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The map label for this gene is rpoE [H]

Identifier: 158336732

GI number: 158336732

Start: 3655783

End: 3656445

Strand: Direct

Name: rpoE [H]

Synonym: AM1_3600

Alternate gene names: 158336732

Gene position: 3655783-3656445 (Clockwise)

Preceding gene: 158336727

Following gene: 158336733

Centisome position: 56.21

GC content: 50.38

Gene sequence:

>663_bases
ATGACAGCCAGTCTTCAGGTATCTTGGTCAAGGGTTAGGGTTGAGGATTCAGAACCTCACGTGCAAATACCAGCAGCGAA
ACTTGGCAACTCTGAGCTGGTGCAACTTTGTCAAGCTGGGTTGCGCCCCGATCGTGTTGCTTTTACAGAACTCCTCAGAC
GCTACCAGTCTCATGTCGATCGGGTGCTGTATCATCTCGCTCCCGATTGGGATGATCGCGCCGATTTAGCCCAAGAAGTG
TGGATCCGAGTCTATCGAAATATCAGCCGTCTCAAGGAACCAGCGAAGTTCCGAGGGTGGTTGAGTCGGATTACCACCAA
TTTGTTCTACGACGAACTTCGCAAGCGTAAGCGCCACCGCAGCCCCCTGTCTCTGGACGCACGGCTGACCATGGAAGATG
GTGATATTGCTTGGGAAATTGCGTCAGGAGAGCCGGGACCGGATGAAAGCTTGGCCACCCATGAGTTTTACGGTCAACTT
AATGATGCGATCTCAGAACTCCCAGAAGCGTTTCGTACCACGATTGTTTTGCGAGAAATTGAAGGCTTAGCCTACGAAGA
AATTGCTGAAATTACGGGTGTTTCCTTGGGAACGGTTAAATCTCGGATTGCTCGAGCTCGTTTGCGGCTACAAGGGCAAT
TGCAAAATTATCTTAAAGGTTGA

Upstream 100 bases:

>100_bases
GAAGTATCTCACATTATAAGAAACCAACTTGCGGTCATGGGAACCCCCTATTTATCCCCTGCGTCAGAAAGGGCAGGTGT
AATTATGGTCAATTCCATTC

Downstream 100 bases:

>100_bases
CGTTAAATTCTTCATCAAAGTTGATCATTCTAAGGATATAGAAGTAAAACCTTTAACACTTCTTTGTCGGTCTCCTAAAC
ATACGTTAGATTTAAACTAG

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MTASLQVSWSRVRVEDSEPHVQIPAAKLGNSELVQLCQAGLRPDRVAFTELLRRYQSHVDRVLYHLAPDWDDRADLAQEV
WIRVYRNISRLKEPAKFRGWLSRITTNLFYDELRKRKRHRSPLSLDARLTMEDGDIAWEIASGEPGPDESLATHEFYGQL
NDAISELPEAFRTTIVLREIEGLAYEEIAEITGVSLGTVKSRIARARLRLQGQLQNYLKG

Sequences:

>Translated_220_residues
MTASLQVSWSRVRVEDSEPHVQIPAAKLGNSELVQLCQAGLRPDRVAFTELLRRYQSHVDRVLYHLAPDWDDRADLAQEV
WIRVYRNISRLKEPAKFRGWLSRITTNLFYDELRKRKRHRSPLSLDARLTMEDGDIAWEIASGEPGPDESLATHEFYGQL
NDAISELPEAFRTTIVLREIEGLAYEEIAEITGVSLGTVKSRIARARLRLQGQLQNYLKG
>Mature_219_residues
TASLQVSWSRVRVEDSEPHVQIPAAKLGNSELVQLCQAGLRPDRVAFTELLRRYQSHVDRVLYHLAPDWDDRADLAQEVW
IRVYRNISRLKEPAKFRGWLSRITTNLFYDELRKRKRHRSPLSLDARLTMEDGDIAWEIASGEPGPDESLATHEFYGQLN
DAISELPEAFRTTIVLREIEGLAYEEIAEITGVSLGTVKSRIARARLRLQGQLQNYLKG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=195, Percent_Identity=32.8205128205128, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 25254; Mature: 25123

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00622 HTH_LUXR_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASLQVSWSRVRVEDSEPHVQIPAAKLGNSELVQLCQAGLRPDRVAFTELLRRYQSHVD
CCCCEEEEHHEEEEECCCCCEECCHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RVLYHLAPDWDDRADLAQEVWIRVYRNISRLKEPAKFRGWLSRITTNLFYDELRKRKRHR
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPLSLDARLTMEDGDIAWEIASGEPGPDESLATHEFYGQLNDAISELPEAFRTTIVLREI
CCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGLAYEEIAEITGVSLGTVKSRIARARLRLQGQLQNYLKG
HCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TASLQVSWSRVRVEDSEPHVQIPAAKLGNSELVQLCQAGLRPDRVAFTELLRRYQSHVD
CCCEEEEHHEEEEECCCCCEECCHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RVLYHLAPDWDDRADLAQEVWIRVYRNISRLKEPAKFRGWLSRITTNLFYDELRKRKRHR
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPLSLDARLTMEDGDIAWEIASGEPGPDESLATHEFYGQLNDAISELPEAFRTTIVLREI
CCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGLAYEEIAEITGVSLGTVKSRIARARLRLQGQLQNYLKG
HCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]