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The map label for this gene is hsdR [H]

Identifier: 158335392

GI number: 158335392

Start: 2246217

End: 2248571

Strand: Reverse

Name: hsdR [H]

Synonym: AM1_2237

Alternate gene names: 158335392

Gene position: 2248571-2246217 (Counterclockwise)

Preceding gene: 158335394

Following gene: 158335391

Centisome position: 34.57

GC content: 52.27

Gene sequence:

>2355_bases
ATGACCAAAAAATCTCTGAGTGAAGTGGATATTTGCGATCGCTATATTACTCCGGCGATTGATGCGGCGGGTTGGAAGAA
AAGCCAAATCCGGCGGGAATATGGCTTTACGGATGGGCAGATCATTGTGCGGGGGCAATTGACGACGAGGGGAAAACGCA
AGCGAGCAGATTATTTGCTGTTTTACCCTCAGAACCAGCCGATTGCCGTAATTGAAGCCAAGGATAACAAACATAGTTTG
GGGGCGGGAATGCAGCAGGCCCTGGGATATGCCGAGACTTTGCAGGTTCCGTTTGTGTTTTCTTCGAATGGGGATGGCTT
TTTGCTCCATGATCGCAGTGGTACTTATCCTCAGGTGGAGCAGGCGCTAGCGCTGGAGGTGTTTCCGTCACCGGATGAGC
TGTGGCAACGGTATCGTCAGAGCCAAGGGTTGTTGGGGGTGGACGAGGCGCTGCTCACCTCGCCTTATTATTTTGAGATA
GGAGGGAAGAAGCCCCGCTACTATCAGCAGTTGGCGATCAATCAGACCTTGGCGGCTATTGCCAAGGGGCAGAAGCGCTG
TCTCCTAGTGATGGCGACGGGAGCTGGAAAGACTTACACGGTTTTCAATATTATTTGGCGACTGTGGAAAAAGCGGGTGG
TGAAGCGGGTGCTGTTTTTAGCCGATCGCAATGCGTTGGTGGATCAAACCATTACCAATGATTTTCGCCCTTTTGGCCAG
GTGATGACTAAGCTGACGCGCAAACTGGTAGACGACAATGGCAAGATTGATACCTCCTATGAGGTCTATCTCGGTATTTA
CCAGGCGATTATTGGCCAGGAAGGCCAGGAGGATCTGTACAGCAAGTTCCCGGCAGATTTCTTTGATTTGATTGTGATTG
ATGAATGTCACCGGGGCAGCGCAGCGGAGGATTCTAACTGGCGACAGGTGTTGGATTATTTTACCAGCGCCACTCAGTTA
GGCTTAACCGCTACTCCTAAGGAAACTCAATACGTCTCTAATATTGATTATTTTGGTGAACCCATTTACACCTATTCCCT
GCGGCAGGGGATTGAGGATGGTTTCTTGGCTCCGTTCCGTCGGGTGCAGGTGCAGTTGGATAAGGATATAGAGGGCTGGA
TACCGGACGCCAATGAGGTGGATGATCAGGGGCAATTGATCGAGCAGCGGGAGTACAATTTGCGGGACTTTGACCGCAAT
ATAGTGTTTGACCAGCGAACCCAGGCGGTGGCGGAGTATGTGAGTGAGTTTCTGCATCAAGGCGACCCGATGCGCAAGAC
GATTGTCTTTTGTGAGGATATTGACCATGCGGAGCGGATGCGGCAGGCTTTTGTGAATGTTGCGGTGAATCGGCCCCTCG
TGGAGCAGGATCGGCGTTATGTGATGCGGGTAACGGGGGATGACAAGGAAGGGAAGGCCCAACTGGATCACTTTATCAAT
CCGAAAGAAGACTATCCGGTGATTACGACCACCTCGAAGCTGATGACGACGGGGGTGGATGCCCAAACCTGTCATGTGAT
TGTTTTGGATCGACGGATCCAGTCGATGACCGAGTTTAAGCAGATTGTGGGGCGAGGGACGCGGTTACGCCCCGACTTTG
GCAAAAACTTTTTCACCATTATTGATTTTCGCAATGCTACGGCATTGTTTAACGATGAGGATTGGGATGGGCCTCCGATT
CAGGATGAGCAGTTTGGTGTTGGTCCATCCTCGGCCCAGTCAGAGCCTAGAGATGTCACCCTTGACCCAGCCTTCGATGA
TGGTTCTGCTGAACGGGTGAAATACCAAGTGGGGCGTCAGGAATTTATGGTGGCGCGGGAGCGGGTGAGCTACTACGACA
AGGAGGGGAAACTGACGACGGAATCCCTCAAGGACTATAACCGTCGGACGATTAATGAGGCGTATCAGTCTCTGGATCGC
TTTTTGGGGAAATGGAATAAGGCGGATCGCAAGTCGGCGATCTTGGAGGAACTCCAGTCCCACGGAGTGATGGTGGAAGC
ATTAGCCAAACAGGTGGGGGCAGAGTATGACCTCTTTGATTTGATTTGCCATGTGGCCTTTGATCAGCCCCCCAAAACCA
GACGAGAGCGAGCCAACCAGGTGCGCAAGCGAGATGTGTTTACGCAATATGGGGAGACCGCGCGGGCGGTGTTGGAAGGT
CTGTTGGAGAAGTATGCCGATCAGGGGGTGGTCTCTATGGAGGATGCGAAGGTTCTGCAGTTGGAGCCGTTTGCCCAGTT
AGGGACGCCTGTGGAGTTGTTGAAACAGTTTGGGGGGAAGAAGCAATATCGGGCAGCGGTAAGTGAGTTGGCGCGGATGC
TGTACGATGATGAATCAGCTTCTCCAGGGGCGTAG

Upstream 100 bases:

>100_bases
GACCTATCAAACTGCCGAACCATAGGTGATTTACCTGGGTATGATGAGTAGGAGCGAGCTGGATGCTGTTCACGCTTAAA
ATACCTTTATTTTTTGTTTG

Downstream 100 bases:

>100_bases
ACAGCGCCTCACGCTTTAGCATCGGTTTTGAGGAATGGCTATGTCTTTATCCGCCACGATTAAGTCGATTCAAGACATCA
TGCGCAAGGATGTGGGGGTC

Product: type I restriction-modification system, R subunit

Products: NA

Alternate protein names: R.EcoEI [H]

Number of amino acids: Translated: 784; Mature: 783

Protein sequence:

>784_residues
MTKKSLSEVDICDRYITPAIDAAGWKKSQIRREYGFTDGQIIVRGQLTTRGKRKRADYLLFYPQNQPIAVIEAKDNKHSL
GAGMQQALGYAETLQVPFVFSSNGDGFLLHDRSGTYPQVEQALALEVFPSPDELWQRYRQSQGLLGVDEALLTSPYYFEI
GGKKPRYYQQLAINQTLAAIAKGQKRCLLVMATGAGKTYTVFNIIWRLWKKRVVKRVLFLADRNALVDQTITNDFRPFGQ
VMTKLTRKLVDDNGKIDTSYEVYLGIYQAIIGQEGQEDLYSKFPADFFDLIVIDECHRGSAAEDSNWRQVLDYFTSATQL
GLTATPKETQYVSNIDYFGEPIYTYSLRQGIEDGFLAPFRRVQVQLDKDIEGWIPDANEVDDQGQLIEQREYNLRDFDRN
IVFDQRTQAVAEYVSEFLHQGDPMRKTIVFCEDIDHAERMRQAFVNVAVNRPLVEQDRRYVMRVTGDDKEGKAQLDHFIN
PKEDYPVITTTSKLMTTGVDAQTCHVIVLDRRIQSMTEFKQIVGRGTRLRPDFGKNFFTIIDFRNATALFNDEDWDGPPI
QDEQFGVGPSSAQSEPRDVTLDPAFDDGSAERVKYQVGRQEFMVARERVSYYDKEGKLTTESLKDYNRRTINEAYQSLDR
FLGKWNKADRKSAILEELQSHGVMVEALAKQVGAEYDLFDLICHVAFDQPPKTRRERANQVRKRDVFTQYGETARAVLEG
LLEKYADQGVVSMEDAKVLQLEPFAQLGTPVELLKQFGGKKQYRAAVSELARMLYDDESASPGA

Sequences:

>Translated_784_residues
MTKKSLSEVDICDRYITPAIDAAGWKKSQIRREYGFTDGQIIVRGQLTTRGKRKRADYLLFYPQNQPIAVIEAKDNKHSL
GAGMQQALGYAETLQVPFVFSSNGDGFLLHDRSGTYPQVEQALALEVFPSPDELWQRYRQSQGLLGVDEALLTSPYYFEI
GGKKPRYYQQLAINQTLAAIAKGQKRCLLVMATGAGKTYTVFNIIWRLWKKRVVKRVLFLADRNALVDQTITNDFRPFGQ
VMTKLTRKLVDDNGKIDTSYEVYLGIYQAIIGQEGQEDLYSKFPADFFDLIVIDECHRGSAAEDSNWRQVLDYFTSATQL
GLTATPKETQYVSNIDYFGEPIYTYSLRQGIEDGFLAPFRRVQVQLDKDIEGWIPDANEVDDQGQLIEQREYNLRDFDRN
IVFDQRTQAVAEYVSEFLHQGDPMRKTIVFCEDIDHAERMRQAFVNVAVNRPLVEQDRRYVMRVTGDDKEGKAQLDHFIN
PKEDYPVITTTSKLMTTGVDAQTCHVIVLDRRIQSMTEFKQIVGRGTRLRPDFGKNFFTIIDFRNATALFNDEDWDGPPI
QDEQFGVGPSSAQSEPRDVTLDPAFDDGSAERVKYQVGRQEFMVARERVSYYDKEGKLTTESLKDYNRRTINEAYQSLDR
FLGKWNKADRKSAILEELQSHGVMVEALAKQVGAEYDLFDLICHVAFDQPPKTRRERANQVRKRDVFTQYGETARAVLEG
LLEKYADQGVVSMEDAKVLQLEPFAQLGTPVELLKQFGGKKQYRAAVSELARMLYDDESASPGA
>Mature_783_residues
TKKSLSEVDICDRYITPAIDAAGWKKSQIRREYGFTDGQIIVRGQLTTRGKRKRADYLLFYPQNQPIAVIEAKDNKHSLG
AGMQQALGYAETLQVPFVFSSNGDGFLLHDRSGTYPQVEQALALEVFPSPDELWQRYRQSQGLLGVDEALLTSPYYFEIG
GKKPRYYQQLAINQTLAAIAKGQKRCLLVMATGAGKTYTVFNIIWRLWKKRVVKRVLFLADRNALVDQTITNDFRPFGQV
MTKLTRKLVDDNGKIDTSYEVYLGIYQAIIGQEGQEDLYSKFPADFFDLIVIDECHRGSAAEDSNWRQVLDYFTSATQLG
LTATPKETQYVSNIDYFGEPIYTYSLRQGIEDGFLAPFRRVQVQLDKDIEGWIPDANEVDDQGQLIEQREYNLRDFDRNI
VFDQRTQAVAEYVSEFLHQGDPMRKTIVFCEDIDHAERMRQAFVNVAVNRPLVEQDRRYVMRVTGDDKEGKAQLDHFINP
KEDYPVITTTSKLMTTGVDAQTCHVIVLDRRIQSMTEFKQIVGRGTRLRPDFGKNFFTIIDFRNATALFNDEDWDGPPIQ
DEQFGVGPSSAQSEPRDVTLDPAFDDGSAERVKYQVGRQEFMVARERVSYYDKEGKLTTESLKDYNRRTINEAYQSLDRF
LGKWNKADRKSAILEELQSHGVMVEALAKQVGAEYDLFDLICHVAFDQPPKTRRERANQVRKRDVFTQYGETARAVLEGL
LEKYADQGVVSMEDAKVLQLEPFAQLGTPVELLKQFGGKKQYRAAVSELARMLYDDESASPGA

Specific function: The EcoEI enzyme recognizes 5'-GAGN(7)ATGC-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification [H]

COG id: COG4096

COG function: function code V; Type I site-specific restriction-modification system, R (restriction) subunit and related helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI226510991, Length=583, Percent_Identity=29.3310463121784, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR013670
- InterPro:   IPR007409 [H]

Pfam domain/function: PF08463 EcoEI_R_C; PF04313 HSDR_N; PF04851 ResIII [H]

EC number: =3.1.21.3 [H]

Molecular weight: Translated: 89584; Mature: 89453

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKSLSEVDICDRYITPAIDAAGWKKSQIRREYGFTDGQIIVRGQLTTRGKRKRADYLL
CCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEE
FYPQNQPIAVIEAKDNKHSLGAGMQQALGYAETLQVPFVFSSNGDGFLLHDRSGTYPQVE
EECCCCCEEEEEECCCCCHHHCCHHHHCCHHHHEEEEEEEECCCCEEEEECCCCCCHHHH
QALALEVFPSPDELWQRYRQSQGLLGVDEALLTSPYYFEIGGKKPRYYQQLAINQTLAAI
HHHEEEECCCHHHHHHHHHHCCCCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHH
AKGQKRCLLVMATGAGKTYTVFNIIWRLWKKRVVKRVLFLADRNALVDQTITNDFRPFGQ
HCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
VMTKLTRKLVDDNGKIDTSYEVYLGIYQAIIGQEGQEDLYSKFPADFFDLIVIDECHRGS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHEEEEECCCCCC
AAEDSNWRQVLDYFTSATQLGLTATPKETQYVSNIDYFGEPIYTYSLRQGIEDGFLAPFR
CCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHCCCCCEEHHHHHCCCCCCCCCHH
RVQVQLDKDIEGWIPDANEVDDQGQLIEQREYNLRDFDRNIVFDQRTQAVAEYVSEFLHQ
HHEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHC
GDPMRKTIVFCEDIDHAERMRQAFVNVAVNRPLVEQDRRYVMRVTGDDKEGKAQLDHFIN
CCCCHHEEEEECCCCHHHHHHHHHHHHHHCCCHHHCCCEEEEEEECCCCCCHHHHHHCCC
PKEDYPVITTTSKLMTTGVDAQTCHVIVLDRRIQSMTEFKQIVGRGTRLRPDFGKNFFTI
CCCCCCEEEECHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
IDFRNATALFNDEDWDGPPIQDEQFGVGPSSAQSEPRDVTLDPAFDDGSAERVKYQVGRQ
EEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
EFMVARERVSYYDKEGKLTTESLKDYNRRTINEAYQSLDRFLGKWNKADRKSAILEELQS
HHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
HGVMVEALAKQVGAEYDLFDLICHVAFDQPPKTRRERANQVRKRDVFTQYGETARAVLEG
CCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEKYADQGVVSMEDAKVLQLEPFAQLGTPVELLKQFGGKKQYRAAVSELARMLYDDESA
HHHHHHCCCCEEECCCCEEEECCHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
SPGA
CCCC
>Mature Secondary Structure 
TKKSLSEVDICDRYITPAIDAAGWKKSQIRREYGFTDGQIIVRGQLTTRGKRKRADYLL
CCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEE
FYPQNQPIAVIEAKDNKHSLGAGMQQALGYAETLQVPFVFSSNGDGFLLHDRSGTYPQVE
EECCCCCEEEEEECCCCCHHHCCHHHHCCHHHHEEEEEEEECCCCEEEEECCCCCCHHHH
QALALEVFPSPDELWQRYRQSQGLLGVDEALLTSPYYFEIGGKKPRYYQQLAINQTLAAI
HHHEEEECCCHHHHHHHHHHCCCCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHH
AKGQKRCLLVMATGAGKTYTVFNIIWRLWKKRVVKRVLFLADRNALVDQTITNDFRPFGQ
HCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
VMTKLTRKLVDDNGKIDTSYEVYLGIYQAIIGQEGQEDLYSKFPADFFDLIVIDECHRGS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHEEEEECCCCCC
AAEDSNWRQVLDYFTSATQLGLTATPKETQYVSNIDYFGEPIYTYSLRQGIEDGFLAPFR
CCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHCCCCCEEHHHHHCCCCCCCCCHH
RVQVQLDKDIEGWIPDANEVDDQGQLIEQREYNLRDFDRNIVFDQRTQAVAEYVSEFLHQ
HHEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHC
GDPMRKTIVFCEDIDHAERMRQAFVNVAVNRPLVEQDRRYVMRVTGDDKEGKAQLDHFIN
CCCCHHEEEEECCCCHHHHHHHHHHHHHHCCCHHHCCCEEEEEEECCCCCCHHHHHHCCC
PKEDYPVITTTSKLMTTGVDAQTCHVIVLDRRIQSMTEFKQIVGRGTRLRPDFGKNFFTI
CCCCCCEEEECHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
IDFRNATALFNDEDWDGPPIQDEQFGVGPSSAQSEPRDVTLDPAFDDGSAERVKYQVGRQ
EEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
EFMVARERVSYYDKEGKLTTESLKDYNRRTINEAYQSLDRFLGKWNKADRKSAILEELQS
HHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
HGVMVEALAKQVGAEYDLFDLICHVAFDQPPKTRRERANQVRKRDVFTQYGETARAVLEG
CCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEKYADQGVVSMEDAKVLQLEPFAQLGTPVELLKQFGGKKQYRAAVSELARMLYDDESA
HHHHHHCCCCEEECCCCEEEECCHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
SPGA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]