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The map label for this gene is nifS [H]

Identifier: 158334400

GI number: 158334400

Start: 1200644

End: 1201906

Strand: Reverse

Name: nifS [H]

Synonym: AM1_1221

Alternate gene names: 158334400

Gene position: 1201906-1200644 (Counterclockwise)

Preceding gene: 158334401

Following gene: 158334399

Centisome position: 18.48

GC content: 51.07

Gene sequence:

>1263_bases
ATGATTGCCATCCAAGAAAAGTCTCTAGCCCTTCAGGTTCGTCCTGACTTTCCCATTTTGCATCAGCAGATTCAGGAGAG
TCCCTTGGTCTATTTGGATAGTGCGGCGACGTCTCAGAAACCGACCTCTGTCTTGGCGGCACTGCAGGATTATTACGAGC
AGGATAATGCCAATGTCCACCGAGGCGTCCATACCCTCAGTGGTCGGGCCACGGATGCCTATGAAGGGGCACGGGATAAG
GTGGCTGCCTTTGTCAATGCGGCGTCTCGGCAGGAGATTGTCTATACCCGCAATGCCAGTGAAGGGATTAACCTGGTGGC
TTATAGCTGGGGAATGAATAATCTCAAGGCTGGGGATGAAGTCATCCTGACGGTGATGGAGCATCACAGTAATTTGATTC
CCTGGCAGTGGGTGGCTCAGAAAACTGGAGCGGTGCTCAAGTTTGTGGAGCTGGATGAGAATCAAGCCTTTGATTTAGAG
CAGTTTAAAACCCTGATCAATGACCGCACCAAACTAGTGGGGGTGGTGCATGTCTCCAATACCTTGGGCTGTATTAATCC
TGTGGAAGAGATTGTTGTGATCGCACACCAATACGATGCCAAAGTGCTGATCGACGCCTGCCAAAGCGTCCCCCATATGC
CCATCGATGTCCAGAAGATAGACTGCGATTGGCTGGTGGCCTCTGGCCATAAAATGTGTGCTCCCACCGGCATTGGCTTC
CTCTACGGCAAACTGGATTTGCTCCGCAGTATGCCTCCGTTCTTGGGTGGCGGTGAAATGATTGCAGATGTATTCCTAGA
CCATGCCACCTATGCAGATTTACCCCATAAATTTGAGGCAGGCACCCCTGCCATTGGTGAGGCAGTGGGCCTAGGGGCTG
CGGTTGATTACTTGACCCAAATTGGTATGGACAAGATTCATGCCTATGAAGCGGAGTTAACGGCTTACCTGTTTAAGCGG
CTGCGGGAGATTCCTGAGGTGCAGATTTATGGTCCTCAGCCCCATGCGGATGGCACGGGTCGAGCGGCATTGGCTTCCTT
TACCGCAGGGGATGTGCATCCCCATGATCTCTCCACGATTTTGGATCAAGCAGGTGTAGCCATTCGGGCCGGACATCACT
GTACTCAGCCTTTGCATCGGCATCTGAAGGTGTCGTCTACGGCCAGAGCAAGTCTCTATTTTTACAATACTCGCGAAGAA
ATAGATGCGTTTATCAACTCACTTAAAGAAGCCGTAGAATTTTTCAGCAGTATCTTTGGATAA

Upstream 100 bases:

>100_bases
TGCGGCGGAAATTATTGATCAATTGCCCATTGCTGAACAGCGCCAGACTTTGCTGAACGCTGTGTTGTCCCAAATACGTT
AGATTCGTGATTCTGCACCT

Downstream 100 bases:

>100_bases
GCGCCAGTGAGCTGTAGCTCCTATGGTTGCTTCCGTCCAAATTCAGCAAATCAATCTTCCTCCGGGTCGGTGAGTTTTGC
TGAATAGGATTAGCTGGGTG

Product: SufS subfamily cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MIAIQEKSLALQVRPDFPILHQQIQESPLVYLDSAATSQKPTSVLAALQDYYEQDNANVHRGVHTLSGRATDAYEGARDK
VAAFVNAASRQEIVYTRNASEGINLVAYSWGMNNLKAGDEVILTVMEHHSNLIPWQWVAQKTGAVLKFVELDENQAFDLE
QFKTLINDRTKLVGVVHVSNTLGCINPVEEIVVIAHQYDAKVLIDACQSVPHMPIDVQKIDCDWLVASGHKMCAPTGIGF
LYGKLDLLRSMPPFLGGGEMIADVFLDHATYADLPHKFEAGTPAIGEAVGLGAAVDYLTQIGMDKIHAYEAELTAYLFKR
LREIPEVQIYGPQPHADGTGRAALASFTAGDVHPHDLSTILDQAGVAIRAGHHCTQPLHRHLKVSSTARASLYFYNTREE
IDAFINSLKEAVEFFSSIFG

Sequences:

>Translated_420_residues
MIAIQEKSLALQVRPDFPILHQQIQESPLVYLDSAATSQKPTSVLAALQDYYEQDNANVHRGVHTLSGRATDAYEGARDK
VAAFVNAASRQEIVYTRNASEGINLVAYSWGMNNLKAGDEVILTVMEHHSNLIPWQWVAQKTGAVLKFVELDENQAFDLE
QFKTLINDRTKLVGVVHVSNTLGCINPVEEIVVIAHQYDAKVLIDACQSVPHMPIDVQKIDCDWLVASGHKMCAPTGIGF
LYGKLDLLRSMPPFLGGGEMIADVFLDHATYADLPHKFEAGTPAIGEAVGLGAAVDYLTQIGMDKIHAYEAELTAYLFKR
LREIPEVQIYGPQPHADGTGRAALASFTAGDVHPHDLSTILDQAGVAIRAGHHCTQPLHRHLKVSSTARASLYFYNTREE
IDAFINSLKEAVEFFSSIFG
>Mature_420_residues
MIAIQEKSLALQVRPDFPILHQQIQESPLVYLDSAATSQKPTSVLAALQDYYEQDNANVHRGVHTLSGRATDAYEGARDK
VAAFVNAASRQEIVYTRNASEGINLVAYSWGMNNLKAGDEVILTVMEHHSNLIPWQWVAQKTGAVLKFVELDENQAFDLE
QFKTLINDRTKLVGVVHVSNTLGCINPVEEIVVIAHQYDAKVLIDACQSVPHMPIDVQKIDCDWLVASGHKMCAPTGIGF
LYGKLDLLRSMPPFLGGGEMIADVFLDHATYADLPHKFEAGTPAIGEAVGLGAAVDYLTQIGMDKIHAYEAELTAYLFKR
LREIPEVQIYGPQPHADGTGRAALASFTAGDVHPHDLSTILDQAGVAIRAGHHCTQPLHRHLKVSSTARASLYFYNTREE
IDAFINSLKEAVEFFSSIFG

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine [H]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:
GO:0003824: Cysteine desulfurase, SufS subfamily
GO:0006534: Cysteine desulfurase, SufS subfamily
GO:0008152: Cysteine desulfurase, SufS subfamily
GO:0016740: Cysteine desulfurase, SufS subfamily
GO:0030170: Cysteine desulfurase, SufS subfamily
GO:0031071: Cysteine desulfurase, SufS subfamily

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=392, Percent_Identity=25.765306122449, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1787970, Length=400, Percent_Identity=45.25, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1789175, Length=404, Percent_Identity=38.1188118811881, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI48994898, Length=403, Percent_Identity=26.0545905707196, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25143064, Length=280, Percent_Identity=26.7857142857143, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI193211090, Length=376, Percent_Identity=25.7978723404255, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=26.4339152119701, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20129463, Length=372, Percent_Identity=28.7634408602151, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010970
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 46174; Mature: 46174

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIQEKSLALQVRPDFPILHQQIQESPLVYLDSAATSQKPTSVLAALQDYYEQDNANVH
CEEEECCCEEEEECCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHH
RGVHTLSGRATDAYEGARDKVAAFVNAASRQEIVYTRNASEGINLVAYSWGMNNLKAGDE
HCHHHHCCCCCCHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCCCCCHH
VILTVMEHHSNLIPWQWVAQKTGAVLKFVELDENQAFDLEQFKTLINDRTKLVGVVHVSN
HHHHHHHHCCCCCCHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEEEECC
TLGCINPVEEIVVIAHQYDAKVLIDACQSVPHMPIDVQKIDCDWLVASGHKMCAPTGIGF
CCCCCCCHHHHEEEHHCCCHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCEECCCCCHH
LYGKLDLLRSMPPFLGGGEMIADVFLDHATYADLPHKFEAGTPAIGEAVGLGAAVDYLTQ
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IGMDKIHAYEAELTAYLFKRLREIPEVQIYGPQPHADGTGRAALASFTAGDVHPHDLSTI
HCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHHH
LDQAGVAIRAGHHCTQPLHRHLKVSSTARASLYFYNTREEIDAFINSLKEAVEFFSSIFG
HHHCCCEEECCCHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAIQEKSLALQVRPDFPILHQQIQESPLVYLDSAATSQKPTSVLAALQDYYEQDNANVH
CEEEECCCEEEEECCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHH
RGVHTLSGRATDAYEGARDKVAAFVNAASRQEIVYTRNASEGINLVAYSWGMNNLKAGDE
HCHHHHCCCCCCHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCCCCCHH
VILTVMEHHSNLIPWQWVAQKTGAVLKFVELDENQAFDLEQFKTLINDRTKLVGVVHVSN
HHHHHHHHCCCCCCHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEEEECC
TLGCINPVEEIVVIAHQYDAKVLIDACQSVPHMPIDVQKIDCDWLVASGHKMCAPTGIGF
CCCCCCCHHHHEEEHHCCCHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCEECCCCCHH
LYGKLDLLRSMPPFLGGGEMIADVFLDHATYADLPHKFEAGTPAIGEAVGLGAAVDYLTQ
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IGMDKIHAYEAELTAYLFKRLREIPEVQIYGPQPHADGTGRAALASFTAGDVHPHDLSTI
HCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHHH
LDQAGVAIRAGHHCTQPLHRHLKVSSTARASLYFYNTREEIDAFINSLKEAVEFFSSIFG
HHHCCCEEECCCHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]