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The map label for this gene is gshA

Identifier: 158334677

GI number: 158334677

Start: 1498233

End: 1499378

Strand: Reverse

Name: gshA

Synonym: AM1_1510

Alternate gene names: NA

Gene position: 1499378-1498233 (Counterclockwise)

Preceding gene: 158334680

Following gene: 158334676

Centisome position: 23.05

GC content: 49.65

Gene sequence:

>1146_bases
ATGGTGCTTTCTAAAGGGTTCGAGGTAGAACTGTATACTGGGAAACCCTCAGGTGAGATTGTTGGCCTGTCCGACCGAAT
TGTTGCCGATTTAGATGGGTTTGTTCGAGAACCTGACAGTCGTAATGTCGAATATACGACTGCCCCAGCCCATCATTATG
ATCCTCTGCTTTGTGATCTGCTCAAACCTCGTCTCCAGCTTCGCGAATATGTGCGAACCCTGGGAGACTACACCCTGATT
CCAGGCAGTACCCTGGCCCTGTCAGATCATCAGCATTTTTATCGGTCTGATCCCAAGAATCCATACCATTCCTATATTGA
GCAGACCTACGGTACGAGTGTTGTGACGGCCAGTATTCATATCAATATTGGTATTCCTGACATTGAGCAGCTCTTCCAGG
CATGTCGCCTAGTTCGGATGGAAGCCCCACTATTTTTGGCCTTAAGTGCCTCTTCTCCCTTTTTAGGGGAGCAGGTGACG
GGCCATCATTCGTCTCGTTGGGCAGTCTTCCCCAAAACCCCAGCCCAGGTGCCCCTGTTTACCAGTCATCAGCATTTTAT
TGATTGGACCGAGTCACAGCTCTTACAGGGCACGATGCAAAATGTGCGCCACTTGTGGAGTTCGGTTCGCCCCAACGGCA
ATCGTCGTCCCTATGATTTGAATCGCTTGGAGTTGAGAATTTGCGATCTCGTCACTCATCCCATCCATTTATTAGCGATT
ACGGCGTTGCTAGAAGCCCGAATCCTCCAGCTGCTCGATGATCCCCAGCTTGATCCTTTGGTGGGTAGCAACTTCAAGAC
TGATTCTGAACACCTGGTTGCTCTAGCAGATGCCAATGAGATCGCTGCTGCTACCCATAGTTTGGATGCGGAATTGACCC
ATTGGCAAGATGGCCGCAAAATTCTGGCCCGAGACTGGATTGAAGAGCTGTATACCGAAGTATGGCCGACGGCGAAACAA
TCAGGATTTAGCTGTTTTCTGTCGCCAATTCACCAGATTTTGGACCAAGGGAATGAGGCTCAACAGTGGTTGAGACAACA
TCAACAGGGGCAGTCGATTCGGTCTGTGATGCAGGATGCGATCGCAAACATGGCCCAAACAGAGATTGATCTGCGCAACA
AATTATGTGAGCCTGTAGCGGTATAA

Upstream 100 bases:

>100_bases
CACTCAACCTTGGTTTGCCCTCATAAAATTTACTAACTCTTTCTAAGGGTCTATTCAGATCTGCTGTCTATAATCTGTTT
GACAAGAGCGAGGGATATGC

Downstream 100 bases:

>100_bases
AAAGTCCACCAGCCAACGTCTAAGCCATGCTCCGGGTTGTCCTTGTTTATCCTGAGATCCCTCCCAACACAGGTAATATT
GCTCGGACCTGTGCGGCAAC

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MVLSKGFEVELYTGKPSGEIVGLSDRIVADLDGFVREPDSRNVEYTTAPAHHYDPLLCDLLKPRLQLREYVRTLGDYTLI
PGSTLALSDHQHFYRSDPKNPYHSYIEQTYGTSVVTASIHINIGIPDIEQLFQACRLVRMEAPLFLALSASSPFLGEQVT
GHHSSRWAVFPKTPAQVPLFTSHQHFIDWTESQLLQGTMQNVRHLWSSVRPNGNRRPYDLNRLELRICDLVTHPIHLLAI
TALLEARILQLLDDPQLDPLVGSNFKTDSEHLVALADANEIAAATHSLDAELTHWQDGRKILARDWIEELYTEVWPTAKQ
SGFSCFLSPIHQILDQGNEAQQWLRQHQQGQSIRSVMQDAIANMAQTEIDLRNKLCEPVAV

Sequences:

>Translated_381_residues
MVLSKGFEVELYTGKPSGEIVGLSDRIVADLDGFVREPDSRNVEYTTAPAHHYDPLLCDLLKPRLQLREYVRTLGDYTLI
PGSTLALSDHQHFYRSDPKNPYHSYIEQTYGTSVVTASIHINIGIPDIEQLFQACRLVRMEAPLFLALSASSPFLGEQVT
GHHSSRWAVFPKTPAQVPLFTSHQHFIDWTESQLLQGTMQNVRHLWSSVRPNGNRRPYDLNRLELRICDLVTHPIHLLAI
TALLEARILQLLDDPQLDPLVGSNFKTDSEHLVALADANEIAAATHSLDAELTHWQDGRKILARDWIEELYTEVWPTAKQ
SGFSCFLSPIHQILDQGNEAQQWLRQHQQGQSIRSVMQDAIANMAQTEIDLRNKLCEPVAV
>Mature_381_residues
MVLSKGFEVELYTGKPSGEIVGLSDRIVADLDGFVREPDSRNVEYTTAPAHHYDPLLCDLLKPRLQLREYVRTLGDYTLI
PGSTLALSDHQHFYRSDPKNPYHSYIEQTYGTSVVTASIHINIGIPDIEQLFQACRLVRMEAPLFLALSASSPFLGEQVT
GHHSSRWAVFPKTPAQVPLFTSHQHFIDWTESQLLQGTMQNVRHLWSSVRPNGNRRPYDLNRLELRICDLVTHPIHLLAI
TALLEARILQLLDDPQLDPLVGSNFKTDSEHLVALADANEIAAATHSLDAELTHWQDGRKILARDWIEELYTEVWPTAKQ
SGFSCFLSPIHQILDQGNEAQQWLRQHQQGQSIRSVMQDAIANMAQTEIDLRNKLCEPVAV

Specific function: ATP-dependent carboxylate-amine ligase [H]

COG id: COG2170

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carboxylate-amine ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011793
- InterPro:   IPR006336 [H]

Pfam domain/function: PF04107 GCS2 [H]

EC number: NA

Molecular weight: Translated: 43119; Mature: 43119

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLSKGFEVELYTGKPSGEIVGLSDRIVADLDGFVREPDSRNVEYTTAPAHHYDPLLCDL
CCCCCCCEEEEEECCCCCCEECCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHH
LKPRLQLREYVRTLGDYTLIPGSTLALSDHQHFYRSDPKNPYHSYIEQTYGTSVVTASIH
HHHHHHHHHHHHHHCCEEECCCCEEEECCCHHHHHCCCCCHHHHHHHHHCCCEEEEEEEE
INIGIPDIEQLFQACRLVRMEAPLFLALSASSPFLGEQVTGHHSSRWAVFPKTPAQVPLF
EEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCEE
TSHQHFIDWTESQLLQGTMQNVRHLWSSVRPNGNRRPYDLNRLELRICDLVTHPIHLLAI
ECCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TALLEARILQLLDDPQLDPLVGSNFKTDSEHLVALADANEIAAATHSLDAELTHWQDGRK
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHCCHH
ILARDWIEELYTEVWPTAKQSGFSCFLSPIHQILDQGNEAQQWLRQHQQGQSIRSVMQDA
HHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHH
IANMAQTEIDLRNKLCEPVAV
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVLSKGFEVELYTGKPSGEIVGLSDRIVADLDGFVREPDSRNVEYTTAPAHHYDPLLCDL
CCCCCCCEEEEEECCCCCCEECCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHH
LKPRLQLREYVRTLGDYTLIPGSTLALSDHQHFYRSDPKNPYHSYIEQTYGTSVVTASIH
HHHHHHHHHHHHHHCCEEECCCCEEEECCCHHHHHCCCCCHHHHHHHHHCCCEEEEEEEE
INIGIPDIEQLFQACRLVRMEAPLFLALSASSPFLGEQVTGHHSSRWAVFPKTPAQVPLF
EEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCEE
TSHQHFIDWTESQLLQGTMQNVRHLWSSVRPNGNRRPYDLNRLELRICDLVTHPIHLLAI
ECCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TALLEARILQLLDDPQLDPLVGSNFKTDSEHLVALADANEIAAATHSLDAELTHWQDGRK
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCHHHHHHCCHH
ILARDWIEELYTEVWPTAKQSGFSCFLSPIHQILDQGNEAQQWLRQHQQGQSIRSVMQDA
HHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHH
IANMAQTEIDLRNKLCEPVAV
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA