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The map label for this gene is rmlD [H]

Identifier: 158337932

GI number: 158337932

Start: 4854093

End: 4854992

Strand: Direct

Name: rmlD [H]

Synonym: AM1_4819

Alternate gene names: 158337932

Gene position: 4854093-4854992 (Clockwise)

Preceding gene: 158337931

Following gene: 158337935

Centisome position: 74.64

GC content: 51.22

Gene sequence:

>900_bases
ATGCTAAGTTCTAACGATTCCGCTCCAACCCCTAAGCTGTTAATAACGGGTGCCAGTGGCTTTTTGGGGTGGTATCTCTG
CCAGGTTGCCCAATCCAGCTGGCAGGTCTTTGGCACCGCTTATTCCCATGCCGTCGAGATCCCTGGATGCTCGGTAGTGA
CGGTTGATTTACGGGATTTTGCTAGCCTCAAACAGGTTTTGCAAACGGTTCAGCCGGATGCGGTCATTCACGCTGCTGCC
CAAGCACGTCCCCATGTCTGTCAAGCCGAGCCCCAATCGACCCATGCCATCAATGTAGAGGCGTCCTGGACCCTCGCTGA
CTTATGTGGGGAGTCTCAAATTCCATTGCTGTTTGTCTCAACCGATTTGGTTTTTGATGGGCAAGATCCTCCCTATCAGG
AATCTGATCTGGTTTCCCCCATCAATACCTATGGAGAACAGAAGGTAAAGGCAGAGCAAGGCATGGTGGAACGCTACCCT
TCCACAGTGATTGCTCGTATGCCTTTGATGTTTGGTGTGACGCCCCATGCAGCCAGTTTTATTCAACCGATGATCCAGAA
TTTAGAGGCAGGCATAGCGTTGCAGCTATTTGAGGATGAGTTTCGAACCCCTGTGAGTGGATTGGATGCGGCGCGTGGCA
TTCTGCTGGCGCTCGCCAAGGGGGAAGGATATATTCATCTAGGTGGCAAAGAACGCCTCTCCAGATTTGAAATGGGACAA
CAGTTAGTAGATTGCTTAGGATATCCGCCTACTCTATTAACCGGCTGTTCTCAGACAGATGTATCCCTGTCTACACCTCG
CCCTGCGGATGTCTCTTTGGATAGTTCTCTCGCCTATTCTCTGGGCTATGCCCCCAATCTTTTTGGCCAGGCGCTTCGTC
AGATGCTAGGTCAATCTTGA

Upstream 100 bases:

>100_bases
ATGGGCTTGCACCTCAGACTGAGGTTTTTCCTCTCGCTCAAGTCAATGAAGCGTTAGAGAAGTTACGCAGTCAGCACCCT
CCCTATCGTTTGGCCCTGAA

Downstream 100 bases:

>100_bases
ATTGGATTCAGGTTAACTACCCAACATAAAGGCAATCACAAATAAACCGTAAGTCAGTCCTAGTTTGAAGGTATTGAGAG
CTTCTCCCCAGAGGGCTTTG

Product: RmlD substrate binding domain-containing protein

Products: dTDP-L-rhamnose; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLSSNDSAPTPKLLITGASGFLGWYLCQVAQSSWQVFGTAYSHAVEIPGCSVVTVDLRDFASLKQVLQTVQPDAVIHAAA
QARPHVCQAEPQSTHAINVEASWTLADLCGESQIPLLFVSTDLVFDGQDPPYQESDLVSPINTYGEQKVKAEQGMVERYP
STVIARMPLMFGVTPHAASFIQPMIQNLEAGIALQLFEDEFRTPVSGLDAARGILLALAKGEGYIHLGGKERLSRFEMGQ
QLVDCLGYPPTLLTGCSQTDVSLSTPRPADVSLDSSLAYSLGYAPNLFGQALRQMLGQS

Sequences:

>Translated_299_residues
MLSSNDSAPTPKLLITGASGFLGWYLCQVAQSSWQVFGTAYSHAVEIPGCSVVTVDLRDFASLKQVLQTVQPDAVIHAAA
QARPHVCQAEPQSTHAINVEASWTLADLCGESQIPLLFVSTDLVFDGQDPPYQESDLVSPINTYGEQKVKAEQGMVERYP
STVIARMPLMFGVTPHAASFIQPMIQNLEAGIALQLFEDEFRTPVSGLDAARGILLALAKGEGYIHLGGKERLSRFEMGQ
QLVDCLGYPPTLLTGCSQTDVSLSTPRPADVSLDSSLAYSLGYAPNLFGQALRQMLGQS
>Mature_299_residues
MLSSNDSAPTPKLLITGASGFLGWYLCQVAQSSWQVFGTAYSHAVEIPGCSVVTVDLRDFASLKQVLQTVQPDAVIHAAA
QARPHVCQAEPQSTHAINVEASWTLADLCGESQIPLLFVSTDLVFDGQDPPYQESDLVSPINTYGEQKVKAEQGMVERYP
STVIARMPLMFGVTPHAASFIQPMIQNLEAGIALQLFEDEFRTPVSGLDAARGILLALAKGEGYIHLGGKERLSRFEMGQ
QLVDCLGYPPTLLTGCSQTDVSLSTPRPADVSLDSSLAYSLGYAPNLFGQALRQMLGQS

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=286, Percent_Identity=26.9230769230769, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI33519455, Length=286, Percent_Identity=26.9230769230769, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1788352, Length=227, Percent_Identity=25.1101321585903, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133 [C]

Molecular weight: Translated: 32191; Mature: 32191

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSSNDSAPTPKLLITGASGFLGWYLCQVAQSSWQVFGTAYSHAVEIPGCSVVTVDLRDF
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECHHHH
ASLKQVLQTVQPDAVIHAAAQARPHVCQAEPQSTHAINVEASWTLADLCGESQIPLLFVS
HHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCEEEEE
TDLVFDGQDPPYQESDLVSPINTYGEQKVKAEQGMVERYPSTVIARMPLMFGVTPHAASF
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHH
IQPMIQNLEAGIALQLFEDEFRTPVSGLDAARGILLALAKGEGYIHLGGKERLSRFEMGQ
HHHHHHHHCCCEEEEEEHHHHCCCHHHHHHHHCEEEEEECCCCEEEECCHHHHHHHHHHH
QLVDCLGYPPTLLTGCSQTDVSLSTPRPADVSLDSSLAYSLGYAPNLFGQALRQMLGQS
HHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSSNDSAPTPKLLITGASGFLGWYLCQVAQSSWQVFGTAYSHAVEIPGCSVVTVDLRDF
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECHHHH
ASLKQVLQTVQPDAVIHAAAQARPHVCQAEPQSTHAINVEASWTLADLCGESQIPLLFVS
HHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCEEEEE
TDLVFDGQDPPYQESDLVSPINTYGEQKVKAEQGMVERYPSTVIARMPLMFGVTPHAASF
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHH
IQPMIQNLEAGIALQLFEDEFRTPVSGLDAARGILLALAKGEGYIHLGGKERLSRFEMGQ
HHHHHHHHCCCEEEEEEHHHHCCCHHHHHHHHCEEEEEECCCCEEEECCHHHHHHHHHHH
QLVDCLGYPPTLLTGCSQTDVSLSTPRPADVSLDSSLAYSLGYAPNLFGQALRQMLGQS
HHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-L-mannose; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-L-mannose + H+ + Nicotinamide adenine dinucleotide phosphate - reduced --> dTDP-L-rhamnose + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]