Click here to switch to the map view.

The map label for this gene is lacF [H]

Identifier: 158338294

GI number: 158338294

Start: 5254248

End: 5255174

Strand: Reverse

Name: lacF [H]

Synonym: AM1_5190

Alternate gene names: 158338294

Gene position: 5255174-5254248 (Counterclockwise)

Preceding gene: 158338295

Following gene: 158338293

Centisome position: 80.8

GC content: 48.65

Gene sequence:

>927_bases
ATGTCCCTCCGCAAATTGTCTTGGTCCACTGCGGCCCCGTATCTCTTTTTAGCCCCTGCCCTCTGTGTTCTCAGCTTGAG
TGTGTTTTGGCCAGCGCTACAAGCGTTTTATTTGAGTTTTACGCAGTTTGAGACCCTGGGCCAAACCCCAGAATGGATTG
GTCTGGGTAACTTTGAGCGGCTTTGGACGGACGATGTCTTTTGGCAAAGCTTGAAGAATTCGTTGATTTACCTGATTGGT
GTCGTTCCCATCTTGGTCATCCTTCCCTTGGGATTAGCGATCCTTGTTAACCAGAAGCTGAAAGGCATTCATTGGTTCCG
CGCCGCTTATTACACCCCGGTTGTGATTTCTATGGTGGTGGCGGGTATTGCCTGGAAGTGGCTATACGATGAGCGTGGTT
TATTAAATCAAGGCATTAGTTTGATTACCCAGAACCCACTAATGGAAACGATTCTCGGTCAGTTGGGCTGGACCGGTGAA
CCCATCGTTTGGCTGCAAAATCCAGATATCGCTCTTTACTGCGTAATGGCGGTTACGGTTTGGAAAGGGTTGGGATATTA
CATGGTGATTTATTTAGCGGGGTTACAAGCAATTCCCCCCGATCTCTATGAGGCCGCCGCAATTGATGGGGCAGATGGCT
GGCAAAAGCATTGGGATATCACCATTCCCTTGATGAAGCCTTATCTCATGCTAGTGTCCGTCATTTCAGCCATCTCAGCT
GCCAAGGTGTTTGAAGAAGTCTATGTCATGACCCCTGGCGGCGGGGTTCTGGGGAGTACGATTACGATTGTGGCTAATTT
ATATGAACAGGCTTTTTCTGAAGTGGGCAATGGCTATAGCTATGCCTGTGCGATGGGGCTCGTGTTATTCCTGCTGATCT
TTGGATTATCCATTTTGAATTTGAAGCTGGGGCAGCTCCGAGGGTAA

Upstream 100 bases:

>100_bases
TAACGCTGTTTCCGAGTAGATCGATATAGACTCAATATCGTTCCGTTCTCAGGGTTTTTGCTCTTCTCTTCAATTCAGTC
CTCTGCCAGTAAGAGACCCC

Downstream 100 bases:

>100_bases
TCCGTGATCTGAAGGAGAAATCTAATAAATATCCAACAAAACTAGACGATTTGATAAAAACGTAATAGAATCCAGCACGT
TCACAATTTGAGTGCAGATA

Product: sugar ABC transporter permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSLRKLSWSTAAPYLFLAPALCVLSLSVFWPALQAFYLSFTQFETLGQTPEWIGLGNFERLWTDDVFWQSLKNSLIYLIG
VVPILVILPLGLAILVNQKLKGIHWFRAAYYTPVVISMVVAGIAWKWLYDERGLLNQGISLITQNPLMETILGQLGWTGE
PIVWLQNPDIALYCVMAVTVWKGLGYYMVIYLAGLQAIPPDLYEAAAIDGADGWQKHWDITIPLMKPYLMLVSVISAISA
AKVFEEVYVMTPGGGVLGSTITIVANLYEQAFSEVGNGYSYACAMGLVLFLLIFGLSILNLKLGQLRG

Sequences:

>Translated_308_residues
MSLRKLSWSTAAPYLFLAPALCVLSLSVFWPALQAFYLSFTQFETLGQTPEWIGLGNFERLWTDDVFWQSLKNSLIYLIG
VVPILVILPLGLAILVNQKLKGIHWFRAAYYTPVVISMVVAGIAWKWLYDERGLLNQGISLITQNPLMETILGQLGWTGE
PIVWLQNPDIALYCVMAVTVWKGLGYYMVIYLAGLQAIPPDLYEAAAIDGADGWQKHWDITIPLMKPYLMLVSVISAISA
AKVFEEVYVMTPGGGVLGSTITIVANLYEQAFSEVGNGYSYACAMGLVLFLLIFGLSILNLKLGQLRG
>Mature_307_residues
SLRKLSWSTAAPYLFLAPALCVLSLSVFWPALQAFYLSFTQFETLGQTPEWIGLGNFERLWTDDVFWQSLKNSLIYLIGV
VPILVILPLGLAILVNQKLKGIHWFRAAYYTPVVISMVVAGIAWKWLYDERGLLNQGISLITQNPLMETILGQLGWTGEP
IVWLQNPDIALYCVMAVTVWKGLGYYMVIYLAGLQAIPPDLYEAAAIDGADGWQKHWDITIPLMKPYLMLVSVISAISAA
KVFEEVYVMTPGGGVLGSTITIVANLYEQAFSEVGNGYSYACAMGLVLFLLIFGLSILNLKLGQLRG

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789861, Length=308, Percent_Identity=29.2207792207792, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787570, Length=292, Percent_Identity=29.4520547945205, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790465, Length=268, Percent_Identity=29.1044776119403, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34157; Mature: 34026

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRKLSWSTAAPYLFLAPALCVLSLSVFWPALQAFYLSFTQFETLGQTPEWIGLGNFER
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
LWTDDVFWQSLKNSLIYLIGVVPILVILPLGLAILVNQKLKGIHWFRAAYYTPVVISMVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGIAWKWLYDERGLLNQGISLITQNPLMETILGQLGWTGEPIVWLQNPDIALYCVMAVTV
HHHHHHHHHCCCCHHHHCHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHH
WKGLGYYMVIYLAGLQAIPPDLYEAAAIDGADGWQKHWDITIPLMKPYLMLVSVISAISA
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
AKVFEEVYVMTPGGGVLGSTITIVANLYEQAFSEVGNGYSYACAMGLVLFLLIFGLSILN
HHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LKLGQLRG
HHHHHCCC
>Mature Secondary Structure 
SLRKLSWSTAAPYLFLAPALCVLSLSVFWPALQAFYLSFTQFETLGQTPEWIGLGNFER
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
LWTDDVFWQSLKNSLIYLIGVVPILVILPLGLAILVNQKLKGIHWFRAAYYTPVVISMVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGIAWKWLYDERGLLNQGISLITQNPLMETILGQLGWTGEPIVWLQNPDIALYCVMAVTV
HHHHHHHHHCCCCHHHHCHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHH
WKGLGYYMVIYLAGLQAIPPDLYEAAAIDGADGWQKHWDITIPLMKPYLMLVSVISAISA
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
AKVFEEVYVMTPGGGVLGSTITIVANLYEQAFSEVGNGYSYACAMGLVLFLLIFGLSILN
HHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LKLGQLRG
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]