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The map label for this gene is glcD [H]

Identifier: 158334251

GI number: 158334251

Start: 1037472

End: 1038941

Strand: Direct

Name: glcD [H]

Synonym: AM1_1070

Alternate gene names: 158334251

Gene position: 1037472-1038941 (Clockwise)

Preceding gene: 158334250

Following gene: 158334252

Centisome position: 15.95

GC content: 50.0

Gene sequence:

>1470_bases
ATGATTGCCACAGATACTCAACGCAATTGGCAACCTATCATCCGACAAATGCAGACTATTGTCGGCCAAGACGGAGTAAT
GGTGACCCCAGAGGAGCTATCCGTCTATGAATGCGATGGTTTAGCCAGTTATCGGCAACGTCCTAGCTTAGTTGTGCTGC
CCCAAACAACGGCAGAAGTGGCAGCAGCGGTAGAGATTTGCGATCGCAACCAGATACCCTTTGTAGCTCGAGGCTCTGGA
ACGGGTTTGTCTGGCGGAGCAATGCCCACGGAAGGTTGTGTGTTAATTGTCACCTCACGCATGAATAAAATTCTTGAAAT
TGATATTGAGAACCAATGCGTGGTGGTCCAACCCGGGGTGATTAATGCTTGGGTGACCCAGGCGGTCGCACCTAATGGCT
TTTACTATGCCCCCGATCCGTCTAGCCAGATTATTTGCTCCATCGGTGGCAATATTGCCGAAAACTCTGGTGGTGTTCAT
TGTCTCAAGTATGGGGTAACCACCAACCATGTCTTGGGAATGAAAATGGTCCTCACCAATGGTGAGGTGGTGGATCTAGG
GGGCAAGGTTGCAGATATGCCAGGTTATGACCTGACAGGGGTGTTTGTGGGGTCGGAAGGAACCTTAGGCATTGCCACGG
AAATTACCCTGAGAATTTTACGCAGCGCTGATGCTATTTCCGTACTACTTGCCGATTTCACCACCATTGATGCCGCCGGG
AAAACCGTTGCCGACATTATCGGGGCAGGTGTGATTCCCGCTGGTATGGAAATTATGGATAACTTCAGCATTAACGCGGT
GGAGGATGTGGTGGCAACAGACTGCTATCCGCGCGATGCGGGTTCTATCCTCTTGATTGAGCTGGATGGTCTGCAAGTTG
AAGTGAATGCCAGTAGTGAACGGGTTAAAGAAATTTGCCGCCAAAATGGTGCTCGAAATATCACCGTAGCCACAGAGCCA
GAAGAACGGCTGAGGCTGTGGCAGGGACGGAAAGCAGCGTTTGCGGCTGCTGGTAAGCTTAGTCCCAACTATTTTGTTCA
AGATGGGGTAATTCCTAGAACAGAACTGGCCTATGTCTTAGGCGAAATTAATACTCTCGGTGAAAAGTTTGGCTACCGCA
TTGCCAATGTTTTCCATGCCGGAGATGGCAATTTACATCCCTTGATTCTTTATGATGAGTCGGTTCCGGGGTCTTTGGAA
AAGGTGGAAGAGTTAGGCGGCGAAATTCTGAAGCTCTGTGTGCGCGTTGGCGGCAGTATTTCCGGTGAACATGGCATTGG
AGCGGATAAGCGCTGCTATATGCCGGATATGTTTTCGGAGGCGGATTTAGAAACGATGCAATGGGTTCGCCATGTATTTA
ACCCCAAGGAGCTAGCCAATCCTGGCAAGGTGTTTCCTACACCTCGCACCTGTGGGGAAGGGGCCCATACCCATTTACCT
GTGGAGATTGATGGGGCAATGCTGTTTTAA

Upstream 100 bases:

>100_bases
GCCTCCTAGATTGAGCTTCCTCAGCCTGAGTGGGGCATGATTCTGGTCTAAGCTAGGCATAGGTCATTGCGGCCTGCCCC
TTACTGCAGACTATATCCTC

Downstream 100 bases:

>100_bases
GCAAGGTGTTGCATAAAATCACTCAGATTCGGTTTCTTTTCGCTAATGTTCAATTTCATCGGAAGCTTGTGCTGCCGTGC
TCTGCTGCAGTACCAATTCT

Product: glycolate oxidase GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MIATDTQRNWQPIIRQMQTIVGQDGVMVTPEELSVYECDGLASYRQRPSLVVLPQTTAEVAAAVEICDRNQIPFVARGSG
TGLSGGAMPTEGCVLIVTSRMNKILEIDIENQCVVVQPGVINAWVTQAVAPNGFYYAPDPSSQIICSIGGNIAENSGGVH
CLKYGVTTNHVLGMKMVLTNGEVVDLGGKVADMPGYDLTGVFVGSEGTLGIATEITLRILRSADAISVLLADFTTIDAAG
KTVADIIGAGVIPAGMEIMDNFSINAVEDVVATDCYPRDAGSILLIELDGLQVEVNASSERVKEICRQNGARNITVATEP
EERLRLWQGRKAAFAAAGKLSPNYFVQDGVIPRTELAYVLGEINTLGEKFGYRIANVFHAGDGNLHPLILYDESVPGSLE
KVEELGGEILKLCVRVGGSISGEHGIGADKRCYMPDMFSEADLETMQWVRHVFNPKELANPGKVFPTPRTCGEGAHTHLP
VEIDGAMLF

Sequences:

>Translated_489_residues
MIATDTQRNWQPIIRQMQTIVGQDGVMVTPEELSVYECDGLASYRQRPSLVVLPQTTAEVAAAVEICDRNQIPFVARGSG
TGLSGGAMPTEGCVLIVTSRMNKILEIDIENQCVVVQPGVINAWVTQAVAPNGFYYAPDPSSQIICSIGGNIAENSGGVH
CLKYGVTTNHVLGMKMVLTNGEVVDLGGKVADMPGYDLTGVFVGSEGTLGIATEITLRILRSADAISVLLADFTTIDAAG
KTVADIIGAGVIPAGMEIMDNFSINAVEDVVATDCYPRDAGSILLIELDGLQVEVNASSERVKEICRQNGARNITVATEP
EERLRLWQGRKAAFAAAGKLSPNYFVQDGVIPRTELAYVLGEINTLGEKFGYRIANVFHAGDGNLHPLILYDESVPGSLE
KVEELGGEILKLCVRVGGSISGEHGIGADKRCYMPDMFSEADLETMQWVRHVFNPKELANPGKVFPTPRTCGEGAHTHLP
VEIDGAMLF
>Mature_489_residues
MIATDTQRNWQPIIRQMQTIVGQDGVMVTPEELSVYECDGLASYRQRPSLVVLPQTTAEVAAAVEICDRNQIPFVARGSG
TGLSGGAMPTEGCVLIVTSRMNKILEIDIENQCVVVQPGVINAWVTQAVAPNGFYYAPDPSSQIICSIGGNIAENSGGVH
CLKYGVTTNHVLGMKMVLTNGEVVDLGGKVADMPGYDLTGVFVGSEGTLGIATEITLRILRSADAISVLLADFTTIDAAG
KTVADIIGAGVIPAGMEIMDNFSINAVEDVVATDCYPRDAGSILLIELDGLQVEVNASSERVKEICRQNGARNITVATEP
EERLRLWQGRKAAFAAAGKLSPNYFVQDGVIPRTELAYVLGEINTLGEKFGYRIANVFHAGDGNLHPLILYDESVPGSLE
KVEELGGEILKLCVRVGGSISGEHGIGADKRCYMPDMFSEADLETMQWVRHVFNPKELANPGKVFPTPRTCGEGAHTHLP
VEIDGAMLF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:
GO:0003824: Glycolate oxidase, subunit GlcD
GO:0008762: Glycolate oxidase, subunit GlcD
GO:0008891: Glycolate oxidase, subunit GlcD
GO:0009339: Glycolate oxidase, subunit GlcD
GO:0016491: Glycolate oxidase, subunit GlcD
GO:0016614: Glycolate oxidase, subunit GlcD
GO:0050660: Glycolate oxidase, subunit GlcD
GO:0055114: Glycolate oxidase, subunit GlcD

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=438, Percent_Identity=30.8219178082192, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI37595754, Length=461, Percent_Identity=29.5010845986985, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI119964728, Length=451, Percent_Identity=27.2727272727273, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI4501993, Length=224, Percent_Identity=25.4464285714286, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789351, Length=460, Percent_Identity=50.8695652173913, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1787977, Length=498, Percent_Identity=27.1084337349398, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI48994907, Length=498, Percent_Identity=22.6907630522088, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71992373, Length=457, Percent_Identity=27.5711159737418, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI71992381, Length=457, Percent_Identity=25.601750547046, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI71992392, Length=358, Percent_Identity=27.9329608938547, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17534361, Length=476, Percent_Identity=25.6302521008403, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17556096, Length=219, Percent_Identity=23.7442922374429, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320027, Length=436, Percent_Identity=28.6697247706422, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6320764, Length=468, Percent_Identity=23.5042735042735, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320023, Length=487, Percent_Identity=24.0246406570842, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI18921117, Length=460, Percent_Identity=26.9565217391304, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24639277, Length=460, Percent_Identity=26.9565217391304, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24639275, Length=460, Percent_Identity=26.9565217391304, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24653753, Length=228, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 52368; Mature: 52368

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATDTQRNWQPIIRQMQTIVGQDGVMVTPEELSVYECDGLASYRQRPSLVVLPQTTAEV
CCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEECCCHHHHHCCCCEEEECCCHHHH
AAAVEICDRNQIPFVARGSGTGLSGGAMPTEGCVLIVTSRMNKILEIDIENQCVVVQPGV
HHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCEEEECCCC
INAWVTQAVAPNGFYYAPDPSSQIICSIGGNIAENSGGVHCLKYGVTTNHVLGMKMVLTN
HHHHHHHHCCCCCCEECCCCCCCEEEEECCCEECCCCCEEEEEECCCCCCEEEEEEEEEC
GEVVDLGGKVADMPGYDLTGVFVGSEGTLGIATEITLRILRSADAISVLLADFTTIDAAG
CCEEECCCEEECCCCCCEEEEEECCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
KTVADIIGAGVIPAGMEIMDNFSINAVEDVVATDCYPRDAGSILLIELDGLQVEVNASSE
HHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCCCCCCEEEEEECCEEEEECCCHH
RVKEICRQNGARNITVATEPEERLRLWQGRKAAFAAAGKLSPNYFVQDGVIPRTELAYVL
HHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCHHHHHHHH
GEINTLGEKFGYRIANVFHAGDGNLHPLILYDESVPGSLEKVEELGGEILKLCVRVGGSI
HHHHHHHHHHCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
SGEHGIGADKRCYMPDMFSEADLETMQWVRHVFNPKELANPGKVFPTPRTCGEGAHTHLP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCEECC
VEIDGAMLF
EEECCEEEC
>Mature Secondary Structure
MIATDTQRNWQPIIRQMQTIVGQDGVMVTPEELSVYECDGLASYRQRPSLVVLPQTTAEV
CCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEECCCHHHHHCCCCEEEECCCHHHH
AAAVEICDRNQIPFVARGSGTGLSGGAMPTEGCVLIVTSRMNKILEIDIENQCVVVQPGV
HHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCEEEECCCC
INAWVTQAVAPNGFYYAPDPSSQIICSIGGNIAENSGGVHCLKYGVTTNHVLGMKMVLTN
HHHHHHHHCCCCCCEECCCCCCCEEEEECCCEECCCCCEEEEEECCCCCCEEEEEEEEEC
GEVVDLGGKVADMPGYDLTGVFVGSEGTLGIATEITLRILRSADAISVLLADFTTIDAAG
CCEEECCCEEECCCCCCEEEEEECCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
KTVADIIGAGVIPAGMEIMDNFSINAVEDVVATDCYPRDAGSILLIELDGLQVEVNASSE
HHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCCCCCCEEEEEECCEEEEECCCHH
RVKEICRQNGARNITVATEPEERLRLWQGRKAAFAAAGKLSPNYFVQDGVIPRTELAYVL
HHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCHHHHHHHH
GEINTLGEKFGYRIANVFHAGDGNLHPLILYDESVPGSLEKVEELGGEILKLCVRVGGSI
HHHHHHHHHHCCEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
SGEHGIGADKRCYMPDMFSEADLETMQWVRHVFNPKELANPGKVFPTPRTCGEGAHTHLP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCEECC
VEIDGAMLF
EEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]