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The map label for this gene is yieH [H]

Identifier: 158336968

GI number: 158336968

Start: 3894900

End: 3895565

Strand: Reverse

Name: yieH [H]

Synonym: AM1_3841

Alternate gene names: 158336968

Gene position: 3895565-3894900 (Counterclockwise)

Preceding gene: 158336969

Following gene: 158336967

Centisome position: 59.9

GC content: 46.1

Gene sequence:

>666_bases
ATGAAGTTTGAGCTGGCTATTTTTGACTGTGATGGGGTGCTGATTGATAGTGAACTGCTGGCTAATCGTATAGATGTAGA
GTGCTTACAGGCGTTAGGGATTGAAATTGACCTTGATGACTATATAGCTGAGTATGTGGGCAAAAGTGCTGTAGCTGTCT
CCCACAGTATTGAAGAGCGATATACGGTTCAGTTGCCTGGAGATTTTTGGTCACAAGTAGAACAGAAGACGCTAAAACAA
TTTCAAGCTGAACTACAGCCAATTCCCCATGTCGTTGAGCTACTGACTGTCCTAAACAAAAAGTGTGTAGCGTCGAGTAG
TTCTGCTGATCGACTTAATCGAACCCTAGGGATGACAGGATTGTATAAGTACTTCAGCCCCCATATTTTTAGTGCTGAGC
AAGTGAGGCATGGCAAGCCTGCTCCTGACTTGTTTCTGTTTGCTGCTCAACAGATGCAGGTGAACCCACAAGATTGTGTG
GTGATAGAAGATAGTTGTCATGGCGTCCAGGCGGGGATAGATGCGGGCATGACAGTTGTCGGCTTTTCGGGTGGGAGTCA
TATCAGATCAGGGCATCTAGACAAGTTAATGGATGCAGGGGCCGCAATGGTGTTTGCAGATATGGGATCACTTTTCGCTG
GATTAAGTCAGCTGGTACCCCAATGA

Upstream 100 bases:

>100_bases
TACAGCTTAAAGGCGGCAAGTGGAAGGGCTGCTTCCATGTACCTAGAGCTTTGTTTCTCTGCTGGCAACAGTTTGAAAAG
CTGAGTGCCAAAAACTTGGC

Downstream 100 bases:

>100_bases
GATGGGGGATGATTGTGTCGAGACATTCAGCAAAGCAAGCGGTTGTCTGGTTCCCTAGTCAGCAACAAATATAAGACGGG
GAATAGGAGAGCTATGGGTT

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKFELAIFDCDGVLIDSELLANRIDVECLQALGIEIDLDDYIAEYVGKSAVAVSHSIEERYTVQLPGDFWSQVEQKTLKQ
FQAELQPIPHVVELLTVLNKKCVASSSSADRLNRTLGMTGLYKYFSPHIFSAEQVRHGKPAPDLFLFAAQQMQVNPQDCV
VIEDSCHGVQAGIDAGMTVVGFSGGSHIRSGHLDKLMDAGAAMVFADMGSLFAGLSQLVPQ

Sequences:

>Translated_221_residues
MKFELAIFDCDGVLIDSELLANRIDVECLQALGIEIDLDDYIAEYVGKSAVAVSHSIEERYTVQLPGDFWSQVEQKTLKQ
FQAELQPIPHVVELLTVLNKKCVASSSSADRLNRTLGMTGLYKYFSPHIFSAEQVRHGKPAPDLFLFAAQQMQVNPQDCV
VIEDSCHGVQAGIDAGMTVVGFSGGSHIRSGHLDKLMDAGAAMVFADMGSLFAGLSQLVPQ
>Mature_221_residues
MKFELAIFDCDGVLIDSELLANRIDVECLQALGIEIDLDDYIAEYVGKSAVAVSHSIEERYTVQLPGDFWSQVEQKTLKQ
FQAELQPIPHVVELLTVLNKKCVASSSSADRLNRTLGMTGLYKYFSPHIFSAEQVRHGKPAPDLFLFAAQQMQVNPQDCV
VIEDSCHGVQAGIDAGMTVVGFSGGSHIRSGHLDKLMDAGAAMVFADMGSLFAGLSQLVPQ

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=188, Percent_Identity=30.3191489361702, Blast_Score=103, Evalue=6e-24,
Organism=Escherichia coli, GI1789046, Length=177, Percent_Identity=31.638418079096, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI87082080, Length=190, Percent_Identity=27.8947368421053, Blast_Score=60, Evalue=9e-11,
Organism=Drosophila melanogaster, GI17137324, Length=184, Percent_Identity=31.5217391304348, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24092; Mature: 24092

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFELAIFDCDGVLIDSELLANRIDVECLQALGIEIDLDDYIAEYVGKSAVAVSHSIEER
CCEEEEEEECCCEEECHHHHHHHHHHHHHHHCCCEECHHHHHHHHHCCHHHHHHHHHCCC
YTVQLPGDFWSQVEQKTLKQFQAELQPIPHVVELLTVLNKKCVASSSSADRLNRTLGMTG
EEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHH
LYKYFSPHIFSAEQVRHGKPAPDLFLFAAQQMQVNPQDCVVIEDSCHGVQAGIDAGMTVV
HHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHCCCCEEE
GFSGGSHIRSGHLDKLMDAGAAMVFADMGSLFAGLSQLVPQ
EECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFELAIFDCDGVLIDSELLANRIDVECLQALGIEIDLDDYIAEYVGKSAVAVSHSIEER
CCEEEEEEECCCEEECHHHHHHHHHHHHHHHCCCEECHHHHHHHHHCCHHHHHHHHHCCC
YTVQLPGDFWSQVEQKTLKQFQAELQPIPHVVELLTVLNKKCVASSSSADRLNRTLGMTG
EEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHH
LYKYFSPHIFSAEQVRHGKPAPDLFLFAAQQMQVNPQDCVVIEDSCHGVQAGIDAGMTVV
HHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHCCCCEEE
GFSGGSHIRSGHLDKLMDAGAAMVFADMGSLFAGLSQLVPQ
EECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]