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The map label for this gene is hsdM [H]

Identifier: 158335391

GI number: 158335391

Start: 2244716

End: 2246176

Strand: Reverse

Name: hsdM [H]

Synonym: AM1_2236

Alternate gene names: 158335391

Gene position: 2246176-2244716 (Counterclockwise)

Preceding gene: 158335392

Following gene: 158335390

Centisome position: 34.54

GC content: 53.87

Gene sequence:

>1461_bases
ATGTCTTTATCCGCCACGATTAAGTCGATTCAAGACATCATGCGCAAGGATGTGGGGGTCGATGGAGATGCCCAGCGCAT
CGGACAGTTGGGCTGGATGCTGTTTTTTAAGATTTTTAGCGACCAGGACTTGGAACTGGAAGCCCTAGTGGATGACTATG
AGTCTCCGATTCCAGCGGAACTGCAGTGGTCGGCCTGGGCGGATAGTGAGCAATTGGGCAAGCAGGCGTTGACGGGAGAG
GCACTGCTGGATCTAGTGGATAATTGCTTGTTTCCGGCCCTAAAGGAGTTGGACCTAGAAGACCTAGAGGGAATTGCTCA
GGGGCGGGGCGCACTGCTGCGATCGGTGTTTGAAGATGCCTACAACTATATGAAGTCGGGCACGTTGCTGCGGCAGGTGG
TGAATAAGATCAATGACAATATCGATTTTAATGAGTCGAAGCAGCGGGACTTGTTTGGGGATATGTATGAGCAGTTGCTC
AAGGATCTTCAAGGGGCAGGCAATGCGGGCGAGTTTTATACGCCCCGTGCGGTCACCCAGTTTGCCATTGATAGGGTGAA
TCCGCAGTTGGGAGAGCGGGTGCTGGATCCGGCCTGCGGCACGGGGGGATTTTTGACCTGCGCCTTTGAGCATTTGAAGC
AACAGGTGCAGGGTTCGCAGGATTTGGAGCAGGCCAAGCAGGGGGTTTGGGGGGTAGAGAAGAAGCCTCTACCTCATTTG
CTCTGCGTGACGAATATGCTGGTGCATGGCCTGGAGGTGCCAACCAATGTGCGCCATGACAATACATTGCGCAAGCCCTT
ACGGGATTATGCACGGGCGGATCAGGTGGATGTGGTGGTGACCAATCCGCCCTTTGGGGGCATGGAGGAGGATGGTATTG
AGCGGGGTTTCCCAACGGAGTTCCGCACGCGGGAGACGGCGGATCTGTTTCTGGTGTTGGTGATGGAGCTGCTGAAGGCA
GGCGGACGGGCCGCGATTGTGTTGCCGGATGGGACGTTATTTGGGGAAGGGATTAAAACGCGGATTAAGGAAAAGCTGCT
GCGGGAGTGTAATTTACATACGATTGTGCGGTTGCCGAATGGGGTGTTTGCACCCTATACGAGCATTAAGACGAATCTGT
TGTTTTTTACCAAGGGGGAGCCGACCCAGGAGATTTGGTATTACGAGCATCCTTATCCCAAGGGGTATAAGTCCTACTCG
AAGACGAAGCCGATGCGGATTGAGGAGTTTGGGCCGGAGAAGGCGTGGTGGGAGCAGCGGGAGGAGAATGAGTTTGCCTG
GAAGGTGTCGCTGCAGGAGATTCAGGCGAATAACTACAATCTGGATGTAAAGAATCCGAATGCAGCGGGGGAGGTGCATG
AGGATGCGGCGGTATTGCTGGATAAGTATCGGTTTGCTGTGCAACAGGCGGATGAAGCGAGATCGGCTTTACAAGCGGCT
TTGCGAGATTGTTTAGAATAG

Upstream 100 bases:

>100_bases
GCGGTAAGTGAGTTGGCGCGGATGCTGTACGATGATGAATCAGCTTCTCCAGGGGCGTAGACAGCGCCTCACGCTTTAGC
ATCGGTTTTGAGGAATGGCT

Downstream 100 bases:

>100_bases
GAAAATCCTTTGAGCAAGGAGATGGCGAGATGACCACAGTGGCTGAAATTGAAGCAGCAATTCGACAGTTGCCGGAGGAT
GAGGCACGGCAGTTGTCAGC

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSLSATIKSIQDIMRKDVGVDGDAQRIGQLGWMLFFKIFSDQDLELEALVDDYESPIPAELQWSAWADSEQLGKQALTGE
ALLDLVDNCLFPALKELDLEDLEGIAQGRGALLRSVFEDAYNYMKSGTLLRQVVNKINDNIDFNESKQRDLFGDMYEQLL
KDLQGAGNAGEFYTPRAVTQFAIDRVNPQLGERVLDPACGTGGFLTCAFEHLKQQVQGSQDLEQAKQGVWGVEKKPLPHL
LCVTNMLVHGLEVPTNVRHDNTLRKPLRDYARADQVDVVVTNPPFGGMEEDGIERGFPTEFRTRETADLFLVLVMELLKA
GGRAAIVLPDGTLFGEGIKTRIKEKLLRECNLHTIVRLPNGVFAPYTSIKTNLLFFTKGEPTQEIWYYEHPYPKGYKSYS
KTKPMRIEEFGPEKAWWEQREENEFAWKVSLQEIQANNYNLDVKNPNAAGEVHEDAAVLLDKYRFAVQQADEARSALQAA
LRDCLE

Sequences:

>Translated_486_residues
MSLSATIKSIQDIMRKDVGVDGDAQRIGQLGWMLFFKIFSDQDLELEALVDDYESPIPAELQWSAWADSEQLGKQALTGE
ALLDLVDNCLFPALKELDLEDLEGIAQGRGALLRSVFEDAYNYMKSGTLLRQVVNKINDNIDFNESKQRDLFGDMYEQLL
KDLQGAGNAGEFYTPRAVTQFAIDRVNPQLGERVLDPACGTGGFLTCAFEHLKQQVQGSQDLEQAKQGVWGVEKKPLPHL
LCVTNMLVHGLEVPTNVRHDNTLRKPLRDYARADQVDVVVTNPPFGGMEEDGIERGFPTEFRTRETADLFLVLVMELLKA
GGRAAIVLPDGTLFGEGIKTRIKEKLLRECNLHTIVRLPNGVFAPYTSIKTNLLFFTKGEPTQEIWYYEHPYPKGYKSYS
KTKPMRIEEFGPEKAWWEQREENEFAWKVSLQEIQANNYNLDVKNPNAAGEVHEDAAVLLDKYRFAVQQADEARSALQAA
LRDCLE
>Mature_485_residues
SLSATIKSIQDIMRKDVGVDGDAQRIGQLGWMLFFKIFSDQDLELEALVDDYESPIPAELQWSAWADSEQLGKQALTGEA
LLDLVDNCLFPALKELDLEDLEGIAQGRGALLRSVFEDAYNYMKSGTLLRQVVNKINDNIDFNESKQRDLFGDMYEQLLK
DLQGAGNAGEFYTPRAVTQFAIDRVNPQLGERVLDPACGTGGFLTCAFEHLKQQVQGSQDLEQAKQGVWGVEKKPLPHLL
CVTNMLVHGLEVPTNVRHDNTLRKPLRDYARADQVDVVVTNPPFGGMEEDGIERGFPTEFRTRETADLFLVLVMELLKAG
GRAAIVLPDGTLFGEGIKTRIKEKLLRECNLHTIVRLPNGVFAPYTSIKTNLLFFTKGEPTQEIWYYEHPYPKGYKSYSK
TKPMRIEEFGPEKAWWEQREENEFAWKVSLQEIQANNYNLDVKNPNAAGEVHEDAAVLLDKYRFAVQQADEARSALQAAL
RDCLE

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=360, Percent_Identity=31.9444444444444, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 54857; Mature: 54726

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCC
>Mature Secondary Structure 
SLSATIKSIQDIMRKDVGVDGDAQRIGQLGWMLFFKIFSDQDLELEALVDDYESPIPAE
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
LQWSAWADSEQLGKQALTGEALLDLVDNCLFPALKELDLEDLEGIAQGRGALLRSVFEDA
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
YNYMKSGTLLRQVVNKINDNIDFNESKQRDLFGDMYEQLLKDLQGAGNAGEFYTPRAVTQ
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
FAIDRVNPQLGERVLDPACGTGGFLTCAFEHLKQQVQGSQDLEQAKQGVWGVEKKPLPHL
HHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHH
LCVTNMLVHGLEVPTNVRHDNTLRKPLRDYARADQVDVVVTNPPFGGMEEDGIERGFPTE
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCC
FRTRETADLFLVLVMELLKAGGRAAIVLPDGTLFGEGIKTRIKEKLLRECNLHTIVRLPN
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCC
GVFAPYTSIKTNLLFFTKGEPTQEIWYYEHPYPKGYKSYSKTKPMRIEEFGPEKAWWEQR
CCCCCCHHHCCCEEEEECCCCCHHEEEEECCCCCCHHHHHCCCCCCHHHCCCCHHHHHHH
EENEFAWKVSLQEIQANNYNLDVKNPNAAGEVHEDAAVLLDKYRFAVQQADEARSALQAA
CCCCEEEEEEHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDCLE
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]