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The map label for this gene is miaB

Identifier: 158337776

GI number: 158337776

Start: 4695150

End: 4696514

Strand: Direct

Name: miaB

Synonym: AM1_4660

Alternate gene names: 158337776

Gene position: 4695150-4696514 (Clockwise)

Preceding gene: 158337773

Following gene: 158337783

Centisome position: 72.19

GC content: 51.06

Gene sequence:

>1365_bases
ATGAGCTTAGATTCGCGCCGATACCACATCACTACCTATGGCTGTCAGATGAACAAAGCTGACTCTGAGCGGATGGCTGG
GGTTTTGGAAAACATGGGCTACCAATGGTCCGAGAACCCAGATGACGCCAATCTGATTCTCTGCAATACCTGCACGATTC
GAGACAATGCAGAGCATAAGGTCTATTCTTATCTGGGTCGGCAGGCGAAGCGCAAACATGCGCAACCAGATTTAACCCTA
GTTGTTGCGGGCTGTGTGGCCCAACAAGAAGGAGATGCCTTGCTGCGCCGGGTGCCCGAACTTGATTTGGTTATGGGACC
TCAACATGCCAATCGGTTGCAGGATTTATTAGAACAGGTGGCCAGCGGTCAACAGGTGTTGGCAACGGAGCCCATTCATA
TCGTTGAAGATATTACGAAGCCCCGTCGAGATAGTGCTGTCACTGCTTGGGTGAATGTGATTTATGGATGCAATGAACGC
TGCACCTATTGCGTCGTTCCTAATGTGCGGGGAGTCGAACAATCCCGTACGCCAGAGGCGATTCGAGCCGAAATGGTGCA
GCTCGGAGAACAAGGCTTCAAAGAGGTCACCCTCCTAGGACAGAATATCGACGCCTATGGTCGTGATCTACCCGGCACAA
CCTCAGAAGGGCGGCATCAGCATACCCTGACGGATCTCCTCTACTTTGTCCATGATGTGCCGGGTATTGAAAGAATTCGC
TTTGCCACCAGCCATCCTCGCTACTTTACTGAGCGTTTGATTCAGGCTTGCTATGAGTTGCCCAAGGTTTGCGAACATTT
CCACATTCCCTTTCAATCGGGGGATAACGATGTTCTCAAGGCCATGTCCCGAGGCTATACCCACGAGAAATACCGCCGCA
TCATTGATAATATTCGCGCGATCATGCCGGATGCCTCCATCAGTGCCGATGCAATTGTCGGCTTTCCGGGTGAAACTGAA
GAGCAGTTTATGAACACCATGCAGTTGGTCGAAGATATTGAATTCGATTTGCTGAATACAGCCGCTTATTCTCCTCGCCC
TGGGACACCTGCGGCCTTGTGGGATAACCAGCTTAGCGAAGAGGTTAAAGCCGATCGGCTGCAGCGATTAAATCGTTTAG
TTGGGGTGTGTGCGGAGTTGAGATCGCAACGTTATGCCAACCGCATCGAAGAAGTCCTCGTCGAAGACCAAAATCCCAAA
GATCCTACACAAGTCATGGGACGCACCCGCGGTAATCGTCTCACTTTCTTCCCTGGCAATATTGAGCAATTACGGGGAGA
AATTATCGCCGTCAAAGTCACAGAAGTGCGCTCTTTCAGTCTGACCGGAGAACCACTATCATCTGTGGCAACTGCCGTTC
GCTAG

Upstream 100 bases:

>100_bases
TTATAGACAGTGACTTTAGCGAACCCTTTCCTGAGTGAATTACCATAGGAACGGTCTCCCTTTTAACCTCATCCAGCCTC
TTACTTCGTTACATAACTTT

Downstream 100 bases:

>100_bases
GTGAAGTCTGTCCCCTAGATCATAGCATTGGTTTTAATGCGCTAAAAACTCCTCAACTTTTTTGGCACTCAAGATTTTTC
TGTGCTGCCTTAATGCTTCT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSLDSRRYHITTYGCQMNKADSERMAGVLENMGYQWSENPDDANLILCNTCTIRDNAEHKVYSYLGRQAKRKHAQPDLTL
VVAGCVAQQEGDALLRRVPELDLVMGPQHANRLQDLLEQVASGQQVLATEPIHIVEDITKPRRDSAVTAWVNVIYGCNER
CTYCVVPNVRGVEQSRTPEAIRAEMVQLGEQGFKEVTLLGQNIDAYGRDLPGTTSEGRHQHTLTDLLYFVHDVPGIERIR
FATSHPRYFTERLIQACYELPKVCEHFHIPFQSGDNDVLKAMSRGYTHEKYRRIIDNIRAIMPDASISADAIVGFPGETE
EQFMNTMQLVEDIEFDLLNTAAYSPRPGTPAALWDNQLSEEVKADRLQRLNRLVGVCAELRSQRYANRIEEVLVEDQNPK
DPTQVMGRTRGNRLTFFPGNIEQLRGEIIAVKVTEVRSFSLTGEPLSSVATAVR

Sequences:

>Translated_454_residues
MSLDSRRYHITTYGCQMNKADSERMAGVLENMGYQWSENPDDANLILCNTCTIRDNAEHKVYSYLGRQAKRKHAQPDLTL
VVAGCVAQQEGDALLRRVPELDLVMGPQHANRLQDLLEQVASGQQVLATEPIHIVEDITKPRRDSAVTAWVNVIYGCNER
CTYCVVPNVRGVEQSRTPEAIRAEMVQLGEQGFKEVTLLGQNIDAYGRDLPGTTSEGRHQHTLTDLLYFVHDVPGIERIR
FATSHPRYFTERLIQACYELPKVCEHFHIPFQSGDNDVLKAMSRGYTHEKYRRIIDNIRAIMPDASISADAIVGFPGETE
EQFMNTMQLVEDIEFDLLNTAAYSPRPGTPAALWDNQLSEEVKADRLQRLNRLVGVCAELRSQRYANRIEEVLVEDQNPK
DPTQVMGRTRGNRLTFFPGNIEQLRGEIIAVKVTEVRSFSLTGEPLSSVATAVR
>Mature_453_residues
SLDSRRYHITTYGCQMNKADSERMAGVLENMGYQWSENPDDANLILCNTCTIRDNAEHKVYSYLGRQAKRKHAQPDLTLV
VAGCVAQQEGDALLRRVPELDLVMGPQHANRLQDLLEQVASGQQVLATEPIHIVEDITKPRRDSAVTAWVNVIYGCNERC
TYCVVPNVRGVEQSRTPEAIRAEMVQLGEQGFKEVTLLGQNIDAYGRDLPGTTSEGRHQHTLTDLLYFVHDVPGIERIRF
ATSHPRYFTERLIQACYELPKVCEHFHIPFQSGDNDVLKAMSRGYTHEKYRRIIDNIRAIMPDASISADAIVGFPGETEE
QFMNTMQLVEDIEFDLLNTAAYSPRPGTPAALWDNQLSEEVKADRLQRLNRLVGVCAELRSQRYANRIEEVLVEDQNPKD
PTQVMGRTRGNRLTFFPGNIEQLRGEIIAVKVTEVRSFSLTGEPLSSVATAVR

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:
GO:0003824: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
GO:0005575: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
GO:0005737: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
GO:0006400: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
GO:0008033: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
GO:0009451: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
GO:0016740: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
GO:0043412: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
GO:0046872: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
GO:0051536: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
GO:0051539: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=33.5429769392034, Blast_Score=241, Evalue=8e-64,
Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=33.5429769392034, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI93277076, Length=456, Percent_Identity=29.1666666666667, Blast_Score=176, Evalue=4e-44,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=39.5505617977528, Blast_Score=321, Evalue=5e-89,
Organism=Escherichia coli, GI1787057, Length=446, Percent_Identity=26.0089686098655, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=459, Percent_Identity=33.1154684095861, Blast_Score=228, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI71996771, Length=386, Percent_Identity=28.2383419689119, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21356207, Length=485, Percent_Identity=30.7216494845361, Blast_Score=235, Evalue=6e-62,
Organism=Drosophila melanogaster, GI19922432, Length=461, Percent_Identity=28.8503253796095, Blast_Score=172, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_ACAM1 (B0C0E2)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518952.1
- ProteinModelPortal:   B0C0E2
- SMR:   B0C0E2
- GeneID:   5683462
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4660
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14333
- BioCyc:   AMAR329726:AM1_4660-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51261; Mature: 51129

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDSRRYHITTYGCQMNKADSERMAGVLENMGYQWSENPDDANLILCNTCTIRDNAEHK
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCHHH
VYSYLGRQAKRKHAQPDLTLVVAGCVAQQEGDALLRRVPELDLVMGPQHANRLQDLLEQV
HHHHHHHHHHHHCCCCCCEEEEEHHHHCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
ASGQQVLATEPIHIVEDITKPRRDSAVTAWVNVIYGCNERCTYCVVPNVRGVEQSRTPEA
HCCCEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHH
IRAEMVQLGEQGFKEVTLLGQNIDAYGRDLPGTTSEGRHQHTLTDLLYFVHDVPGIERIR
HHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHEE
FATSHPRYFTERLIQACYELPKVCEHFHIPFQSGDNDVLKAMSRGYTHEKYRRIIDNIRA
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
IMPDASISADAIVGFPGETEEQFMNTMQLVEDIEFDLLNTAAYSPRPGTPAALWDNQLSE
HCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
EVKADRLQRLNRLVGVCAELRSQRYANRIEEVLVEDQNPKDPTQVMGRTRGNRLTFFPGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEECCCC
IEQLRGEIIAVKVTEVRSFSLTGEPLSSVATAVR
HHHHCCCEEEEEEEEEEEEECCCCCHHHHHHHCC
>Mature Secondary Structure 
SLDSRRYHITTYGCQMNKADSERMAGVLENMGYQWSENPDDANLILCNTCTIRDNAEHK
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCHHH
VYSYLGRQAKRKHAQPDLTLVVAGCVAQQEGDALLRRVPELDLVMGPQHANRLQDLLEQV
HHHHHHHHHHHHCCCCCCEEEEEHHHHCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
ASGQQVLATEPIHIVEDITKPRRDSAVTAWVNVIYGCNERCTYCVVPNVRGVEQSRTPEA
HCCCEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHH
IRAEMVQLGEQGFKEVTLLGQNIDAYGRDLPGTTSEGRHQHTLTDLLYFVHDVPGIERIR
HHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHEE
FATSHPRYFTERLIQACYELPKVCEHFHIPFQSGDNDVLKAMSRGYTHEKYRRIIDNIRA
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
IMPDASISADAIVGFPGETEEQFMNTMQLVEDIEFDLLNTAAYSPRPGTPAALWDNQLSE
HCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
EVKADRLQRLNRLVGVCAELRSQRYANRIEEVLVEDQNPKDPTQVMGRTRGNRLTFFPGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEECCCC
IEQLRGEIIAVKVTEVRSFSLTGEPLSSVATAVR
HHHHCCCEEEEEEEEEEEEECCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA