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The map label for this gene is rne [H]

Identifier: 158334868

GI number: 158334868

Start: 1687347

End: 1689371

Strand: Direct

Name: rne [H]

Synonym: AM1_1705

Alternate gene names: 158334868

Gene position: 1687347-1689371 (Clockwise)

Preceding gene: 158334867

Following gene: 158334870

Centisome position: 25.94

GC content: 50.96

Gene sequence:

>2025_bases
ATGCCCAAGCAAATTATCATTGCCGAGCAGCATCGGATCGCTGCTGTATTTTCAGAAGATCAAATCCAAGAACTCATCGT
TGCCACTGGCAGTCATCAGGTAGGAGACATCTATCTAGGTATCGTTGAGAATGTATTACCTGGCATTGATGCTGCCTTTG
TTAACATTGGCGATGCTGAACGCAATGGATTTATTCACGTCAGCGACTTAGGTCCGGTGCGATTAAAGCGCAATTCCGGT
GCCATTACCGAGCTGTTAGCCCCCCAACAAAAAGTATTAGTCCAAATTATGAAGGAGCCTACAGGGACTAAAGGTCCACG
GCTCACAGGAAATATCTCTTTACCCGGTCGCTACCTAGTCCTTATGCCTTCAGGGCGAGGGGTCAACCTTTCTCGTCGCA
TCCGTGCAGAAGACGAACGAAGTCGTTTACGTGCCCTCGGTATTTTAATTAAGCCTGCGGGTATGGGCCTGTTGGTTCGC
ACGGAAGCCGAAGGGGTCGCCGAAGAAGCCATCATTGAAGATTTGGAACTCCTGCAACGGCAATGGGAAAGTGTCCAGCA
GCAAGCCGCTTCGACTCGAGCTCCTGCCCTACTCGATCGGGATACAGACTTTATCCAAAGAGTTCTCCGGGACGTCTATA
GCAGTAACGTCAATCGGATTGTAGTGGACTCTACTCCAGGGCTCAAACGGGTCAAACAGCACCTGGTGAACTGGAACAAT
ACCAACAAATTACCCCAGGGGGTGCTGGTGGATCAGCATCGTGAATCCACTTCCATCCTGGAATATTTCCGCGTTAATGC
GGCCATCCGAGAAGCCTTAAAGCCCAGGGTTGATCTACCGTCTGGTGGATATATCATTATCGAGAAAACGGAAGCCCTCA
CCGTTGTTGACGTCAACTCTGGCTCTTTCACTCGCTCCGCTACCGCCCGAGAGACTGTTTTGTGGACCAACTGCGAGGCT
GCTGCAGAAATCGCTCGTCAACTGCGATTACGGAATATCGCTGGTGTGATTATTATCGACTTCATCGATATGGACTCTCG
CCGCGATCAGCTACAAGTCTTAGAACATTTTAATAAAGCCCTCAAAGCGGACAAAGCTCGCCCTCAAATTGCCCAACTAA
CCGAATTAGGCTTAGTCGAGCTGACTCGCAAACGACAGGGACAAAATATTTACGAGCTATTTGGCACGCCTTGCCCGGCC
TGCAGTGGTCTTGGGCATCTGGTCCATCTGCCTGGTGAAGCTGCGCCGACGGTCGGTGAAGTGGAATGGTCTGAGCCCTC
CAAACCTTCACCCACCAGTACATCCGGTGTTCCTCTACCCGGCATCTCGGCCAACTTAAATGATCAGATCCATCTCGATC
CATCGGATGACCTACAAGAGCTTGATTTAACGAACCATCCCAGCTACCAAGAACGAGGCAATCGTCGACGCCGGCGCCGG
TCTCGATTAGGGGATAATTTGTCCCGTGAATCCCTTAGTCCTCGTTTAGTTCGTAATGGGTCTGCCTCAGATTCTAAACC
CGTCAAAAGCACGAATGATACCAATGGCTCAACCTCTAGCAAGAGCGGAGGAAATGAACGATCTCGGGGTCGCTCCAGCA
CTCGTCAACGTCAAGATACGCCTCCAGAAGTCATCTCGGTGACCATGACTCCTGAAGAACAAGATATCTACGCTTTTATG
GGCATTTCGCCGACGGTCTTGTTCCCAGGAGAGATTAAAAAGCCTAAATCAGCCATTGTGGAAATCCTCGCACCAGGGGA
GACCTCATCTACGCCTAATACAACAGCAACCAAAGTTATCACCAAAGAACTCGTTGTCGCCAAAGACACCAAAGCCGATG
CGGATGATATTGACGCAACCAAACCGGAAGAGGCTCAAGCTGAACCCGTCTTGGTTGCCACACCTGAACCGACCGAAGAA
AAACCAGCTGAACCCGCTCGGACGTCCACTCGTCGCCGCCGTCGCCGCTCTTCGGCCTCATCGGCCAAGCAGCTTTCCTT
AGACCCGAATGAAAGTTCTGAATAA

Upstream 100 bases:

>100_bases
ACTCTTGAACGTCCAAGCCCCACAATCCCTTTTGTTATTACGAGTTAAGCCCAGACATGAAACGTGTTGTCTGCTGGCTC
AGTTTCTTTGAGGAATTTGA

Downstream 100 bases:

>100_bases
CGGTTTGGACACAGTCTTATAAATACTGCTTGAAAAGCTGTTGAACCTGAAGCCAACTCTGGGCTGCCGCTTCAGGCTGA
TAGCTGGCCCGCTGATCACA

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 674; Mature: 673

Protein sequence:

>674_residues
MPKQIIIAEQHRIAAVFSEDQIQELIVATGSHQVGDIYLGIVENVLPGIDAAFVNIGDAERNGFIHVSDLGPVRLKRNSG
AITELLAPQQKVLVQIMKEPTGTKGPRLTGNISLPGRYLVLMPSGRGVNLSRRIRAEDERSRLRALGILIKPAGMGLLVR
TEAEGVAEEAIIEDLELLQRQWESVQQQAASTRAPALLDRDTDFIQRVLRDVYSSNVNRIVVDSTPGLKRVKQHLVNWNN
TNKLPQGVLVDQHRESTSILEYFRVNAAIREALKPRVDLPSGGYIIIEKTEALTVVDVNSGSFTRSATARETVLWTNCEA
AAEIARQLRLRNIAGVIIIDFIDMDSRRDQLQVLEHFNKALKADKARPQIAQLTELGLVELTRKRQGQNIYELFGTPCPA
CSGLGHLVHLPGEAAPTVGEVEWSEPSKPSPTSTSGVPLPGISANLNDQIHLDPSDDLQELDLTNHPSYQERGNRRRRRR
SRLGDNLSRESLSPRLVRNGSASDSKPVKSTNDTNGSTSSKSGGNERSRGRSSTRQRQDTPPEVISVTMTPEEQDIYAFM
GISPTVLFPGEIKKPKSAIVEILAPGETSSTPNTTATKVITKELVVAKDTKADADDIDATKPEEAQAEPVLVATPEPTEE
KPAEPARTSTRRRRRRSSASSAKQLSLDPNESSE

Sequences:

>Translated_674_residues
MPKQIIIAEQHRIAAVFSEDQIQELIVATGSHQVGDIYLGIVENVLPGIDAAFVNIGDAERNGFIHVSDLGPVRLKRNSG
AITELLAPQQKVLVQIMKEPTGTKGPRLTGNISLPGRYLVLMPSGRGVNLSRRIRAEDERSRLRALGILIKPAGMGLLVR
TEAEGVAEEAIIEDLELLQRQWESVQQQAASTRAPALLDRDTDFIQRVLRDVYSSNVNRIVVDSTPGLKRVKQHLVNWNN
TNKLPQGVLVDQHRESTSILEYFRVNAAIREALKPRVDLPSGGYIIIEKTEALTVVDVNSGSFTRSATARETVLWTNCEA
AAEIARQLRLRNIAGVIIIDFIDMDSRRDQLQVLEHFNKALKADKARPQIAQLTELGLVELTRKRQGQNIYELFGTPCPA
CSGLGHLVHLPGEAAPTVGEVEWSEPSKPSPTSTSGVPLPGISANLNDQIHLDPSDDLQELDLTNHPSYQERGNRRRRRR
SRLGDNLSRESLSPRLVRNGSASDSKPVKSTNDTNGSTSSKSGGNERSRGRSSTRQRQDTPPEVISVTMTPEEQDIYAFM
GISPTVLFPGEIKKPKSAIVEILAPGETSSTPNTTATKVITKELVVAKDTKADADDIDATKPEEAQAEPVLVATPEPTEE
KPAEPARTSTRRRRRRSSASSAKQLSLDPNESSE
>Mature_673_residues
PKQIIIAEQHRIAAVFSEDQIQELIVATGSHQVGDIYLGIVENVLPGIDAAFVNIGDAERNGFIHVSDLGPVRLKRNSGA
ITELLAPQQKVLVQIMKEPTGTKGPRLTGNISLPGRYLVLMPSGRGVNLSRRIRAEDERSRLRALGILIKPAGMGLLVRT
EAEGVAEEAIIEDLELLQRQWESVQQQAASTRAPALLDRDTDFIQRVLRDVYSSNVNRIVVDSTPGLKRVKQHLVNWNNT
NKLPQGVLVDQHRESTSILEYFRVNAAIREALKPRVDLPSGGYIIIEKTEALTVVDVNSGSFTRSATARETVLWTNCEAA
AEIARQLRLRNIAGVIIIDFIDMDSRRDQLQVLEHFNKALKADKARPQIAQLTELGLVELTRKRQGQNIYELFGTPCPAC
SGLGHLVHLPGEAAPTVGEVEWSEPSKPSPTSTSGVPLPGISANLNDQIHLDPSDDLQELDLTNHPSYQERGNRRRRRRS
RLGDNLSRESLSPRLVRNGSASDSKPVKSTNDTNGSTSSKSGGNERSRGRSSTRQRQDTPPEVISVTMTPEEQDIYAFMG
ISPTVLFPGEIKKPKSAIVEILAPGETSSTPNTTATKVITKELVVAKDTKADADDIDATKPEEAQAEPVLVATPEPTEEK
PAEPARTSTRRRRRRSSASSAKQLSLDPNESSE

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=410, Percent_Identity=37.0731707317073, Blast_Score=251, Evalue=9e-68,
Organism=Escherichia coli, GI87082235, Length=382, Percent_Identity=38.7434554973822, Blast_Score=233, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 73962; Mature: 73831

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS50126 S1 ; PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQIIIAEQHRIAAVFSEDQIQELIVATGSHQVGDIYLGIVENVLPGIDAAFVNIGDAE
CCCCEEEECCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
RNGFIHVSDLGPVRLKRNSGAITELLAPQQKVLVQIMKEPTGTKGPRLTGNISLPGRYLV
CCCEEEEECCCCEEEECCCCCHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LMPSGRGVNLSRRIRAEDERSRLRALGILIKPAGMGLLVRTEAEGVAEEAIIEDLELLQR
EEECCCCCCHHHHHCCHHHHHHHHHHHEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHH
QWESVQQQAASTRAPALLDRDTDFIQRVLRDVYSSNVNRIVVDSTPGLKRVKQHLVNWNN
HHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCC
TNKLPQGVLVDQHRESTSILEYFRVNAAIREALKPRVDLPSGGYIIIEKTEALTVVDVNS
CCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEEECCC
GSFTRSATARETVLWTNCEAAAEIARQLRLRNIAGVIIIDFIDMDSRRDQLQVLEHFNKA
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
LKADKARPQIAQLTELGLVELTRKRQGQNIYELFGTPCPACSGLGHLVHLPGEAAPTVGE
HHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCC
VEWSEPSKPSPTSTSGVPLPGISANLNDQIHLDPSDDLQELDLTNHPSYQERGNRRRRRR
EECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHH
SRLGDNLSRESLSPRLVRNGSASDSKPVKSTNDTNGSTSSKSGGNERSRGRSSTRQRQDT
HHHCCCCCHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHCCCC
PPEVISVTMTPEEQDIYAFMGISPTVLFPGEIKKPKSAIVEILAPGETSSTPNTTATKVI
CCCEEEEEECCCCCCEEEEECCCCEEECCCCCCCCHHHHEEEECCCCCCCCCCCHHHHHH
TKELVVAKDTKADADDIDATKPEEAQAEPVLVATPEPTEEKPAEPARTSTRRRRRRSSAS
HHHHHEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
SAKQLSLDPNESSE
CCCEEECCCCCCCC
>Mature Secondary Structure 
PKQIIIAEQHRIAAVFSEDQIQELIVATGSHQVGDIYLGIVENVLPGIDAAFVNIGDAE
CCCEEEECCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
RNGFIHVSDLGPVRLKRNSGAITELLAPQQKVLVQIMKEPTGTKGPRLTGNISLPGRYLV
CCCEEEEECCCCEEEECCCCCHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LMPSGRGVNLSRRIRAEDERSRLRALGILIKPAGMGLLVRTEAEGVAEEAIIEDLELLQR
EEECCCCCCHHHHHCCHHHHHHHHHHHEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHH
QWESVQQQAASTRAPALLDRDTDFIQRVLRDVYSSNVNRIVVDSTPGLKRVKQHLVNWNN
HHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCC
TNKLPQGVLVDQHRESTSILEYFRVNAAIREALKPRVDLPSGGYIIIEKTEALTVVDVNS
CCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEEECCC
GSFTRSATARETVLWTNCEAAAEIARQLRLRNIAGVIIIDFIDMDSRRDQLQVLEHFNKA
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
LKADKARPQIAQLTELGLVELTRKRQGQNIYELFGTPCPACSGLGHLVHLPGEAAPTVGE
HHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCC
VEWSEPSKPSPTSTSGVPLPGISANLNDQIHLDPSDDLQELDLTNHPSYQERGNRRRRRR
EECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHH
SRLGDNLSRESLSPRLVRNGSASDSKPVKSTNDTNGSTSSKSGGNERSRGRSSTRQRQDT
HHHCCCCCHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHCCCC
PPEVISVTMTPEEQDIYAFMGISPTVLFPGEIKKPKSAIVEILAPGETSSTPNTTATKVI
CCCEEEEEECCCCCCEEEEECCCCEEECCCCCCCCHHHHEEEECCCCCCCCCCCHHHHHH
TKELVVAKDTKADADDIDATKPEEAQAEPVLVATPEPTEEKPAEPARTSTRRRRRRSSAS
HHHHHEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
SAKQLSLDPNESSE
CCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]