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The map label for this gene is purN [H]

Identifier: 158337478

GI number: 158337478

Start: 4386269

End: 4386940

Strand: Reverse

Name: purN [H]

Synonym: AM1_4357

Alternate gene names: 158337478

Gene position: 4386940-4386269 (Counterclockwise)

Preceding gene: 158337479

Following gene: 158337477

Centisome position: 67.45

GC content: 51.49

Gene sequence:

>672_bases
ATGAGTTTTTCTGCTTCAGAATCTGTGTCTCCAGCTCTGGTCTCTCCTGATGAGATATCCCTAGAGAAGTTATCTTCGTC
TCCCCCTCTAAAACTGGGGATTATGGCCTCGGGAACCGGGAGTAACTTTGTTGCGATCGCAGATGCCATTGCCCAACATC
ATCTGGCCGCCCACATCCAGGTGGTTATTTATAACAATCCCGATGCCCCCGTTGCCCAGCGGGCCCAGGAACGTCAGATC
CCCACCCACCTCATCAATCATCGCCATTTTTCAACGCGGGAGGTCTTTGATCAGCAGATCGTTGACCGTTTGCGAGAGGC
AGACGTGGACTGGGTCGTTATGGTGGGATGGATGCGTCGAGTCACTCAAGTTCTGATTGACGCTTTCCCGGATCGCATGA
TCAATATTCACCCCAGTCTTTTACCTAGTTTTCCAGGGATACGAGCCATTGAGCAGGCCCTTGAGCATCAGGTCAAAATT
TCAGGCTGCACGGTGCATATCGTTCGCTTAGAAGTTGACAGCGGCCCCATTTTGATTCAAGCGGCAGTTCCGGTTTATCC
CGAAGACACCCCAGCCAGCTTACATCGACGCATTCAAATTCAAGAACACCGCATTATTGTCCAAGCCATTGCCCAGCTGA
TTCAGAACCGTCTCACCCCTTGCCGCCAATAA

Upstream 100 bases:

>100_bases
ACCAAAACTTGATTCTGTTGTAAGTTCTGATTTTGCCTAAGATCCCTGGGGTATTCCCGCAGTATTTTAGACAGGCACTG
ACCCTGGCTTCTCCAACCCT

Downstream 100 bases:

>100_bases
AAAACTAAGCAGATGCAAGATCTCTCATCTATGATTAGGCTTATTTCTTTCTTGAGAAAATAAATAAAGAGCTGATTCTG
TCGTTATGTCTCACTATATG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSFSASESVSPALVSPDEISLEKLSSSPPLKLGIMASGTGSNFVAIADAIAQHHLAAHIQVVIYNNPDAPVAQRAQERQI
PTHLINHRHFSTREVFDQQIVDRLREADVDWVVMVGWMRRVTQVLIDAFPDRMINIHPSLLPSFPGIRAIEQALEHQVKI
SGCTVHIVRLEVDSGPILIQAAVPVYPEDTPASLHRRIQIQEHRIIVQAIAQLIQNRLTPCRQ

Sequences:

>Translated_223_residues
MSFSASESVSPALVSPDEISLEKLSSSPPLKLGIMASGTGSNFVAIADAIAQHHLAAHIQVVIYNNPDAPVAQRAQERQI
PTHLINHRHFSTREVFDQQIVDRLREADVDWVVMVGWMRRVTQVLIDAFPDRMINIHPSLLPSFPGIRAIEQALEHQVKI
SGCTVHIVRLEVDSGPILIQAAVPVYPEDTPASLHRRIQIQEHRIIVQAIAQLIQNRLTPCRQ
>Mature_222_residues
SFSASESVSPALVSPDEISLEKLSSSPPLKLGIMASGTGSNFVAIADAIAQHHLAAHIQVVIYNNPDAPVAQRAQERQIP
THLINHRHFSTREVFDQQIVDRLREADVDWVVMVGWMRRVTQVLIDAFPDRMINIHPSLLPSFPGIRAIEQALEHQVKIS
GCTVHIVRLEVDSGPILIQAAVPVYPEDTPASLHRRIQIQEHRIIVQAIAQLIQNRLTPCRQ

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:
GO:0004644: Phosphoribosylglycinamide formyltransferase
GO:0006189: Phosphoribosylglycinamide formyltransferase
GO:0009058: Phosphoribosylglycinamide formyltransferase
GO:0016740: Phosphoribosylglycinamide formyltransferase
GO:0016742: Phosphoribosylglycinamide formyltransferase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=184, Percent_Identity=41.304347826087, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI209869995, Length=184, Percent_Identity=41.304347826087, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI209869993, Length=184, Percent_Identity=41.304347826087, Blast_Score=145, Evalue=3e-35,
Organism=Escherichia coli, GI1788846, Length=185, Percent_Identity=35.6756756756757, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1787483, Length=139, Percent_Identity=34.5323741007194, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=33.7016574585635, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320616, Length=205, Percent_Identity=28.2926829268293, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582400, Length=197, Percent_Identity=39.0862944162437, Blast_Score=147, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 24846; Mature: 24715

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSASESVSPALVSPDEISLEKLSSSPPLKLGIMASGTGSNFVAIADAIAQHHLAAHIQ
CCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHEEEE
VVIYNNPDAPVAQRAQERQIPTHLINHRHFSTREVFDQQIVDRLREADVDWVVMVGWMRR
EEEECCCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VTQVLIDAFPDRMINIHPSLLPSFPGIRAIEQALEHQVKISGCTVHIVRLEVDSGPILIQ
HHHHHHHHCCCCEEECCHHHCCCCCCHHHHHHHHHHCEEECCEEEEEEEEEECCCCEEEE
AAVPVYPEDTPASLHRRIQIQEHRIIVQAIAQLIQNRLTPCRQ
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SFSASESVSPALVSPDEISLEKLSSSPPLKLGIMASGTGSNFVAIADAIAQHHLAAHIQ
CCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHEEEE
VVIYNNPDAPVAQRAQERQIPTHLINHRHFSTREVFDQQIVDRLREADVDWVVMVGWMRR
EEEECCCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VTQVLIDAFPDRMINIHPSLLPSFPGIRAIEQALEHQVKISGCTVHIVRLEVDSGPILIQ
HHHHHHHHCCCCEEECCHHHCCCCCCHHHHHHHHHHCEEECCEEEEEEEEEECCCCEEEE
AAVPVYPEDTPASLHRRIQIQEHRIIVQAIAQLIQNRLTPCRQ
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]