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The map label for this gene is asnC [H]

Identifier: 158335177

GI number: 158335177

Start: 2009499

End: 2010890

Strand: Reverse

Name: asnC [H]

Synonym: AM1_2020

Alternate gene names: 158335177

Gene position: 2010890-2009499 (Counterclockwise)

Preceding gene: 158335181

Following gene: 158335175

Centisome position: 30.92

GC content: 45.69

Gene sequence:

>1392_bases
ATGGAAATTAGACGGATTATTGAGTTACTTCGTCATGGGACACCGGGCGAAAGCATTAAAGTGCAAGGTTGGGTTCGTAC
AAAACGAGAGCTAAAAGAGTTTGCATTCCTAGATGTGAACGATGGGTCATCGCTACAGGGACTGCAAGTGGTTGTCAACC
AAAACCTGCCAGAGTATGAGCAAACCCTCAAATTATTAAATACTGGATCCTCTGTATCTATTGAGGGCGAGTTGGTGGCA
TCTCAAGGAAAAAATCAGCGTGTTGAGCTAGTCGCCAAGGCGGTGCAAGTGTGGGGAACAGCGGATTTAGAGACCTATCC
ACTCCAGAAAAAGCGACATTCTTTTGAGTTTTTACGGACGATTGCCCATTTGAGATCGCGCACCAATACCTTGGGTTCAG
TTTTCAGAATCCGTAATGCCTGTTCCCAGGCCATTCATCAATTTTTCCAAGACCGGGACTTTATGTGGATTCACACACCT
ATCATCACGGCTAGTGACTGTGAGGGGGCAGGAGAGCAATTAGCGGTCACCAGCTTAAATCTTCAGGATGTTCCCCTATC
GGAAGATCAAACGGTTGACTACAGTCAAGACTTTTTTGGCAAACCGACCTATTTGACGGTGAGCGGACAGTTAGAAGGAG
AGATTATGGCCTCTGCCTTCTGTAATGTTTATACTTTTGGTCCGACCTTTAGAGCAGAAAATTCCAATACTTCAAGGCAC
CTAGCAGAATTTTGGATGGTGGAACCAGAGGTTGCATTCTGTGATTTACAGGGGGATATGGATCTGGCAGAAGCCTTTCT
CAAACATGTTTTTAAATCGGTATTAGAGCAGTGTCCTGAAGATTTTGAATTTTTCAATCTGCGAATTGATAAATCTGTTT
TAGAGACGGCTGACACAATTATCAACAGTGAGTTTGAGCGGATTACCTATACCGAGGCCGTGAATCAGCTAGAGAAAGCC
AGCCGCAAGTTTGAGTTCCCTGTGGAATGGGGGTTGGATTTACAGTCTGAGCATGAGCGCTATTTATCCGAAGAACTGTT
CAAAAAGCCAGTTATTGTTACGGATTACCCCAAACAGATCAAAGCGTTCTATATGCGGATGAATGATGACGATAAAACAG
TCGCCGCCATGGATGTTCTGGTCCCTAAAATTGGCGAGATTATCGGTGGCTCTCAACGAGAGGAGCGTTTGGATCTATTG
GAGCAACGAATTGTCGCTCAAGGGCTGCCCTTAGAAGATTATTGGTGGTATCTCGATCTTCGCCGTTATGGCACGGTTCC
CCATGCGGGCTTTGGGCTGGGTTTTGAACGATTGGTTCAGTTTATGACGGGGATGGCGAACATTCGAGATGTGATTCCGT
TCCCTCGTACCCCCTTGAATGCTGAGTTTTAG

Upstream 100 bases:

>100_bases
AGAAATATAGATTGATTGAAGTTTGTGCCAGTATACCGACCACAGTATTCGCAGATTAGACTGATGACAGTGTTGGTAAT
AAAAAAAGTGGCATTTCTGC

Downstream 100 bases:

>100_bases
CATAGATATTGACATCTACAAAAGCCCCGCTGATTAACTCAGCGGGGCTTTTGTAGATGTTGGGATGACTAAATTTACGG
TTTAGCGCCGTGATTCGAAA

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MEIRRIIELLRHGTPGESIKVQGWVRTKRELKEFAFLDVNDGSSLQGLQVVVNQNLPEYEQTLKLLNTGSSVSIEGELVA
SQGKNQRVELVAKAVQVWGTADLETYPLQKKRHSFEFLRTIAHLRSRTNTLGSVFRIRNACSQAIHQFFQDRDFMWIHTP
IITASDCEGAGEQLAVTSLNLQDVPLSEDQTVDYSQDFFGKPTYLTVSGQLEGEIMASAFCNVYTFGPTFRAENSNTSRH
LAEFWMVEPEVAFCDLQGDMDLAEAFLKHVFKSVLEQCPEDFEFFNLRIDKSVLETADTIINSEFERITYTEAVNQLEKA
SRKFEFPVEWGLDLQSEHERYLSEELFKKPVIVTDYPKQIKAFYMRMNDDDKTVAAMDVLVPKIGEIIGGSQREERLDLL
EQRIVAQGLPLEDYWWYLDLRRYGTVPHAGFGLGFERLVQFMTGMANIRDVIPFPRTPLNAEF

Sequences:

>Translated_463_residues
MEIRRIIELLRHGTPGESIKVQGWVRTKRELKEFAFLDVNDGSSLQGLQVVVNQNLPEYEQTLKLLNTGSSVSIEGELVA
SQGKNQRVELVAKAVQVWGTADLETYPLQKKRHSFEFLRTIAHLRSRTNTLGSVFRIRNACSQAIHQFFQDRDFMWIHTP
IITASDCEGAGEQLAVTSLNLQDVPLSEDQTVDYSQDFFGKPTYLTVSGQLEGEIMASAFCNVYTFGPTFRAENSNTSRH
LAEFWMVEPEVAFCDLQGDMDLAEAFLKHVFKSVLEQCPEDFEFFNLRIDKSVLETADTIINSEFERITYTEAVNQLEKA
SRKFEFPVEWGLDLQSEHERYLSEELFKKPVIVTDYPKQIKAFYMRMNDDDKTVAAMDVLVPKIGEIIGGSQREERLDLL
EQRIVAQGLPLEDYWWYLDLRRYGTVPHAGFGLGFERLVQFMTGMANIRDVIPFPRTPLNAEF
>Mature_463_residues
MEIRRIIELLRHGTPGESIKVQGWVRTKRELKEFAFLDVNDGSSLQGLQVVVNQNLPEYEQTLKLLNTGSSVSIEGELVA
SQGKNQRVELVAKAVQVWGTADLETYPLQKKRHSFEFLRTIAHLRSRTNTLGSVFRIRNACSQAIHQFFQDRDFMWIHTP
IITASDCEGAGEQLAVTSLNLQDVPLSEDQTVDYSQDFFGKPTYLTVSGQLEGEIMASAFCNVYTFGPTFRAENSNTSRH
LAEFWMVEPEVAFCDLQGDMDLAEAFLKHVFKSVLEQCPEDFEFFNLRIDKSVLETADTIINSEFERITYTEAVNQLEKA
SRKFEFPVEWGLDLQSEHERYLSEELFKKPVIVTDYPKQIKAFYMRMNDDDKTVAAMDVLVPKIGEIIGGSQREERLDLL
EQRIVAQGLPLEDYWWYLDLRRYGTVPHAGFGLGFERLVQFMTGMANIRDVIPFPRTPLNAEF

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI226491502, Length=447, Percent_Identity=49.4407158836689, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI4758762, Length=464, Percent_Identity=27.5862068965517, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI45439306, Length=342, Percent_Identity=28.3625730994152, Blast_Score=119, Evalue=6e-27,
Organism=Escherichia coli, GI1787161, Length=447, Percent_Identity=58.3892617449664, Blast_Score=556, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI17556372, Length=430, Percent_Identity=33.2558139534884, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI71984122, Length=451, Percent_Identity=30.1552106430155, Blast_Score=185, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17551876, Length=476, Percent_Identity=25.6302521008403, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6319872, Length=471, Percent_Identity=38.2165605095541, Blast_Score=316, Evalue=6e-87,
Organism=Saccharomyces cerevisiae, GI6321807, Length=451, Percent_Identity=28.6031042128603, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6323011, Length=344, Percent_Identity=28.7790697674419, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI19922416, Length=459, Percent_Identity=36.6013071895425, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI19921528, Length=455, Percent_Identity=29.4505494505495, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17136276, Length=411, Percent_Identity=27.007299270073, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 53074; Mature: 53074

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRRIIELLRHGTPGESIKVQGWVRTKRELKEFAFLDVNDGSSLQGLQVVVNQNLPEYE
CCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHEEEECCCCCCCCHHHEEECCCCCHHH
QTLKLLNTGSSVSIEGELVASQGKNQRVELVAKAVQVWGTADLETYPLQKKRHSFEFLRT
HHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
IAHLRSRTNTLGSVFRIRNACSQAIHQFFQDRDFMWIHTPIITASDCEGAGEQLAVTSLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCCCEEEEEECC
LQDVPLSEDQTVDYSQDFFGKPTYLTVSGQLEGEIMASAFCNVYTFGPTFRAENSNTSRH
CCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCCHHHHHHHHCEEECCCEEECCCCCCHHH
LAEFWMVEPEVAFCDLQGDMDLAEAFLKHVFKSVLEQCPEDFEFFNLRIDKSVLETADTI
HHHHHEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHH
INSEFERITYTEAVNQLEKASRKFEFPVEWGLDLQSEHERYLSEELFKKPVIVTDYPKQI
HCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHCCEEEECCHHHH
KAFYMRMNDDDKTVAAMDVLVPKIGEIIGGSQREERLDLLEQRIVAQGLPLEDYWWYLDL
HHHHHEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHEEEEH
RRYGTVPHAGFGLGFERLVQFMTGMANIRDVIPFPRTPLNAEF
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MEIRRIIELLRHGTPGESIKVQGWVRTKRELKEFAFLDVNDGSSLQGLQVVVNQNLPEYE
CCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHEEEECCCCCCCCHHHEEECCCCCHHH
QTLKLLNTGSSVSIEGELVASQGKNQRVELVAKAVQVWGTADLETYPLQKKRHSFEFLRT
HHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
IAHLRSRTNTLGSVFRIRNACSQAIHQFFQDRDFMWIHTPIITASDCEGAGEQLAVTSLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCCCEEEEEECC
LQDVPLSEDQTVDYSQDFFGKPTYLTVSGQLEGEIMASAFCNVYTFGPTFRAENSNTSRH
CCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCCHHHHHHHHCEEECCCEEECCCCCCHHH
LAEFWMVEPEVAFCDLQGDMDLAEAFLKHVFKSVLEQCPEDFEFFNLRIDKSVLETADTI
HHHHHEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHH
INSEFERITYTEAVNQLEKASRKFEFPVEWGLDLQSEHERYLSEELFKKPVIVTDYPKQI
HCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHCCEEEECCHHHH
KAFYMRMNDDDKTVAAMDVLVPKIGEIIGGSQREERLDLLEQRIVAQGLPLEDYWWYLDL
HHHHHEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHEEEEH
RRYGTVPHAGFGLGFERLVQFMTGMANIRDVIPFPRTPLNAEF
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]