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The map label for this gene is kinA [H]

Identifier: 158338214

GI number: 158338214

Start: 5169734

End: 5171371

Strand: Direct

Name: kinA [H]

Synonym: AM1_5108

Alternate gene names: 158338214

Gene position: 5169734-5171371 (Clockwise)

Preceding gene: 158338213

Following gene: 158338215

Centisome position: 79.49

GC content: 47.37

Gene sequence:

>1638_bases
ATGGAGAGTAGTACTGAAATTATGGTGGTGGATGATACCCCCGCCAATTTAGAGGTTATTTCTGAAACCTTGTCTTCTGC
AGGTTATCGAGTTGCCGCTGCTATTAGTGGGGAACGGGCTCTCAAACGCCTCCATACTCATCCTCCTGCCCTTATCCTTT
TGGATATCCAAATGCCAGTGATGGATGGGTTTGAAGTCTGTCGGCAAATCAAAGCCAATGCAGCAACGGCCAACATTCCC
ATCATCTTTATTACGGCTCGCTCAGATACCCAAAGCATTCTCCATGGCTTTGAGATGGGTGCCGTTGATTACATCAGCAA
ACCGTTTCAAGAAGCTGAACTCTTAGCTCGCGTTAGAACGCATTTGCAGCTTTGCCATGTCAGTCAACTCTATGATGTGG
AGCGCGAAAAAGCAGAGCAACTTCAGCAGCTCAATCAGCAACTGTCCCTCACCCAATTCTCGGTGGATCATTCCGTAGAT
GGCATTCTCTGGTTCAATCCAGATTCGAGTATCGCCTATGCCAATGGGGCAATCTGTGAATTGCTGGGATACGCCTTAGA
AGAGTTATTGCAACTATCCAGTGCGGATCTAAAAGCAACCGGCCATCATTCTGAATGGAACGACTTTCAACAAAAGGTTA
GAGAACAACACCGTTTAAGGTTTGAAGCTCAGTTACTTACTAAGGGGGGGCAAGAGCTGCCCGTGGAGATTGCCCTTAAC
TATCTTCAATTCTCTAGTCGTGAATATTATGTGGTGCATTGTCGCGATATTCGCGATCGCAAAGCAGCAGAAGCAAAGCT
GCGACAACTCAATACTGAACTAGACCAGCGGGTTCAAGACCGTACTCAAGAACTCTCGCACACCCTAGAAACCCTGAAAT
CAGCTCAAGCGAGTCTGGTCGAATCCGAAAAAATGGCTGCATTAGGGGCGCTTGTGGCCGGTGTCGCCCATGAAATAAAC
ACGCCGATTGGCAATACCATCACGGTAGCGTCCACCCTTGCCGATGAAAGTGCAGTCTTTGTGAATGCAGCAGAAGCAGG
ACAACTCAAGCGTTCAACCCTCAATCACTACCTTGATGTGGTCAATCGATGCACGAAGTTAATTAATAGCAATCTGGGCC
GAGCCGGAGACTTGATTCAAAGCTTCAAACAAGTTGCAGTGGATCAATCCCATCGCGAACTGCGGACGTTTAATCTGAAA
GACTATGTGGGAGAAGTGGTAACCAGCTTAGAACCCCAGGCAAGACGGTCAGGACTCACCTTAGACTTGGCAGGGGATGA
TTCCATTGTCCTCACTCATGATCCAGGGGTGTTTGCTCAAGTAATTACGAATCTAGTCACGAATTCCATCAAACATGCAT
ATCAGCCTGGAGAGGTTGGACAACTCCAGATTCACCTTGAACAGCAGAACGATCAGGTCCTGTTGACCTATCGTGATGAT
GGCTGTGGGATTTCTACTGAAAATTTGTCCAGGGTTTATGAACCCTTTTTTACGACTGCCCGGAATCAGGGGGGGACGGG
ATTAGGACTCAATATCGTCTACAACATTGTCACTCAATCTCTAAAAGGCACCATTAAGATTGATAGTCAGCTTGGACAAG
GAACAACCTTTACAGTTGCGCTTCCCTACGCATCCTAG

Upstream 100 bases:

>100_bases
TATCTCCCTTTCGCTGAACCTCTGCTGAAGTTAGCAAAACAGTATCGTACGGAAGAAATAGAAACTTTACTCCAGGAGTA
TTTGGCCCAGGAGCAAACAG

Downstream 100 bases:

>100_bases
ATTGCCTCACCCCAATGGTTAACGGGCTAAATCTGAGGCAAACTCTTTATGGCTGTGGTCCAATATCGAGTTTTGTAAAG
GTTACAGCGATCAAACGTTA

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Stage II sporulation protein F; Stage II sporulation protein J [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MESSTEIMVVDDTPANLEVISETLSSAGYRVAAAISGERALKRLHTHPPALILLDIQMPVMDGFEVCRQIKANAATANIP
IIFITARSDTQSILHGFEMGAVDYISKPFQEAELLARVRTHLQLCHVSQLYDVEREKAEQLQQLNQQLSLTQFSVDHSVD
GILWFNPDSSIAYANGAICELLGYALEELLQLSSADLKATGHHSEWNDFQQKVREQHRLRFEAQLLTKGGQELPVEIALN
YLQFSSREYYVVHCRDIRDRKAAEAKLRQLNTELDQRVQDRTQELSHTLETLKSAQASLVESEKMAALGALVAGVAHEIN
TPIGNTITVASTLADESAVFVNAAEAGQLKRSTLNHYLDVVNRCTKLINSNLGRAGDLIQSFKQVAVDQSHRELRTFNLK
DYVGEVVTSLEPQARRSGLTLDLAGDDSIVLTHDPGVFAQVITNLVTNSIKHAYQPGEVGQLQIHLEQQNDQVLLTYRDD
GCGISTENLSRVYEPFFTTARNQGGTGLGLNIVYNIVTQSLKGTIKIDSQLGQGTTFTVALPYAS

Sequences:

>Translated_545_residues
MESSTEIMVVDDTPANLEVISETLSSAGYRVAAAISGERALKRLHTHPPALILLDIQMPVMDGFEVCRQIKANAATANIP
IIFITARSDTQSILHGFEMGAVDYISKPFQEAELLARVRTHLQLCHVSQLYDVEREKAEQLQQLNQQLSLTQFSVDHSVD
GILWFNPDSSIAYANGAICELLGYALEELLQLSSADLKATGHHSEWNDFQQKVREQHRLRFEAQLLTKGGQELPVEIALN
YLQFSSREYYVVHCRDIRDRKAAEAKLRQLNTELDQRVQDRTQELSHTLETLKSAQASLVESEKMAALGALVAGVAHEIN
TPIGNTITVASTLADESAVFVNAAEAGQLKRSTLNHYLDVVNRCTKLINSNLGRAGDLIQSFKQVAVDQSHRELRTFNLK
DYVGEVVTSLEPQARRSGLTLDLAGDDSIVLTHDPGVFAQVITNLVTNSIKHAYQPGEVGQLQIHLEQQNDQVLLTYRDD
GCGISTENLSRVYEPFFTTARNQGGTGLGLNIVYNIVTQSLKGTIKIDSQLGQGTTFTVALPYAS
>Mature_545_residues
MESSTEIMVVDDTPANLEVISETLSSAGYRVAAAISGERALKRLHTHPPALILLDIQMPVMDGFEVCRQIKANAATANIP
IIFITARSDTQSILHGFEMGAVDYISKPFQEAELLARVRTHLQLCHVSQLYDVEREKAEQLQQLNQQLSLTQFSVDHSVD
GILWFNPDSSIAYANGAICELLGYALEELLQLSSADLKATGHHSEWNDFQQKVREQHRLRFEAQLLTKGGQELPVEIALN
YLQFSSREYYVVHCRDIRDRKAAEAKLRQLNTELDQRVQDRTQELSHTLETLKSAQASLVESEKMAALGALVAGVAHEIN
TPIGNTITVASTLADESAVFVNAAEAGQLKRSTLNHYLDVVNRCTKLINSNLGRAGDLIQSFKQVAVDQSHRELRTFNLK
DYVGEVVTSLEPQARRSGLTLDLAGDDSIVLTHDPGVFAQVITNLVTNSIKHAYQPGEVGQLQIHLEQQNDQVLLTYRDD
GCGISTENLSRVYEPFFTTARNQGGTGLGLNIVYNIVTQSLKGTIKIDSQLGQGTTFTVALPYAS

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=248, Percent_Identity=30.241935483871, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788394, Length=124, Percent_Identity=36.2903225806452, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI145693157, Length=265, Percent_Identity=26.0377358490566, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081816, Length=274, Percent_Identity=28.8321167883212, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87082012, Length=121, Percent_Identity=36.3636363636364, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790860, Length=155, Percent_Identity=32.258064516129, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=35.5932203389831, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=34.5132743362832, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1789149, Length=281, Percent_Identity=28.1138790035587, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1789809, Length=126, Percent_Identity=34.9206349206349, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786783, Length=235, Percent_Identity=28.5106382978723, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788549, Length=255, Percent_Identity=25.4901960784314, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786911, Length=119, Percent_Identity=35.2941176470588, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1790299, Length=120, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI48994928, Length=421, Percent_Identity=22.8028503562945, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790437, Length=122, Percent_Identity=34.4262295081967, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787229, Length=116, Percent_Identity=32.7586206896552, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788393, Length=129, Percent_Identity=36.4341085271318, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788550, Length=124, Percent_Identity=29.8387096774194, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI2367329, Length=178, Percent_Identity=33.1460674157303, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1788191, Length=110, Percent_Identity=30.9090909090909, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790863, Length=116, Percent_Identity=30.1724137931034, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=116, Percent_Identity=31.0344827586207, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1786912, Length=296, Percent_Identity=27.3648648648649, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR013655
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; PF08447 PAS_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60224; Mature: 60224

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESSTEIMVVDDTPANLEVISETLSSAGYRVAAAISGERALKRLHTHPPALILLDIQMPV
CCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEEEEEECCCH
MDGFEVCRQIKANAATANIPIIFITARSDTQSILHGFEMGAVDYISKPFQEAELLARVRT
HHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHH
HLQLCHVSQLYDVEREKAEQLQQLNQQLSLTQFSVDHSVDGILWFNPDSSIAYANGAICE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCEEECCCHHHH
LLGYALEELLQLSSADLKATGHHSEWNDFQQKVREQHRLRFEAQLLTKGGQELPVEIALN
HHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
YLQFSSREYYVVHCRDIRDRKAAEAKLRQLNTELDQRVQDRTQELSHTLETLKSAQASLV
HHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESEKMAALGALVAGVAHEINTPIGNTITVASTLADESAVFVNAAEAGQLKRSTLNHYLDV
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCCEEEEECHHHHHHHHHHHHHHHHH
VNRCTKLINSNLGRAGDLIQSFKQVAVDQSHRELRTFNLKDYVGEVVTSLEPQARRSGLT
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCHHHHCCCE
LDLAGDDSIVLTHDPGVFAQVITNLVTNSIKHAYQPGEVGQLQIHLEQQNDQVLLTYRDD
EEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEECC
GCGISTENLSRVYEPFFTTARNQGGTGLGLNIVYNIVTQSLKGTIKIDSQLGQGTTFTVA
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEE
LPYAS
ECCCC
>Mature Secondary Structure
MESSTEIMVVDDTPANLEVISETLSSAGYRVAAAISGERALKRLHTHPPALILLDIQMPV
CCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEEEEEECCCH
MDGFEVCRQIKANAATANIPIIFITARSDTQSILHGFEMGAVDYISKPFQEAELLARVRT
HHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHH
HLQLCHVSQLYDVEREKAEQLQQLNQQLSLTQFSVDHSVDGILWFNPDSSIAYANGAICE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCEEECCCHHHH
LLGYALEELLQLSSADLKATGHHSEWNDFQQKVREQHRLRFEAQLLTKGGQELPVEIALN
HHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
YLQFSSREYYVVHCRDIRDRKAAEAKLRQLNTELDQRVQDRTQELSHTLETLKSAQASLV
HHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESEKMAALGALVAGVAHEINTPIGNTITVASTLADESAVFVNAAEAGQLKRSTLNHYLDV
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCCEEEEECHHHHHHHHHHHHHHHHH
VNRCTKLINSNLGRAGDLIQSFKQVAVDQSHRELRTFNLKDYVGEVVTSLEPQARRSGLT
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCHHHHCCCE
LDLAGDDSIVLTHDPGVFAQVITNLVTNSIKHAYQPGEVGQLQIHLEQQNDQVLLTYRDD
EEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEECC
GCGISTENLSRVYEPFFTTARNQGGTGLGLNIVYNIVTQSLKGTIKIDSQLGQGTTFTVA
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEE
LPYAS
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2104615; 2509430; 9384377 [H]