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The map label for this gene is miaA

Identifier: 158337617

GI number: 158337617

Start: 4524970

End: 4525878

Strand: Reverse

Name: miaA

Synonym: AM1_4498

Alternate gene names: 158337617

Gene position: 4525878-4524970 (Counterclockwise)

Preceding gene: 158337620

Following gene: 158337616

Centisome position: 69.59

GC content: 50.94

Gene sequence:

>909_bases
ATGACGCCAGGGTTGATCGTAATTTGTGGGCCAACGGCTACCGGAAAATCCTCTCTGGCATTGGCTTTGGCCCGACGTTT
AGGTGCTCCCATTCTCAGTGCAGACTCTCGGCAAGTCTATCGGGGGTTTGATATTGGTACTGCGAAACCTTCTGTAACGG
ATCAAGAAGATGTTCCTCACTATCTCATTGATATATGTGACCCTACTGAGACTTTGACGGTTGCCGACTACCAAGAGCAG
GCTCAGGCGCTCATTGCTCAGTTTCATACTGAGGGGCAAACGCCGATTCTCGTGGGGGGGACTGGACTGTATATCCGGGC
GATTGTGGAGGGGCTGAAGATTCCGCGGGTACCGCCGCAACTGGAGTTGCGATCGCAACTCCAATCCCAAGGCCAGGTCC
AGATTTACCAATGGCTCCAGCAAGTCGATCCACCAGCAGCCCAAAAAATTCATGCCCATGACCAAGTCCGTACCCTAAGA
GCATTAGAAGTCTTTTACACCACGGGCATCCCCCTATCAGCACAGCAAGGCAAGAATCCCCCGTCCTATCCCATCTTGCA
AATTGGCTTAGATATTAGTGATCTGGACCAGCATACCGACATCATTCAGCAGCGCACGGCTGCCATGGTGGAGCAGGGTT
GGTTGACTGAAGTCCAAAAACTCATCGACCACTATGGGGTCGAACTCCCCCTCTTAGCAACCCTGGGCTATCAAGAAATG
AAGGCCTATCTGCATCAGCAAATCACCCTAGAAGAAGCAACCGCCCAAACTATTCTTCATACTCGTCAATTCGCCAAGCG
CCAACGCACCTGGTTTAGAGCGAATTCTGGCATTCACTGGTTTGATGCCACGAATTCTGACTTACTTTCTCTAGTTTGGA
AGGATATTCAAGGTCAACCTTGGCTGTAA

Upstream 100 bases:

>100_bases
CCCCAAATTCTATCACAAATGCCTTTTAAGCGATTTTAGACCTGTCTTAAGGGCTGTTTACCGTGGGGTTGCATTATTAA
TTCTTTAGGATTCAGTAGGA

Downstream 100 bases:

>100_bases
AGAATAAACTCCATCCATCACCCCCAAAATCTGACTGACCTTTGTTAAAATAGCTGGGTTGTTTCGGCACAGTACCTCTT
TGAGCCAGTCTCTCGATTTA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTPGLIVICGPTATGKSSLALALARRLGAPILSADSRQVYRGFDIGTAKPSVTDQEDVPHYLIDICDPTETLTVADYQEQ
AQALIAQFHTEGQTPILVGGTGLYIRAIVEGLKIPRVPPQLELRSQLQSQGQVQIYQWLQQVDPPAAQKIHAHDQVRTLR
ALEVFYTTGIPLSAQQGKNPPSYPILQIGLDISDLDQHTDIIQQRTAAMVEQGWLTEVQKLIDHYGVELPLLATLGYQEM
KAYLHQQITLEEATAQTILHTRQFAKRQRTWFRANSGIHWFDATNSDLLSLVWKDIQGQPWL

Sequences:

>Translated_302_residues
MTPGLIVICGPTATGKSSLALALARRLGAPILSADSRQVYRGFDIGTAKPSVTDQEDVPHYLIDICDPTETLTVADYQEQ
AQALIAQFHTEGQTPILVGGTGLYIRAIVEGLKIPRVPPQLELRSQLQSQGQVQIYQWLQQVDPPAAQKIHAHDQVRTLR
ALEVFYTTGIPLSAQQGKNPPSYPILQIGLDISDLDQHTDIIQQRTAAMVEQGWLTEVQKLIDHYGVELPLLATLGYQEM
KAYLHQQITLEEATAQTILHTRQFAKRQRTWFRANSGIHWFDATNSDLLSLVWKDIQGQPWL
>Mature_301_residues
TPGLIVICGPTATGKSSLALALARRLGAPILSADSRQVYRGFDIGTAKPSVTDQEDVPHYLIDICDPTETLTVADYQEQA
QALIAQFHTEGQTPILVGGTGLYIRAIVEGLKIPRVPPQLELRSQLQSQGQVQIYQWLQQVDPPAAQKIHAHDQVRTLRA
LEVFYTTGIPLSAQQGKNPPSYPILQIGLDISDLDQHTDIIQQRTAAMVEQGWLTEVQKLIDHYGVELPLLATLGYQEMK
AYLHQQITLEEATAQTILHTRQFAKRQRTWFRANSGIHWFDATNSDLLSLVWKDIQGQPWL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:
GO:0000166: tRNA dimethylallyltransferase
GO:0005524: tRNA dimethylallyltransferase
GO:0008033: tRNA dimethylallyltransferase
GO:0016740: tRNA dimethylallyltransferase
GO:0052381: tRNA dimethylallyltransferase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=315, Percent_Identity=31.4285714285714, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1790613, Length=292, Percent_Identity=36.986301369863, Blast_Score=191, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI25144712, Length=186, Percent_Identity=26.8817204301075, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324848, Length=309, Percent_Identity=27.831715210356, Blast_Score=119, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24649830, Length=265, Percent_Identity=31.6981132075472, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_ACAM1 (B0CG28)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518792.1
- ProteinModelPortal:   B0CG28
- SMR:   B0CG28
- GeneID:   5683300
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4498
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- BioCyc:   AMAR329726:AM1_4498-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33717; Mature: 33586

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPGLIVICGPTATGKSSLALALARRLGAPILSADSRQVYRGFDIGTAKPSVTDQEDVPH
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCH
YLIDICDPTETLTVADYQEQAQALIAQFHTEGQTPILVGGTGLYIRAIVEGLKIPRVPPQ
HEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCCH
LELRSQLQSQGQVQIYQWLQQVDPPAAQKIHAHDQVRTLRALEVFYTTGIPLSAQQGKNP
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCC
PSYPILQIGLDISDLDQHTDIIQQRTAAMVEQGWLTEVQKLIDHYGVELPLLATLGYQEM
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHH
KAYLHQQITLEEATAQTILHTRQFAKRQRTWFRANSGIHWFDATNSDLLSLVWKDIQGQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCC
WL
CC
>Mature Secondary Structure 
TPGLIVICGPTATGKSSLALALARRLGAPILSADSRQVYRGFDIGTAKPSVTDQEDVPH
CCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCH
YLIDICDPTETLTVADYQEQAQALIAQFHTEGQTPILVGGTGLYIRAIVEGLKIPRVPPQ
HEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCCH
LELRSQLQSQGQVQIYQWLQQVDPPAAQKIHAHDQVRTLRALEVFYTTGIPLSAQQGKNP
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCC
PSYPILQIGLDISDLDQHTDIIQQRTAAMVEQGWLTEVQKLIDHYGVELPLLATLGYQEM
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHH
KAYLHQQITLEEATAQTILHTRQFAKRQRTWFRANSGIHWFDATNSDLLSLVWKDIQGQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCC
WL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA