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The map label for this gene is yibK [C]

Identifier: 158334676

GI number: 158334676

Start: 1497745

End: 1498206

Strand: Reverse

Name: yibK [C]

Synonym: AM1_1509

Alternate gene names: 158334676

Gene position: 1498206-1497745 (Counterclockwise)

Preceding gene: 158334677

Following gene: 158334675

Centisome position: 23.04

GC content: 48.48

Gene sequence:

>462_bases
ATGCTCCGGGTTGTCCTTGTTTATCCTGAGATCCCTCCCAACACAGGTAATATTGCTCGGACCTGTGCGGCAACGCAAAC
CGAGCTGCATTTGGTTGAGCCTTTAGGGTTTGAAATATCCAATCGGTATTTAAAGCGGGCAGGACTAGACTACTGGCCCT
TTGTAAAACTCCAAACCCATGCTTCTTGGGCTGCCTTTGAGCAGGTGCGTCAGCAACAAGGTGGACGTTCCGTTAGCTTC
AGCAAATCTGGAACCCATAGCTACTTGCAATTCCAATTCTGCGAGGATGATTGGCTGATCTTTGGGAGTGAATCCAGTGG
TTTACCCCCAGATATTCTGCAAGATAGCTCTGCGGTGCTTCGTATCCCGATGGCCCATGAGCAGGTTAGAAGTTTGAACC
TGTCTGTCAGTGCTGCTGTTGGACTATTTGAAGCTCGTAGACAGCTTGCATTAATGACTTAA

Upstream 100 bases:

>100_bases
AGGATGCGATCGCAAACATGGCCCAAACAGAGATTGATCTGCGCAACAAATTATGTGAGCCTGTAGCGGTATAAAAAGTC
CACCAGCCAACGTCTAAGCC

Downstream 100 bases:

>100_bases
CCCTCAGGCTTGAAATGAGGTGAATCTGCACAGTACCGCCCCTAATTTTGGCAGATTCAAATAGATAGATTAGGATAGAT
GGAATATTTTTCTCCATAGA

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MLRVVLVYPEIPPNTGNIARTCAATQTELHLVEPLGFEISNRYLKRAGLDYWPFVKLQTHASWAAFEQVRQQQGGRSVSF
SKSGTHSYLQFQFCEDDWLIFGSESSGLPPDILQDSSAVLRIPMAHEQVRSLNLSVSAAVGLFEARRQLALMT

Sequences:

>Translated_153_residues
MLRVVLVYPEIPPNTGNIARTCAATQTELHLVEPLGFEISNRYLKRAGLDYWPFVKLQTHASWAAFEQVRQQQGGRSVSF
SKSGTHSYLQFQFCEDDWLIFGSESSGLPPDILQDSSAVLRIPMAHEQVRSLNLSVSAAVGLFEARRQLALMT
>Mature_153_residues
MLRVVLVYPEIPPNTGNIARTCAATQTELHLVEPLGFEISNRYLKRAGLDYWPFVKLQTHASWAAFEQVRQQQGGRSVSF
SKSGTHSYLQFQFCEDDWLIFGSESSGLPPDILQDSSAVLRIPMAHEQVRSLNLSVSAAVGLFEARRQLALMT

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=152, Percent_Identity=42.7631578947368, Blast_Score=119, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17140; Mature: 17140

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVVLVYPEIPPNTGNIARTCAATQTELHLVEPLGFEISNRYLKRAGLDYWPFVKLQTH
CEEEEEEECCCCCCCCCEEHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECC
ASWAAFEQVRQQQGGRSVSFSKSGTHSYLQFQFCEDDWLIFGSESSGLPPDILQDSSAVL
CCHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEECCCEEEEECCCCCCCHHHHCCCCCEE
RIPMAHEQVRSLNLSVSAAVGLFEARRQLALMT
EECCCHHHHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLRVVLVYPEIPPNTGNIARTCAATQTELHLVEPLGFEISNRYLKRAGLDYWPFVKLQTH
CEEEEEEECCCCCCCCCEEHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECC
ASWAAFEQVRQQQGGRSVSFSKSGTHSYLQFQFCEDDWLIFGSESSGLPPDILQDSSAVL
CCHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEECCCEEEEECCCCCCCHHHHCCCCCEE
RIPMAHEQVRSLNLSVSAAVGLFEARRQLALMT
EECCCHHHHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]