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The map label for this gene is cobS [H]

Identifier: 158334867

GI number: 158334867

Start: 1686106

End: 1686858

Strand: Direct

Name: cobS [H]

Synonym: AM1_1704

Alternate gene names: 158334867

Gene position: 1686106-1686858 (Clockwise)

Preceding gene: 158334865

Following gene: 158334868

Centisome position: 25.93

GC content: 50.86

Gene sequence:

>753_bases
ATGAAACATATTTTGCAGCAAATCGGAGGTGCGATCGCATTTTACAGCTGTATTCCCCTCCCCTCTAGCTGGCCCTTAGA
ATTTGAGCGCATTGCCCGCTGGGCTCCTTGGATTGGTTTTCTCTTAGGAATGGGAATTGGCACTCTCGACTATGGTCTTG
CCCTAGGGCATATGCCTGATCTAACTCGTAGTGCTTTAGTCGTCGCCCTATGGATCGGTCTGACCGGGGGGCTCCATCTG
GATGGCGTTATGGATACCGCCGATGGCCTGGCCGTGATGGAGCCCGAACGGCGTCTTACGGTCATGTCTGATAGTCGAAC
CGGAGCCTTTGGGGTCATGGCTGGCACCCTGGTGCTTCTTCTCAAGGTCTGCGCATTAAGTAACCTCTCGATTGCTCACC
TGCCTGCCCTAGTAACTGCCCTAGTCTGGGGACGAATCGCTCAAGTGATGGCCATTGCCATCTTTCCTTATCTCAAACCT
GAGGGAAATAATGCCTTTCACGCCAACGCTTTTCAGGGGCTAGGGGATCTATGGCCTAGTGTCATTGGTGTATTTGCGAT
CGCAGTTTGCCAATATTACGGGTTTCCCTTAGCATGGCCATCCGTTTTGGGGGGTATCCTAATGGCAAGCAGCCTATCCC
TTGGCATCAGCTACTGGTTTTATTACCAATTTAAAGGAATGACTGGTGATGTTTACGGTGCCATCGTAGAATGGACAGAA
GCATTAATCCTATGCTGCTTGACACTGGTTTAA

Upstream 100 bases:

>100_bases
ATGACTGACCTAGCTATCCTTTACCACTATCAGCTGCTGACTAAATGCAAGGGGATAGCATCATGATTCATCGCTCTTTC
TTTCTTTTTCCTTGGCACAA

Downstream 100 bases:

>100_bases
TCAGTCCCCCTTGTCAAAATCCCATAGGCCAGCGTGAGCATAACCGAGACTCCACGCTATAATGCTTCTCAACAGGAGCT
ATCTCGACTTCGGTACGGCT

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKHILQQIGGAIAFYSCIPLPSSWPLEFERIARWAPWIGFLLGMGIGTLDYGLALGHMPDLTRSALVVALWIGLTGGLHL
DGVMDTADGLAVMEPERRLTVMSDSRTGAFGVMAGTLVLLLKVCALSNLSIAHLPALVTALVWGRIAQVMAIAIFPYLKP
EGNNAFHANAFQGLGDLWPSVIGVFAIAVCQYYGFPLAWPSVLGGILMASSLSLGISYWFYYQFKGMTGDVYGAIVEWTE
ALILCCLTLV

Sequences:

>Translated_250_residues
MKHILQQIGGAIAFYSCIPLPSSWPLEFERIARWAPWIGFLLGMGIGTLDYGLALGHMPDLTRSALVVALWIGLTGGLHL
DGVMDTADGLAVMEPERRLTVMSDSRTGAFGVMAGTLVLLLKVCALSNLSIAHLPALVTALVWGRIAQVMAIAIFPYLKP
EGNNAFHANAFQGLGDLWPSVIGVFAIAVCQYYGFPLAWPSVLGGILMASSLSLGISYWFYYQFKGMTGDVYGAIVEWTE
ALILCCLTLV
>Mature_250_residues
MKHILQQIGGAIAFYSCIPLPSSWPLEFERIARWAPWIGFLLGMGIGTLDYGLALGHMPDLTRSALVVALWIGLTGGLHL
DGVMDTADGLAVMEPERRLTVMSDSRTGAFGVMAGTLVLLLKVCALSNLSIAHLPALVTALVWGRIAQVMAIAIFPYLKP
EGNNAFHANAFQGLGDLWPSVIGVFAIAVCQYYGFPLAWPSVLGGILMASSLSLGISYWFYYQFKGMTGDVYGAIVEWTE
ALILCCLTLV

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:
GO:0005886: Cobalamin synthase
GO:0008818: Cobalamin synthase
GO:0009236: Cobalamin synthase
GO:0015420: Cobalamin synthase
GO:0016020: Cobalamin synthase
GO:0016021: Cobalamin synthase
GO:0016740: Cobalamin synthase

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=29.5546558704453, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26972; Mature: 26972

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHILQQIGGAIAFYSCIPLPSSWPLEFERIARWAPWIGFLLGMGIGTLDYGLALGHMPD
CHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
LTRSALVVALWIGLTGGLHLDGVMDTADGLAVMEPERRLTVMSDSRTGAFGVMAGTLVLL
HHHHHHHHHHHHHHCCCCEECCHHHCCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHH
LKVCALSNLSIAHLPALVTALVWGRIAQVMAIAIFPYLKPEGNNAFHANAFQGLGDLWPS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHH
VIGVFAIAVCQYYGFPLAWPSVLGGILMASSLSLGISYWFYYQFKGMTGDVYGAIVEWTE
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHH
ALILCCLTLV
HHHHHHHHHC
>Mature Secondary Structure
MKHILQQIGGAIAFYSCIPLPSSWPLEFERIARWAPWIGFLLGMGIGTLDYGLALGHMPD
CHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
LTRSALVVALWIGLTGGLHLDGVMDTADGLAVMEPERRLTVMSDSRTGAFGVMAGTLVLL
HHHHHHHHHHHHHHCCCCEECCHHHCCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHH
LKVCALSNLSIAHLPALVTALVWGRIAQVMAIAIFPYLKPEGNNAFHANAFQGLGDLWPS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHH
VIGVFAIAVCQYYGFPLAWPSVLGGILMASSLSLGISYWFYYQFKGMTGDVYGAIVEWTE
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHH
ALILCCLTLV
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA