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The map label for this gene is gloA [H]

Identifier: 158337106

GI number: 158337106

Start: 4029700

End: 4030125

Strand: Direct

Name: gloA [H]

Synonym: AM1_3979

Alternate gene names: 158337106

Gene position: 4029700-4030125 (Clockwise)

Preceding gene: 158337104

Following gene: 158337107

Centisome position: 61.96

GC content: 46.71

Gene sequence:

>426_bases
ATGAGAATTCTCCATACCATGCTCAGAGTGGCAGATCTTGATGCTTCTTTAGCATTCTACTGTGATGTCCTAGGAATGAA
GCTACTTCGTCGGAAAGATTACCCGAACGGAAAGTTTACGCTGGCCTTTGTGGGTTACGGAGATGAATCGGATAATACAG
TTCTAGAACTGACTTATAACTGGGGCGTCTCTGAATACGCTTTGGGAGACGCCTATGGCCATATTGCCATTGGCGTTGAC
GACATCTATAGTGCCTGCAACGACATTAAAACTCGTGGTGGAACAGTGACCCGAGAACCGGGTCCCATGAAACATGGCTC
AACGGTTATTGCCTTTGTAGAGGATCCCGATCACTATAAGGTAGAGTTGATTCAACTCAGCTCCAAAGCATCTGTGACTA
CGACAACCTCCACCTCTGTCAGTTGA

Upstream 100 bases:

>100_bases
ACGAGTATCTTCTTTAAGACATACTAGGTGCGATTGCGTCAGAATGTAACTAATGTGACGACCACCTCTTGACTTTATTA
ACTCTTAAACAAATAGTCTT

Downstream 100 bases:

>100_bases
GCAAGTCTTACCCTTGGTAATTGCTGGGTGAAAGCTTAGAGTCAAGCTTCCGCTCTTTTGAGTCGCAACAATGACAGCAA
ATTCAGCTCATGACCTTGAG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 141; Mature: 141

Protein sequence:

>141_residues
MRILHTMLRVADLDASLAFYCDVLGMKLLRRKDYPNGKFTLAFVGYGDESDNTVLELTYNWGVSEYALGDAYGHIAIGVD
DIYSACNDIKTRGGTVTREPGPMKHGSTVIAFVEDPDHYKVELIQLSSKASVTTTTSTSVS

Sequences:

>Translated_141_residues
MRILHTMLRVADLDASLAFYCDVLGMKLLRRKDYPNGKFTLAFVGYGDESDNTVLELTYNWGVSEYALGDAYGHIAIGVD
DIYSACNDIKTRGGTVTREPGPMKHGSTVIAFVEDPDHYKVELIQLSSKASVTTTTSTSVS
>Mature_141_residues
MRILHTMLRVADLDASLAFYCDVLGMKLLRRKDYPNGKFTLAFVGYGDESDNTVLELTYNWGVSEYALGDAYGHIAIGVD
DIYSACNDIKTRGGTVTREPGPMKHGSTVIAFVEDPDHYKVELIQLSSKASVTTTTSTSVS

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:
GO:0004462: Lactoylglutathione lyase
GO:0016829: Lactoylglutathione lyase
GO:0046872: Lactoylglutathione lyase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=156, Percent_Identity=35.8974358974359, Blast_Score=92, Evalue=1e-19,
Organism=Homo sapiens, GI217330598, Length=87, Percent_Identity=37.9310344827586, Blast_Score=63, Evalue=1e-10,
Organism=Escherichia coli, GI1787940, Length=125, Percent_Identity=64, Blast_Score=172, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=30.0751879699248, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=38.8489208633094, Blast_Score=92, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20129743, Length=150, Percent_Identity=35.3333333333333, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19920404, Length=141, Percent_Identity=32.6241134751773, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15462; Mature: 15462

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTMLRVADLDASLAFYCDVLGMKLLRRKDYPNGKFTLAFVGYGDESDNTVLELTYN
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEC
WGVSEYALGDAYGHIAIGVDDIYSACNDIKTRGGTVTREPGPMKHGSTVIAFVEDPDHYK
CCCCCEECCCCCCEEEECHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCEE
VELIQLSSKASVTTTTSTSVS
EEEEEECCCCEEEEECCCCCC
>Mature Secondary Structure
MRILHTMLRVADLDASLAFYCDVLGMKLLRRKDYPNGKFTLAFVGYGDESDNTVLELTYN
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEC
WGVSEYALGDAYGHIAIGVDDIYSACNDIKTRGGTVTREPGPMKHGSTVIAFVEDPDHYK
CCCCCEECCCCCCEEEECHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCEE
VELIQLSSKASVTTTTSTSVS
EEEEEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]