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The map label for this gene is folE [H]

Identifier: 158336194

GI number: 158336194

Start: 3078790

End: 3079425

Strand: Direct

Name: folE [H]

Synonym: AM1_3056

Alternate gene names: 158336194

Gene position: 3078790-3079425 (Clockwise)

Preceding gene: 158336184

Following gene: 158336195

Centisome position: 47.34

GC content: 53.62

Gene sequence:

>636_bases
ATGACCGTGCCTTCCATCCCTTCATTTGTCCCTGAGACGCTTACCACTGAAGCTCAGCCCATCGTCTCGGAGGCCGAAAT
GGAGGCCGCCGTCCGCACGTTGTTGCTGGGTATGGGTGAAGATCCCGATCGCGAAGGCCTGCGAGATACCCCTAAGCGAG
TGGTTAAATCTTTAAAATTTCTGTCTTCGGGTTATCAGCAATCCCTGGATGAATTGCTAAATGGGGCCGTCTTCTCCGAA
GATCTAGATGAAATGGTACTGGTGCGCGATATTGACCTCTTTAGTTCCTGCGAACATCACATCTTGCCCATCATCGGCAA
AGCCCATGTGGCCTATATCCCCAACGGCAAAGTGATTGGCCTGTCGAAAATCGCCCGCATCTGTGAAATGTATGCTCGGC
GACTTCAGGTCCAAGAACGGTTAACCGCCCAAATTGCTGATGCCTTACAGGGTCTCCTGAAGCCCCAAGGGGTAGCTGTC
GTGGTAGAAGCAAGCCATATGTGTATGGTGATGCGTGGGGTACAAAAACCCGGATCGTGGACCTCCACCAGCGCGATGCG
AGGCGTCTTTTCCAGTGATGCCCGCACCCGCCAAGAGTTCTTTAACTTGGTTGCCCACGCACCCGCCATTCGCTAG

Upstream 100 bases:

>100_bases
TAATGATTATCATTATATCTGTATAATGTCAGTTTGCTTTTGGATTAAAGCCGACTGGATCTCGAAGTTCCTCTTATCCC
TTTCCGCATTAGGAGTTTGT

Downstream 100 bases:

>100_bases
TTCCGGCTTCAGCCGATCAGCACTATGCTTCCCGTCCCTGGACTCTTTGAATACCGTCTGCCCGTCACCCTGATCACGGG
CTTTTTGGGGAGTGGTAAAA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I 2; GTP-CH-I 2 [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTVPSIPSFVPETLTTEAQPIVSEAEMEAAVRTLLLGMGEDPDREGLRDTPKRVVKSLKFLSSGYQQSLDELLNGAVFSE
DLDEMVLVRDIDLFSSCEHHILPIIGKAHVAYIPNGKVIGLSKIARICEMYARRLQVQERLTAQIADALQGLLKPQGVAV
VVEASHMCMVMRGVQKPGSWTSTSAMRGVFSSDARTRQEFFNLVAHAPAIR

Sequences:

>Translated_211_residues
MTVPSIPSFVPETLTTEAQPIVSEAEMEAAVRTLLLGMGEDPDREGLRDTPKRVVKSLKFLSSGYQQSLDELLNGAVFSE
DLDEMVLVRDIDLFSSCEHHILPIIGKAHVAYIPNGKVIGLSKIARICEMYARRLQVQERLTAQIADALQGLLKPQGVAV
VVEASHMCMVMRGVQKPGSWTSTSAMRGVFSSDARTRQEFFNLVAHAPAIR
>Mature_210_residues
TVPSIPSFVPETLTTEAQPIVSEAEMEAAVRTLLLGMGEDPDREGLRDTPKRVVKSLKFLSSGYQQSLDELLNGAVFSED
LDEMVLVRDIDLFSSCEHHILPIIGKAHVAYIPNGKVIGLSKIARICEMYARRLQVQERLTAQIADALQGLLKPQGVAVV
VEASHMCMVMRGVQKPGSWTSTSAMRGVFSSDARTRQEFFNLVAHAPAIR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:
GO:0000166: GTP cyclohydrolase 1 1
GO:0003934: GTP cyclohydrolase 1 1
GO:0005525: GTP cyclohydrolase 1 1
GO:0005737: GTP cyclohydrolase 1 1
GO:0006730: GTP cyclohydrolase 1 1
GO:0016787: GTP cyclohydrolase 1 1
GO:0046654: GTP cyclohydrolase 1 1
GO:0046872: GTP cyclohydrolase 1 1

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=178, Percent_Identity=56.7415730337079, Blast_Score=225, Evalue=2e-59,
Organism=Homo sapiens, GI4503949, Length=178, Percent_Identity=56.7415730337079, Blast_Score=225, Evalue=2e-59,
Organism=Homo sapiens, GI66932970, Length=148, Percent_Identity=52.027027027027, Blast_Score=175, Evalue=2e-44,
Organism=Homo sapiens, GI66932972, Length=139, Percent_Identity=54.6762589928058, Blast_Score=174, Evalue=5e-44,
Organism=Escherichia coli, GI1788476, Length=218, Percent_Identity=39.4495412844037, Blast_Score=129, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17560486, Length=178, Percent_Identity=58.9887640449438, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321706, Length=180, Percent_Identity=58.8888888888889, Blast_Score=223, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24656782, Length=194, Percent_Identity=56.1855670103093, Blast_Score=214, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24656791, Length=194, Percent_Identity=56.1855670103093, Blast_Score=213, Evalue=6e-56,
Organism=Drosophila melanogaster, GI24656796, Length=194, Percent_Identity=56.1855670103093, Blast_Score=213, Evalue=6e-56,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 23192; Mature: 23061

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPSIPSFVPETLTTEAQPIVSEAEMEAAVRTLLLGMGEDPDREGLRDTPKRVVKSLKF
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHH
LSSGYQQSLDELLNGAVFSEDLDEMVLVRDIDLFSSCEHHILPIIGKAHVAYIPNGKVIG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCEEC
LSKIARICEMYARRLQVQERLTAQIADALQGLLKPQGVAVVVEASHMCMVMRGVQKPGSW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCCC
TSTSAMRGVFSSDARTRQEFFNLVAHAPAIR
CHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TVPSIPSFVPETLTTEAQPIVSEAEMEAAVRTLLLGMGEDPDREGLRDTPKRVVKSLKF
CCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHH
LSSGYQQSLDELLNGAVFSEDLDEMVLVRDIDLFSSCEHHILPIIGKAHVAYIPNGKVIG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCEEC
LSKIARICEMYARRLQVQERLTAQIADALQGLLKPQGVAVVVEASHMCMVMRGVQKPGSW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCCC
TSTSAMRGVFSSDARTRQEFFNLVAHAPAIR
CHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]