Click here to switch to the map view.

The map label for this gene is gcvH

Identifier: 158334698

GI number: 158334698

Start: 1513993

End: 1514379

Strand: Reverse

Name: gcvH

Synonym: AM1_1531

Alternate gene names: 158334698

Gene position: 1514379-1513993 (Counterclockwise)

Preceding gene: 158334699

Following gene: 158334697

Centisome position: 23.28

GC content: 46.25

Gene sequence:

>387_bases
ATGAGTTTTGAATATCCCGACAATTTGAAATATATGGACTCCCATGAATATGTTCGTTTGGAGGGAGATGTTGCCACGGT
TGGTATCACCGCATTTGCCATCGATCAGTTAGGGGATATTGTCTTTATTGAATTGCCAGAAGTGGGTGATGAATTATCCC
AAGGGGAGACGATGGGAAATATCGAATCCGTCAAAGCCGTAGAAGATATTTATGCTCCGATTAATGGAACAGTATTGGAG
TGCAATACCGCCATTGCCGATGCCCCTGAACAACTCGCAGAAGATCCCTACAATAACGGCTGGCTACTCAAGATTAAGGT
GAGTGGGCCTGCCGCTTTAGACAAGGCCATGTCGGCGTCAGACTACCAGGCTAAGGTCGAAGGCTAA

Upstream 100 bases:

>100_bases
TTTTATCGACGACCTAAAGCGTGAGATTTAAGGCAAAATATGGTTTGGATGATTCTCCTGAAATCCTACTCAACTTCCTT
ATTGACTGAGGCACCCCATT

Downstream 100 bases:

>100_bases
CCCCGAATTATCTCATAAATCCCTGAGAAAACTTTACAATTTTTTACATCCAAAAAAGCTTATAAGGTTCAACAAATCGT
TTGTCAAGCTATTTTCGCTT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MSFEYPDNLKYMDSHEYVRLEGDVATVGITAFAIDQLGDIVFIELPEVGDELSQGETMGNIESVKAVEDIYAPINGTVLE
CNTAIADAPEQLAEDPYNNGWLLKIKVSGPAALDKAMSASDYQAKVEG

Sequences:

>Translated_128_residues
MSFEYPDNLKYMDSHEYVRLEGDVATVGITAFAIDQLGDIVFIELPEVGDELSQGETMGNIESVKAVEDIYAPINGTVLE
CNTAIADAPEQLAEDPYNNGWLLKIKVSGPAALDKAMSASDYQAKVEG
>Mature_127_residues
SFEYPDNLKYMDSHEYVRLEGDVATVGITAFAIDQLGDIVFIELPEVGDELSQGETMGNIESVKAVEDIYAPINGTVLEC
NTAIADAPEQLAEDPYNNGWLLKIKVSGPAALDKAMSASDYQAKVEG

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:
GO:0005960: Glycine cleavage system H protein
GO:0006546: Glycine cleavage system H protein
GO:0019464: Glycine cleavage system H protein

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=118, Percent_Identity=40.6779661016949, Blast_Score=105, Evalue=9e-24,
Organism=Homo sapiens, GI89057342, Length=118, Percent_Identity=39.8305084745763, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1789271, Length=123, Percent_Identity=45.5284552845528, Blast_Score=111, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17507493, Length=112, Percent_Identity=47.3214285714286, Blast_Score=113, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17551294, Length=112, Percent_Identity=43.75, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=107, Percent_Identity=47.6635514018692, Blast_Score=108, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17865652, Length=116, Percent_Identity=39.6551724137931, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_ACAM1 (B0C921)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001515870.1
- ProteinModelPortal:   B0C921
- SMR:   B0C921
- GeneID:   5680351
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1531
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- BioCyc:   AMAR329726:AM1_1531-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13862; Mature: 13730

Theoretical pI: Translated: 3.74; Mature: 3.74

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFEYPDNLKYMDSHEYVRLEGDVATVGITAFAIDQLGDIVFIELPEVGDELSQGETMGN
CCCCCCCCCEEECCCCEEEEECCEEEEHHHHHHHHCCCCEEEEECCCCCCHHHCCCCCCC
IESVKAVEDIYAPINGTVLECNTAIADAPEQLAEDPYNNGWLLKIKVSGPAALDKAMSAS
HHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHCCCCCCCEEEEEEECCCHHHHHHCCCC
DYQAKVEG
CCEEEECC
>Mature Secondary Structure 
SFEYPDNLKYMDSHEYVRLEGDVATVGITAFAIDQLGDIVFIELPEVGDELSQGETMGN
CCCCCCCCEEECCCCEEEEECCEEEEHHHHHHHHCCCCEEEEECCCCCCHHHCCCCCCC
IESVKAVEDIYAPINGTVLECNTAIADAPEQLAEDPYNNGWLLKIKVSGPAALDKAMSAS
HHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHCCCCCCCEEEEEEECCCHHHHHHCCCC
DYQAKVEG
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA