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The map label for this gene is pyrB

Identifier: 158335045

GI number: 158335045

Start: 1876832

End: 1877803

Strand: Direct

Name: pyrB

Synonym: AM1_1883

Alternate gene names: 158335045

Gene position: 1876832-1877803 (Clockwise)

Preceding gene: 158335043

Following gene: 158335047

Centisome position: 28.86

GC content: 52.78

Gene sequence:

>972_bases
ATGGCTGAAACGACCTGGAATCGTCACCATGTTTTGTCTCTAGCAGACTTTAGCGCCGATGAATTGAATATCGTCTTGCA
AACGGCCAATAGTTTCAAAGATGTGCTGTATCGCAAGACCAAAAAAGTCCCCACCTTGCAGGCCCGATTAGTCACCAACC
TTTTCTTTGAACCGTCCACCCGTACCCGCAGTAGTTTTGAGCTGGCTGCCAAACGCCTCTCTGCTGATGTCCTCAACTTC
TCTCCAGGCACCTCTTCTTTGAGCAAAGGGGAGACCATTCTGGATACGGCCAAAACCTTCTTGGCGATGGGGGCAGACAT
GATGGTGATTCGCCATCAGCATTCGGGGGTTCCCCAGATGATTGCCCAAGCCATGGACGATCTGGGAGAGCAGGTCGGTG
TTCTCAACGCGGGTGATGGGTTTCATGAACATCCTTCCCAGGGATTGCTGGATCTGTTTACCCTATGCACAACCCTAGAT
CCGGATCAGCCAACTACCCATCTACTGACGGGTAAAAAAATTGCCATTGTCGGCGATATTTTGCATTCACGGGTGGCTCG
TTCGAATCTCTCTAGCCTAGTCACCTGCGGAGCAGATGTGCATTTGGCAGGGCCACCGACCCTATTGCCCAAGGAGTTTG
CAGACTATGGTGCGCAAGTCCATTGGTCCCTAGAACCGGCCCTGGAACAGGCGGATTTTGTGATGACGCTGCGATTGCAG
CATGAACGGATGAGCCAGCATTTGATTCCCAGTATTCGGGAGTATCACCAGCAGTTTGGGATTACCCGCGATCGCATCCA
GCGATGCCAACCCCAGGTCAAAGTGCTGCACCCCGGCCCCGTCAATCGAGGCGTCGAAATCAGCTCCGATCTGATGGATG
ACGAAAGCCTGAGTCTGATTCCCCATCAGGTGACCAGTGGTATTGCTGTGCGAATGGCTCTGCTTTACCTGATCAGCAAT
CGCAGCAGGTAG

Upstream 100 bases:

>100_bases
TAGAGAATGAACAACTGAGTCGTCCTAGCCAAATCCCGATATACTGCTTGCAGCAATTGGAATTGCCTAGCCGACCTTCG
TTAATTGTAGTTGGGTGCTT

Downstream 100 bases:

>100_bases
GGCAATAATTCTATCCATCCAAACTATGTCACAAAATCGCATGTAAACTTTATTAACAATAATTACAAACTTTCGATTTT
TGTGGTAATGAGGCTACGCT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MAETTWNRHHVLSLADFSADELNIVLQTANSFKDVLYRKTKKVPTLQARLVTNLFFEPSTRTRSSFELAAKRLSADVLNF
SPGTSSLSKGETILDTAKTFLAMGADMMVIRHQHSGVPQMIAQAMDDLGEQVGVLNAGDGFHEHPSQGLLDLFTLCTTLD
PDQPTTHLLTGKKIAIVGDILHSRVARSNLSSLVTCGADVHLAGPPTLLPKEFADYGAQVHWSLEPALEQADFVMTLRLQ
HERMSQHLIPSIREYHQQFGITRDRIQRCQPQVKVLHPGPVNRGVEISSDLMDDESLSLIPHQVTSGIAVRMALLYLISN
RSR

Sequences:

>Translated_323_residues
MAETTWNRHHVLSLADFSADELNIVLQTANSFKDVLYRKTKKVPTLQARLVTNLFFEPSTRTRSSFELAAKRLSADVLNF
SPGTSSLSKGETILDTAKTFLAMGADMMVIRHQHSGVPQMIAQAMDDLGEQVGVLNAGDGFHEHPSQGLLDLFTLCTTLD
PDQPTTHLLTGKKIAIVGDILHSRVARSNLSSLVTCGADVHLAGPPTLLPKEFADYGAQVHWSLEPALEQADFVMTLRLQ
HERMSQHLIPSIREYHQQFGITRDRIQRCQPQVKVLHPGPVNRGVEISSDLMDDESLSLIPHQVTSGIAVRMALLYLISN
RSR
>Mature_322_residues
AETTWNRHHVLSLADFSADELNIVLQTANSFKDVLYRKTKKVPTLQARLVTNLFFEPSTRTRSSFELAAKRLSADVLNFS
PGTSSLSKGETILDTAKTFLAMGADMMVIRHQHSGVPQMIAQAMDDLGEQVGVLNAGDGFHEHPSQGLLDLFTLCTTLDP
DQPTTHLLTGKKIAIVGDILHSRVARSNLSSLVTCGADVHLAGPPTLLPKEFADYGAQVHWSLEPALEQADFVMTLRLQH
ERMSQHLIPSIREYHQQFGITRDRIQRCQPQVKVLHPGPVNRGVEISSDLMDDESLSLIPHQVTSGIAVRMALLYLISNR
SR

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:
GO:0004070: Aspartate carbamoyltransferase
GO:0006207: Aspartate carbamoyltransferase
GO:0006221: Aspartate carbamoyltransferase
GO:0006520: Aspartate carbamoyltransferase
GO:0016597: Aspartate carbamoyltransferase
GO:0016740: Aspartate carbamoyltransferase
GO:0016743: Aspartate carbamoyltransferase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=323, Percent_Identity=31.5789473684211, Blast_Score=141, Evalue=7e-34,
Organism=Escherichia coli, GI2367364, Length=324, Percent_Identity=32.7160493827161, Blast_Score=137, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI193204318, Length=340, Percent_Identity=29.1176470588235, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322331, Length=320, Percent_Identity=28.4375, Blast_Score=115, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24642586, Length=322, Percent_Identity=29.8136645962733, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_ACAM1 (B0CED7)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516217.1
- ProteinModelPortal:   B0CED7
- SMR:   B0CED7
- GeneID:   5680699
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1883
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   AMAR329726:AM1_1883-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 35789; Mature: 35658

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETTWNRHHVLSLADFSADELNIVLQTANSFKDVLYRKTKKVPTLQARLVTNLFFEPST
CCCCCCCCCEEEEEECCCCCCEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
RTRSSFELAAKRLSADVLNFSPGTSSLSKGETILDTAKTFLAMGADMMVIRHQHSGVPQM
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHH
IAQAMDDLGEQVGVLNAGDGFHEHPSQGLLDLFTLCTTLDPDQPTTHLLTGKKIAIVGDI
HHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHH
LHSRVARSNLSSLVTCGADVHLAGPPTLLPKEFADYGAQVHWSLEPALEQADFVMTLRLQ
HHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHCCCEEEEECCHHHHHCCCCEEEHHH
HERMSQHLIPSIREYHQQFGITRDRIQRCQPQVKVLHPGPVNRGVEISSDLMDDESLSLI
HHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEECCCCCCCCCCCHHHHCCCCCCHHH
PHQVTSGIAVRMALLYLISNRSR
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AETTWNRHHVLSLADFSADELNIVLQTANSFKDVLYRKTKKVPTLQARLVTNLFFEPST
CCCCCCCCEEEEEECCCCCCEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
RTRSSFELAAKRLSADVLNFSPGTSSLSKGETILDTAKTFLAMGADMMVIRHQHSGVPQM
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHH
IAQAMDDLGEQVGVLNAGDGFHEHPSQGLLDLFTLCTTLDPDQPTTHLLTGKKIAIVGDI
HHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHH
LHSRVARSNLSSLVTCGADVHLAGPPTLLPKEFADYGAQVHWSLEPALEQADFVMTLRLQ
HHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHCCCEEEEECCHHHHHCCCCEEEHHH
HERMSQHLIPSIREYHQQFGITRDRIQRCQPQVKVLHPGPVNRGVEISSDLMDDESLSLI
HHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEECCCCCCCCCCCHHHHCCCCCCHHH
PHQVTSGIAVRMALLYLISNRSR
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA