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The map label for this gene is ycdT [H]

Identifier: 158336078

GI number: 158336078

Start: 2972007

End: 2972387

Strand: Direct

Name: ycdT [H]

Synonym: AM1_2939

Alternate gene names: 158336078

Gene position: 2972007-2972387 (Clockwise)

Preceding gene: 158336077

Following gene: 158336079

Centisome position: 45.7

GC content: 49.34

Gene sequence:

>381_bases
ATGACGCTTGCTTCCCTAGATATCGACCATTTTAAGGCCATTAATGATGCCTTTGGCCATGCTACTGGCGACCAAGTGCT
GCGGGATATCAGCGGCTTGCTCAAAGAACGATTGCGCCGTGTCGATCAGGTCTTTAGAATTGGAGGCGAAGAATTTCTGT
TGCTCTTTTATGGGTCAGATACCGAGAGTGGATATCTGGTCGCGGAAGAACTACGCCATTTAATTGGAAGCCAACCATTT
CTCCCGGATCATTCCGTAACGGTCAGCATTGGTGTAGCCACCCTACATCCTAAAGAAGACTGGACGACCTGGATGCAGCG
AAGCGATGATTATCTCTATCGGGCCAAATCGGCAGGGCGCAATCAAGTGATGTGCAGGTAA

Upstream 100 bases:

>100_bases
TCAAGCCATCACAGATCCACTGACTGGGTTACTGAACCGAACGTCCCTCCATGAGACCCTTGAGCAAGCTATCGCCCAAA
GTAAGCGGATGGGCATTTCT

Downstream 100 bases:

>100_bases
TCTCTTTTTCCAGGTATAACCAAAATATGAAACCGCTACGCCATCATCTAACCCGTATCTGTCTTGTTCTGGGACTGATT
GTTTCCCTAGTTATGGCTGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MTLASLDIDHFKAINDAFGHATGDQVLRDISGLLKERLRRVDQVFRIGGEEFLLLFYGSDTESGYLVAEELRHLIGSQPF
LPDHSVTVSIGVATLHPKEDWTTWMQRSDDYLYRAKSAGRNQVMCR

Sequences:

>Translated_126_residues
MTLASLDIDHFKAINDAFGHATGDQVLRDISGLLKERLRRVDQVFRIGGEEFLLLFYGSDTESGYLVAEELRHLIGSQPF
LPDHSVTVSIGVATLHPKEDWTTWMQRSDDYLYRAKSAGRNQVMCR
>Mature_125_residues
TLASLDIDHFKAINDAFGHATGDQVLRDISGLLKERLRRVDQVFRIGGEEFLLLFYGSDTESGYLVAEELRHLIGSQPFL
PDHSVTVSIGVATLHPKEDWTTWMQRSDDYLYRAKSAGRNQVMCR

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=131, Percent_Identity=38.1679389312977, Blast_Score=89, Evalue=1e-19,
Organism=Escherichia coli, GI87081881, Length=124, Percent_Identity=40.3225806451613, Blast_Score=85, Evalue=1e-18,
Organism=Escherichia coli, GI1786584, Length=126, Percent_Identity=38.8888888888889, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI87082007, Length=130, Percent_Identity=38.4615384615385, Blast_Score=83, Evalue=6e-18,
Organism=Escherichia coli, GI145693134, Length=127, Percent_Identity=33.8582677165354, Blast_Score=82, Evalue=1e-17,
Organism=Escherichia coli, GI1787802, Length=130, Percent_Identity=36.9230769230769, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI1787816, Length=127, Percent_Identity=32.2834645669291, Blast_Score=77, Evalue=5e-16,
Organism=Escherichia coli, GI1788381, Length=126, Percent_Identity=32.5396825396825, Blast_Score=69, Evalue=8e-14,
Organism=Escherichia coli, GI1787056, Length=126, Percent_Identity=36.5079365079365, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1787541, Length=123, Percent_Identity=39.0243902439024, Blast_Score=66, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 14313; Mature: 14182

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLASLDIDHFKAINDAFGHATGDQVLRDISGLLKERLRRVDQVFRIGGEEFLLLFYGSD
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
TESGYLVAEELRHLIGSQPFLPDHSVTVSIGVATLHPKEDWTTWMQRSDDYLYRAKSAGR
CCCCEEHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHCCCCHHHHHCCCCC
NQVMCR
CCCCCC
>Mature Secondary Structure 
TLASLDIDHFKAINDAFGHATGDQVLRDISGLLKERLRRVDQVFRIGGEEFLLLFYGSD
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
TESGYLVAEELRHLIGSQPFLPDHSVTVSIGVATLHPKEDWTTWMQRSDDYLYRAKSAGR
CCCCEEHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHCCCCHHHHHCCCCC
NQVMCR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]