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The map label for this gene is prfC

Identifier: 158336972

GI number: 158336972

Start: 3897577

End: 3899202

Strand: Reverse

Name: prfC

Synonym: AM1_3845

Alternate gene names: 158336972

Gene position: 3899202-3897577 (Counterclockwise)

Preceding gene: 158336973

Following gene: 158336971

Centisome position: 59.95

GC content: 48.65

Gene sequence:

>1626_bases
ATGCCCACTGAACTGCAAACTGAGTTGGCTGAGGCGGTAGAAGCGCGCCGCAACTTTGCGATTATTTCTCACCCTGATGC
TGGTAAAACCACCTTGACGGAGAAACTACTGCTTTACGGAGGTGCCATCCATGAAGCAGGGGCAGTTAAGGCCAGACGGG
CCCAACGTCATGCGACGTCTGATTGGATGGAAATGGAGCAACAGCGGGGAATTTCGATTACCTCGACGGTGTTGCAGTTT
GCCTATCAGCACTGCCAAATTAATTTATTAGATACGCCGGGTCACCAAGATTTTAGTGAAGATACCTACCGTACCTTAGC
TGCTGCCGATAATGCAGTGATGCTGGTAGATGCAGCCAAAGGCTTAGAGCCGCAAACCCGAAAGTTGTTTGAGGTTTGTA
AGTTGCGGTCCCTGCCTATTTTTACCTTTATCAACAAGCTTGATCGACCTGGAAGAGAGCCATTAGAACTCCTGGATGAA
ATTGAGCAAGAGTTGGGGCTGCAGACCTATGCGGTCAATTGGCCCATTGGCATGGGCGATCGTTTTCAAGGGGTGTTTGA
TCGGCGCAGTCGTAAAATCCACCTCTTTCAGCGCAGCGATCATGGCAAACGAGAGGCCATCGACACCCAAATCGATTTAG
GCGATCCACAAATAGAGTCTTTGCTGGATCAGGAACTCTATTTTCAGCTTAAGGATGAGCTAGAGCTGATTGAGGAATTA
GGATCTCCCTTAGATCTGGAGCAAGTTCATAACGGCCAGATGACCCCCATCTTTTTCGGCAGTGCCATGACCAACTTCGG
GGTGGAGCTGTTTCTGGAAGCTTTTCTTGACTATGCCCTGAAACCTGGGGCCTATGAGAGTACCCAAGGCACCATTGAGC
CGACCTATGAGGATTTCTCAGGATTTGTGTTTAAGCTTCAGGCCAATATGGATCCGAAGCATCGCGATCGGGTCGCGTTT
ATCCGGGTGTGTTCGGGCAAATTCGAGAAAGATATGACCGTAAGCCATGCCCGTACCGGTAAAACAATCCGGCTTTCCCG
TCCCCAAAAGCTCTTTGCCCAGGGCCGTAATTCCCTAGAAGAAGCCTATCCCGGGGATATTATTGGTTTAAATAATCCGG
GAGTTTTTGCGATTGGCGACACCATTTTTAAGGGAAAGAAACTGGCCTATGAAGGGATTCCTTGCTTTTCACCCGAATTG
TTTGCCTACCTAAAAAATCCGAATCCCTCTAAATTCAAGCAATTTCAGAAAGGTGTTTCTGAGTTGCGGGAAGAAGGCGC
AGTACAAATTATGTACTCCGAAGATGAAATTAAGCGGGACCCGATCTTAGCGGCGGTGGGGCAACTGCAATTTGAAGTGG
TTCAATTCCGACTCCAAAATGAATATGGTGTTGAAACGCGTTTGGAATTATTGCCCTATTCTGTTGCCCGGTGGGTCAAA
GGAGGATGGTCGGCCTTAGAGTCGGTGGGGCGCATTTTTAATGCCATCACCGTCAAAGATAGCTGGGGGCGTCCAGTTTT
ACTGTTTAAGAATGAATGGAACTTGCACCAAGTGCACGGTGATCATCCTGATCTGGAACTGAGTGCGATCGCACCTTTAG
CCGTGGATCAAAACATGGTGAGCTGA

Upstream 100 bases:

>100_bases
CCGGGGTCGTCAATTCTGCTACTTTGACCGGATCGACAAGGGCTACCAGTCTCCTGACCTATAATAAAGTGTCTGAAAAC
TCCTCAATCTAGTCATCTGT

Downstream 100 bases:

>100_bases
GTAGTGCTGTTTCTATCAAAATCTAGTCAGCACTGGTTCTATTCCTCTAAAACTTTTAATAAAGTTGTTGTTAAATATGA
CAATCTGTTCCAAACCTGCA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MPTELQTELAEAVEARRNFAIISHPDAGKTTLTEKLLLYGGAIHEAGAVKARRAQRHATSDWMEMEQQRGISITSTVLQF
AYQHCQINLLDTPGHQDFSEDTYRTLAAADNAVMLVDAAKGLEPQTRKLFEVCKLRSLPIFTFINKLDRPGREPLELLDE
IEQELGLQTYAVNWPIGMGDRFQGVFDRRSRKIHLFQRSDHGKREAIDTQIDLGDPQIESLLDQELYFQLKDELELIEEL
GSPLDLEQVHNGQMTPIFFGSAMTNFGVELFLEAFLDYALKPGAYESTQGTIEPTYEDFSGFVFKLQANMDPKHRDRVAF
IRVCSGKFEKDMTVSHARTGKTIRLSRPQKLFAQGRNSLEEAYPGDIIGLNNPGVFAIGDTIFKGKKLAYEGIPCFSPEL
FAYLKNPNPSKFKQFQKGVSELREEGAVQIMYSEDEIKRDPILAAVGQLQFEVVQFRLQNEYGVETRLELLPYSVARWVK
GGWSALESVGRIFNAITVKDSWGRPVLLFKNEWNLHQVHGDHPDLELSAIAPLAVDQNMVS

Sequences:

>Translated_541_residues
MPTELQTELAEAVEARRNFAIISHPDAGKTTLTEKLLLYGGAIHEAGAVKARRAQRHATSDWMEMEQQRGISITSTVLQF
AYQHCQINLLDTPGHQDFSEDTYRTLAAADNAVMLVDAAKGLEPQTRKLFEVCKLRSLPIFTFINKLDRPGREPLELLDE
IEQELGLQTYAVNWPIGMGDRFQGVFDRRSRKIHLFQRSDHGKREAIDTQIDLGDPQIESLLDQELYFQLKDELELIEEL
GSPLDLEQVHNGQMTPIFFGSAMTNFGVELFLEAFLDYALKPGAYESTQGTIEPTYEDFSGFVFKLQANMDPKHRDRVAF
IRVCSGKFEKDMTVSHARTGKTIRLSRPQKLFAQGRNSLEEAYPGDIIGLNNPGVFAIGDTIFKGKKLAYEGIPCFSPEL
FAYLKNPNPSKFKQFQKGVSELREEGAVQIMYSEDEIKRDPILAAVGQLQFEVVQFRLQNEYGVETRLELLPYSVARWVK
GGWSALESVGRIFNAITVKDSWGRPVLLFKNEWNLHQVHGDHPDLELSAIAPLAVDQNMVS
>Mature_540_residues
PTELQTELAEAVEARRNFAIISHPDAGKTTLTEKLLLYGGAIHEAGAVKARRAQRHATSDWMEMEQQRGISITSTVLQFA
YQHCQINLLDTPGHQDFSEDTYRTLAAADNAVMLVDAAKGLEPQTRKLFEVCKLRSLPIFTFINKLDRPGREPLELLDEI
EQELGLQTYAVNWPIGMGDRFQGVFDRRSRKIHLFQRSDHGKREAIDTQIDLGDPQIESLLDQELYFQLKDELELIEELG
SPLDLEQVHNGQMTPIFFGSAMTNFGVELFLEAFLDYALKPGAYESTQGTIEPTYEDFSGFVFKLQANMDPKHRDRVAFI
RVCSGKFEKDMTVSHARTGKTIRLSRPQKLFAQGRNSLEEAYPGDIIGLNNPGVFAIGDTIFKGKKLAYEGIPCFSPELF
AYLKNPNPSKFKQFQKGVSELREEGAVQIMYSEDEIKRDPILAAVGQLQFEVVQFRLQNEYGVETRLELLPYSVARWVKG
GWSALESVGRIFNAITVKDSWGRPVLLFKNEWNLHQVHGDHPDLELSAIAPLAVDQNMVS

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:
GO:0000166: Peptide chain release factor 3
GO:0003924: Peptide chain release factor 3
GO:0005525: Peptide chain release factor 3
GO:0005737: Peptide chain release factor 3
GO:0006184: Peptide chain release factor 3
GO:0006412: Peptide chain release factor 3
GO:0006415: Peptide chain release factor 3
GO:0016149: Peptide chain release factor 3

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=487, Percent_Identity=30.1848049281314, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI19923640, Length=528, Percent_Identity=25.5681818181818, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI25306283, Length=391, Percent_Identity=28.1329923273657, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI25306287, Length=500, Percent_Identity=25.8, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI157426893, Length=156, Percent_Identity=32.6923076923077, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=34.3065693430657, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=30.8724832214765, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=37.0689655172414, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=37.0689655172414, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=37.0689655172414, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1790835, Length=470, Percent_Identity=50, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1789738, Length=495, Percent_Identity=28.4848484848485, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788922, Length=183, Percent_Identity=33.879781420765, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=35.5704697986577, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=501, Percent_Identity=26.1477045908184, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17556745, Length=480, Percent_Identity=24.7916666666667, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=34.8148148148148, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=150, Percent_Identity=30.6666666666667, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=150, Percent_Identity=30.6666666666667, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=490, Percent_Identity=26.3265306122449, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322359, Length=503, Percent_Identity=26.8389662027833, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6323320, Length=152, Percent_Identity=32.8947368421053, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324707, Length=179, Percent_Identity=32.4022346368715, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=179, Percent_Identity=32.4022346368715, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24582462, Length=488, Percent_Identity=30.9426229508197, Blast_Score=198, Evalue=9e-51,
Organism=Drosophila melanogaster, GI221458488, Length=514, Percent_Identity=23.9299610894942, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=38.3647798742138, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=30.0653594771242, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=30.0653594771242, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=30.0653594771242, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28574573, Length=142, Percent_Identity=34.5070422535211, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357743, Length=151, Percent_Identity=29.8013245033113, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_ACAM1 (B0C6Z1)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001518147.1
- ProteinModelPortal:   B0C6Z1
- SMR:   B0C6Z1
- GeneID:   5682649
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_3845
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- ProtClustDB:   PRK00741
- BioCyc:   AMAR329726:AM1_3845-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 61190; Mature: 61059

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTELQTELAEAVEARRNFAIISHPDAGKTTLTEKLLLYGGAIHEAGAVKARRAQRHATS
CCCHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHHCCH
DWMEMEQQRGISITSTVLQFAYQHCQINLLDTPGHQDFSEDTYRTLAAADNAVMLVDAAK
HHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCC
GLEPQTRKLFEVCKLRSLPIFTFINKLDRPGREPLELLDEIEQELGLQTYAVNWPIGMGD
CCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCC
RFQGVFDRRSRKIHLFQRSDHGKREAIDTQIDLGDPQIESLLDQELYFQLKDELELIEEL
HHHHHHHCCCCEEEEEECCCCCCHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GSPLDLEQVHNGQMTPIFFGSAMTNFGVELFLEAFLDYALKPGAYESTQGTIEPTYEDFS
CCCCCHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCC
GFVFKLQANMDPKHRDRVAFIRVCSGKFEKDMTVSHARTGKTIRLSRPQKLFAQGRNSLE
CEEEEEECCCCCCHHCCEEEEEECCCCCCCCCCHHHHCCCCEEEECCCHHHHHHCHHHHH
EAYPGDIIGLNNPGVFAIGDTIFKGKKLAYEGIPCFSPELFAYLKNPNPSKFKQFQKGVS
HHCCCCEEECCCCCEEEECCHHHCCCEEEECCCCCCCHHHHHEECCCCHHHHHHHHHHHH
ELREEGAVQIMYSEDEIKRDPILAAVGQLQFEVVQFRLQNEYGVETRLELLPYSVARWVK
HHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GGWSALESVGRIFNAITVKDSWGRPVLLFKNEWNLHQVHGDHPDLELSAIAPLAVDQNMV
HHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCEEEECCCCCCEEEEEECCHHCCCCCC
S
C
>Mature Secondary Structure 
PTELQTELAEAVEARRNFAIISHPDAGKTTLTEKLLLYGGAIHEAGAVKARRAQRHATS
CCHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHHCCH
DWMEMEQQRGISITSTVLQFAYQHCQINLLDTPGHQDFSEDTYRTLAAADNAVMLVDAAK
HHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCC
GLEPQTRKLFEVCKLRSLPIFTFINKLDRPGREPLELLDEIEQELGLQTYAVNWPIGMGD
CCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCC
RFQGVFDRRSRKIHLFQRSDHGKREAIDTQIDLGDPQIESLLDQELYFQLKDELELIEEL
HHHHHHHCCCCEEEEEECCCCCCHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GSPLDLEQVHNGQMTPIFFGSAMTNFGVELFLEAFLDYALKPGAYESTQGTIEPTYEDFS
CCCCCHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCC
GFVFKLQANMDPKHRDRVAFIRVCSGKFEKDMTVSHARTGKTIRLSRPQKLFAQGRNSLE
CEEEEEECCCCCCHHCCEEEEEECCCCCCCCCCHHHHCCCCEEEECCCHHHHHHCHHHHH
EAYPGDIIGLNNPGVFAIGDTIFKGKKLAYEGIPCFSPELFAYLKNPNPSKFKQFQKGVS
HHCCCCEEECCCCCEEEECCHHHCCCEEEECCCCCCCHHHHHEECCCCHHHHHHHHHHHH
ELREEGAVQIMYSEDEIKRDPILAAVGQLQFEVVQFRLQNEYGVETRLELLPYSVARWVK
HHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GGWSALESVGRIFNAITVKDSWGRPVLLFKNEWNLHQVHGDHPDLELSAIAPLAVDQNMV
HHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCEEEECCCCCCEEEEEECCHHCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA