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The map label for this gene is kefA [H]

Identifier: 158338332

GI number: 158338332

Start: 5294187

End: 5296145

Strand: Reverse

Name: kefA [H]

Synonym: AM1_5228

Alternate gene names: 158338332

Gene position: 5296145-5294187 (Counterclockwise)

Preceding gene: 158338333

Following gene: 158338331

Centisome position: 81.43

GC content: 53.6

Gene sequence:

>1959_bases
TTGCCTATCTCTGAGATGCTGTATCGACGACCATTTCGGCCCGTTCAAGGCTTTCTAATTCTGTTGTTCAGTCTGACCCT
CTTCCTGACTGGATTTTCCCTGCCATCCCGAGCGGAGATTACCATTGGTCGCAGCTTCGATACCGCTCCAATTGTGCTAG
ATGGCAAAGTCCTGTTTCAAGTCAGCAAGCTCAGGGAGTTCTCGGCAAAAGATCGGGCTAAGCAGGCCAACGATAGCTTA
GCCCAAGCGATACGAGATTTTGCAGCAGCCCAGGATTCCCCTGGTGTTCCCACAGTGAAGGTGACGCCAGGCGCTTTACC
GACCTTGCGTTTAGCCGACCGTCACCTCTTAACGGTGACGGATCGCGATGTCATGTTGGGCCAGGATGCCATGGAACAAG
CCGTGGGGTGGCAGCGCACGATTAATCGGGCGATTGCCACGGGTCTGAAGGAACGCACCCCGAGCTATCGACGCCAAGCC
ATCCTCAAGAGTGGGCTGGGGGTGTTGCTAGCGATTCTCCTGCACTGCGGTATTCAATGGATCCGGCTGCGCTGGCGGCG
GCAGCAGCTGCGAACGATGCAGGATACGAAGCGCCCTCTGGCGCTATTGGGGTTGACGAGCCTTCAGCTCTTGGTTTGGG
TTGGGGTGGGTTACTATATTTGCGAACTATTCCCCCAATTCCGTACCCTGCTGTACTTGGTGTTACGGAGCTTTGACGAA
CCCATCTTGATCCTGAAAGAGCAGGAACAGGGATACGGCATTGTTGACCTGTTGATTGTGGTGGGATTGATTCTGGGGTT
GTGGGTGATTGTACGGGGGTTGACACTGCTACTCAAGTCGAGAATCTTGCCGCTCTTGGGTGTGAATCGGGGATTGCAAG
ATGCGATCGCAATTCTCACCCAATATGTCCTCACCGGGTTGGGAGTGGTAGTCATCCTCCAAGTGTTGGGGGTGGATATC
GGTGCCCTGCTTATTGTCGGCAGTGCCCTAGGTTTGGGCATTGGCTTTGGTCTCCAAAATATCGTCAGCAACTTTATTAG
CGGTATCATCCTGCTTCTGGATCGCCCCATCGAAGCCGGAGATTTCATCAAGGTGGGAGAGCTAGTGGGTTCCGTCGAGA
AAATTGGGACCCGAAGCACCGAACTGCGCACCCACGATGAAGTGACGATCATCGTCCCCAACTCCCATTTTTTAGAAAAA
GAAGTGGTGAACTGGAGCCATAGCAGCCCCGTCTCCCGCCTACACCTGCCCGTGGACGTGGCCTATGGTTGTGATGTGGA
TCGCGTCCAAACGGCCCTGATTGAATCCGTCAGCCATCATCCAGAAGTTCTGGCCCATCCTCAACCCCAAGTTCAGTTTC
TGGGGTTTGGAGAGAGTGCCCTGAACTTTGAAGTCTTGGTATGGTTTCGTGAACCTCGGCAGCAGTATCGTCTAAAAAGC
GATTTGTATTTCCGTATCGAAGCTATGCTCCGCCAGTACCAGATCGAAATTCCGTTTCCCCAGCGGGATTTGCATGTGCG
ATCGCCCCATCTAGAGCAGCTGATCGATTTGCTCGTGCAGCAGCGGACAGAGAATGCCCCTCATTTGTACTATCCAGACT
CCGTGAGATCGCAACCGCCATCCCAACCGATCCTGGCTTCCCCCCCACCTAGCCCTCAGCCCATCTCCCGGCCATCCGCT
CCCCAAATCAACCTGCCAGAACTCGTGGCCCAGATGAAAGGAGAACAGGGACTGGATATTCAAGATCGTCGTCATCGGCT
GAATATTTATCCCCAATGTTTTGTGGGCTCGGAAGCTGTGATTTGGCTGATGCGGACTCAACGAGCCACCCGCAAAGAAG
CCCTACGCATTGGTCGGCTTCTGATGCGAAAACGGATTATTCATCACGTTTTGGATGAGCATCCCTTTGATGATGCCTAC
CTGTTTTATCGCTTTTATGCCGATGAGCAGCAGGAATAA

Upstream 100 bases:

>100_bases
CGCTGGTGTAACAGTTCGACGTCAGCACACCATTCCAAAAAGATCAGCATGATTTCAGACTCTTATCATGTCACCCTTGA
ATAATGGGTGAGGTGCAAGT

Downstream 100 bases:

>100_bases
CGTGGCAATAATGTCCTTTGCTCTTGCCAATAGCCAGTAGAAATATCTAGATATCTGGGGCGGAAATGCGGATCACCCCA
GAAAATCGATAGTTTTACTG

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 652; Mature: 651

Protein sequence:

>652_residues
MPISEMLYRRPFRPVQGFLILLFSLTLFLTGFSLPSRAEITIGRSFDTAPIVLDGKVLFQVSKLREFSAKDRAKQANDSL
AQAIRDFAAAQDSPGVPTVKVTPGALPTLRLADRHLLTVTDRDVMLGQDAMEQAVGWQRTINRAIATGLKERTPSYRRQA
ILKSGLGVLLAILLHCGIQWIRLRWRRQQLRTMQDTKRPLALLGLTSLQLLVWVGVGYYICELFPQFRTLLYLVLRSFDE
PILILKEQEQGYGIVDLLIVVGLILGLWVIVRGLTLLLKSRILPLLGVNRGLQDAIAILTQYVLTGLGVVVILQVLGVDI
GALLIVGSALGLGIGFGLQNIVSNFISGIILLLDRPIEAGDFIKVGELVGSVEKIGTRSTELRTHDEVTIIVPNSHFLEK
EVVNWSHSSPVSRLHLPVDVAYGCDVDRVQTALIESVSHHPEVLAHPQPQVQFLGFGESALNFEVLVWFREPRQQYRLKS
DLYFRIEAMLRQYQIEIPFPQRDLHVRSPHLEQLIDLLVQQRTENAPHLYYPDSVRSQPPSQPILASPPPSPQPISRPSA
PQINLPELVAQMKGEQGLDIQDRRHRLNIYPQCFVGSEAVIWLMRTQRATRKEALRIGRLLMRKRIIHHVLDEHPFDDAY
LFYRFYADEQQE

Sequences:

>Translated_652_residues
MPISEMLYRRPFRPVQGFLILLFSLTLFLTGFSLPSRAEITIGRSFDTAPIVLDGKVLFQVSKLREFSAKDRAKQANDSL
AQAIRDFAAAQDSPGVPTVKVTPGALPTLRLADRHLLTVTDRDVMLGQDAMEQAVGWQRTINRAIATGLKERTPSYRRQA
ILKSGLGVLLAILLHCGIQWIRLRWRRQQLRTMQDTKRPLALLGLTSLQLLVWVGVGYYICELFPQFRTLLYLVLRSFDE
PILILKEQEQGYGIVDLLIVVGLILGLWVIVRGLTLLLKSRILPLLGVNRGLQDAIAILTQYVLTGLGVVVILQVLGVDI
GALLIVGSALGLGIGFGLQNIVSNFISGIILLLDRPIEAGDFIKVGELVGSVEKIGTRSTELRTHDEVTIIVPNSHFLEK
EVVNWSHSSPVSRLHLPVDVAYGCDVDRVQTALIESVSHHPEVLAHPQPQVQFLGFGESALNFEVLVWFREPRQQYRLKS
DLYFRIEAMLRQYQIEIPFPQRDLHVRSPHLEQLIDLLVQQRTENAPHLYYPDSVRSQPPSQPILASPPPSPQPISRPSA
PQINLPELVAQMKGEQGLDIQDRRHRLNIYPQCFVGSEAVIWLMRTQRATRKEALRIGRLLMRKRIIHHVLDEHPFDDAY
LFYRFYADEQQE
>Mature_651_residues
PISEMLYRRPFRPVQGFLILLFSLTLFLTGFSLPSRAEITIGRSFDTAPIVLDGKVLFQVSKLREFSAKDRAKQANDSLA
QAIRDFAAAQDSPGVPTVKVTPGALPTLRLADRHLLTVTDRDVMLGQDAMEQAVGWQRTINRAIATGLKERTPSYRRQAI
LKSGLGVLLAILLHCGIQWIRLRWRRQQLRTMQDTKRPLALLGLTSLQLLVWVGVGYYICELFPQFRTLLYLVLRSFDEP
ILILKEQEQGYGIVDLLIVVGLILGLWVIVRGLTLLLKSRILPLLGVNRGLQDAIAILTQYVLTGLGVVVILQVLGVDIG
ALLIVGSALGLGIGFGLQNIVSNFISGIILLLDRPIEAGDFIKVGELVGSVEKIGTRSTELRTHDEVTIIVPNSHFLEKE
VVNWSHSSPVSRLHLPVDVAYGCDVDRVQTALIESVSHHPEVLAHPQPQVQFLGFGESALNFEVLVWFREPRQQYRLKSD
LYFRIEAMLRQYQIEIPFPQRDLHVRSPHLEQLIDLLVQQRTENAPHLYYPDSVRSQPPSQPILASPPPSPQPISRPSAP
QINLPELVAQMKGEQGLDIQDRRHRLNIYPQCFVGSEAVIWLMRTQRATRKEALRIGRLLMRKRIIHHVLDEHPFDDAYL
FYRFYADEQQE

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI189571664, Length=72, Percent_Identity=47.2222222222222, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI47578115, Length=84, Percent_Identity=39.2857142857143, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI47578117, Length=84, Percent_Identity=39.2857142857143, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1786670, Length=275, Percent_Identity=31.6363636363636, Blast_Score=177, Evalue=3e-45,
Organism=Escherichia coli, GI2367355, Length=244, Percent_Identity=32.7868852459016, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1789291, Length=250, Percent_Identity=28.4, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1787591, Length=197, Percent_Identity=28.9340101522843, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 73712; Mature: 73581

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS50186 DEP ; PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPISEMLYRRPFRPVQGFLILLFSLTLFLTGFSLPSRAEITIGRSFDTAPIVLDGKVLFQ
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEECCHHHHH
VSKLREFSAKDRAKQANDSLAQAIRDFAAAQDSPGVPTVKVTPGALPTLRLADRHLLTVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCCCEEEEEE
DRDVMLGQDAMEQAVGWQRTINRAIATGLKERTPSYRRQAILKSGLGVLLAILLHCGIQW
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHCCHHH
IRLRWRRQQLRTMQDTKRPLALLGLTSLQLLVWVGVGYYICELFPQFRTLLYLVLRSFDE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PILILKEQEQGYGIVDLLIVVGLILGLWVIVRGLTLLLKSRILPLLGVNRGLQDAIAILT
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QYVLTGLGVVVILQVLGVDIGALLIVGSALGLGIGFGLQNIVSNFISGIILLLDRPIEAG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DFIKVGELVGSVEKIGTRSTELRTHDEVTIIVPNSHFLEKEVVNWSHSSPVSRLHLPVDV
CCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCHHEECCEEE
AYGCDVDRVQTALIESVSHHPEVLAHPQPQVQFLGFGESALNFEVLVWFREPRQQYRLKS
ECCCCHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCCCEEEEEEECCHHHHHHHHH
DLYFRIEAMLRQYQIEIPFPQRDLHVRSPHLEQLIDLLVQQRTENAPHLYYPDSVRSQPP
HHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
SQPILASPPPSPQPISRPSAPQINLPELVAQMKGEQGLDIQDRRHRLNIYPQCFVGSEAV
CCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEECCHHH
IWLMRTQRATRKEALRIGRLLMRKRIIHHVLDEHPFDDAYLFYRFYADEQQE
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
PISEMLYRRPFRPVQGFLILLFSLTLFLTGFSLPSRAEITIGRSFDTAPIVLDGKVLFQ
CHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEECCHHHHH
VSKLREFSAKDRAKQANDSLAQAIRDFAAAQDSPGVPTVKVTPGALPTLRLADRHLLTVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCCCEEEEEE
DRDVMLGQDAMEQAVGWQRTINRAIATGLKERTPSYRRQAILKSGLGVLLAILLHCGIQW
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHCCHHH
IRLRWRRQQLRTMQDTKRPLALLGLTSLQLLVWVGVGYYICELFPQFRTLLYLVLRSFDE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PILILKEQEQGYGIVDLLIVVGLILGLWVIVRGLTLLLKSRILPLLGVNRGLQDAIAILT
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QYVLTGLGVVVILQVLGVDIGALLIVGSALGLGIGFGLQNIVSNFISGIILLLDRPIEAG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DFIKVGELVGSVEKIGTRSTELRTHDEVTIIVPNSHFLEKEVVNWSHSSPVSRLHLPVDV
CCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCHHEECCEEE
AYGCDVDRVQTALIESVSHHPEVLAHPQPQVQFLGFGESALNFEVLVWFREPRQQYRLKS
ECCCCHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCCCEEEEEEECCHHHHHHHHH
DLYFRIEAMLRQYQIEIPFPQRDLHVRSPHLEQLIDLLVQQRTENAPHLYYPDSVRSQPP
HHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
SQPILASPPPSPQPISRPSAPQINLPELVAQMKGEQGLDIQDRRHRLNIYPQCFVGSEAV
CCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEECCHHH
IWLMRTQRATRKEALRIGRLLMRKRIIHHVLDEHPFDDAYLFYRFYADEQQE
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]