Click here to switch to the map view.

The map label for this gene is cysI [H]

Identifier: 158337007

GI number: 158337007

Start: 3932063

End: 3933997

Strand: Reverse

Name: cysI [H]

Synonym: AM1_3880

Alternate gene names: 158337007

Gene position: 3933997-3932063 (Counterclockwise)

Preceding gene: 158337008

Following gene: 158337006

Centisome position: 60.49

GC content: 51.06

Gene sequence:

>1935_bases
ATGGTCAATGCTCCCCCTAAACAACCGACTCCGCCTAAACTTTCCAAATTGGAAGGGATTAAAGAGCGCAGTCATCATCT
ACGCCATCCGTTAATTGCAGAACTCCAGACGGATGCGAGAAATTTGCCAGAAGAAGCCATTCAAATCCTCAAATTTCATG
GCTCCTATCAGCAAGATAATCGTGATAATCGAGCGAAAGGGCAGGAAAAAGACTATCAGTTTATGCTGCGGACTCGCAGT
CCCGGTGGATTTATCCCAGCACAGCTTTACGAAGCCCTGGATGAACTCAGCGAGACCTACGGCAACCACACCCTGCGAGC
CACCACCCGCCAAGGGTTCCAGATTCACGGAATTGTCAAGCAGAACCTAAAAACGGTCATGGCCGCCATCATTCACAGCA
TGGGGTCTACCCTAGGAGCCTGTGGAGATTTGAACCGCAATGTGATGGCCCCGCCAGCCCCCTATGTTAATCGTCCCGAC
TATGTCTATGCCCAAGAGTATGCCAATCGGATTGCCGATTTACTCACCCCCCAAACCGGCGCTTACTACGAGATTTGGTT
GGATGGGGAAAAATTCTTATCCGCAGAAGAGCATCCTGAGGTCAAAGCGGCTCGCCAGCGCAATACCAACGGCACTAACC
TAGACGATGACGAAGAGCCAATCTACGGGCAATATTACATGCCCCGGAAGTTCAAGATTGCCGTTACTGTCCCAGGCGAT
AACTCCATTGATCTCTACACCCAGGATGTGGGTCTAGTGGTTATCCTGGATGACCAGGACGAATTGCAAGGATTTAACGT
ATTTGTCGGGGGTGGCATGGGCCGCACCCACAAGAAAGAAGAGACTTTTGCTCGCATTGCCGATCCACTGGGCTTTGTGG
CCAAAGAGGATGTCTATGACCTAGTCAAAGCCATTGTGGCCACCCAACGAGATTATGGTGACCGTCAACAGCGTCGCCAT
GCCCGCATGAAATACCTGGTGAATGACTGGGGTATTGATAAGTTTCGCAGCGTTGTTGAGCAGTATTTTGGTAAGTCTGT
TGCGCCCAGTCGCCCCTTGCCTGAGTTTAAGTATGAAGATTACTTAGGCTGGCATGAACAGGGGGATGGTCAGCTCTTCT
TGGGCATTTCTATTGAGAATGGCCGGGTCTTTGACAATGGCAAGCTGCAGCTGAAGTCTGCCCTACGCCAAATTACGAAG
CAGTACCATCTGCCCATGCGGCTGACGGCTAATCACAATCTGATTATTTATAATATTCAGCCCAGTGACAAAGCGGCGAT
TCAAGGCATTTTGAACAATGCAGGGGTGCTAGCAGAGACGGAGATTGATCCGCTGGTGCGCTATGCAATGGCCTGTCCAG
CGTTGCCCACTTGCGGACTAGCCATTGCAGAATCAGAACGCGCCATTCCCGGCATTATTGATCGCATCCGCGCGCTGCTC
AATAAGCTGGATTTGAAAGAAGAGCATTTTGTCATCCGGATGACGGGCTGTCCAAATGGCTGTGCTCGTCCCTATATGGC
TGAGCTGGGATTTGTAGGGATAAAGCCAACGTCCTATCAGCTCTGGTTAGGGGGAAGCTCCAATCAGACTCGGCTCGCTA
GAACCTTTCTGGAAGTGATGCCCATTGACGAACTGGAAGCGACCCTAGAGCCGCTCTTGGTGTTCTTTAAGCAAAAGGGC
AAAACCGCTGAAAGCTTTGGTGATTTTTGCGATCGCATTGGCTTCGACGCCCTGCGGCAATATAGCGACACCTATGATCC
GGCGACCCAAACGGGCAAAATCGCCAGCGGTAAAGCGCGAGTCCGCCATCGGGTTAACCTGCGGGAAGATGTCTATAGCC
GTCTGAAAGAGACCGCCAGTGCCCAGGGCAAAACCCTGACTGAAGTCGCGTCTGATGCCATTGATACCTATTTAAAAAAT
CAATCTCAGACCTAA

Upstream 100 bases:

>100_bases
TGGAATTGCTCGGATATATTGACGTTAGCCTAGCCTTTTCTTAAGGGTAGAAATCGTTCGATTCGCTACCTAAACACTGT
CATCAACTGCCTATTTCCTC

Downstream 100 bases:

>100_bases
ATTGCACCTAATTTTAGGTGTAATAAAGCCATGGTGAAGATTGTCGTCATCGGCGGTGGGGCGGCTGGTTTCTGGGGGGC
GATTGCCTGTGCAGAAGCCT

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MVNAPPKQPTPPKLSKLEGIKERSHHLRHPLIAELQTDARNLPEEAIQILKFHGSYQQDNRDNRAKGQEKDYQFMLRTRS
PGGFIPAQLYEALDELSETYGNHTLRATTRQGFQIHGIVKQNLKTVMAAIIHSMGSTLGACGDLNRNVMAPPAPYVNRPD
YVYAQEYANRIADLLTPQTGAYYEIWLDGEKFLSAEEHPEVKAARQRNTNGTNLDDDEEPIYGQYYMPRKFKIAVTVPGD
NSIDLYTQDVGLVVILDDQDELQGFNVFVGGGMGRTHKKEETFARIADPLGFVAKEDVYDLVKAIVATQRDYGDRQQRRH
ARMKYLVNDWGIDKFRSVVEQYFGKSVAPSRPLPEFKYEDYLGWHEQGDGQLFLGISIENGRVFDNGKLQLKSALRQITK
QYHLPMRLTANHNLIIYNIQPSDKAAIQGILNNAGVLAETEIDPLVRYAMACPALPTCGLAIAESERAIPGIIDRIRALL
NKLDLKEEHFVIRMTGCPNGCARPYMAELGFVGIKPTSYQLWLGGSSNQTRLARTFLEVMPIDELEATLEPLLVFFKQKG
KTAESFGDFCDRIGFDALRQYSDTYDPATQTGKIASGKARVRHRVNLREDVYSRLKETASAQGKTLTEVASDAIDTYLKN
QSQT

Sequences:

>Translated_644_residues
MVNAPPKQPTPPKLSKLEGIKERSHHLRHPLIAELQTDARNLPEEAIQILKFHGSYQQDNRDNRAKGQEKDYQFMLRTRS
PGGFIPAQLYEALDELSETYGNHTLRATTRQGFQIHGIVKQNLKTVMAAIIHSMGSTLGACGDLNRNVMAPPAPYVNRPD
YVYAQEYANRIADLLTPQTGAYYEIWLDGEKFLSAEEHPEVKAARQRNTNGTNLDDDEEPIYGQYYMPRKFKIAVTVPGD
NSIDLYTQDVGLVVILDDQDELQGFNVFVGGGMGRTHKKEETFARIADPLGFVAKEDVYDLVKAIVATQRDYGDRQQRRH
ARMKYLVNDWGIDKFRSVVEQYFGKSVAPSRPLPEFKYEDYLGWHEQGDGQLFLGISIENGRVFDNGKLQLKSALRQITK
QYHLPMRLTANHNLIIYNIQPSDKAAIQGILNNAGVLAETEIDPLVRYAMACPALPTCGLAIAESERAIPGIIDRIRALL
NKLDLKEEHFVIRMTGCPNGCARPYMAELGFVGIKPTSYQLWLGGSSNQTRLARTFLEVMPIDELEATLEPLLVFFKQKG
KTAESFGDFCDRIGFDALRQYSDTYDPATQTGKIASGKARVRHRVNLREDVYSRLKETASAQGKTLTEVASDAIDTYLKN
QSQT
>Mature_644_residues
MVNAPPKQPTPPKLSKLEGIKERSHHLRHPLIAELQTDARNLPEEAIQILKFHGSYQQDNRDNRAKGQEKDYQFMLRTRS
PGGFIPAQLYEALDELSETYGNHTLRATTRQGFQIHGIVKQNLKTVMAAIIHSMGSTLGACGDLNRNVMAPPAPYVNRPD
YVYAQEYANRIADLLTPQTGAYYEIWLDGEKFLSAEEHPEVKAARQRNTNGTNLDDDEEPIYGQYYMPRKFKIAVTVPGD
NSIDLYTQDVGLVVILDDQDELQGFNVFVGGGMGRTHKKEETFARIADPLGFVAKEDVYDLVKAIVATQRDYGDRQQRRH
ARMKYLVNDWGIDKFRSVVEQYFGKSVAPSRPLPEFKYEDYLGWHEQGDGQLFLGISIENGRVFDNGKLQLKSALRQITK
QYHLPMRLTANHNLIIYNIQPSDKAAIQGILNNAGVLAETEIDPLVRYAMACPALPTCGLAIAESERAIPGIIDRIRALL
NKLDLKEEHFVIRMTGCPNGCARPYMAELGFVGIKPTSYQLWLGGSSNQTRLARTFLEVMPIDELEATLEPLLVFFKQKG
KTAESFGDFCDRIGFDALRQYSDTYDPATQTGKIASGKARVRHRVNLREDVYSRLKETASAQGKTLTEVASDAIDTYLKN
QSQT

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=582, Percent_Identity=37.4570446735395, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6322597, Length=583, Percent_Identity=40.1372212692967, Blast_Score=409, Evalue=1e-115,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR011787 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 72528; Mature: 72528

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNAPPKQPTPPKLSKLEGIKERSHHLRHPLIAELQTDARNLPEEAIQILKFHGSYQQDN
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
RDNRAKGQEKDYQFMLRTRSPGGFIPAQLYEALDELSETYGNHTLRATTRQGFQIHGIVK
CCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEHHHH
QNLKTVMAAIIHSMGSTLGACGDLNRNVMAPPAPYVNRPDYVYAQEYANRIADLLTPQTG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCC
AYYEIWLDGEKFLSAEEHPEVKAARQRNTNGTNLDDDEEPIYGQYYMPRKFKIAVTVPGD
CEEEEEECCHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEECCCEEEEEEEECCC
NSIDLYTQDVGLVVILDDQDELQGFNVFVGGGMGRTHKKEETFARIADPLGFVAKEDVYD
CCEEEEEECCCEEEEECCHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVKAIVATQRDYGDRQQRRHARMKYLVNDWGIDKFRSVVEQYFGKSVAPSRPLPEFKYED
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
YLGWHEQGDGQLFLGISIENGRVFDNGKLQLKSALRQITKQYHLPMRLTANHNLIIYNIQ
HCCCCCCCCCEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEC
PSDKAAIQGILNNAGVLAETEIDPLVRYAMACPALPTCGLAIAESERAIPGIIDRIRALL
CCHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHH
NKLDLKEEHFVIRMTGCPNGCARPYMAELGFVGIKPTSYQLWLGGSSNQTRLARTFLEVM
HHHCCCCCCEEEEECCCCCCCCCCHHHHCCCEECCCCCEEEEECCCCCHHHHHHHHHHHC
PIDELEATLEPLLVFFKQKGKTAESFGDFCDRIGFDALRQYSDTYDPATQTGKIASGKAR
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCHHH
VRHRVNLREDVYSRLKETASAQGKTLTEVASDAIDTYLKNQSQT
HHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVNAPPKQPTPPKLSKLEGIKERSHHLRHPLIAELQTDARNLPEEAIQILKFHGSYQQDN
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
RDNRAKGQEKDYQFMLRTRSPGGFIPAQLYEALDELSETYGNHTLRATTRQGFQIHGIVK
CCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEHHHH
QNLKTVMAAIIHSMGSTLGACGDLNRNVMAPPAPYVNRPDYVYAQEYANRIADLLTPQTG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCC
AYYEIWLDGEKFLSAEEHPEVKAARQRNTNGTNLDDDEEPIYGQYYMPRKFKIAVTVPGD
CEEEEEECCHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEECCCEEEEEEEECCC
NSIDLYTQDVGLVVILDDQDELQGFNVFVGGGMGRTHKKEETFARIADPLGFVAKEDVYD
CCEEEEEECCCEEEEECCHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVKAIVATQRDYGDRQQRRHARMKYLVNDWGIDKFRSVVEQYFGKSVAPSRPLPEFKYED
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
YLGWHEQGDGQLFLGISIENGRVFDNGKLQLKSALRQITKQYHLPMRLTANHNLIIYNIQ
HCCCCCCCCCEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEC
PSDKAAIQGILNNAGVLAETEIDPLVRYAMACPALPTCGLAIAESERAIPGIIDRIRALL
CCHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHH
NKLDLKEEHFVIRMTGCPNGCARPYMAELGFVGIKPTSYQLWLGGSSNQTRLARTFLEVM
HHHCCCCCCEEEEECCCCCCCCCCHHHHCCCEECCCCCEEEEECCCCCHHHHHHHHHHHC
PIDELEATLEPLLVFFKQKGKTAESFGDFCDRIGFDALRQYSDTYDPATQTGKIASGKAR
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCHHH
VRHRVNLREDVYSRLKETASAQGKTLTEVASDAIDTYLKNQSQT
HHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]