Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 158337392

GI number: 158337392

Start: 4289519

End: 4290955

Strand: Reverse

Name: braE [H]

Synonym: AM1_4271

Alternate gene names: 158337392

Gene position: 4290955-4289519 (Counterclockwise)

Preceding gene: 158337395

Following gene: 158337391

Centisome position: 65.98

GC content: 52.26

Gene sequence:

>1437_bases
ATGGAAGTTGGCTATTTTGTTTCTCTGGCGCTGTTCACCACCATTTATGCTCTATTCAGTCTAGGACTGAATTTACAGTG
GGGTTATACCGGGCTCATTAACTTTGGTCATATTGCCTTTATGGCGATTGGAGCCTACACCACCATTGCCTTGACCCTAC
CCAACTTCATCAAAGAAATGCCCCAGCGGCTGATGGAGCAATATCCAGCGATTGCCAACCAAGAGGGGCTGGCCTCTATG
TTGAACTTTCTAGGGGATGTCCTGCCGGAACAGGTTCCTTTAGTGATTGCCGTGCTGACCGGGGCTTTGTTAGCGGCACT
ACTGGGATTAGCCATCGGCACCAGTACGCTACGACTCCGGGAAGATTACCTGTCCATTGTCACCATTGGCGTCTCGGAGG
TTGTTCGCTTGGTGGCCGTCAATGAAGAATGGCTGACCCGAGGACCGCGAGGCGTCTTTAACTATCCCCTACCCTTAGAG
AAACTAGAGCCTAATCTGGTGATTCAAGGAGGGATGATTGCCTCCTTAGTCTTGGTCTTGGGGCTGGCCCTGTGGCGACT
ATGGCAATGGCTTCTACAGCAGCCTCAACGGGATGGTCGTCCCCCCTTGTCTCCCCCGCTATTTGTCGGCTATGTCCTCT
GCTTAGTTGGCGTTGCCGCCTGCTGGTGGTTTTCCTTTTCCCTCAGCCTCACCATTGAGCTGCCGTCAGCGTTACAGGAA
ATCGTCTTGCTGATGATGCGGTTCGCCATGGTCGCGCCCCTAGGAATTGTGGGGGTTATTCTGGTCTATGCGTCCTGTCG
GCAACCCTTGCAGCAACTCCGCACCCAGTCCATGGGGACCCACCTCAGCCTACTCGCCGTTTCCATGGGAATGGGATTAG
TGGTGATGTTTTTTGGGGTTCACATCATGGGCATTATCGACTACACCTACAAAGCAGGACTGCTATGGCTGCTGATTTTG
ACCATTGCCCTGGTCTTTTGGCAGTTGGAGCGCTGGGTCAATTCACCGTGGGGTCGAGTATTAACCTCGATCCGAGAAGA
TGAGGAAGTCGCCAAAGCCCTGGGTAAAAATGTCTTTCGCTACAAGCTGCAGTCCTTTATGTTAGGCGGTGCGATCGCAG
GTATTGCTGGATCGTTTTATGCCTGGCAGCTCACCTTTATTAACCCCGATGGTTTTATTCCCCTCCTCACCTTCCAAGCC
TGGATTATTATTGTGGCTGGAGGCGCAGGCAATAACGTGGGCACCCTGCTGGGAGCTGGAATTTTCTGGGCCTACAATGA
GGTGACTCGGTTTATCTTGCCCAATATCCTGCCCCTAGATGATGCGCGATTGGGGGCTTTTCGGGTCATGGTGATTGGCC
TAATGTTAATTGTTTTGATGATGTGGCGACCCCAAGGTCTGCTGGGTAAAAAGGAGGAACTCACCCTTGGAAGATAA

Upstream 100 bases:

>100_bases
CTTTAGCATTTCTGAGCAGAGGAAATATCCCCTAGCCTGATAAAATCCTGAAGACGTTGATTTAGGCGGCGCACCCGCGC
TTTTACCCCCTCCCAAGCCT

Downstream 100 bases:

>100_bases
GCGCCAGCCCGCCCCTCCAGTCCAACCAGAAAAAATGCTGTTGACCGCCCAGGGACTCTCCAAAGCCTTTGGCGGTATCC
AAGCTGTGAATAATGCTGAA

Product: branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MEVGYFVSLALFTTIYALFSLGLNLQWGYTGLINFGHIAFMAIGAYTTIALTLPNFIKEMPQRLMEQYPAIANQEGLASM
LNFLGDVLPEQVPLVIAVLTGALLAALLGLAIGTSTLRLREDYLSIVTIGVSEVVRLVAVNEEWLTRGPRGVFNYPLPLE
KLEPNLVIQGGMIASLVLVLGLALWRLWQWLLQQPQRDGRPPLSPPLFVGYVLCLVGVAACWWFSFSLSLTIELPSALQE
IVLLMMRFAMVAPLGIVGVILVYASCRQPLQQLRTQSMGTHLSLLAVSMGMGLVVMFFGVHIMGIIDYTYKAGLLWLLIL
TIALVFWQLERWVNSPWGRVLTSIREDEEVAKALGKNVFRYKLQSFMLGGAIAGIAGSFYAWQLTFINPDGFIPLLTFQA
WIIIVAGGAGNNVGTLLGAGIFWAYNEVTRFILPNILPLDDARLGAFRVMVIGLMLIVLMMWRPQGLLGKKEELTLGR

Sequences:

>Translated_478_residues
MEVGYFVSLALFTTIYALFSLGLNLQWGYTGLINFGHIAFMAIGAYTTIALTLPNFIKEMPQRLMEQYPAIANQEGLASM
LNFLGDVLPEQVPLVIAVLTGALLAALLGLAIGTSTLRLREDYLSIVTIGVSEVVRLVAVNEEWLTRGPRGVFNYPLPLE
KLEPNLVIQGGMIASLVLVLGLALWRLWQWLLQQPQRDGRPPLSPPLFVGYVLCLVGVAACWWFSFSLSLTIELPSALQE
IVLLMMRFAMVAPLGIVGVILVYASCRQPLQQLRTQSMGTHLSLLAVSMGMGLVVMFFGVHIMGIIDYTYKAGLLWLLIL
TIALVFWQLERWVNSPWGRVLTSIREDEEVAKALGKNVFRYKLQSFMLGGAIAGIAGSFYAWQLTFINPDGFIPLLTFQA
WIIIVAGGAGNNVGTLLGAGIFWAYNEVTRFILPNILPLDDARLGAFRVMVIGLMLIVLMMWRPQGLLGKKEELTLGR
>Mature_478_residues
MEVGYFVSLALFTTIYALFSLGLNLQWGYTGLINFGHIAFMAIGAYTTIALTLPNFIKEMPQRLMEQYPAIANQEGLASM
LNFLGDVLPEQVPLVIAVLTGALLAALLGLAIGTSTLRLREDYLSIVTIGVSEVVRLVAVNEEWLTRGPRGVFNYPLPLE
KLEPNLVIQGGMIASLVLVLGLALWRLWQWLLQQPQRDGRPPLSPPLFVGYVLCLVGVAACWWFSFSLSLTIELPSALQE
IVLLMMRFAMVAPLGIVGVILVYASCRQPLQQLRTQSMGTHLSLLAVSMGMGLVVMFFGVHIMGIIDYTYKAGLLWLLIL
TIALVFWQLERWVNSPWGRVLTSIREDEEVAKALGKNVFRYKLQSFMLGGAIAGIAGSFYAWQLTFINPDGFIPLLTFQA
WIIIVAGGAGNNVGTLLGAGIFWAYNEVTRFILPNILPLDDARLGAFRVMVIGLMLIVLMMWRPQGLLGKKEELTLGR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=140, Percent_Identity=29.2857142857143, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 52839; Mature: 52839

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVGYFVSLALFTTIYALFSLGLNLQWGYTGLINFGHIAFMAIGAYTTIALTLPNFIKEM
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PQRLMEQYPAIANQEGLASMLNFLGDVLPEQVPLVIAVLTGALLAALLGLAIGTSTLRLR
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
EDYLSIVTIGVSEVVRLVAVNEEWLTRGPRGVFNYPLPLEKLEPNLVIQGGMIASLVLVL
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHCCCCEEEECCHHHHHHHHH
GLALWRLWQWLLQQPQRDGRPPLSPPLFVGYVLCLVGVAACWWFSFSLSLTIELPSALQE
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHH
IVLLMMRFAMVAPLGIVGVILVYASCRQPLQQLRTQSMGTHLSLLAVSMGMGLVVMFFGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
HIMGIIDYTYKAGLLWLLILTIALVFWQLERWVNSPWGRVLTSIREDEEVAKALGKNVFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
YKLQSFMLGGAIAGIAGSFYAWQLTFINPDGFIPLLTFQAWIIIVAGGAGNNVGTLLGAG
HHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHEEEEECCCCCCHHHHHHHH
IFWAYNEVTRFILPNILPLDDARLGAFRVMVIGLMLIVLMMWRPQGLLGKKEELTLGR
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEVGYFVSLALFTTIYALFSLGLNLQWGYTGLINFGHIAFMAIGAYTTIALTLPNFIKEM
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PQRLMEQYPAIANQEGLASMLNFLGDVLPEQVPLVIAVLTGALLAALLGLAIGTSTLRLR
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
EDYLSIVTIGVSEVVRLVAVNEEWLTRGPRGVFNYPLPLEKLEPNLVIQGGMIASLVLVL
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHCCCCEEEECCHHHHHHHHH
GLALWRLWQWLLQQPQRDGRPPLSPPLFVGYVLCLVGVAACWWFSFSLSLTIELPSALQE
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHH
IVLLMMRFAMVAPLGIVGVILVYASCRQPLQQLRTQSMGTHLSLLAVSMGMGLVVMFFGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
HIMGIIDYTYKAGLLWLLILTIALVFWQLERWVNSPWGRVLTSIREDEEVAKALGKNVFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
YKLQSFMLGGAIAGIAGSFYAWQLTFINPDGFIPLLTFQAWIIIVAGGAGNNVGTLLGAG
HHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHEEEEECCCCCCHHHHHHHH
IFWAYNEVTRFILPNILPLDDARLGAFRVMVIGLMLIVLMMWRPQGLLGKKEELTLGR
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]