGGCGCTAAGATCGACTGA

Upstream 100 bases:

>100_bases
ATAGCTGGAAAGACAGGGCTGACTTGAATAAGGTATTCTGGATCTTTCCACTATTTTGGTGCCTTAGCTGTTGTAGTTCC
CCAAGCCTGATCTAGAGTCT

Downstream 100 bases:

>100_bases
GCGTTTGGCGATTAAGCCCCATCAATCCGTGAATGAGTAAGTCTTCGATTCAGCCAGCTTTAATTTCAGGGGTGGTCCCC
GATTCGATTGCAGCAGAAGT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSTDTASLKGRDLLSLADLTSPEIDGLLELAAQIKVGQVKPHCPKVLGLLFTKASTRTRVSFSVGMYRLGGQVIDLRPDA
TQVGRGEPLEDTARVLDRYLDVLAIRTYEQQDLNIFAQYASIPVINALTDLEHPCQIFADLLTLREAFGQLEGLTLTYLG
DGNNVAHSLLLGCALVGVNIRVATPLDYQPLPDFVEKAKILAGDRSSVLITTDPQKAVDGAHALYTDVWASMGQEELASN
RIPIFQPYQVNESLLTKADPNAIVLHCLPAHRGEEITADVMEGAQSRVWDQAENRLHTQQALLASVLGAKID

Sequences:

>Translated_312_residues
MSTDTASLKGRDLLSLADLTSPEIDGLLELAAQIKVGQVKPHCPKVLGLLFTKASTRTRVSFSVGMYRLGGQVIDLRPDA
TQVGRGEPLEDTARVLDRYLDVLAIRTYEQQDLNIFAQYASIPVINALTDLEHPCQIFADLLTLREAFGQLEGLTLTYLG
DGNNVAHSLLLGCALVGVNIRVATPLDYQPLPDFVEKAKILAGDRSSVLITTDPQKAVDGAHALYTDVWASMGQEELASN
RIPIFQPYQVNESLLTKADPNAIVLHCLPAHRGEEITADVMEGAQSRVWDQAENRLHTQQALLASVLGAKID
>Mature_311_residues
STDTASLKGRDLLSLADLTSPEIDGLLELAAQIKVGQVKPHCPKVLGLLFTKASTRTRVSFSVGMYRLGGQVIDLRPDAT
QVGRGEPLEDTARVLDRYLDVLAIRTYEQQDLNIFAQYASIPVINALTDLEHPCQIFADLLTLREAFGQLEGLTLTYLGD
GNNVAHSLLLGCALVGVNIRVATPLDYQPLPDFVEKAKILAGDRSSVLITTDPQKAVDGAHALYTDVWASMGQEELASNR
IPIFQPYQVNESLLTKADPNAIVLHCLPAHRGEEITADVMEGAQSRVWDQAENRLHTQQALLASVLGAKID

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:
GO:0004585: Ornithine carbamoyltransferase
GO:0005737: Ornithine carbamoyltransferase
GO:0006520: Ornithine carbamoyltransferase
GO:0006526: Ornithine carbamoyltransferase
GO:0006591: Ornithine carbamoyltransferase
GO:0008652: Ornithine carbamoyltransferase
GO:0009348: Ornithine carbamoyltransferase
GO:0016597: Ornithine carbamoyltransferase
GO:0016740: Ornithine carbamoyltransferase
GO:0016743: Ornithine carbamoyltransferase

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=311, Percent_Identity=41.8006430868167, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=28.2051282051282, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1790703, Length=329, Percent_Identity=38.6018237082067, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1786469, Length=335, Percent_Identity=38.2089552238806, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI2367364, Length=314, Percent_Identity=30.2547770700637, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI48994908, Length=370, Percent_Identity=25.4054054054054, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=327, Percent_Identity=31.1926605504587, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6322373, Length=308, Percent_Identity=33.1168831168831, Blast_Score=172, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6322331, Length=312, Percent_Identity=28.8461538461538, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24642586, Length=327, Percent_Identity=29.9694189602446, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 33978; Mature: 33847

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDTASLKGRDLLSLADLTSPEIDGLLELAAQIKVGQVKPHCPKVLGLLFTKASTRTRV
CCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEE
SFSVGMYRLGGQVIDLRPDATQVGRGEPLEDTARVLDRYLDVLAIRTYEQQDLNIFAQYA
EEEHHHHHHCCEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
SIPVINALTDLEHPCQIFADLLTLREAFGQLEGLTLTYLGDGNNVAHSLLLGCALVGVNI
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCEE
RVATPLDYQPLPDFVEKAKILAGDRSSVLITTDPQKAVDGAHALYTDVWASMGQEELASN
EEECCCCCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHCHHHHHHHHHHHHCCHHHHHCC
RIPIFQPYQVNESLLTKADPNAIVLHCLPAHRGEEITADVMEGAQSRVWDQAENRLHTQQ
CCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALLASVLGAKID
HHHHHHHCCCCC
>Mature Secondary Structure 
STDTASLKGRDLLSLADLTSPEIDGLLELAAQIKVGQVKPHCPKVLGLLFTKASTRTRV
CCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEE
SFSVGMYRLGGQVIDLRPDATQVGRGEPLEDTARVLDRYLDVLAIRTYEQQDLNIFAQYA
EEEHHHHHHCCEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
SIPVINALTDLEHPCQIFADLLTLREAFGQLEGLTLTYLGDGNNVAHSLLLGCALVGVNI
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCEE
RVATPLDYQPLPDFVEKAKILAGDRSSVLITTDPQKAVDGAHALYTDVWASMGQEELASN
EEECCCCCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHCHHHHHHHHHHHHCCHHHHHCC
RIPIFQPYQVNESLLTKADPNAIVLHCLPAHRGEEITADVMEGAQSRVWDQAENRLHTQQ
CCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALLASVLGAKID
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]