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The map label for this gene is menE [H]

Identifier: 158336886

GI number: 158336886

Start: 3813491

End: 3814897

Strand: Direct

Name: menE [H]

Synonym: AM1_3754

Alternate gene names: 158336886

Gene position: 3813491-3814897 (Clockwise)

Preceding gene: 158336885

Following gene: 158336890

Centisome position: 58.64

GC content: 48.47

Gene sequence:

>1407_bases
TTGGGTTCTCCCCAAGCAATTTGGCACAGGGAAGACTGGATTCTGGGCCTTAATCATCAGCGTTGGTTAGAGCGAATTCG
ACAACGACAACGAGAGCTACAAGCGGTGATGGAGCGAGAGGGCTATCCCAGAGTCCTGTTAGTTCAGGCTGATCCACTAG
AGTTTTTAGCTGGATTGATGGCGGCGTGTTTATGTGATTGTCCTGTATTTCTCGGGAATCCCCATTGGCAACGTTCTGAA
TGGCAACAGGTGTTGCTGCTAGTTCAACCCCATGTTATTTGGGATGCCTCTCAGTTGCCTCCGGTAGATATCCCAAAACC
AGCCCCAGCTAAGCAAGAAGAGAAGGGATGGATTTTAATCTCTACCGGGGGCTCATCGGGACAGGTTCGATTTGCGATTC
ATACTTGGGAGACTCTAACTGCCTCGGTTGAAGGCTGCCAAAATCATTTTTTCGGTGGTGAATTGGATGCCATCAATTCC
TGCTGCTGGTTACCCCTGTATCACGTCAGTGGGTTGATGCAGTTTATGCGATCGCTCCTTACATACGGTAAACTCCTCCT
CCTCCCACGCCACTGTTTTTCTCCTGAAATCTCCTTGATGCCAGACATAGCGGCTTTTCTAGATCCGCAATCAGAGCAAC
CTTATTCCTTTTTTCTCTCCCTCGTTCCTACCCAACTCCAGCGATTCTTGCAACAGGGCGGCCCTCGTTTACAATGGCTA
GCCCGATTTCACACTATTTTGGTGGGGGGCGGTCCTGCATGGCCCGCTTTACTCCAGGGGGCTCGCCAGCACCAGTTGCG
GTTGGCTTTAACCTATGGCATGACGGAAACTGCTGCTCAAGTGGCGACATTAGATCCCGATCAATTCCTGCTCGGAGCAG
CTCACAGTGGCCATGTTTTGCCCCATGCTCAAATTGCCATTGAGCATCAGCAACACCAGGGCATTGTGGCTCCTCAAACA
GGACAGGTGGTGATTGAATGTACATCTCTTTGTAAAGGATATTACCCGCAATTATTAGCCTCACCTCGTAAATTTTTTAC
GGATGATTTAGGGTATTTCAATAACCAAGGAGCATTGCATATTGTCGGTCGTTTAAGTCGCAAGATTATCTCTGGGGGGG
AGAATGTTTTTCCCGAAGAAATAGAAAAATTAATTTTAAGTACAGGCCTCGTAAAAGATGTGACGGTGTTGGGCCAACCT
CATCCCGATTGGGGCCAAATCATTACCGCCATTGTGGTTCCTGCAGCAGACTCCTTTTCCCTAATCGCATTGCAGACTTG
TCTGAAGAATCAACTCAGCCCGTTTAAACAACCAAAGCAATGGTATGTCTTGCCTCAGCTGCCCCGTAACCCTCAAGGTA
AAATCGACCAATCACAGATGAGGAAACTACTTCAGGTTGATCAGTAG

Upstream 100 bases:

>100_bases
CCCCAACGAGCCATTGGGTTTGGTGTTTGCCATTGGTTGTCCCCTGATGAATAACGCTTCGTTCTCGGTTGATCCGGTTA
AGCCGTCCAGTGCTCACTTA

Downstream 100 bases:

>100_bases
TGACTACAGGGACGGAAATTCTGACATTGACCCATCTAATTTTATTGATATATTTCACTCTGTTAACCCCCGCGAATAAG
AGTGAACTTTAGTAAAATCC

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MGSPQAIWHREDWILGLNHQRWLERIRQRQRELQAVMEREGYPRVLLVQADPLEFLAGLMAACLCDCPVFLGNPHWQRSE
WQQVLLLVQPHVIWDASQLPPVDIPKPAPAKQEEKGWILISTGGSSGQVRFAIHTWETLTASVEGCQNHFFGGELDAINS
CCWLPLYHVSGLMQFMRSLLTYGKLLLLPRHCFSPEISLMPDIAAFLDPQSEQPYSFFLSLVPTQLQRFLQQGGPRLQWL
ARFHTILVGGGPAWPALLQGARQHQLRLALTYGMTETAAQVATLDPDQFLLGAAHSGHVLPHAQIAIEHQQHQGIVAPQT
GQVVIECTSLCKGYYPQLLASPRKFFTDDLGYFNNQGALHIVGRLSRKIISGGENVFPEEIEKLILSTGLVKDVTVLGQP
HPDWGQIITAIVVPAADSFSLIALQTCLKNQLSPFKQPKQWYVLPQLPRNPQGKIDQSQMRKLLQVDQ

Sequences:

>Translated_468_residues
MGSPQAIWHREDWILGLNHQRWLERIRQRQRELQAVMEREGYPRVLLVQADPLEFLAGLMAACLCDCPVFLGNPHWQRSE
WQQVLLLVQPHVIWDASQLPPVDIPKPAPAKQEEKGWILISTGGSSGQVRFAIHTWETLTASVEGCQNHFFGGELDAINS
CCWLPLYHVSGLMQFMRSLLTYGKLLLLPRHCFSPEISLMPDIAAFLDPQSEQPYSFFLSLVPTQLQRFLQQGGPRLQWL
ARFHTILVGGGPAWPALLQGARQHQLRLALTYGMTETAAQVATLDPDQFLLGAAHSGHVLPHAQIAIEHQQHQGIVAPQT
GQVVIECTSLCKGYYPQLLASPRKFFTDDLGYFNNQGALHIVGRLSRKIISGGENVFPEEIEKLILSTGLVKDVTVLGQP
HPDWGQIITAIVVPAADSFSLIALQTCLKNQLSPFKQPKQWYVLPQLPRNPQGKIDQSQMRKLLQVDQ
>Mature_467_residues
GSPQAIWHREDWILGLNHQRWLERIRQRQRELQAVMEREGYPRVLLVQADPLEFLAGLMAACLCDCPVFLGNPHWQRSEW
QQVLLLVQPHVIWDASQLPPVDIPKPAPAKQEEKGWILISTGGSSGQVRFAIHTWETLTASVEGCQNHFFGGELDAINSC
CWLPLYHVSGLMQFMRSLLTYGKLLLLPRHCFSPEISLMPDIAAFLDPQSEQPYSFFLSLVPTQLQRFLQQGGPRLQWLA
RFHTILVGGGPAWPALLQGARQHQLRLALTYGMTETAAQVATLDPDQFLLGAAHSGHVLPHAQIAIEHQQHQGIVAPQTG
QVVIECTSLCKGYYPQLLASPRKFFTDDLGYFNNQGALHIVGRLSRKIISGGENVFPEEIEKLILSTGLVKDVTVLGQPH
PDWGQIITAIVVPAADSFSLIALQTCLKNQLSPFKQPKQWYVLPQLPRNPQGKIDQSQMRKLLQVDQ

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=312, Percent_Identity=24.3589743589744, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI187761345, Length=410, Percent_Identity=25.609756097561, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI187761343, Length=410, Percent_Identity=25.609756097561, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI122937307, Length=260, Percent_Identity=26.1538461538462, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788595, Length=359, Percent_Identity=27.5766016713092, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788107, Length=351, Percent_Identity=23.3618233618234, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693145, Length=234, Percent_Identity=24.3589743589744, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI32563687, Length=310, Percent_Identity=26.7741935483871, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=353, Percent_Identity=26.9121813031161, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71994703, Length=370, Percent_Identity=22.1621621621622, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71994694, Length=370, Percent_Identity=22.1621621621622, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71994690, Length=370, Percent_Identity=22.1621621621622, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17560308, Length=367, Percent_Identity=24.5231607629428, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=323, Percent_Identity=26.0061919504644, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17538037, Length=205, Percent_Identity=26.3414634146341, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=307, Percent_Identity=25.7328990228013, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24581924, Length=365, Percent_Identity=26.027397260274, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648260, Length=319, Percent_Identity=24.7648902821317, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355181, Length=376, Percent_Identity=22.0744680851064, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 52550; Mature: 52419

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSPQAIWHREDWILGLNHQRWLERIRQRQRELQAVMEREGYPRVLLVQADPLEFLAGLM
CCCCCCEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHH
AACLCDCPVFLGNPHWQRSEWQQVLLLVQPHVIWDASQLPPVDIPKPAPAKQEEKGWILI
HHHHHCCCHHCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEE
STGGSSGQVRFAIHTWETLTASVEGCQNHFFGGELDAINSCCWLPLYHVSGLMQFMRSLL
EECCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
TYGKLLLLPRHCFSPEISLMPDIAAFLDPQSEQPYSFFLSLVPTQLQRFLQQGGPRLQWL
HHHHHHCCCHHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
ARFHTILVGGGPAWPALLQGARQHQLRLALTYGMTETAAQVATLDPDQFLLGAAHSGHVL
HHHHHHEECCCCCCHHHHCCHHHHEEEEEEEECCHHHHHHHHCCCCCHHEEECCCCCCCC
PHAQIAIEHQQHQGIVAPQTGQVVIECTSLCKGYYPQLLASPRKFFTDDLGYFNNQGALH
CCHHEEEEHHHCCCEECCCCCCEEEEHHHHHCCCCHHHHCCCHHHHHHHHCCCCCCCCEE
IVGRLSRKIISGGENVFPEEIEKLILSTGLVKDVTVLGQPHPDWGQIITAIVVPAADSFS
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCHH
LIALQTCLKNQLSPFKQPKQWYVLPQLPRNPQGKIDQSQMRKLLQVDQ
HHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
GSPQAIWHREDWILGLNHQRWLERIRQRQRELQAVMEREGYPRVLLVQADPLEFLAGLM
CCCCCEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHH
AACLCDCPVFLGNPHWQRSEWQQVLLLVQPHVIWDASQLPPVDIPKPAPAKQEEKGWILI
HHHHHCCCHHCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEE
STGGSSGQVRFAIHTWETLTASVEGCQNHFFGGELDAINSCCWLPLYHVSGLMQFMRSLL
EECCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
TYGKLLLLPRHCFSPEISLMPDIAAFLDPQSEQPYSFFLSLVPTQLQRFLQQGGPRLQWL
HHHHHHCCCHHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
ARFHTILVGGGPAWPALLQGARQHQLRLALTYGMTETAAQVATLDPDQFLLGAAHSGHVL
HHHHHHEECCCCCCHHHHCCHHHHEEEEEEEECCHHHHHHHHCCCCCHHEEECCCCCCCC
PHAQIAIEHQQHQGIVAPQTGQVVIECTSLCKGYYPQLLASPRKFFTDDLGYFNNQGALH
CCHHEEEEHHHCCCEECCCCCCEEEEHHHHHCCCCHHHHCCCHHHHHHHHCCCCCCCCEE
IVGRLSRKIISGGENVFPEEIEKLILSTGLVKDVTVLGQPHPDWGQIITAIVVPAADSFS
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCHH
LIALQTCLKNQLSPFKQPKQWYVLPQLPRNPQGKIDQSQMRKLLQVDQ
HHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA