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The map label for this gene is trmE

Identifier: 158334891

GI number: 158334891

Start: 1713974

End: 1715341

Strand: Reverse

Name: trmE

Synonym: AM1_1728

Alternate gene names: 158334891

Gene position: 1715341-1713974 (Counterclockwise)

Preceding gene: 158334897

Following gene: 158334890

Centisome position: 26.37

GC content: 51.24

Gene sequence:

>1368_bases
ATGAAGCAAACTACGATCGCAGCGATCGCAACCGCTATTGTTCCCCAACAAGGCAGTATTGGTATCGTTCGCCTATCGGG
TGTAGAAGCAGTATCGATTGCCAAGCAGCTCTTTCAGACGCCGGGAAAGCAGCAGTGGGAGAGTCATCGGGTTCTTTATG
GTTATATTCAACAGCCTTTAACGCAGCAGATTATTGATGAAGGTTTGTTACTGCTGATGCTGGCGCCTCGCTCCTATACC
CGTGAAGATGTGGTGGAATTTCATTGCCATGGGGGCATGATTGCTGTGCAGCAGGTGCTAGAGGCCTGCTTGCAAGCAGG
TGCTGAGCTTGCACAGCCTGGAGAATTTACCCTCAGAGCTTTTTTGAATGGCCGTTTGGACTTAACCCAGGCGGAAGGGG
TAGCCGATCTGGTGGGAGCTCGTTCCCCCCAAGCGGCGCAAGCCGCCTTAGCGGGCGTGCAGGGAAAGCTGGCATCCCCA
ATTCGGGAACTGCGGCAGCGCTGTCTGGATACCTTGGCAGAAGTGGAGGCTAGGGTCGATTTTGAGGATGATTTACCACC
GTTGGATGAAGCGGGTGTCCAAGCGGAATTGCAAGACATTCATGCCACTCTTCAAGCGATTTTAGCAACGGCAGACCAAG
GCGAATTATTGCGAAATGGTCTGACAGTGGCGATTATAGGTCGTCCGAATGTGGGCAAGTCGAGCTTGTTAAATGCGTGG
TGTCGATGCGATCGCGCCATCGTTACGGACCTCCCTGGCACCACTCGGGATGTGGTGGAATCCCAGTTAGTGGTGGGAGG
CATTCCCATTCAAGTTTTAGATACCGCTGGAATTCGAGAGACTGAGGATCAGGTTGAACAAATTGGCGTCACGCGATCGC
ATCAAGCAGCTCAATCCGCTGACTTGGTTTTGCTCACCATCGATGCCTCTGTTGGATGGACCTCTGACGATCAGCAGCTC
TATCAGGCGTTTCAAGATCTACCGCTGATTTTGATTGTGAATAAAGTGGATTTAGTTCCCCAAGAGCAGGTGGTTTACCC
TGAAGCCATTGCTCAGGTTGTATCTACCATTGCCGCCCAAAACCAGGGCATCTCTGAATTGGAAACGGCAATATTGGAAA
CGGTGCAAACCCAGTCTCTGAAAGCGGCTAATTTAGATTGGGCAATTAATCAGCGACAGGCTGCTGCTCTCCAAAAAGCT
CAAGCCGCTTTAGAGCATGTCCAAGGTGCGATCGCAGATCAGCTCCCCCTTGATTTTTGGACCATAGACCTGAGAGGGGC
AATTCAGGCCTTAGGCGAAATCACGGGAGAAGACATCACAGAATCAGTGCTCGACCGCATATTTAGTCGCTTTTGTATTG
GCAAATAA

Upstream 100 bases:

>100_bases
GGTTTGGGGACGGAGGATCGATAGAGACGGGACAATGCCAAAGGTCTCACCATAAACTGGTTGACATTGCTCATATAAAC
AGACATCTCCCGGACCGCTT

Downstream 100 bases:

>100_bases
GTCTTAATTTAAATACTTTTTAAAACTTCACATATCGCGTTTGTTGTGATGTCCATCAATGATTTGTTCCAAAGGTAGGA
AATTCTGGGAATTCTAACCA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKQTTIAAIATAIVPQQGSIGIVRLSGVEAVSIAKQLFQTPGKQQWESHRVLYGYIQQPLTQQIIDEGLLLLMLAPRSYT
REDVVEFHCHGGMIAVQQVLEACLQAGAELAQPGEFTLRAFLNGRLDLTQAEGVADLVGARSPQAAQAALAGVQGKLASP
IRELRQRCLDTLAEVEARVDFEDDLPPLDEAGVQAELQDIHATLQAILATADQGELLRNGLTVAIIGRPNVGKSSLLNAW
CRCDRAIVTDLPGTTRDVVESQLVVGGIPIQVLDTAGIRETEDQVEQIGVTRSHQAAQSADLVLLTIDASVGWTSDDQQL
YQAFQDLPLILIVNKVDLVPQEQVVYPEAIAQVVSTIAAQNQGISELETAILETVQTQSLKAANLDWAINQRQAAALQKA
QAALEHVQGAIADQLPLDFWTIDLRGAIQALGEITGEDITESVLDRIFSRFCIGK

Sequences:

>Translated_455_residues
MKQTTIAAIATAIVPQQGSIGIVRLSGVEAVSIAKQLFQTPGKQQWESHRVLYGYIQQPLTQQIIDEGLLLLMLAPRSYT
REDVVEFHCHGGMIAVQQVLEACLQAGAELAQPGEFTLRAFLNGRLDLTQAEGVADLVGARSPQAAQAALAGVQGKLASP
IRELRQRCLDTLAEVEARVDFEDDLPPLDEAGVQAELQDIHATLQAILATADQGELLRNGLTVAIIGRPNVGKSSLLNAW
CRCDRAIVTDLPGTTRDVVESQLVVGGIPIQVLDTAGIRETEDQVEQIGVTRSHQAAQSADLVLLTIDASVGWTSDDQQL
YQAFQDLPLILIVNKVDLVPQEQVVYPEAIAQVVSTIAAQNQGISELETAILETVQTQSLKAANLDWAINQRQAAALQKA
QAALEHVQGAIADQLPLDFWTIDLRGAIQALGEITGEDITESVLDRIFSRFCIGK
>Mature_455_residues
MKQTTIAAIATAIVPQQGSIGIVRLSGVEAVSIAKQLFQTPGKQQWESHRVLYGYIQQPLTQQIIDEGLLLLMLAPRSYT
REDVVEFHCHGGMIAVQQVLEACLQAGAELAQPGEFTLRAFLNGRLDLTQAEGVADLVGARSPQAAQAALAGVQGKLASP
IRELRQRCLDTLAEVEARVDFEDDLPPLDEAGVQAELQDIHATLQAILATADQGELLRNGLTVAIIGRPNVGKSSLLNAW
CRCDRAIVTDLPGTTRDVVESQLVVGGIPIQVLDTAGIRETEDQVEQIGVTRSHQAAQSADLVLLTIDASVGWTSDDQQL
YQAFQDLPLILIVNKVDLVPQEQVVYPEAIAQVVSTIAAQNQGISELETAILETVQTQSLKAANLDWAINQRQAAALQKA
QAALEHVQGAIADQLPLDFWTIDLRGAIQALGEITGEDITESVLDRIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=463, Percent_Identity=33.9092872570194, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI193082993, Length=452, Percent_Identity=34.2920353982301, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI193082988, Length=493, Percent_Identity=31.6430020283976, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI2367268, Length=462, Percent_Identity=36.1471861471861, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI87082120, Length=156, Percent_Identity=36.5384615384615, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=466, Percent_Identity=30.4721030042918, Blast_Score=187, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=515, Percent_Identity=28.9320388349515, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI45550813, Length=481, Percent_Identity=31.3929313929314, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_ACAM1 (B0CBB0)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516063.1
- ProteinModelPortal:   B0CBB0
- SMR:   B0CBB0
- GeneID:   5680544
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1728
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   AMAR329726:AM1_1728-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49100; Mature: 49100

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: BINDING 24-24 BINDING 86-86 BINDING 125-125 BINDING 455-455

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTTIAAIATAIVPQQGSIGIVRLSGVEAVSIAKQLFQTPGKQQWESHRVLYGYIQQPL
CCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHH
TQQIIDEGLLLLMLAPRSYTREDVVEFHCHGGMIAVQQVLEACLQAGAELAQPGEFTLRA
HHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCHHHHHH
FLNGRLDLTQAEGVADLVGARSPQAAQAALAGVQGKLASPIRELRQRCLDTLAEVEARVD
HHCCCEEEHHHCCHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
FEDDLPPLDEAGVQAELQDIHATLQAILATADQGELLRNGLTVAIIGRPNVGKSSLLNAW
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCHHHHHHHH
CRCDRAIVTDLPGTTRDVVESQLVVGGIPIQVLDTAGIRETEDQVEQIGVTRSHQAAQSA
HHCCCHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHCCC
DLVLLTIDASVGWTSDDQQLYQAFQDLPLILIVNKVDLVPQEQVVYPEAIAQVVSTIAAQ
CEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHCCCHHHHHHHHHHHHHC
NQGISELETAILETVQTQSLKAANLDWAINQRQAAALQKAQAALEHVQGAIADQLPLDFW
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
TIDLRGAIQALGEITGEDITESVLDRIFSRFCIGK
EEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKQTTIAAIATAIVPQQGSIGIVRLSGVEAVSIAKQLFQTPGKQQWESHRVLYGYIQQPL
CCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHH
TQQIIDEGLLLLMLAPRSYTREDVVEFHCHGGMIAVQQVLEACLQAGAELAQPGEFTLRA
HHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCHHHHHH
FLNGRLDLTQAEGVADLVGARSPQAAQAALAGVQGKLASPIRELRQRCLDTLAEVEARVD
HHCCCEEEHHHCCHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
FEDDLPPLDEAGVQAELQDIHATLQAILATADQGELLRNGLTVAIIGRPNVGKSSLLNAW
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCHHHHHHHH
CRCDRAIVTDLPGTTRDVVESQLVVGGIPIQVLDTAGIRETEDQVEQIGVTRSHQAAQSA
HHCCCHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHCCC
DLVLLTIDASVGWTSDDQQLYQAFQDLPLILIVNKVDLVPQEQVVYPEAIAQVVSTIAAQ
CEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHCCCHHHHHHHHHHHHHC
NQGISELETAILETVQTQSLKAANLDWAINQRQAAALQKAQAALEHVQGAIADQLPLDFW
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
TIDLRGAIQALGEITGEDITESVLDRIFSRFCIGK
EEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA