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The map label for this gene is eda [H]

Identifier: 158338002

GI number: 158338002

Start: 4938357

End: 4939004

Strand: Reverse

Name: eda [H]

Synonym: AM1_4889

Alternate gene names: 158338002

Gene position: 4939004-4938357 (Counterclockwise)

Preceding gene: 158338003

Following gene: 158338001

Centisome position: 75.94

GC content: 51.54

Gene sequence:

>648_bases
TTGACGCAAAATCTTTGGTTAAAGACGGTGAGACATCATCGTGCAATTGGTGTAGTTCGGGCTCCTGAGTTAACCCTTGG
TGTTCGCATGGCGGAGGCGATTGCTCAAGCTGGGATGACGCTAATCGAAATTGCCTGGAATAGCTATCAATCCGCGACCC
TGGTGACTCAGCTCCGTGAAATTCTACCCAACTGTCAAATTGGTGTGGGAACGATTCTGACGGTTGATCAGCTACATTTT
GCGATCGCAGCGGGCGCACAGTTTTGTTTCTGCCCACATGTCAACTTGAACATTATTCAGGCAGCGGTGGCCCAAGATAT
CCCCATTATCCCTGGAGCCTTATCTCCCACTGAGATCGTTACGGCCTGGCAGGCAGGGGCCAGTAGTGTCAAGATTTTTC
CGGTGGAAATGATGGGAGGCAGCCAATATATCCAGAGTTTGCAGGGGCCTTTAGGGCATATCCCTGTGATTCCCACGGGG
GGCGTAACCCTAGAAAACGCGGTGGCCCTGATACAAGCAGGAGCAGTGGGTGTGGGGCTATCGAGTTACTTATTTCCCAA
AGAAGCACTCCAAGCTGAGGATTGGCCAGCTATCACCCGCCGAACAGAGCAGCTATTGCAACAATTACAGGACCTCTGCT
GCGATTAA

Upstream 100 bases:

>100_bases
CACGAGTGTTGAGATCGACCTTTAAGCATCTCTACATCCGCTGGATTAAAGAACCGATGGCAGCCTATGCTCAGCGGTAG
GTGACGATAGCTTTTTCACT

Downstream 100 bases:

>100_bases
TGGAGCGGCACAACTGGTCCAACAGCGCTCTCGTGAGGTTGGTTACAATGACGGGGAGCGATATGTTGGGAATGGATGAT
TATCGTGTTGATACTGGGGA

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MTQNLWLKTVRHHRAIGVVRAPELTLGVRMAEAIAQAGMTLIEIAWNSYQSATLVTQLREILPNCQIGVGTILTVDQLHF
AIAAGAQFCFCPHVNLNIIQAAVAQDIPIIPGALSPTEIVTAWQAGASSVKIFPVEMMGGSQYIQSLQGPLGHIPVIPTG
GVTLENAVALIQAGAVGVGLSSYLFPKEALQAEDWPAITRRTEQLLQQLQDLCCD

Sequences:

>Translated_215_residues
MTQNLWLKTVRHHRAIGVVRAPELTLGVRMAEAIAQAGMTLIEIAWNSYQSATLVTQLREILPNCQIGVGTILTVDQLHF
AIAAGAQFCFCPHVNLNIIQAAVAQDIPIIPGALSPTEIVTAWQAGASSVKIFPVEMMGGSQYIQSLQGPLGHIPVIPTG
GVTLENAVALIQAGAVGVGLSSYLFPKEALQAEDWPAITRRTEQLLQQLQDLCCD
>Mature_214_residues
TQNLWLKTVRHHRAIGVVRAPELTLGVRMAEAIAQAGMTLIEIAWNSYQSATLVTQLREILPNCQIGVGTILTVDQLHFA
IAAGAQFCFCPHVNLNIIQAAVAQDIPIIPGALSPTEIVTAWQAGASSVKIFPVEMMGGSQYIQSLQGPLGHIPVIPTGG
VTLENAVALIQAGAVGVGLSSYLFPKEALQAEDWPAITRRTEQLLQQLQDLCCD

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:
GO:0003824: 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase
GO:0008152: 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=180, Percent_Identity=28.8888888888889, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI48994954, Length=155, Percent_Identity=35.4838709677419, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.2.14 [H]

Molecular weight: Translated: 23043; Mature: 22912

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNLWLKTVRHHRAIGVVRAPELTLGVRMAEAIAQAGMTLIEIAWNSYQSATLVTQLRE
CCCCHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHH
ILPNCQIGVGTILTVDQLHFAIAAGAQFCFCPHVNLNIIQAAVAQDIPIIPGALSPTEIV
HCCCCCCCCCCEEHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHH
TAWQAGASSVKIFPVEMMGGSQYIQSLQGPLGHIPVIPTGGVTLENAVALIQAGAVGVGL
HHHHCCCCCEEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHCCH
SSYLFPKEALQAEDWPAITRRTEQLLQQLQDLCCD
HHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQNLWLKTVRHHRAIGVVRAPELTLGVRMAEAIAQAGMTLIEIAWNSYQSATLVTQLRE
CCCHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHH
ILPNCQIGVGTILTVDQLHFAIAAGAQFCFCPHVNLNIIQAAVAQDIPIIPGALSPTEIV
HCCCCCCCCCCEEHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHH
TAWQAGASSVKIFPVEMMGGSQYIQSLQGPLGHIPVIPTGGVTLENAVALIQAGAVGVGL
HHHHCCCCCEEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHCCH
SSYLFPKEALQAEDWPAITRRTEQLLQQLQDLCCD
HHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]