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The map label for this gene is pyrD

Identifier: 158334803

GI number: 158334803

Start: 1606849

End: 1607973

Strand: Reverse

Name: pyrD

Synonym: AM1_1640

Alternate gene names: 158334803

Gene position: 1607973-1606849 (Counterclockwise)

Preceding gene: 158334804

Following gene: 158334800

Centisome position: 24.72

GC content: 51.47

Gene sequence:

>1125_bases
GTGGATTTATATCAACAAGGGTTACAACCTCTTTTATTTTCGGCTTTGAAAGCTGACCCAGAAACGGTCCATCGTCAATT
GATGCGGACTTGTGCCTGGCTGGATCGGAATGTGGATCAGGGGTTGGCTCAAGGCTTGCAACGGCGGTTGGCCTCTTCTC
TACAGGTGGAAGACCCTCGGCTTTCGCAATCTTTGTGGGGCCTCTCCTTTGCCAACCCCATAGGATTGGCGGCGGGGTTT
GATAAGGATGGTGTGGCAACCAATATTTGGCCTCGTTTTGGGTTTGGATTTGCAGAAGTAGGAACAGTGACCTTCCACGC
TCAACCCGGTAACCCTCAACCTCGCTTGTTTCGTTTACCTGAGGATCAAGCAGCGCTGAATCGGATGGGATTCAATAACC
AAGGGGCTGCTGCGATGGCGAAGGTGATTGCGGCTTCACTGGACCGCCAAGCTCGTTCCTATCCTCTAGGGATTAATTTA
GGCAAGTCAAAAGTTACGCCCCTCGAACAGGCGGTCGCAGATTATGTGGGGAGTTTTCAGCTACTGAAAACCTATGGGGA
CTATTTTGTGGTGAATGTGAGTTCACCGAATACGCCAGGGTTGCGATCGCTTCAAGCGGTTGAACAATTGGCCCCCATTT
TGGCGGGTCTGCAAGCCGAGAATACGGAAGGCAAACCTCTGCTAGTGAAGATTGCCCCAGACTTAGAGTGGAAGGATATT
GCCGCGATTGTAGACTTAGCTCAAGTTCATCAGCTGGCGGGCATTATTGCTACGAACACAACGATTCGGCGGGATCTGAA
AACGGAGCGGATTGCCGCTACGGGGAATGTACCTAGTGAAGAAGCAGGTGGGATAAGTGGTGCCCCGGTGCGATCGCGTT
CCACAGACGTGATTCGATTTATTCACAAACAGACCCAAGGCCAACTGCCAATTATCGGCGTAGGCGGCATCTTTACCGCT
GAGGATGCCTGGGAAAAACTCTGTGCGGGGGCCAGCTTATTACAAGTCTATACAGGCTGGGTCTATGAAGGGCCGTGGAT
GGTGCGCCGAATTTTGGAAGGATTGCTGGTAAAAATGCAGGAGGAAGGCATTCAGCAACTCTCTGAAATCGTGGGTCAGA
AATAG

Upstream 100 bases:

>100_bases
TCGCCTGGGCTGGCCTGTGCAACAATCGTCAGACACTTCTGCGCCCCCTGCTGAACCTTAGCGGGCTATACTCTCAGTGC
GATGCTGGTCTGAGAAAACT

Downstream 100 bases:

>100_bases
GTTGGGTCGTCTCAGTAGATATCAAGATTTAGGTGTTCTTTTTCACCAGACCAACGTCAACACTTCCCTGCCCCCCATAA
AGATACAGCGGCATCAATCT

Product: dihydroorotate oxidase

Products: NA

Alternate protein names: DHOdehase; DHOD; DHODase; Dihydroorotate oxidase

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MDLYQQGLQPLLFSALKADPETVHRQLMRTCAWLDRNVDQGLAQGLQRRLASSLQVEDPRLSQSLWGLSFANPIGLAAGF
DKDGVATNIWPRFGFGFAEVGTVTFHAQPGNPQPRLFRLPEDQAALNRMGFNNQGAAAMAKVIAASLDRQARSYPLGINL
GKSKVTPLEQAVADYVGSFQLLKTYGDYFVVNVSSPNTPGLRSLQAVEQLAPILAGLQAENTEGKPLLVKIAPDLEWKDI
AAIVDLAQVHQLAGIIATNTTIRRDLKTERIAATGNVPSEEAGGISGAPVRSRSTDVIRFIHKQTQGQLPIIGVGGIFTA
EDAWEKLCAGASLLQVYTGWVYEGPWMVRRILEGLLVKMQEEGIQQLSEIVGQK

Sequences:

>Translated_374_residues
MDLYQQGLQPLLFSALKADPETVHRQLMRTCAWLDRNVDQGLAQGLQRRLASSLQVEDPRLSQSLWGLSFANPIGLAAGF
DKDGVATNIWPRFGFGFAEVGTVTFHAQPGNPQPRLFRLPEDQAALNRMGFNNQGAAAMAKVIAASLDRQARSYPLGINL
GKSKVTPLEQAVADYVGSFQLLKTYGDYFVVNVSSPNTPGLRSLQAVEQLAPILAGLQAENTEGKPLLVKIAPDLEWKDI
AAIVDLAQVHQLAGIIATNTTIRRDLKTERIAATGNVPSEEAGGISGAPVRSRSTDVIRFIHKQTQGQLPIIGVGGIFTA
EDAWEKLCAGASLLQVYTGWVYEGPWMVRRILEGLLVKMQEEGIQQLSEIVGQK
>Mature_374_residues
MDLYQQGLQPLLFSALKADPETVHRQLMRTCAWLDRNVDQGLAQGLQRRLASSLQVEDPRLSQSLWGLSFANPIGLAAGF
DKDGVATNIWPRFGFGFAEVGTVTFHAQPGNPQPRLFRLPEDQAALNRMGFNNQGAAAMAKVIAASLDRQARSYPLGINL
GKSKVTPLEQAVADYVGSFQLLKTYGDYFVVNVSSPNTPGLRSLQAVEQLAPILAGLQAENTEGKPLLVKIAPDLEWKDI
AAIVDLAQVHQLAGIIATNTTIRRDLKTERIAATGNVPSEEAGGISGAPVRSRSTDVIRFIHKQTQGQLPIIGVGGIFTA
EDAWEKLCAGASLLQVYTGWVYEGPWMVRRILEGLLVKMQEEGIQQLSEIVGQK

Specific function: Pyrimidine biosynthesis; fourth step. [C]

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:
GO:0003824: Dihydroorotate oxidase, putative
GO:0004152: Dihydroorotate oxidase, putative
GO:0006221: Dihydroorotate oxidase, putative
GO:0006222: Dihydroorotate oxidase, putative
GO:0008152: Dihydroorotate oxidase, putative
GO:0016491: Dihydroorotate oxidase, putative
GO:0055114: Dihydroorotate oxidase, putative
GO:0004158: Dihydroorotate dehydrogenase (quinone)
GO:0005886: Dihydroorotate dehydrogenase (quinone)
GO:0006207: Dihydroorotate dehydrogenase (quinone)
GO:0016020: Dihydroorotate dehydrogenase (quinone)

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI45006951, Length=379, Percent_Identity=39.8416886543536, Blast_Score=241, Evalue=9e-64,
Organism=Escherichia coli, GI1787177, Length=296, Percent_Identity=42.5675675675676, Blast_Score=238, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI17509475, Length=384, Percent_Identity=41.1458333333333, Blast_Score=253, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6322633, Length=277, Percent_Identity=25.2707581227437, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI281361352, Length=324, Percent_Identity=40.1234567901235, Blast_Score=238, Evalue=7e-63,
Organism=Drosophila melanogaster, GI17137316, Length=324, Percent_Identity=40.1234567901235, Blast_Score=238, Evalue=7e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRD_ACAM1 (B0CA74)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001515975.1
- ProteinModelPortal:   B0CA74
- SMR:   B0CA74
- GeneID:   5680456
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1640
- HOGENOM:   HBG351027
- OMA:   AALNRMG
- ProtClustDB:   CLSK2475789
- BioCyc:   AMAR329726:AM1_1640-MONOMER
- HAMAP:   MF_00225
- InterPro:   IPR013785
- InterPro:   IPR012135
- InterPro:   IPR005719
- InterPro:   IPR001295
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000164
- TIGRFAMs:   TIGR01036

Pfam domain/function: PF01180 DHO_dh

EC number: =1.3.5.2

Molecular weight: Translated: 40679; Mature: 40679

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00911 DHODEHASE_1; PS00912 DHODEHASE_2

Important sites: ACT_SITE 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLYQQGLQPLLFSALKADPETVHRQLMRTCAWLDRNVDQGLAQGLQRRLASSLQVEDPR
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCH
LSQSLWGLSFANPIGLAAGFDKDGVATNIWPRFGFGFAEVGTVTFHAQPGNPQPRLFRLP
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCEEEEEECCCCCCCCEEECC
EDQAALNRMGFNNQGAAAMAKVIAASLDRQARSYPLGINLGKSKVTPLEQAVADYVGSFQ
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHH
LLKTYGDYFVVNVSSPNTPGLRSLQAVEQLAPILAGLQAENTEGKPLLVKIAPDLEWKDI
HHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHH
AAIVDLAQVHQLAGIIATNTTIRRDLKTERIAATGNVPSEEAGGISGAPVRSRSTDVIRF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCCHHCCCCCCCCCCCCCHHHHHH
IHKQTQGQLPIIGVGGIFTAEDAWEKLCAGASLLQVYTGWVYEGPWMVRRILEGLLVKMQ
HHHHHCCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
EEGIQQLSEIVGQK
HHHHHHHHHHHCCC
>Mature Secondary Structure
MDLYQQGLQPLLFSALKADPETVHRQLMRTCAWLDRNVDQGLAQGLQRRLASSLQVEDPR
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCH
LSQSLWGLSFANPIGLAAGFDKDGVATNIWPRFGFGFAEVGTVTFHAQPGNPQPRLFRLP
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCEEEEEECCCCCCCCEEECC
EDQAALNRMGFNNQGAAAMAKVIAASLDRQARSYPLGINLGKSKVTPLEQAVADYVGSFQ
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHH
LLKTYGDYFVVNVSSPNTPGLRSLQAVEQLAPILAGLQAENTEGKPLLVKIAPDLEWKDI
HHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHH
AAIVDLAQVHQLAGIIATNTTIRRDLKTERIAATGNVPSEEAGGISGAPVRSRSTDVIRF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCCHHCCCCCCCCCCCCCHHHHHH
IHKQTQGQLPIIGVGGIFTAEDAWEKLCAGASLLQVYTGWVYEGPWMVRRILEGLLVKMQ
HHHHHCCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
EEGIQQLSEIVGQK
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA