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The map label for this gene is argG

Identifier: 158337356

GI number: 158337356

Start: 4253294

End: 4254493

Strand: Direct

Name: argG

Synonym: AM1_4234

Alternate gene names: 158337356

Gene position: 4253294-4254493 (Clockwise)

Preceding gene: 158337353

Following gene: 158337358

Centisome position: 65.4

GC content: 51.42

Gene sequence:

>1200_bases
ATGGGGCGCGCTAAAAAAGTTGTGTTGGCCTATTCTGGAGGCGTTGACACCTCCGTTTGTATTCCGTATCTCAAGCACGA
ATGGGGTGTTGAAGAAGTTATCACCCTGGCTGCTGACTTAGGCCAAGGGGATGAGCTAGAGCCCATTCGCAAAAAAGCCC
TAGATGCAGGAGCTGCCCAATCTTTAATTGCCGATGGCACCGAAAGCTTTATTGCTGACTATGCCTTTCCTGCCATCCAA
GCCAACGCCCTCTACGAAAATCGATATCCCTTATCGACGGCCTTAGCTCGACCATTAATTGCCAAATTGCTGGTGGAAGC
CGCTGAGATCCATGGAGCCGATGCTGTTGCCCATGGCTGCACGGGTAAAGGCAATGATCAGGTGCGGTTTGATGTGTCTA
TTACCGCTCTTAATCCCGACATCAAAGTGCTAGCTCCTGCCCGTGAATGGGGTATGAGCCGGGAAGAAACCATTGCCTAT
GGCGAAAAGTATGGCGTGCAGTCCCCCGTCAAAAAATCTTCCCCCTACAGTATTGACCGCAATTTATTAGGGCGGAGTGT
GGAAGCTGGCCCCTTGGAAGATCCTTGGGTCGAACCGTTAGAGGAAGTCTACGATCTAACGAAGGCCATTGCCGATACCC
CTGATCAGCCCGAGTACGTTGATATTGATTTTGCCCAGGGTCTTCCTACGCAGCTAAATGGCCAGGCCCTAAGCCCTGTT
GCTCTAGTCGAACAGCTCAATCAGATCGTCGGCAATCATGGGGTGGGCCGTATCGATATGGTGGAAAATCGCCTGGTCGG
TATTAAATCTCGGGAAATCTATGAAGCCCCTGCTCTGCTTGTACTGATTCAAGCTCATCGGGAACTAGAGAGCCTTGCCC
TGACCGCAGATGTTACCCATTACAAGCGAGGCATTGAAGAAACCTATAGCCAACTCGTCTATAACGGCCTCTGGTTTAGC
CCTCTCAAAGATGCCCTAGACGCCTTTATTCAGCAAACACAAAAACAGGTAACCGGTACGGTGCGGGTGAAGCTATTTAA
GGGGGCGGCAACGGTGGTGGGTCGCCAATCTCCTCATTCTCTCTATACGCCGGACCTGGCAACCTATGGCGCTGAAGATG
CCTTCGATCACAAAGCTGCTGAAGGATTTATCTACGTTTGGGGGCTACCAACTCGGGTCTGGTCGCAACAACAGCGATAA

Upstream 100 bases:

>100_bases
TACACTACGAGTTAGTGTCATCAATTACTGGTACTTGGTAGCAATCTGACCTTTAACCCTGCATAATAGGGAACGCAGTT
TTGCAGGGAAAAGAGCGATC

Downstream 100 bases:

>100_bases
GGACTGACCTGCTTAGCATCTCAGCAACCACTCCTGAAGTATGCATGGGGGTGAGAAGTGCGTTGCCACATTAACGCCGT
TAGCCAAAACAGAAAGAGTT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MGRAKKVVLAYSGGVDTSVCIPYLKHEWGVEEVITLAADLGQGDELEPIRKKALDAGAAQSLIADGTESFIADYAFPAIQ
ANALYENRYPLSTALARPLIAKLLVEAAEIHGADAVAHGCTGKGNDQVRFDVSITALNPDIKVLAPAREWGMSREETIAY
GEKYGVQSPVKKSSPYSIDRNLLGRSVEAGPLEDPWVEPLEEVYDLTKAIADTPDQPEYVDIDFAQGLPTQLNGQALSPV
ALVEQLNQIVGNHGVGRIDMVENRLVGIKSREIYEAPALLVLIQAHRELESLALTADVTHYKRGIEETYSQLVYNGLWFS
PLKDALDAFIQQTQKQVTGTVRVKLFKGAATVVGRQSPHSLYTPDLATYGAEDAFDHKAAEGFIYVWGLPTRVWSQQQR

Sequences:

>Translated_399_residues
MGRAKKVVLAYSGGVDTSVCIPYLKHEWGVEEVITLAADLGQGDELEPIRKKALDAGAAQSLIADGTESFIADYAFPAIQ
ANALYENRYPLSTALARPLIAKLLVEAAEIHGADAVAHGCTGKGNDQVRFDVSITALNPDIKVLAPAREWGMSREETIAY
GEKYGVQSPVKKSSPYSIDRNLLGRSVEAGPLEDPWVEPLEEVYDLTKAIADTPDQPEYVDIDFAQGLPTQLNGQALSPV
ALVEQLNQIVGNHGVGRIDMVENRLVGIKSREIYEAPALLVLIQAHRELESLALTADVTHYKRGIEETYSQLVYNGLWFS
PLKDALDAFIQQTQKQVTGTVRVKLFKGAATVVGRQSPHSLYTPDLATYGAEDAFDHKAAEGFIYVWGLPTRVWSQQQR
>Mature_398_residues
GRAKKVVLAYSGGVDTSVCIPYLKHEWGVEEVITLAADLGQGDELEPIRKKALDAGAAQSLIADGTESFIADYAFPAIQA
NALYENRYPLSTALARPLIAKLLVEAAEIHGADAVAHGCTGKGNDQVRFDVSITALNPDIKVLAPAREWGMSREETIAYG
EKYGVQSPVKKSSPYSIDRNLLGRSVEAGPLEDPWVEPLEEVYDLTKAIADTPDQPEYVDIDFAQGLPTQLNGQALSPVA
LVEQLNQIVGNHGVGRIDMVENRLVGIKSREIYEAPALLVLIQAHRELESLALTADVTHYKRGIEETYSQLVYNGLWFSP
LKDALDAFIQQTQKQVTGTVRVKLFKGAATVVGRQSPHSLYTPDLATYGAEDAFDHKAAEGFIYVWGLPTRVWSQQQR

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:
GO:0000166: Argininosuccinate synthase
GO:0004055: Argininosuccinate synthase
GO:0005524: Argininosuccinate synthase
GO:0005737: Argininosuccinate synthase
GO:0006526: Argininosuccinate synthase
GO:0008652: Argininosuccinate synthase
GO:0016874: Argininosuccinate synthase

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=411, Percent_Identity=39.9026763990268, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI16950633, Length=411, Percent_Identity=39.9026763990268, Blast_Score=287, Evalue=1e-77,
Organism=Escherichia coli, GI1789563, Length=391, Percent_Identity=28.3887468030691, Blast_Score=130, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324514, Length=410, Percent_Identity=37.5609756097561, Blast_Score=272, Evalue=5e-74,
Organism=Drosophila melanogaster, GI21358151, Length=400, Percent_Identity=38.25, Blast_Score=273, Evalue=1e-73,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_ACAM1 (B0CCQ2)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518531.1
- ProteinModelPortal:   B0CCQ2
- SMR:   B0CCQ2
- GeneID:   5683037
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_4234
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- BioCyc:   AMAR329726:AM1_4234-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 43645; Mature: 43514

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 38-38 BINDING 89-89 BINDING 119-119 BINDING 121-121 BINDING 125-125 BINDING 125-125 BINDING 126-126 BINDING 129-129 BINDING 177-177 BINDING 186-186 BINDING 262-262 BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRAKKVVLAYSGGVDTSVCIPYLKHEWGVEEVITLAADLGQGDELEPIRKKALDAGAAQ
CCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHH
SLIADGTESFIADYAFPAIQANALYENRYPLSTALARPLIAKLLVEAAEIHGADAVAHGC
HHHHCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
TGKGNDQVRFDVSITALNPDIKVLAPAREWGMSREETIAYGEKYGVQSPVKKSSPYSIDR
CCCCCCEEEEEEEEEEECCCCEEEECHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCH
NLLGRSVEAGPLEDPWVEPLEEVYDLTKAIADTPDQPEYVDIDFAQGLPTQLNGQALSPV
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHH
ALVEQLNQIVGNHGVGRIDMVENRLVGIKSREIYEAPALLVLIQAHRELESLALTADVTH
HHHHHHHHHHCCCCCCEEEHHHCHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
YKRGIEETYSQLVYNGLWFSPLKDALDAFIQQTQKQVTGTVRVKLFKGAATVVGRQSPHS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHCCCCCCC
LYTPDLATYGAEDAFDHKAAEGFIYVWGLPTRVWSQQQR
CCCCCHHHCCCCHHHHHHHCCCEEEEECCCHHHHCCCCC
>Mature Secondary Structure 
GRAKKVVLAYSGGVDTSVCIPYLKHEWGVEEVITLAADLGQGDELEPIRKKALDAGAAQ
CCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHH
SLIADGTESFIADYAFPAIQANALYENRYPLSTALARPLIAKLLVEAAEIHGADAVAHGC
HHHHCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
TGKGNDQVRFDVSITALNPDIKVLAPAREWGMSREETIAYGEKYGVQSPVKKSSPYSIDR
CCCCCCEEEEEEEEEEECCCCEEEECHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCH
NLLGRSVEAGPLEDPWVEPLEEVYDLTKAIADTPDQPEYVDIDFAQGLPTQLNGQALSPV
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHH
ALVEQLNQIVGNHGVGRIDMVENRLVGIKSREIYEAPALLVLIQAHRELESLALTADVTH
HHHHHHHHHHCCCCCCEEEHHHCHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
YKRGIEETYSQLVYNGLWFSPLKDALDAFIQQTQKQVTGTVRVKLFKGAATVVGRQSPHS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHCCCCCCC
LYTPDLATYGAEDAFDHKAAEGFIYVWGLPTRVWSQQQR
CCCCCHHHCCCCHHHHHHHCCCEEEEECCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA