Click here to switch to the map view.

The map label for this gene is murB

Identifier: 158334962

GI number: 158334962

Start: 1788878

End: 1789804

Strand: Reverse

Name: murB

Synonym: AM1_1799

Alternate gene names: 158334962

Gene position: 1789804-1788878 (Counterclockwise)

Preceding gene: 158334963

Following gene: 158334961

Centisome position: 27.52

GC content: 48.22

Gene sequence:

>927_bases
ATGACTCAGTCTTTACCCTCCATCTTTATTGACAATGGCAACTGCGAAATTCAGCCTTATGTGAGTTTGGCCGATATGAC
GACATTCCGCGTAGGGGGAGCAGCGGAGTGGTTTATCGCTCCCCATAATCTAAAAGAATTACAAGCTAGCTATGCTTGGG
CTAATGAGCAAGCTCTGCCCATCACCTTTTTGGGGGCAGGTTCAAACTTGCTCATTAGTGACCAGGGATTACCAGGTTTA
GTCATCTCAACGCGGTACTTGCGGCAAAGAACGTTCGATCCTGAAACTTGTCAAGTTACAGCCTATGCTGGGGAATCGTT
ACCTAAACTAGCTTGGCAAGCTGCTAAACGGGGCTGGTCGGGTTTGGAATGGGCCGTCGGTATTCCTGGAACGGTAGGCG
GAGCATTAGTGATGAATGCTGGAGCGCACGGTGGATGTACGGCAGATGTGTTAACTGAGGTTCATGCCCTAGACAAAGAT
GGAACGGTTCAGGTATTGAAGCCTGAACACATGGCCTTTCAGTATCGGTCATCTATCTTACAGCAATCTCCCAAGCCTGT
TTTGCTGGGAGTGTTCCAGCTTCATGCCAATCAATCGGCTGAACAGGTCAAAGCGACAACTCAGTCCCACTTAGACCATC
GCCTCAGCACGCAACCCTATGATTGGCCAAGTTGTGGCAGCGTGTTTCGCAATCCGCTGCCGCGAACGGCAGGTTGGCTA
ATTGAGCAATCAGGCTTGAAAGGATACTCACTAGGAGGGGCGCAAGTTGCTCAAAAGCACGCTAACTTTATCCTTAATTC
TGGTAACGCCACGGCGACTGACATCTTCAATCTGATTCATTATGTTCAGCAAAAAGTTGAGGAAAATTGGTCATTGCTGC
TCAAACCAGAGGTAAAAATGTTGGGTAAATTTCCACAAATCACATAG

Upstream 100 bases:

>100_bases
TAGTCCTATTCTTGGGAGCAGGAGATTTGAACCGCATCATTCCAGATTTGCTTACCCATTTTCAGGTCTCATCAAAACCT
TCTCCTGAGGTTGTTTTGAA

Downstream 100 bases:

>100_bases
AAAGTAGATTCCCTTTACGCCTCTTAGGGTAGACTCTCAGTATCATAGTAAACAGTTCGATTTGAGGTTCCTTAGAGGAG
CTCATCTAGAGCAAAGCAAA

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTQSLPSIFIDNGNCEIQPYVSLADMTTFRVGGAAEWFIAPHNLKELQASYAWANEQALPITFLGAGSNLLISDQGLPGL
VISTRYLRQRTFDPETCQVTAYAGESLPKLAWQAAKRGWSGLEWAVGIPGTVGGALVMNAGAHGGCTADVLTEVHALDKD
GTVQVLKPEHMAFQYRSSILQQSPKPVLLGVFQLHANQSAEQVKATTQSHLDHRLSTQPYDWPSCGSVFRNPLPRTAGWL
IEQSGLKGYSLGGAQVAQKHANFILNSGNATATDIFNLIHYVQQKVEENWSLLLKPEVKMLGKFPQIT

Sequences:

>Translated_308_residues
MTQSLPSIFIDNGNCEIQPYVSLADMTTFRVGGAAEWFIAPHNLKELQASYAWANEQALPITFLGAGSNLLISDQGLPGL
VISTRYLRQRTFDPETCQVTAYAGESLPKLAWQAAKRGWSGLEWAVGIPGTVGGALVMNAGAHGGCTADVLTEVHALDKD
GTVQVLKPEHMAFQYRSSILQQSPKPVLLGVFQLHANQSAEQVKATTQSHLDHRLSTQPYDWPSCGSVFRNPLPRTAGWL
IEQSGLKGYSLGGAQVAQKHANFILNSGNATATDIFNLIHYVQQKVEENWSLLLKPEVKMLGKFPQIT
>Mature_307_residues
TQSLPSIFIDNGNCEIQPYVSLADMTTFRVGGAAEWFIAPHNLKELQASYAWANEQALPITFLGAGSNLLISDQGLPGLV
ISTRYLRQRTFDPETCQVTAYAGESLPKLAWQAAKRGWSGLEWAVGIPGTVGGALVMNAGAHGGCTADVLTEVHALDKDG
TVQVLKPEHMAFQYRSSILQQSPKPVLLGVFQLHANQSAEQVKATTQSHLDHRLSTQPYDWPSCGSVFRNPLPRTAGWLI
EQSGLKGYSLGGAQVAQKHANFILNSGNATATDIFNLIHYVQQKVEENWSLLLKPEVKMLGKFPQIT

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=334, Percent_Identity=27.5449101796407, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_ACAM1 (B0CCD5)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516134.1
- ProteinModelPortal:   B0CCD5
- SMR:   B0CCD5
- GeneID:   5680615
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1799
- HOGENOM:   HBG686573
- OMA:   SKKHAGF
- ProtClustDB:   PRK13905
- BioCyc:   AMAR329726:AM1_1799-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 33585; Mature: 33453

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 176-176 ACT_SITE 227-227 ACT_SITE 297-297

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSLPSIFIDNGNCEIQPYVSLADMTTFRVGGAAEWFIAPHNLKELQASYAWANEQALP
CCCCCCEEEEECCCEEEEEEEEEECCEEEEECCCEEEEECCCCHHHHHHHHHCCCCCCCE
ITFLGAGSNLLISDQGLPGLVISTRYLRQRTFDPETCQVTAYAGESLPKLAWQAAKRGWS
EEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCC
GLEWAVGIPGTVGGALVMNAGAHGGCTADVLTEVHALDKDGTVQVLKPEHMAFQYRSSIL
CCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHH
QQSPKPVLLGVFQLHANQSAEQVKATTQSHLDHRLSTQPYDWPSCGSVFRNPLPRTAGWL
HCCCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCHHHE
IEQSGLKGYSLGGAQVAQKHANFILNSGNATATDIFNLIHYVQQKVEENWSLLLKPEVKM
EECCCCCCCCCCHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHH
LGKFPQIT
HCCCCCCC
>Mature Secondary Structure 
TQSLPSIFIDNGNCEIQPYVSLADMTTFRVGGAAEWFIAPHNLKELQASYAWANEQALP
CCCCCEEEEECCCEEEEEEEEEECCEEEEECCCEEEEECCCCHHHHHHHHHCCCCCCCE
ITFLGAGSNLLISDQGLPGLVISTRYLRQRTFDPETCQVTAYAGESLPKLAWQAAKRGWS
EEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCC
GLEWAVGIPGTVGGALVMNAGAHGGCTADVLTEVHALDKDGTVQVLKPEHMAFQYRSSIL
CCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHH
QQSPKPVLLGVFQLHANQSAEQVKATTQSHLDHRLSTQPYDWPSCGSVFRNPLPRTAGWL
HCCCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCHHHE
IEQSGLKGYSLGGAQVAQKHANFILNSGNATATDIFNLIHYVQQKVEENWSLLLKPEVKM
EECCCCCCCCCCHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHH
LGKFPQIT
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA