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The map label for this gene is carA [H]

Identifier: 158338297

GI number: 158338297

Start: 5256932

End: 5258089

Strand: Direct

Name: carA [H]

Synonym: AM1_5193

Alternate gene names: 158338297

Gene position: 5256932-5258089 (Clockwise)

Preceding gene: 158338296

Following gene: 158338298

Centisome position: 80.83

GC content: 51.47

Gene sequence:

>1158_bases
ATGCTCAATGTTGATTCCCAACCCGCCCTCCTGGTTCTAGCTGATGGCAGTTCCTATCGCGGGTGGTCCTTTGGCGCACC
TGGCACTGCAGTGGGAGAAGTCGTCTTTAATACTGGCATGACCGGCTATCAAGAAGTGCTCACAGATCCCAGCTATTGTG
GACAGCTTGTCACCTTTACCTATCCTGAACTCGGCAACACGGGCGTCAATATTGAAGATGAAGAATCAGACCGTCCTCAA
GTCAAAGGGGCTATTGCCCGCAATCTTTGTCACCAACCCAGCAATTGGCGGTCCAACCACACCCTATCCAGCTACCTGCA
ACAACACCACATCCCTGGAATTTACGGCATTGATACACGAGCCCTAACTCGCAAAATCCGTTCCGCTGGTGCTATGAATG
GTGCCATATCCACCGAAATATTAGATTCGAACGCTTTGCTAGAAACAGTGTTGCAAGCCCCCTCCATGGAAGGGTTGAAT
TTGGCAGAGCGGGTCACTACCCATACCCCTTACGAATGGGTCGACCCAACCCCTGCAGCTTGGGAATTTAGCGAGTCCAC
TCCCCCTGCAGACTCAACTCAATCCCCTCTGACCGTCGTCGCCATCGACTTTGGGATCAAGCGAAATATCCTGCGACGCT
TAGCAAGCTATGGATGTCGAATCATTGTAGTTCCAGCTCATACCTCTGCCGCTGATATTCTCAGCCATCAGCCAGACGGT
ATTTTCCTATCCAACGGCCCTGGGGATCCAGCCGCTGTCACGGTAGGTATTGAAACAGCCAAAACCCTGATGGCCGAACA
AAAGCCCATGTTTGGCATCTGCTTGGGACACCAGTTATTGGGATTATCGCTGGGGGTCGATACATTCAAGCTCAAATTTG
GCCATCGAGGGCTGAATCAACCGGCAGGACTACAGCAGCAGGTCGAAATCACCAGTCAGAACCATGGCTTTGCCTTGAGT
GCTGAATCCATGACCCATGCCGAAGTAGAAGTCACCCATCTAAATCTCAACGATCAGACCATCGCTGGCCTAAAACACCA
ATCCTTACCGGTCTTCTCAGTGCAATATCACCCTGAAGCCAGCCCTGGCCCCCATGATGCGGATTATTTGTTTGAACGGT
TTGTGCAGTCCATGCTTGAGCATCGTTCAGGAAGCTAA

Upstream 100 bases:

>100_bases
TTGAAGGTGATTGAAGAGGCACTCCAGAAAGTGAGATTCTTTACTAAGGTTTACTATTAGCCTGCCTTTTAAACACTCGA
TATTCGCCCCATCCATCCCT

Downstream 100 bases:

>100_bases
GCCCCATTACGTACCGTGACCAAAGACCATTTATCTCCCCCTAGCTTAGCTGTTGAGATCTCTGGGGCAGGCTTCCCCAT
TCTCTGCTTGCACGGCCATC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MLNVDSQPALLVLADGSSYRGWSFGAPGTAVGEVVFNTGMTGYQEVLTDPSYCGQLVTFTYPELGNTGVNIEDEESDRPQ
VKGAIARNLCHQPSNWRSNHTLSSYLQQHHIPGIYGIDTRALTRKIRSAGAMNGAISTEILDSNALLETVLQAPSMEGLN
LAERVTTHTPYEWVDPTPAAWEFSESTPPADSTQSPLTVVAIDFGIKRNILRRLASYGCRIIVVPAHTSAADILSHQPDG
IFLSNGPGDPAAVTVGIETAKTLMAEQKPMFGICLGHQLLGLSLGVDTFKLKFGHRGLNQPAGLQQQVEITSQNHGFALS
AESMTHAEVEVTHLNLNDQTIAGLKHQSLPVFSVQYHPEASPGPHDADYLFERFVQSMLEHRSGS

Sequences:

>Translated_385_residues
MLNVDSQPALLVLADGSSYRGWSFGAPGTAVGEVVFNTGMTGYQEVLTDPSYCGQLVTFTYPELGNTGVNIEDEESDRPQ
VKGAIARNLCHQPSNWRSNHTLSSYLQQHHIPGIYGIDTRALTRKIRSAGAMNGAISTEILDSNALLETVLQAPSMEGLN
LAERVTTHTPYEWVDPTPAAWEFSESTPPADSTQSPLTVVAIDFGIKRNILRRLASYGCRIIVVPAHTSAADILSHQPDG
IFLSNGPGDPAAVTVGIETAKTLMAEQKPMFGICLGHQLLGLSLGVDTFKLKFGHRGLNQPAGLQQQVEITSQNHGFALS
AESMTHAEVEVTHLNLNDQTIAGLKHQSLPVFSVQYHPEASPGPHDADYLFERFVQSMLEHRSGS
>Mature_385_residues
MLNVDSQPALLVLADGSSYRGWSFGAPGTAVGEVVFNTGMTGYQEVLTDPSYCGQLVTFTYPELGNTGVNIEDEESDRPQ
VKGAIARNLCHQPSNWRSNHTLSSYLQQHHIPGIYGIDTRALTRKIRSAGAMNGAISTEILDSNALLETVLQAPSMEGLN
LAERVTTHTPYEWVDPTPAAWEFSESTPPADSTQSPLTVVAIDFGIKRNILRRLASYGCRIIVVPAHTSAADILSHQPDG
IFLSNGPGDPAAVTVGIETAKTLMAEQKPMFGICLGHQLLGLSLGVDTFKLKFGHRGLNQPAGLQQQVEITSQNHGFALS
AESMTHAEVEVTHLNLNDQTIAGLKHQSLPVFSVQYHPEASPGPHDADYLFERFVQSMLEHRSGS

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:
GO:0003824: Carbamoyl-phosphate synthase, small subunit
GO:0004086: Carbamoyl-phosphate synthase, small subunit
GO:0006221: Carbamoyl-phosphate synthase, small subunit
GO:0006541: Carbamoyl-phosphate synthase, small subunit
GO:0006807: Carbamoyl-phosphate synthase, small subunit
GO:0009058: Carbamoyl-phosphate synthase, small subunit

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=392, Percent_Identity=39.7959183673469, Blast_Score=258, Evalue=9e-69,
Organism=Homo sapiens, GI21361331, Length=390, Percent_Identity=35.6410256410256, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI169790915, Length=390, Percent_Identity=35.6410256410256, Blast_Score=237, Evalue=1e-62,
Organism=Escherichia coli, GI1786215, Length=381, Percent_Identity=49.3438320209974, Blast_Score=355, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI193204318, Length=393, Percent_Identity=38.676844783715, Blast_Score=244, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=403, Percent_Identity=37.9652605459057, Blast_Score=259, Evalue=7e-70,
Organism=Saccharomyces cerevisiae, GI6324878, Length=390, Percent_Identity=35.6410256410256, Blast_Score=224, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24642586, Length=394, Percent_Identity=36.8020304568528, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI45555749, Length=394, Percent_Identity=36.8020304568528, Blast_Score=228, Evalue=7e-60,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41680; Mature: 41680

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNVDSQPALLVLADGSSYRGWSFGAPGTAVGEVVFNTGMTGYQEVLTDPSYCGQLVTFT
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCHHHCCCEEEEE
YPELGNTGVNIEDEESDRPQVKGAIARNLCHQPSNWRSNHTLSSYLQQHHIPGIYGIDTR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEECCCHH
ALTRKIRSAGAMNGAISTEILDSNALLETVLQAPSMEGLNLAERVTTHTPYEWVDPTPAA
HHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCC
WEFSESTPPADSTQSPLTVVAIDFGIKRNILRRLASYGCRIIVVPAHTSAADILSHQPDG
EECCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCCCC
IFLSNGPGDPAAVTVGIETAKTLMAEQKPMFGICLGHQLLGLSLGVDTFKLKFGHRGLNQ
EEEECCCCCCEEEEEEHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCEEEEEECCCCCCC
PAGLQQQVEITSQNHGFALSAESMTHAEVEVTHLNLNDQTIAGLKHQSLPVFSVQYHPEA
CCCCCCEEEEECCCCCEEEECCCCCEEEEEEEEECCCCCHHHCCCCCCCCEEEEEECCCC
SPGPHDADYLFERFVQSMLEHRSGS
CCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNVDSQPALLVLADGSSYRGWSFGAPGTAVGEVVFNTGMTGYQEVLTDPSYCGQLVTFT
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCHHHCCCEEEEE
YPELGNTGVNIEDEESDRPQVKGAIARNLCHQPSNWRSNHTLSSYLQQHHIPGIYGIDTR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEECCCHH
ALTRKIRSAGAMNGAISTEILDSNALLETVLQAPSMEGLNLAERVTTHTPYEWVDPTPAA
HHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCC
WEFSESTPPADSTQSPLTVVAIDFGIKRNILRRLASYGCRIIVVPAHTSAADILSHQPDG
EECCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCCCC
IFLSNGPGDPAAVTVGIETAKTLMAEQKPMFGICLGHQLLGLSLGVDTFKLKFGHRGLNQ
EEEECCCCCCEEEEEEHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCEEEEEECCCCCCC
PAGLQQQVEITSQNHGFALSAESMTHAEVEVTHLNLNDQTIAGLKHQSLPVFSVQYHPEA
CCCCCCEEEEECCCCCEEEECCCCCEEEEEEEEECCCCCHHHCCCCCCCCEEEEEECCCC
SPGPHDADYLFERFVQSMLEHRSGS
CCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]