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The map label for this gene is pbpG [H]

Identifier: 158334907

GI number: 158334907

Start: 1732999

End: 1735242

Strand: Direct

Name: pbpG [H]

Synonym: AM1_1744

Alternate gene names: 158334907

Gene position: 1732999-1735242 (Clockwise)

Preceding gene: 158334900

Following gene: 158334908

Centisome position: 26.65

GC content: 51.78

Gene sequence:

>2244_bases
ATGACATCTCCTAAACCTTCTCCGTCAAAAACCTTTCTAGGGAATGTGACGCAGGTGGTTCGTACCATTCAAGCGAAAGC
AGATCTGTCAAAGCTTACCCTGAAGCGGAATGCTCGGGTGCCAGAGCTAAAAATTAATTCCGGTGAAGGGGAACCCCAAA
GCTATCCCCTGATTAGCGATCGCTATATTATTGGCCGCAGTGCCCGCACCTGTGACATTGTGATTCGCAATCCCATTGTC
AGCCAGGTACATGCCTCGATTCAGCGGGATGGCAAACGCCGCACCTTTTTGATTCGGGATGAGCAATCCACCAACGGCAT
TTTCCGCCGCAAACGTCGAGTAGACTCCCATGCCTTGCGCCATAAAGATGTCCTCACCCTCGGCCCCGCCGCTCTAGAAG
CTGCGGTCAGTGTCGAATATATCGATCCACCGCCCTGGTACGTGCGAGGGATGCGCTATCTTCTCTATGGCACTGCAGGC
GTCGTGGGCTTAACCCTCCTCGTGGTCGCGGCAGAATGGCAGAAATTCACGGTTCGTCCCTTACCCACCACTAACCAAGG
GCCTATCGTCGTTCTATCCCGAGACCAAACGCCTTTGGCACGCCGTCGGGATACGGTTCATGGGGAGTTGAAAAGTCTGG
ATGATTTTTCCCCCCATATTGTGAATGCCTTACTAGCCTCTGAGGACAGTCGCTTCTATTGGCATATGGGCGTCGACCCG
ATTGGGATTGTCCGGGCAGTGATTACTAACGTGGCTGGGGGCAGTGTTAGGGAAGGGGCCAGTACGATTACCCAACAGCT
TGCTCGCAGCGTTTTTCGTAGCTACGTCGGCAGTGAAGATACCCTAGACCGCAAATTCCGCGAGGCCGTCGTTGCGCTTA
AATTAGAGACCTACTACAGCAAAGACTTCTTATTGCTGACCTACTTAAATAAGGTCTATTTAGGCATCTATGCCAATGGG
TTTGAAGATGCAGCTCGCTTTTATTTTGATAAGTCTGCCAAAGACTTAAGCATTGCCGAATCTGCCACCCTCGTGGGCAT
TTTACCTGCCCCTAATGCGTTTAACCCTGTCCAGGACTACAATGCGGCCCTAGAATTTCGCAATCGAGTCATTACCCGCA
TGGCTCAGCAAGGTCGAATTAGCCAAGAAGAAGCTAATCGCGCCCGTCGTTCTCGCATTGAAATTAGCCCCAAAGCTCGG
GAAGAACTGCAAAGCGTCAAGGCCCCTTACTATTACAGCTATGTCTTTGACGAGCTAGAGGAATTATTAGGGGCCAGTCT
GGCTCAAGAAGGCAATTTTATTGTCACGACTAACTTAGATCTGGCGGTTCAAAACACGGCAGAACAAACCCTGTCCAATG
CCGTCGCCACCTCTGGATCAACCTTTGGCTATTCCCAAGGGGCGTTACTCACCGTGGATTATGAAGCGGGGGAAATTATT
GCATTAGTGGGCGGCACCGACTTTAGTAAAAGTCAGTTTAACCGCGTCACCCAAGCCCAGCGACAACCCGGCTCGACTTT
TAAACTATTCGGCTATACGGCAGCCGTAGATGCCAATATCTCCCCTAATAAAACCTATTCCTGCTCTCCGTTGACTTGGC
AGGGACAAACCTTTACCGGCTGCCAGAATGGCGGCAGCGGCAATATTGATATGTTTCGAGGATTTGCCCTATCCGAAAAT
GTTGTGGCTTTGCGTGTCGCCCAAGATGTTGGTATTCCCCGAGTGGTCCAAACCGCTCGCCAATTGGGAATTACCTCTCC
CTTGCAGGCGGTGCCAGCCTTAGTACTAGGGCAAAGTGAAGTAACGCCCTTGGAGCTGACGCGGGCTTTTGCTATTGTGG
CCAATCGGGGTCGTCGCCATACCCCCCGAGCCATCCGCCAGGTACTCGATGCCGGAGATTGCAAAGATTCCCGTTCTATT
TCTACCTGCCGAGTCATTTATGACGGCACCCAAAATGCCAGCACTCAAGTGCTCGATCCTGGGGTTGCTGTGGTGATGCA
AGATATGATGCGCCAAGTGGTTCAATCCGGCACAGGGCGAGGCGCTGCGGTTGCCTCTGGGGCTGCGGGAAAAACAGGTA
CCACTAATTTAGGTCGAGATTTATGGTTTGTGGGTTACGTGCCTCGCCGCAACTTATTGACAGGAATTTGGCTCGGCAAT
GACGATAATAGCCCTACATCTGGCAGTAGTGCCCAAGCCGCTGCTGTATGGGGAGATTATATGCGGCAAATCATCAATCA
ATGA

Upstream 100 bases:

>100_bases
CCCGCTGGGCCAAGCACAGCTTATACGATCGAACCTGACGCCCCCCTGTACGACGCATTGGTACTCACCGAGCCATCAAC
CTGACGATTGAGATTTGCCC

Downstream 100 bases:

>100_bases
CAGGGAGTAATGCTGAAGGTTATATTAAGATATGTTATCCATTCTTACAAAAAACCTTAAGATTAAGCCGTAATCCCCTT
CACAACCCTTTTGGAAGACC

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 747; Mature: 746

Protein sequence:

>747_residues
MTSPKPSPSKTFLGNVTQVVRTIQAKADLSKLTLKRNARVPELKINSGEGEPQSYPLISDRYIIGRSARTCDIVIRNPIV
SQVHASIQRDGKRRTFLIRDEQSTNGIFRRKRRVDSHALRHKDVLTLGPAALEAAVSVEYIDPPPWYVRGMRYLLYGTAG
VVGLTLLVVAAEWQKFTVRPLPTTNQGPIVVLSRDQTPLARRRDTVHGELKSLDDFSPHIVNALLASEDSRFYWHMGVDP
IGIVRAVITNVAGGSVREGASTITQQLARSVFRSYVGSEDTLDRKFREAVVALKLETYYSKDFLLLTYLNKVYLGIYANG
FEDAARFYFDKSAKDLSIAESATLVGILPAPNAFNPVQDYNAALEFRNRVITRMAQQGRISQEEANRARRSRIEISPKAR
EELQSVKAPYYYSYVFDELEELLGASLAQEGNFIVTTNLDLAVQNTAEQTLSNAVATSGSTFGYSQGALLTVDYEAGEII
ALVGGTDFSKSQFNRVTQAQRQPGSTFKLFGYTAAVDANISPNKTYSCSPLTWQGQTFTGCQNGGSGNIDMFRGFALSEN
VVALRVAQDVGIPRVVQTARQLGITSPLQAVPALVLGQSEVTPLELTRAFAIVANRGRRHTPRAIRQVLDAGDCKDSRSI
STCRVIYDGTQNASTQVLDPGVAVVMQDMMRQVVQSGTGRGAAVASGAAGKTGTTNLGRDLWFVGYVPRRNLLTGIWLGN
DDNSPTSGSSAQAAAVWGDYMRQIINQ

Sequences:

>Translated_747_residues
MTSPKPSPSKTFLGNVTQVVRTIQAKADLSKLTLKRNARVPELKINSGEGEPQSYPLISDRYIIGRSARTCDIVIRNPIV
SQVHASIQRDGKRRTFLIRDEQSTNGIFRRKRRVDSHALRHKDVLTLGPAALEAAVSVEYIDPPPWYVRGMRYLLYGTAG
VVGLTLLVVAAEWQKFTVRPLPTTNQGPIVVLSRDQTPLARRRDTVHGELKSLDDFSPHIVNALLASEDSRFYWHMGVDP
IGIVRAVITNVAGGSVREGASTITQQLARSVFRSYVGSEDTLDRKFREAVVALKLETYYSKDFLLLTYLNKVYLGIYANG
FEDAARFYFDKSAKDLSIAESATLVGILPAPNAFNPVQDYNAALEFRNRVITRMAQQGRISQEEANRARRSRIEISPKAR
EELQSVKAPYYYSYVFDELEELLGASLAQEGNFIVTTNLDLAVQNTAEQTLSNAVATSGSTFGYSQGALLTVDYEAGEII
ALVGGTDFSKSQFNRVTQAQRQPGSTFKLFGYTAAVDANISPNKTYSCSPLTWQGQTFTGCQNGGSGNIDMFRGFALSEN
VVALRVAQDVGIPRVVQTARQLGITSPLQAVPALVLGQSEVTPLELTRAFAIVANRGRRHTPRAIRQVLDAGDCKDSRSI
STCRVIYDGTQNASTQVLDPGVAVVMQDMMRQVVQSGTGRGAAVASGAAGKTGTTNLGRDLWFVGYVPRRNLLTGIWLGN
DDNSPTSGSSAQAAAVWGDYMRQIINQ
>Mature_746_residues
TSPKPSPSKTFLGNVTQVVRTIQAKADLSKLTLKRNARVPELKINSGEGEPQSYPLISDRYIIGRSARTCDIVIRNPIVS
QVHASIQRDGKRRTFLIRDEQSTNGIFRRKRRVDSHALRHKDVLTLGPAALEAAVSVEYIDPPPWYVRGMRYLLYGTAGV
VGLTLLVVAAEWQKFTVRPLPTTNQGPIVVLSRDQTPLARRRDTVHGELKSLDDFSPHIVNALLASEDSRFYWHMGVDPI
GIVRAVITNVAGGSVREGASTITQQLARSVFRSYVGSEDTLDRKFREAVVALKLETYYSKDFLLLTYLNKVYLGIYANGF
EDAARFYFDKSAKDLSIAESATLVGILPAPNAFNPVQDYNAALEFRNRVITRMAQQGRISQEEANRARRSRIEISPKARE
ELQSVKAPYYYSYVFDELEELLGASLAQEGNFIVTTNLDLAVQNTAEQTLSNAVATSGSTFGYSQGALLTVDYEAGEIIA
LVGGTDFSKSQFNRVTQAQRQPGSTFKLFGYTAAVDANISPNKTYSCSPLTWQGQTFTGCQNGGSGNIDMFRGFALSENV
VALRVAQDVGIPRVVQTARQLGITSPLQAVPALVLGQSEVTPLELTRAFAIVANRGRRHTPRAIRQVLDAGDCKDSRSIS
TCRVIYDGTQNASTQVLDPGVAVVMQDMMRQVVQSGTGRGAAVASGAAGKTGTTNLGRDLWFVGYVPRRNLLTGIWLGND
DNSPTSGSSAQAAAVWGDYMRQIINQ

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=557, Percent_Identity=32.3159784560144, Blast_Score=205, Evalue=7e-54,
Organism=Escherichia coli, GI87082258, Length=283, Percent_Identity=38.1625441696113, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1788867, Length=582, Percent_Identity=28.1786941580756, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789601, Length=200, Percent_Identity=34, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 81829; Mature: 81697

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50006 FHA_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPKPSPSKTFLGNVTQVVRTIQAKADLSKLTLKRNARVPELKINSGEGEPQSYPLISD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEECC
RYIIGRSARTCDIVIRNPIVSQVHASIQRDGKRRTFLIRDEQSTNGIFRRKRRVDSHALR
CEEECCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
HKDVLTLGPAALEAAVSVEYIDPPPWYVRGMRYLLYGTAGVVGLTLLVVAAEWQKFTVRP
HCCCEEECHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
LPTTNQGPIVVLSRDQTPLARRRDTVHGELKSLDDFSPHIVNALLASEDSRFYWHMGVDP
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCH
IGIVRAVITNVAGGSVREGASTITQQLARSVFRSYVGSEDTLDRKFREAVVALKLETYYS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEHHHC
KDFLLLTYLNKVYLGIYANGFEDAARFYFDKSAKDLSIAESATLVGILPAPNAFNPVQDY
CCCHHHHHHHHHHEEEEECCHHHHHHHHHCCCCCCCEEECCCEEEEEECCCCCCCCHHHH
NAALEFRNRVITRMAQQGRISQEEANRARRSRIEISPKAREELQSVKAPYYYSYVFDELE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
ELLGASLAQEGNFIVTTNLDLAVQNTAEQTLSNAVATSGSTFGYSQGALLTVDYEAGEII
HHHHHHHHCCCCEEEEECCCEEECCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCEE
ALVGGTDFSKSQFNRVTQAQRQPGSTFKLFGYTAAVDANISPNKTYSCSPLTWQGQTFTG
EEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCEECCCEECC
CQNGGSGNIDMFRGFALSENVVALRVAQDVGIPRVVQTARQLGITSPLQAVPALVLGQSE
CCCCCCCCHHHEECEECCCCEEEEEEHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCC
VTPLELTRAFAIVANRGRRHTPRAIRQVLDAGDCKDSRSISTCRVIYDGTQNASTQVLDP
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEECCC
GVAVVMQDMMRQVVQSGTGRGAAVASGAAGKTGTTNLGRDLWFVGYVPRRNLLTGIWLGN
HHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECC
DDNSPTSGSSAQAAAVWGDYMRQIINQ
CCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSPKPSPSKTFLGNVTQVVRTIQAKADLSKLTLKRNARVPELKINSGEGEPQSYPLISD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEECC
RYIIGRSARTCDIVIRNPIVSQVHASIQRDGKRRTFLIRDEQSTNGIFRRKRRVDSHALR
CEEECCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
HKDVLTLGPAALEAAVSVEYIDPPPWYVRGMRYLLYGTAGVVGLTLLVVAAEWQKFTVRP
HCCCEEECHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
LPTTNQGPIVVLSRDQTPLARRRDTVHGELKSLDDFSPHIVNALLASEDSRFYWHMGVDP
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCH
IGIVRAVITNVAGGSVREGASTITQQLARSVFRSYVGSEDTLDRKFREAVVALKLETYYS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEHHHC
KDFLLLTYLNKVYLGIYANGFEDAARFYFDKSAKDLSIAESATLVGILPAPNAFNPVQDY
CCCHHHHHHHHHHEEEEECCHHHHHHHHHCCCCCCCEEECCCEEEEEECCCCCCCCHHHH
NAALEFRNRVITRMAQQGRISQEEANRARRSRIEISPKAREELQSVKAPYYYSYVFDELE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
ELLGASLAQEGNFIVTTNLDLAVQNTAEQTLSNAVATSGSTFGYSQGALLTVDYEAGEII
HHHHHHHHCCCCEEEEECCCEEECCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCEE
ALVGGTDFSKSQFNRVTQAQRQPGSTFKLFGYTAAVDANISPNKTYSCSPLTWQGQTFTG
EEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCEECCCEECC
CQNGGSGNIDMFRGFALSENVVALRVAQDVGIPRVVQTARQLGITSPLQAVPALVLGQSE
CCCCCCCCHHHEECEECCCCEEEEEEHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCC
VTPLELTRAFAIVANRGRRHTPRAIRQVLDAGDCKDSRSISTCRVIYDGTQNASTQVLDP
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEECCC
GVAVVMQDMMRQVVQSGTGRGAAVASGAAGKTGTTNLGRDLWFVGYVPRRNLLTGIWLGN
HHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECC
DDNSPTSGSSAQAAAVWGDYMRQIINQ
CCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]