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The map label for this gene is lacZ [H]

Identifier: 158335956

GI number: 158335956

Start: 2852816

End: 2855101

Strand: Direct

Name: lacZ [H]

Synonym: AM1_2814

Alternate gene names: 158335956

Gene position: 2852816-2855101 (Clockwise)

Preceding gene: 158335953

Following gene: 158335957

Centisome position: 43.86

GC content: 51.71

Gene sequence:

>2286_bases
ATGTTCCTAGGATGCTTAACTGCCACGCATCCTAGACTGATCGCCAATCCTCACTTATTCCATGCCCAAGCTTACTGGAC
TCCGACGGTTACCCAGACGGTCAATCAAACGTCCTTGGCGGGAACCTGGGAGTTTCAAGCCGATCCGCAGCCCCCGGCAG
ACAAGCTAGTGCTACAGCCAAGTGGCCCAGATCTCCCACCCCTACCGCAAGGACAATGGCAAAAGATTACCGTGCCCTCC
AACTGGTATCTACAGGGGCAAGATGCATCGGGGGTGGTTTGGTATCGCCATCAATTTCGCGCTAACGATCGATGGCGTCG
AAAGATTACCCGCCTCGTTTTTTCGGGGGTGGACTACACCGCCGATGTTTGGCTAAACGGACAATATTTAGGGTTTCACG
AGGGCTATTTTCAGCCCTTTTCCTTTGATGTGTCTAAGGCCCTAATGTTTACAGGTCAGAATGAATTATTAGTCCGGGTC
AATAGCCCCTTGGAGCCTGAAGGCCAGGATTGGTCCCTGCGTAAACGGTTGATTAAAGGCATTTTTAGCCACCACGATAC
CCGTCCGGGTGGGGCATGGACCGATCGCGGCCAGGAGAAAAACACCGGGGGTATTTGGGCACCCGTATTTTTGCAAGTCT
CGGATCAAGTCGCCATTGAAACACTTCAAATCACCCCGGAGTTAGATACCGACCATCAACAGGCAACGGCCAAGGTGGAT
CTGGTGGTCAACTCTCCAGGGCTGGGCAAGACCACTCTAGATGCCCATATGAAGTTAGAGCCAGAGAACTTTGAAGGGGT
ACCGATTGGCCCCATTCAAGTGCAGCGAACCCTGCATCAAGGGGCCAATCACCTAAAGCTAGAGGTTCCCGTAGATGACC
CGAAACTCTGGTGGTCTTGGGAGCATGGAGACCCCAATTTATATCGCCTCACGGTACAGTTAGCCGATGGCAATCGGCTC
CTGGATAAGGCTTCTGATGTGTTTGGATTTCGCACCATTCAGTTTGACCCGACAGAAAAAATCTGGCGACTGAATGGTCG
CCGCCTATTTTTGCGAGGCACCAATTACATTGCCACCCAATGGCTGAGTGAAATGACGCCGGACAAATACCAGACGGATA
TTGACCTGATGAAAGCGGTCAATATCAATACGGTGCGGGTTCATGCCCATGTGATCGGCAAGGAGTTTTATGAACTGTGC
GATCGCAACGGCGTATTTATCTGGCAAGACTTCCCCTTGCAGTGGGGCTACGAAGATACGCCCCAATTTCTCAAAGAAGC
CACCCGCCAAGCCAAGGACATGATTGGTTACCTTTACAACCATCCGTCGATTCTGGCCTGGAGTCTGCATAATGAGCCAC
CCTGGGATGCGGACTGGATGAAGTATAAGTACGAGTCCTATAATCCAGAGCAAAATCGCGAGCTAGATGCCAAACTGTTT
GCCAATCTCCAAGGAGAAGACCCGACCCGTCTGCTCCATCCCGTATCTACCGTCGGGGAGCATCCCTGGTGGGGCTGGTA
TTCCCACACGATGTATAAATATGCAGAACCAACTACAGAAGCGATTATTTCCGAATTTGGCGCTCAAGCTTTACCCCATA
TAGATTCCCTGCGGCGCATCTTTACGGAGGACGAGCTATGGCCGGATACAGAAGCGGAATGGGATAAGTGGCTCTATCAC
AACTTTCAACGCCATGAAACCTTCAACATTGCCAAGGTGCCCATGGGCAACACCACCCAGGAGTTTATCGACAACACCCA
GCAGTACCAGTCCCAACTGATTCAGTTTGCCGCCGAAGCCTATCGTCGCCAGCGCTATCAGCCCGTTTCATCCATTTTTC
AATTTATGTTTGTGGAGGATTGGCCGTCCATGAACTGGGGCATTGTCGATTACTGGCGACAGCCTAAGCCCGGCCATGAA
GCCCTGAAATGGGCCTATCAGCCGGTCTTACCCAGTATTGCGTGGCCGCAACTCACCTGGACCTTGGGAGAAACGGTAGA
GCTGGAACTCTGGGCCATCAACGATTTGTGGAAAGACTATCCAGAGGCCATCTTTACCTATGCCCTGCGCCAGAATCAAC
AACGCCTTAAATCCGGTCAAAGTCGGATTAATTTGGCTGCCGATAGCAGTCAGCCTGTAGATAAGATCTTTTTCCAGCCC
CAGAACTTGGGTAGCTACACCGTCACCGCCACCCTCAAGGATCGGCTAGGAACGGTCTTGGGAGAAAACCAGTTTGTGTT
TGATGTGATTGATCGGGGGGATCAAACCAATGAGTCGACGTCGTAA

Upstream 100 bases:

>100_bases
ACGGTAGGGGAACTCTAGGGCCATAAATTGACTTGTCTCTGGTAGCAAGATGTCGAAAAGGCGACGATACCGACCGTTTT
TAATTCTTTTTGCGATAGGA

Downstream 100 bases:

>100_bases
GTTACGGACGCTATTGATCTGTTTTATCGCCTCAGTCGCCTTAACGATTGGGGTGGCTAAGTGGGTCTGTAGCCAGCAGC
TCGCCGAGGACTTTGCACCT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 761; Mature: 761

Protein sequence:

>761_residues
MFLGCLTATHPRLIANPHLFHAQAYWTPTVTQTVNQTSLAGTWEFQADPQPPADKLVLQPSGPDLPPLPQGQWQKITVPS
NWYLQGQDASGVVWYRHQFRANDRWRRKITRLVFSGVDYTADVWLNGQYLGFHEGYFQPFSFDVSKALMFTGQNELLVRV
NSPLEPEGQDWSLRKRLIKGIFSHHDTRPGGAWTDRGQEKNTGGIWAPVFLQVSDQVAIETLQITPELDTDHQQATAKVD
LVVNSPGLGKTTLDAHMKLEPENFEGVPIGPIQVQRTLHQGANHLKLEVPVDDPKLWWSWEHGDPNLYRLTVQLADGNRL
LDKASDVFGFRTIQFDPTEKIWRLNGRRLFLRGTNYIATQWLSEMTPDKYQTDIDLMKAVNINTVRVHAHVIGKEFYELC
DRNGVFIWQDFPLQWGYEDTPQFLKEATRQAKDMIGYLYNHPSILAWSLHNEPPWDADWMKYKYESYNPEQNRELDAKLF
ANLQGEDPTRLLHPVSTVGEHPWWGWYSHTMYKYAEPTTEAIISEFGAQALPHIDSLRRIFTEDELWPDTEAEWDKWLYH
NFQRHETFNIAKVPMGNTTQEFIDNTQQYQSQLIQFAAEAYRRQRYQPVSSIFQFMFVEDWPSMNWGIVDYWRQPKPGHE
ALKWAYQPVLPSIAWPQLTWTLGETVELELWAINDLWKDYPEAIFTYALRQNQQRLKSGQSRINLAADSSQPVDKIFFQP
QNLGSYTVTATLKDRLGTVLGENQFVFDVIDRGDQTNESTS

Sequences:

>Translated_761_residues
MFLGCLTATHPRLIANPHLFHAQAYWTPTVTQTVNQTSLAGTWEFQADPQPPADKLVLQPSGPDLPPLPQGQWQKITVPS
NWYLQGQDASGVVWYRHQFRANDRWRRKITRLVFSGVDYTADVWLNGQYLGFHEGYFQPFSFDVSKALMFTGQNELLVRV
NSPLEPEGQDWSLRKRLIKGIFSHHDTRPGGAWTDRGQEKNTGGIWAPVFLQVSDQVAIETLQITPELDTDHQQATAKVD
LVVNSPGLGKTTLDAHMKLEPENFEGVPIGPIQVQRTLHQGANHLKLEVPVDDPKLWWSWEHGDPNLYRLTVQLADGNRL
LDKASDVFGFRTIQFDPTEKIWRLNGRRLFLRGTNYIATQWLSEMTPDKYQTDIDLMKAVNINTVRVHAHVIGKEFYELC
DRNGVFIWQDFPLQWGYEDTPQFLKEATRQAKDMIGYLYNHPSILAWSLHNEPPWDADWMKYKYESYNPEQNRELDAKLF
ANLQGEDPTRLLHPVSTVGEHPWWGWYSHTMYKYAEPTTEAIISEFGAQALPHIDSLRRIFTEDELWPDTEAEWDKWLYH
NFQRHETFNIAKVPMGNTTQEFIDNTQQYQSQLIQFAAEAYRRQRYQPVSSIFQFMFVEDWPSMNWGIVDYWRQPKPGHE
ALKWAYQPVLPSIAWPQLTWTLGETVELELWAINDLWKDYPEAIFTYALRQNQQRLKSGQSRINLAADSSQPVDKIFFQP
QNLGSYTVTATLKDRLGTVLGENQFVFDVIDRGDQTNESTS
>Mature_761_residues
MFLGCLTATHPRLIANPHLFHAQAYWTPTVTQTVNQTSLAGTWEFQADPQPPADKLVLQPSGPDLPPLPQGQWQKITVPS
NWYLQGQDASGVVWYRHQFRANDRWRRKITRLVFSGVDYTADVWLNGQYLGFHEGYFQPFSFDVSKALMFTGQNELLVRV
NSPLEPEGQDWSLRKRLIKGIFSHHDTRPGGAWTDRGQEKNTGGIWAPVFLQVSDQVAIETLQITPELDTDHQQATAKVD
LVVNSPGLGKTTLDAHMKLEPENFEGVPIGPIQVQRTLHQGANHLKLEVPVDDPKLWWSWEHGDPNLYRLTVQLADGNRL
LDKASDVFGFRTIQFDPTEKIWRLNGRRLFLRGTNYIATQWLSEMTPDKYQTDIDLMKAVNINTVRVHAHVIGKEFYELC
DRNGVFIWQDFPLQWGYEDTPQFLKEATRQAKDMIGYLYNHPSILAWSLHNEPPWDADWMKYKYESYNPEQNRELDAKLF
ANLQGEDPTRLLHPVSTVGEHPWWGWYSHTMYKYAEPTTEAIISEFGAQALPHIDSLRRIFTEDELWPDTEAEWDKWLYH
NFQRHETFNIAKVPMGNTTQEFIDNTQQYQSQLIQFAAEAYRRQRYQPVSSIFQFMFVEDWPSMNWGIVDYWRQPKPGHE
ALKWAYQPVLPSIAWPQLTWTLGETVELELWAINDLWKDYPEAIFTYALRQNQQRLKSGQSRINLAADSSQPVDKIFFQP
QNLGSYTVTATLKDRLGTVLGENQFVFDVIDRGDQTNESTS

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI84798622, Length=628, Percent_Identity=23.4076433121019, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI48994920, Length=435, Percent_Identity=25.7471264367816, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1786539, Length=450, Percent_Identity=26.4444444444444, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 88291; Mature: 88291

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLGCLTATHPRLIANPHLFHAQAYWTPTVTQTVNQTSLAGTWEFQADPQPPADKLVLQP
CEEEEEECCCCCEECCCCEEEEEEEECCHHHHHCCCCCCCEEEEECCCCCCCCCCEEECC
SGPDLPPLPQGQWQKITVPSNWYLQGQDASGVVWYRHQFRANDRWRRKITRLVFSGVDYT
CCCCCCCCCCCCCEEEECCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEE
ADVWLNGQYLGFHEGYFQPFSFDVSKALMFTGQNELLVRVNSPLEPEGQDWSLRKRLIKG
EEEEECCEEEEECCCCCCCCCCCHHHEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHH
IFSHHDTRPGGAWTDRGQEKNTGGIWAPVFLQVSDQVAIETLQITPELDTDHQQATAKVD
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEEECCCCCCCCCCCEEEEE
LVVNSPGLGKTTLDAHMKLEPENFEGVPIGPIQVQRTLHQGANHLKLEVPVDDPKLWWSW
EEEECCCCCCEEECEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEEE
EHGDPNLYRLTVQLADGNRLLDKASDVFGFRTIQFDPTEKIWRLNGRRLFLRGTNYIATQ
ECCCCCEEEEEEEECCCCHHHHHHHHHHEEEEEEECCCHHHHCCCCCEEEEECCCHHHHH
WLSEMTPDKYQTDIDLMKAVNINTVRVHAHVIGKEFYELCDRNGVFIWQDFPLQWGYEDT
HHHHCCCCHHHCCHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
PQFLKEATRQAKDMIGYLYNHPSILAWSLHNEPPWDADWMKYKYESYNPEQNRELDAKLF
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHEEEECCCCCCCCCCCCHHHH
ANLQGEDPTRLLHPVSTVGEHPWWGWYSHTMYKYAEPTTEAIISEFGAQALPHIDSLRRI
CCCCCCCHHHHHHHHHHHCCCCCCCHHHCCCEECCCCHHHHHHHHHCHHHCCCHHHHHHH
FTEDELWPDTEAEWDKWLYHNFQRHETFNIAKVPMGNTTQEFIDNTQQYQSQLIQFAAEA
HCCCCCCCCCCHHHHHHHHHCHHHCCCEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHH
YRRQRYQPVSSIFQFMFVEDWPSMNWGIVDYWRQPKPGHEALKWAYQPVLPSIAWPQLTW
HHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEE
TLGETVELELWAINDLWKDYPEAIFTYALRQNQQRLKSGQSRINLAADSSQPVDKIFFQP
ECCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHEECC
QNLGSYTVTATLKDRLGTVLGENQFVFDVIDRGDQTNESTS
CCCCCEEEEEEEHHHHHHHHCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MFLGCLTATHPRLIANPHLFHAQAYWTPTVTQTVNQTSLAGTWEFQADPQPPADKLVLQP
CEEEEEECCCCCEECCCCEEEEEEEECCHHHHHCCCCCCCEEEEECCCCCCCCCCEEECC
SGPDLPPLPQGQWQKITVPSNWYLQGQDASGVVWYRHQFRANDRWRRKITRLVFSGVDYT
CCCCCCCCCCCCCEEEECCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEE
ADVWLNGQYLGFHEGYFQPFSFDVSKALMFTGQNELLVRVNSPLEPEGQDWSLRKRLIKG
EEEEECCEEEEECCCCCCCCCCCHHHEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHH
IFSHHDTRPGGAWTDRGQEKNTGGIWAPVFLQVSDQVAIETLQITPELDTDHQQATAKVD
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEEECCCCCCCCCCCEEEEE
LVVNSPGLGKTTLDAHMKLEPENFEGVPIGPIQVQRTLHQGANHLKLEVPVDDPKLWWSW
EEEECCCCCCEEECEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEEE
EHGDPNLYRLTVQLADGNRLLDKASDVFGFRTIQFDPTEKIWRLNGRRLFLRGTNYIATQ
ECCCCCEEEEEEEECCCCHHHHHHHHHHEEEEEEECCCHHHHCCCCCEEEEECCCHHHHH
WLSEMTPDKYQTDIDLMKAVNINTVRVHAHVIGKEFYELCDRNGVFIWQDFPLQWGYEDT
HHHHCCCCHHHCCHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
PQFLKEATRQAKDMIGYLYNHPSILAWSLHNEPPWDADWMKYKYESYNPEQNRELDAKLF
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHEEEECCCCCCCCCCCCHHHH
ANLQGEDPTRLLHPVSTVGEHPWWGWYSHTMYKYAEPTTEAIISEFGAQALPHIDSLRRI
CCCCCCCHHHHHHHHHHHCCCCCCCHHHCCCEECCCCHHHHHHHHHCHHHCCCHHHHHHH
FTEDELWPDTEAEWDKWLYHNFQRHETFNIAKVPMGNTTQEFIDNTQQYQSQLIQFAAEA
HCCCCCCCCCCHHHHHHHHHCHHHCCCEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHH
YRRQRYQPVSSIFQFMFVEDWPSMNWGIVDYWRQPKPGHEALKWAYQPVLPSIAWPQLTW
HHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEE
TLGETVELELWAINDLWKDYPEAIFTYALRQNQQRLKSGQSRINLAADSSQPVDKIFFQP
ECCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHEECC
QNLGSYTVTATLKDRLGTVLGENQFVFDVIDRGDQTNESTS
CCCCCEEEEEEEHHHHHHHHCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA