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The map label for this gene is proS

Identifier: 158335473

GI number: 158335473

Start: 2321283

End: 2323085

Strand: Direct

Name: proS

Synonym: AM1_2321

Alternate gene names: 158335473

Gene position: 2321283-2323085 (Clockwise)

Preceding gene: 158335471

Following gene: 158335474

Centisome position: 35.69

GC content: 49.47

Gene sequence:

>1803_bases
ATGCGGCTATCTCAAATGCTGTTTGTGACGCTGCGAGAAGACCCAGCGGAAGCAGAAATCCCCAGCCATAAATTACTCTT
GCGGGCCGGGTATATTCGCCGTGTAGGCAGTGGGATTTATGCCTATTTACCGTTGATGTGGCGTGTGTTGCAAAAGGTGT
CTCAGATTGTCCGGGAAGAAATGGATGCAGCTGGGGCTCAGGAATGTTTGCTGCCCCAAATTCAGCCCGCTGAGTTATGG
CAAGAATCAGGTCGATGGGACACCTATACGCAAGCAGAAGGGATTATGTTTGCCCTCCAGGATCGCCAGGACCGGGAGTT
GGGATTAGGGCCGACCCACGAAGAGGTGATTACTGCGGTCGCCAGGGATATGATTCGCTCCTACCGTCAGCTTCCTTTGA
ACCTTTATCAAATTCAGACCAAATTCCGTGATGAGATTCGCCCTCGGTTTGGCTTGATGCGAGGACGGGAATTCATTATG
AAGGACGCCTATTCTTTTTCCACGGACGAAGCCGATCTCAAAATTATTTACAGTAAGATGCATGATGCTTACTGCAATAT
TCTCCAACGATCTGGCTTAGCGTATCGCGCAGTTGATGCTGATTCTGGAGCCATTGGCGGCTCTGGGTCCCAGGAGTTTA
TGGTATTGGCCGAAGCTGGGGAAGATGAGGTTCTCTACACAGACGATGGTAAATATGCGGCCAATGTAGAAAAAGCAGTG
TCCCGACCTGCCGATGCTCAGCCTTCTTCCTTCAAGTCCGTAGAAAAGAAAGAAACGCCCAAGACAGATACCATTGCCAA
ACTCTGTGATTGCTTGCAATGCTCGCCGACTCAGGTTGTCAAAACGGTGCTCTACGAAGCGGTTTATGACAATGGTCAAA
CCCTTCTGGCTTTAGTCAGTTTGCGGGGCGATCAATCCGTCAATGAGGTGAAACTACAGAATGAGTTGGTCCAGTTAGGC
GACGTGGTGAAAGGCAAAGCTCTCATTGCTCTCACTGTTGCGGAAGGAGGAACCAAATGGGCGAGCCAGCCTCTACCCCT
GGGGTATATTGCCCCTGATTTAGCAGATAGCTATATTCAAAAGTCCAAACAGGTGCATGGCAAGTTTGTTCGCTTAGTCG
ATCAAACAGCAGTCGATCTCAAGAATTTTGTGACGGGGGCTAACGAGGTTGGCATCCACCAAGTCGGGGTCAACTGGAAA
ACAGATATTCCTTTACCCAAACATGTGGTGGATATTCGCACAGCTCAGGTGGGAGATCGAGCGGTACACGATCCCAAGCA
AACCCTACAAACTGCTCGAGGCATCGAAATTGGTCATATCTTTCAATTAGGTACGAAGTACTCCCAGGCGTTAGGGGCGA
CCTATACGAATGAAGCGGGTAAAGAACAGCCATTAGTGATGGGTTGCTATGGCGTTGGGGTCTCTCGTCTGGCCCAAGCT
GCTGTGGAACAATCCTATGACAAAGATGGGATTGTCTGGCCAGTTGCGATCGCACCGTATCACGCCATCATTATTATTCC
CAATGGGAAAGATAGCGACCAGGTAGAGGCCGCTGAAAAACTCTACAGCGAGCTCAATGCTGCAGGCATAGAAACCTTGC
TAGACGATCGGACAGAACGAGCGGGGGTGAAATTCAAAGACGCTGACTTAATTGGAATTCCCTATCGAATTGTGACGGGA
CGATCCTTGAAGGAAGGCAACGTTGAGATGGTAGAACGAGCCACCCACGACGCGCATCAAATTGCGTTAGATCAGGTTTT
ACCCACCCTTAAAGAATATTTAGCGAAAGCCATTTCTACTTAG

Upstream 100 bases:

>100_bases
CTCTGATTTTGGCAAAACTGGGATTGATAACAAGACAAGGTTAAAGTCATAGTCTAATCGTCTAGACTGATAAGGCAAAA
CTGAAGCTTAAGAGGACACC

Downstream 100 bases:

>100_bases
GGATTCTGTCCTGATTAACTCCTATTAGAAGTCCTAACTTTTGGAACTTAAGCCTCTGTAAATTCGTCTTTATAGAGGCT
AATCCTGGGACAATAGTGCT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MRLSQMLFVTLREDPAEAEIPSHKLLLRAGYIRRVGSGIYAYLPLMWRVLQKVSQIVREEMDAAGAQECLLPQIQPAELW
QESGRWDTYTQAEGIMFALQDRQDRELGLGPTHEEVITAVARDMIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIM
KDAYSFSTDEADLKIIYSKMHDAYCNILQRSGLAYRAVDADSGAIGGSGSQEFMVLAEAGEDEVLYTDDGKYAANVEKAV
SRPADAQPSSFKSVEKKETPKTDTIAKLCDCLQCSPTQVVKTVLYEAVYDNGQTLLALVSLRGDQSVNEVKLQNELVQLG
DVVKGKALIALTVAEGGTKWASQPLPLGYIAPDLADSYIQKSKQVHGKFVRLVDQTAVDLKNFVTGANEVGIHQVGVNWK
TDIPLPKHVVDIRTAQVGDRAVHDPKQTLQTARGIEIGHIFQLGTKYSQALGATYTNEAGKEQPLVMGCYGVGVSRLAQA
AVEQSYDKDGIVWPVAIAPYHAIIIIPNGKDSDQVEAAEKLYSELNAAGIETLLDDRTERAGVKFKDADLIGIPYRIVTG
RSLKEGNVEMVERATHDAHQIALDQVLPTLKEYLAKAIST

Sequences:

>Translated_600_residues
MRLSQMLFVTLREDPAEAEIPSHKLLLRAGYIRRVGSGIYAYLPLMWRVLQKVSQIVREEMDAAGAQECLLPQIQPAELW
QESGRWDTYTQAEGIMFALQDRQDRELGLGPTHEEVITAVARDMIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIM
KDAYSFSTDEADLKIIYSKMHDAYCNILQRSGLAYRAVDADSGAIGGSGSQEFMVLAEAGEDEVLYTDDGKYAANVEKAV
SRPADAQPSSFKSVEKKETPKTDTIAKLCDCLQCSPTQVVKTVLYEAVYDNGQTLLALVSLRGDQSVNEVKLQNELVQLG
DVVKGKALIALTVAEGGTKWASQPLPLGYIAPDLADSYIQKSKQVHGKFVRLVDQTAVDLKNFVTGANEVGIHQVGVNWK
TDIPLPKHVVDIRTAQVGDRAVHDPKQTLQTARGIEIGHIFQLGTKYSQALGATYTNEAGKEQPLVMGCYGVGVSRLAQA
AVEQSYDKDGIVWPVAIAPYHAIIIIPNGKDSDQVEAAEKLYSELNAAGIETLLDDRTERAGVKFKDADLIGIPYRIVTG
RSLKEGNVEMVERATHDAHQIALDQVLPTLKEYLAKAIST
>Mature_600_residues
MRLSQMLFVTLREDPAEAEIPSHKLLLRAGYIRRVGSGIYAYLPLMWRVLQKVSQIVREEMDAAGAQECLLPQIQPAELW
QESGRWDTYTQAEGIMFALQDRQDRELGLGPTHEEVITAVARDMIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIM
KDAYSFSTDEADLKIIYSKMHDAYCNILQRSGLAYRAVDADSGAIGGSGSQEFMVLAEAGEDEVLYTDDGKYAANVEKAV
SRPADAQPSSFKSVEKKETPKTDTIAKLCDCLQCSPTQVVKTVLYEAVYDNGQTLLALVSLRGDQSVNEVKLQNELVQLG
DVVKGKALIALTVAEGGTKWASQPLPLGYIAPDLADSYIQKSKQVHGKFVRLVDQTAVDLKNFVTGANEVGIHQVGVNWK
TDIPLPKHVVDIRTAQVGDRAVHDPKQTLQTARGIEIGHIFQLGTKYSQALGATYTNEAGKEQPLVMGCYGVGVSRLAQA
AVEQSYDKDGIVWPVAIAPYHAIIIIPNGKDSDQVEAAEKLYSELNAAGIETLLDDRTERAGVKFKDADLIGIPYRIVTG
RSLKEGNVEMVERATHDAHQIALDQVLPTLKEYLAKAIST

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=413, Percent_Identity=32.4455205811138, Blast_Score=196, Evalue=5e-50,
Organism=Escherichia coli, GI1786392, Length=605, Percent_Identity=45.9504132231405, Blast_Score=497, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI115532348, Length=221, Percent_Identity=37.10407239819, Blast_Score=139, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6320931, Length=205, Percent_Identity=40.4878048780488, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24656200, Length=244, Percent_Identity=36.0655737704918, Blast_Score=158, Evalue=9e-39,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_ACAM1 (B0C230)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516645.1
- ProteinModelPortal:   B0C230
- SMR:   B0C230
- GeneID:   5681134
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2321
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   AMAR329726:AM1_2321-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 66372; Mature: 66372

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSQMLFVTLREDPAEAEIPSHKLLLRAGYIRRVGSGIYAYLPLMWRVLQKVSQIVREE
CCCHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
MDAAGAQECLLPQIQPAELWQESGRWDTYTQAEGIMFALQDRQDRELGLGPTHEEVITAV
HHHCCCHHHCCCCCCHHHHHHHCCCCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHHHH
ARDMIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFSTDEADLKIIYSKM
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
HDAYCNILQRSGLAYRAVDADSGAIGGSGSQEFMVLAEAGEDEVLYTDDGKYAANVEKAV
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCHHHHHHHHH
SRPADAQPSSFKSVEKKETPKTDTIAKLCDCLQCSPTQVVKTVLYEAVYDNGQTLLALVS
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEHEEE
LRGDQSVNEVKLQNELVQLGDVVKGKALIALTVAEGGTKWASQPLPLGYIAPDLADSYIQ
CCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
KSKQVHGKFVRLVDQTAVDLKNFVTGANEVGIHQVGVNWKTDIPLPKHVVDIRTAQVGDR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCH
AVHDPKQTLQTARGIEIGHIFQLGTKYSQALGATYTNEAGKEQPLVMGCYGVGVSRLAQA
HHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHCHHHHHHH
AVEQSYDKDGIVWPVAIAPYHAIIIIPNGKDSDQVEAAEKLYSELNAAGIETLLDDRTER
HHHHCCCCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
AGVKFKDADLIGIPYRIVTGRSLKEGNVEMVERATHDAHQIALDQVLPTLKEYLAKAIST
CCCEEECCCEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLSQMLFVTLREDPAEAEIPSHKLLLRAGYIRRVGSGIYAYLPLMWRVLQKVSQIVREE
CCCHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
MDAAGAQECLLPQIQPAELWQESGRWDTYTQAEGIMFALQDRQDRELGLGPTHEEVITAV
HHHCCCHHHCCCCCCHHHHHHHCCCCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHHHH
ARDMIRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFSTDEADLKIIYSKM
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
HDAYCNILQRSGLAYRAVDADSGAIGGSGSQEFMVLAEAGEDEVLYTDDGKYAANVEKAV
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCHHHHHHHHH
SRPADAQPSSFKSVEKKETPKTDTIAKLCDCLQCSPTQVVKTVLYEAVYDNGQTLLALVS
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEHEEE
LRGDQSVNEVKLQNELVQLGDVVKGKALIALTVAEGGTKWASQPLPLGYIAPDLADSYIQ
CCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
KSKQVHGKFVRLVDQTAVDLKNFVTGANEVGIHQVGVNWKTDIPLPKHVVDIRTAQVGDR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCH
AVHDPKQTLQTARGIEIGHIFQLGTKYSQALGATYTNEAGKEQPLVMGCYGVGVSRLAQA
HHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHCHHHHHHH
AVEQSYDKDGIVWPVAIAPYHAIIIIPNGKDSDQVEAAEKLYSELNAAGIETLLDDRTER
HHHHCCCCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
AGVKFKDADLIGIPYRIVTGRSLKEGNVEMVERATHDAHQIALDQVLPTLKEYLAKAIST
CCCEEECCCEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA