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The map label for this gene is hisZ [H]

Identifier: 158337117

GI number: 158337117

Start: 4038724

End: 4039941

Strand: Direct

Name: hisZ [H]

Synonym: AM1_3991

Alternate gene names: 158337117

Gene position: 4038724-4039941 (Clockwise)

Preceding gene: 158337116

Following gene: 158337118

Centisome position: 62.1

GC content: 52.79

Gene sequence:

>1218_bases
TTGGTGACAATGTATCAACCTCCTGCTGGTGCCCGAGATTTACTGCCCTTAGATGTTGCCCAAAAATGCTGGATTGAGCA
GCGTCTGCTGCAGGTATTTCAGCGTTGGGGCTATCACCGCATTATTACCCCGACCTTAGAACGGTTGGATACTTTGTTAG
CGGGGGGAGCGGTAGACCCCCAAACCGTGATCCAAGTTTGGGATGCAGAAGAAGGAGTCTTAGGGTTGAGACCTGAGTTG
ACGGCCTCCATCGCTCGAGCTTCTGTGACTCGTATGGAAGGAGAGACTCACCCTCAGCGACTCTATTACAACGACAACAT
CTTTCGGCAACAGCCGGGTCATGCGAGCAGTCAGCATGAGTTTTACCAGGCGGGGGTAGAGTTGCTGGGGAGTAGCGGAT
TATTGGCAGATGCCGAGGTTCTGTTGCTCTTAGCGGATTGTCTCTCGGAACTAGGGGTTCAGAACTGGCATGTCCTTCTG
GGGGAAGCGGATTTAACCCGATCGCTGCTTCGCCCTTTTCCCGAAGCTGTGCGAGGATCTGTGCGCCGAGCCATTGCCCA
ACTGGATCGGGTGGGGCTAGCGGATTTGCCTTTGAGTCCAGAACTCAAGGAGCATGCTCTGTTTTTACTGGATTTGCGGG
GAGAACCGGCTCAGGTACTGCAAAAGGTATCACAGCTGCCCCTGGAACCACCCCAACATGAGGCGGTCACGAACCTCAAA
TCCTTGGTAGAAGTGTTGTCGAAGCAACCCTCACTTACCCTAGATCTGAGCTTGATTCAGACCTTTGACTATTACACAGG
TATCGTATTTGAGATTATCAGCGATGTAGAGTCGGGTCAGCGCTTACTAGGTCAGGGAGGGCGATACGATACGTTGCTAG
GCCTCTACCATCCCCAGAACGAAATGCTGCCTGGGATTGGTTTTTCCTTTAACGTGGAACATTTGCACCAGGTTCTCCTA
CCCCTTGGGATGTTGCCGAAACAGACGCCTGCCTCCCATTGGCTGGTGGTGGCGGAAACGGAATCTGCTATCGCAGCCGC
GTTTGACCATGCCCATCAGCTGCGTCAGCAAAATGCTCACCCTCCCCTCACTAGAGTTGAAGTGGAGCTATCCCAAAAGT
CACCCAATGACATTCGATCCACTGCCCGTCAACAGCGAATTGGGCAAATTGCTTGGGTCAATGCCCAAGGGTCTGTCACA
ATTGAATCGCTAGCGTAA

Upstream 100 bases:

>100_bases
ATTAGAAAAGAAAGCGCAACCCGCTGCAGCAAAACAAGCTCAAGCAAAGCAGCCGGAGCGTAAGAAGGGGCTGTTTGGTT
TGTTTGGTGGTAAGAAATAA

Downstream 100 bases:

>100_bases
TTCCCTTAGCGAACATTGCGATAGCCGTTATTCCATCGAGATTTATTTCATGCTCACGGTTGCCTTACCCAAAGGGGCGT
TACTGAAAGATAGTATTCGC

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MVTMYQPPAGARDLLPLDVAQKCWIEQRLLQVFQRWGYHRIITPTLERLDTLLAGGAVDPQTVIQVWDAEEGVLGLRPEL
TASIARASVTRMEGETHPQRLYYNDNIFRQQPGHASSQHEFYQAGVELLGSSGLLADAEVLLLLADCLSELGVQNWHVLL
GEADLTRSLLRPFPEAVRGSVRRAIAQLDRVGLADLPLSPELKEHALFLLDLRGEPAQVLQKVSQLPLEPPQHEAVTNLK
SLVEVLSKQPSLTLDLSLIQTFDYYTGIVFEIISDVESGQRLLGQGGRYDTLLGLYHPQNEMLPGIGFSFNVEHLHQVLL
PLGMLPKQTPASHWLVVAETESAIAAAFDHAHQLRQQNAHPPLTRVEVELSQKSPNDIRSTARQQRIGQIAWVNAQGSVT
IESLA

Sequences:

>Translated_405_residues
MVTMYQPPAGARDLLPLDVAQKCWIEQRLLQVFQRWGYHRIITPTLERLDTLLAGGAVDPQTVIQVWDAEEGVLGLRPEL
TASIARASVTRMEGETHPQRLYYNDNIFRQQPGHASSQHEFYQAGVELLGSSGLLADAEVLLLLADCLSELGVQNWHVLL
GEADLTRSLLRPFPEAVRGSVRRAIAQLDRVGLADLPLSPELKEHALFLLDLRGEPAQVLQKVSQLPLEPPQHEAVTNLK
SLVEVLSKQPSLTLDLSLIQTFDYYTGIVFEIISDVESGQRLLGQGGRYDTLLGLYHPQNEMLPGIGFSFNVEHLHQVLL
PLGMLPKQTPASHWLVVAETESAIAAAFDHAHQLRQQNAHPPLTRVEVELSQKSPNDIRSTARQQRIGQIAWVNAQGSVT
IESLA
>Mature_405_residues
MVTMYQPPAGARDLLPLDVAQKCWIEQRLLQVFQRWGYHRIITPTLERLDTLLAGGAVDPQTVIQVWDAEEGVLGLRPEL
TASIARASVTRMEGETHPQRLYYNDNIFRQQPGHASSQHEFYQAGVELLGSSGLLADAEVLLLLADCLSELGVQNWHVLL
GEADLTRSLLRPFPEAVRGSVRRAIAQLDRVGLADLPLSPELKEHALFLLDLRGEPAQVLQKVSQLPLEPPQHEAVTNLK
SLVEVLSKQPSLTLDLSLIQTFDYYTGIVFEIISDVESGQRLLGQGGRYDTLLGLYHPQNEMLPGIGFSFNVEHLHQVLL
PLGMLPKQTPASHWLVVAETESAIAAAFDHAHQLRQQNAHPPLTRVEVELSQKSPNDIRSTARQQRIGQIAWVNAQGSVT
IESLA

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=339, Percent_Identity=24.7787610619469, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI15029520, Length=341, Percent_Identity=24.3401759530792, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1788861, Length=370, Percent_Identity=27.8378378378378, Blast_Score=83, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24643061, Length=395, Percent_Identity=23.2911392405063, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24643059, Length=414, Percent_Identity=22.463768115942, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 44956; Mature: 44956

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTMYQPPAGARDLLPLDVAQKCWIEQRLLQVFQRWGYHRIITPTLERLDTLLAGGAVDP
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCCCH
QTVIQVWDAEEGVLGLRPELTASIARASVTRMEGETHPQRLYYNDNIFRQQPGHASSQHE
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCHHHH
FYQAGVELLGSSGLLADAEVLLLLADCLSELGVQNWHVLLGEADLTRSLLRPFPEAVRGS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCHHHHHHH
VRRAIAQLDRVGLADLPLSPELKEHALFLLDLRGEPAQVLQKVSQLPLEPPQHEAVTNLK
HHHHHHHHHHCCCCCCCCCCCHHHCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHH
SLVEVLSKQPSLTLDLSLIQTFDYYTGIVFEIISDVESGQRLLGQGGRYDTLLGLYHPQN
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHEEEEECCCC
EMLPGIGFSFNVEHLHQVLLPLGMLPKQTPASHWLVVAETESAIAAAFDHAHQLRQQNAH
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCC
PPLTRVEVELSQKSPNDIRSTARQQRIGQIAWVNAQGSVTIESLA
CCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEECC
>Mature Secondary Structure
MVTMYQPPAGARDLLPLDVAQKCWIEQRLLQVFQRWGYHRIITPTLERLDTLLAGGAVDP
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCCCH
QTVIQVWDAEEGVLGLRPELTASIARASVTRMEGETHPQRLYYNDNIFRQQPGHASSQHE
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCHHHH
FYQAGVELLGSSGLLADAEVLLLLADCLSELGVQNWHVLLGEADLTRSLLRPFPEAVRGS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCHHHHHHH
VRRAIAQLDRVGLADLPLSPELKEHALFLLDLRGEPAQVLQKVSQLPLEPPQHEAVTNLK
HHHHHHHHHHCCCCCCCCCCCHHHCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHH
SLVEVLSKQPSLTLDLSLIQTFDYYTGIVFEIISDVESGQRLLGQGGRYDTLLGLYHPQN
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHEEEEECCCC
EMLPGIGFSFNVEHLHQVLLPLGMLPKQTPASHWLVVAETESAIAAAFDHAHQLRQQNAH
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCC
PPLTRVEVELSQKSPNDIRSTARQQRIGQIAWVNAQGSVTIESLA
CCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA