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The map label for this gene is iaaA [H]

Identifier: 158334272

GI number: 158334272

Start: 1063428

End: 1064363

Strand: Direct

Name: iaaA [H]

Synonym: AM1_1092

Alternate gene names: 158334272

Gene position: 1063428-1064363 (Clockwise)

Preceding gene: 158334271

Following gene: 158334275

Centisome position: 16.35

GC content: 50.43

Gene sequence:

>936_bases
ATGTCTTCTACCTACTCCTTAATGATTCATGGGGGCGCTGGTGCCCTACAACATATTCAAGCCGAGGGCAGTGAAGAGGA
CTTTGCCCAAAGTATCAATGGCATTTTGGAGCAGGGACGCCAAAAGCTGCAGCAGGGGGAGCTTGCTCTAAATGTGGTAG
AGCATTGTGTCAGCTTGTTAGAAGATGATCCGCTGTACAACGCTGGACGCGGATCCGTCCTCAATGAGTATGGCGAAGTA
GAGATGGATGCGGCCATTATGAATGGTCAAGATTTGGCTGCAGGTGCAATCACAGGCATCAAAAATATCAAGAACCCAAT
TTCTCTTGCCCGCCTAGTACTCAAAAAAAGCGAGCATGTAATGCTGGTGGGCCATGGTGCCATGGAGTTCGCTGAATTAT
GGGGCGTGGAATGCTTACCTGATGAGTATTTTGTGATTGAAGCGCGCGTGAAACAGTTTCAAGAAGCCAAAAAGGCGGGC
AAGATGGTGCTGGATCATGAAGATATTGAGCAGGAACCCCAACGCAAGTTTGGAACCGTAGGCGCGACAGCCTTTGACTT
GGAAGGCAATTTAGCGGCAGCCACCTCAACGGGGGGCATTGTTAATAAGCGATGGGGGCGTGTGGGCGACTCGCCGATTA
TTGGTGCTGGCGTATTTGCGGACAATGAGACCTGTGCAGTTTCGGCAACGGGATATGGAGAGCAGTTTATGCGGACTGTG
CTTGCCAAAACGATTTCAGACCATATTCTCTTCCAATCTCTGACTGCTGCTCAGGCTGCTGAGGCTGGCATGGCGTATTT
AGTATCCAAGGTCAAAGGTTTGGGGGGCGTGATCGTTATTGATGCCGAAGGCAGATGTGGTGCTGGCCACTCCACCTCCG
GCATGATTTATGGTTGGATTGAGCATGGGGGTGATTCCCATTTTCAATTGACCTAG

Upstream 100 bases:

>100_bases
CAACAGATTCTGTCTATGGATCCCAAGTGAGGATTATCCTTGTGGCGATCTTGTATATGGAATCAAGTGATACCCTTTTA
GTCTCCCGTTCTCCCCCATC

Downstream 100 bases:

>100_bases
CAGCCTGTTCCATGCGGTCTGCTGGAAGTCGTTTGGGCCGTTAAATAAATACGGCCTTGCGCATCCTGAGTCCATCCTTT
TGCTTCAACAATCTGGATGG

Product: L-asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSSTYSLMIHGGAGALQHIQAEGSEEDFAQSINGILEQGRQKLQQGELALNVVEHCVSLLEDDPLYNAGRGSVLNEYGEV
EMDAAIMNGQDLAAGAITGIKNIKNPISLARLVLKKSEHVMLVGHGAMEFAELWGVECLPDEYFVIEARVKQFQEAKKAG
KMVLDHEDIEQEPQRKFGTVGATAFDLEGNLAAATSTGGIVNKRWGRVGDSPIIGAGVFADNETCAVSATGYGEQFMRTV
LAKTISDHILFQSLTAAQAAEAGMAYLVSKVKGLGGVIVIDAEGRCGAGHSTSGMIYGWIEHGGDSHFQLT

Sequences:

>Translated_311_residues
MSSTYSLMIHGGAGALQHIQAEGSEEDFAQSINGILEQGRQKLQQGELALNVVEHCVSLLEDDPLYNAGRGSVLNEYGEV
EMDAAIMNGQDLAAGAITGIKNIKNPISLARLVLKKSEHVMLVGHGAMEFAELWGVECLPDEYFVIEARVKQFQEAKKAG
KMVLDHEDIEQEPQRKFGTVGATAFDLEGNLAAATSTGGIVNKRWGRVGDSPIIGAGVFADNETCAVSATGYGEQFMRTV
LAKTISDHILFQSLTAAQAAEAGMAYLVSKVKGLGGVIVIDAEGRCGAGHSTSGMIYGWIEHGGDSHFQLT
>Mature_310_residues
SSTYSLMIHGGAGALQHIQAEGSEEDFAQSINGILEQGRQKLQQGELALNVVEHCVSLLEDDPLYNAGRGSVLNEYGEVE
MDAAIMNGQDLAAGAITGIKNIKNPISLARLVLKKSEHVMLVGHGAMEFAELWGVECLPDEYFVIEARVKQFQEAKKAGK
MVLDHEDIEQEPQRKFGTVGATAFDLEGNLAAATSTGGIVNKRWGRVGDSPIIGAGVFADNETCAVSATGYGEQFMRTVL
AKTISDHILFQSLTAAQAAEAGMAYLVSKVKGLGGVIVIDAEGRCGAGHSTSGMIYGWIEHGGDSHFQLT

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=305, Percent_Identity=43.6065573770492, Blast_Score=218, Evalue=8e-57,
Organism=Homo sapiens, GI145275200, Length=305, Percent_Identity=43.6065573770492, Blast_Score=218, Evalue=8e-57,
Organism=Homo sapiens, GI98991769, Length=322, Percent_Identity=32.9192546583851, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI285002251, Length=227, Percent_Identity=34.3612334801762, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI285002253, Length=227, Percent_Identity=31.7180616740088, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1787050, Length=301, Percent_Identity=43.5215946843854, Blast_Score=239, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI32564649, Length=221, Percent_Identity=28.0542986425339, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI18921183, Length=309, Percent_Identity=36.8932038834951, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24652135, Length=281, Percent_Identity=33.8078291814947, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI221330102, Length=281, Percent_Identity=33.8078291814947, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI21357893, Length=273, Percent_Identity=32.6007326007326, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24657358, Length=239, Percent_Identity=30.5439330543933, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33113; Mature: 32982

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTYSLMIHGGAGALQHIQAEGSEEDFAQSINGILEQGRQKLQQGELALNVVEHCVSLL
CCCEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDDPLYNAGRGSVLNEYGEVEMDAAIMNGQDLAAGAITGIKNIKNPISLARLVLKKSEHV
CCCCCCCCCCCCHHHHCCCEEEEHEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCE
MLVGHGAMEFAELWGVECLPDEYFVIEARVKQFQEAKKAGKMVLDHEDIEQEPQRKFGTV
EEEECCHHHHHHHCCCEECCCCEEEEHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHCCC
GATAFDLEGNLAAATSTGGIVNKRWGRVGDSPIIGAGVFADNETCAVSATGYGEQFMRTV
CCEEEECCCCEEEECCCCCCCHHHCCCCCCCCEEEEEEEECCCEEEEEECCCHHHHHHHH
LAKTISDHILFQSLTAAQAAEAGMAYLVSKVKGLGGVIVIDAEGRCGAGHSTSGMIYGWI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEE
EHGGDSHFQLT
ECCCCCCEEEC
>Mature Secondary Structure 
SSTYSLMIHGGAGALQHIQAEGSEEDFAQSINGILEQGRQKLQQGELALNVVEHCVSLL
CCEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDDPLYNAGRGSVLNEYGEVEMDAAIMNGQDLAAGAITGIKNIKNPISLARLVLKKSEHV
CCCCCCCCCCCCHHHHCCCEEEEHEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCE
MLVGHGAMEFAELWGVECLPDEYFVIEARVKQFQEAKKAGKMVLDHEDIEQEPQRKFGTV
EEEECCHHHHHHHCCCEECCCCEEEEHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHCCC
GATAFDLEGNLAAATSTGGIVNKRWGRVGDSPIIGAGVFADNETCAVSATGYGEQFMRTV
CCEEEECCCCEEEECCCCCCCHHHCCCCCCCCEEEEEEEECCCEEEEEECCCHHHHHHHH
LAKTISDHILFQSLTAAQAAEAGMAYLVSKVKGLGGVIVIDAEGRCGAGHSTSGMIYGWI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEE
EHGGDSHFQLT
ECCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]