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The map label for this gene is proA [C]

Identifier: 158334190

GI number: 158334190

Start: 976277

End: 977527

Strand: Reverse

Name: proA [C]

Synonym: AM1_1006

Alternate gene names: 158334190

Gene position: 977527-976277 (Counterclockwise)

Preceding gene: 158334191

Following gene: 158334188

Centisome position: 15.03

GC content: 47.64

Gene sequence:

>1251_bases
ATGGATGCTTCTCAAAGATTTGACCTGAACACAACAGTGCATCAGGCTCACATTGCCTCGCAGAAACTAGCACTCCTGCC
CCATCCAAAACGTAATGAATTGCTGCGTTGTTTGGCTCAAATCTTGCAAGATCGCCAAGATGATTTGTTAGAAGCAAACA
CCATAGATTTAGAAGTCAGCCGTGATCTAGCGATTCCTTCCTTAGTTCTGAACTGGCTAAAGCTCGCTCCAGAACGTCTG
CAAACCTTGGGGCTAATGTTGAACCATTTGGCGACAACAACTGACCCGTTGGCGATGATAGCCTTTCCCCAGTCCACTTT
TATGGTGGCTCCAGGCTATGCCAAGTTACAACCCCGGGGAGTTATTGCCTTTATTTATGAGGCGTTTCCAGACTTAGCGC
TGTTACTTGCCGGCATGTGCTGGAAAACGGGTAATGCGTTACTGCTGAAGGGAGGAGCTGAAACGAAGCATTCTAATCAG
GTTGTTATCGAGTTGCTTCATGCTGCCCTGGCCGCCGCTGATTTGCCCTTAACTTGTGTGCAATGCATCCCTTGCGATCG
CAACATTTCAATCTCCGATCTGGTTGCCCGAGACCTACCTATCGATCTTGTTGTTCCCTATGGTCGCCCCAGCTTGGTTC
AACAGGTTGTGCAGCAATCCACCGTCCCCACGCTTCAGTCTGCGATGGGCAATTGCTATCTTTTTTGGTCTGACTCGGGC
AGTACAGACCTAACCCGATCCATGATTATCAATAGTCATCAAGGTAGTCCAGATGCGGTCAATGCCATTGAAAAAGTCTT
GCTAACCCCCAACATTAAGCAGTCTCGACTCACGCTCCTATGGAGTACCCTCAAGGAAAAAGGATTCGAATTACGAGGAG
ATGCCACCCTAGCAGCTGAGTTTCCAGAGTTAATTCTGGCAACCAAGGAAGAGTGGCAGCAGCCTTATCTACAAAAAATT
GTGGCTTTTCGCGTCGTGGATGATTTAGCCGATGCCCTTAACTGGATTAACCGCCACAGTAGTCATCATGCCGATTGTTT
AGTCACGGATTCTTATCAAGACAGTCAACAATTTATCCAAGGTGTTCGTAGTTCTCAAGTGTTTATCAACACCAGCCCCC
AATTTTTGCGACAGTCCAGCGGGCCTGGAGGGTCTCTGGCGATAGGCATGAGTTCAGCCAAAAGCCTTTCTCCTGGGGTG
ATTAGCCTTGAAACCTTAATGACTTCCAAACAAATTATTCAAACCTCATAA

Upstream 100 bases:

>100_bases
CTAAGGCGGGAGTTTCGCCTCGGCCTTGCTGTATTCATGGCAGTCCCCACTCTCCAACTCGGTTCATATCTTTATCGACG
TTAATGGATGTATTTTGCCT

Downstream 100 bases:

>100_bases
CGGCTCCTTGAGCAAGGATCTGTTAACCAAAGGTTGAACCATTCCATCCCCACATCATGCCCTTTGCGTCCGCAAACTCA
ATATAGATTCGCTGCTTTGG

Product: gamma-glutamyl phosphate reductase

Products: L-glutamyl 5-phosphate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MDASQRFDLNTTVHQAHIASQKLALLPHPKRNELLRCLAQILQDRQDDLLEANTIDLEVSRDLAIPSLVLNWLKLAPERL
QTLGLMLNHLATTTDPLAMIAFPQSTFMVAPGYAKLQPRGVIAFIYEAFPDLALLLAGMCWKTGNALLLKGGAETKHSNQ
VVIELLHAALAAADLPLTCVQCIPCDRNISISDLVARDLPIDLVVPYGRPSLVQQVVQQSTVPTLQSAMGNCYLFWSDSG
STDLTRSMIINSHQGSPDAVNAIEKVLLTPNIKQSRLTLLWSTLKEKGFELRGDATLAAEFPELILATKEEWQQPYLQKI
VAFRVVDDLADALNWINRHSSHHADCLVTDSYQDSQQFIQGVRSSQVFINTSPQFLRQSSGPGGSLAIGMSSAKSLSPGV
ISLETLMTSKQIIQTS

Sequences:

>Translated_416_residues
MDASQRFDLNTTVHQAHIASQKLALLPHPKRNELLRCLAQILQDRQDDLLEANTIDLEVSRDLAIPSLVLNWLKLAPERL
QTLGLMLNHLATTTDPLAMIAFPQSTFMVAPGYAKLQPRGVIAFIYEAFPDLALLLAGMCWKTGNALLLKGGAETKHSNQ
VVIELLHAALAAADLPLTCVQCIPCDRNISISDLVARDLPIDLVVPYGRPSLVQQVVQQSTVPTLQSAMGNCYLFWSDSG
STDLTRSMIINSHQGSPDAVNAIEKVLLTPNIKQSRLTLLWSTLKEKGFELRGDATLAAEFPELILATKEEWQQPYLQKI
VAFRVVDDLADALNWINRHSSHHADCLVTDSYQDSQQFIQGVRSSQVFINTSPQFLRQSSGPGGSLAIGMSSAKSLSPGV
ISLETLMTSKQIIQTS
>Mature_416_residues
MDASQRFDLNTTVHQAHIASQKLALLPHPKRNELLRCLAQILQDRQDDLLEANTIDLEVSRDLAIPSLVLNWLKLAPERL
QTLGLMLNHLATTTDPLAMIAFPQSTFMVAPGYAKLQPRGVIAFIYEAFPDLALLLAGMCWKTGNALLLKGGAETKHSNQ
VVIELLHAALAAADLPLTCVQCIPCDRNISISDLVARDLPIDLVVPYGRPSLVQQVVQQSTVPTLQSAMGNCYLFWSDSG
STDLTRSMIINSHQGSPDAVNAIEKVLLTPNIKQSRLTLLWSTLKEKGFELRGDATLAAEFPELILATKEEWQQPYLQKI
VAFRVVDDLADALNWINRHSSHHADCLVTDSYQDSQQFIQGVRSSQVFINTSPQFLRQSSGPGGSLAIGMSSAKSLSPGV
ISLETLMTSKQIIQTS

Specific function: Catalyzes The Nadph Dependent Reduction Of L-Gamma- Glutamyl 5-Phosphate Into L-Glutamate 5-Semialdehyde And Phosphate. The Product Spontaneously Undergoes Cyclization To Form 1-Pyrroline-5-Carboxylate. [C]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=408, Percent_Identity=27.2058823529412, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI62912457, Length=408, Percent_Identity=27.2058823529412, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1786438, Length=408, Percent_Identity=29.9019607843137, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17569967, Length=401, Percent_Identity=26.6832917705736, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17569969, Length=401, Percent_Identity=26.6832917705736, Blast_Score=134, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6324899, Length=438, Percent_Identity=24.4292237442922, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21357643, Length=407, Percent_Identity=27.5184275184275, Blast_Score=128, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: 1.2.1.41

Molecular weight: Translated: 45682; Mature: 45682

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDASQRFDLNTTVHQAHIASQKLALLPHPKRNELLRCLAQILQDRQDDLLEANTIDLEVS
CCCCCCCCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
RDLAIPSLVLNWLKLAPERLQTLGLMLNHLATTTDPLAMIAFPQSTFMVAPGYAKLQPRG
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCCCC
VIAFIYEAFPDLALLLAGMCWKTGNALLLKGGAETKHSNQVVIELLHAALAAADLPLTCV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
QCIPCDRNISISDLVARDLPIDLVVPYGRPSLVQQVVQQSTVPTLQSAMGNCYLFWSDSG
HEECCCCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHCCCEEEEECCCC
STDLTRSMIINSHQGSPDAVNAIEKVLLTPNIKQSRLTLLWSTLKEKGFELRGDATLAAE
CCHHHHHHHEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEECCCEEEHHH
FPELILATKEEWQQPYLQKIVAFRVVDDLADALNWINRHSSHHADCLVTDSYQDSQQFIQ
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHH
GVRSSQVFINTSPQFLRQSSGPGGSLAIGMSSAKSLSPGVISLETLMTSKQIIQTS
HHCCCEEEEECCHHHHHHCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MDASQRFDLNTTVHQAHIASQKLALLPHPKRNELLRCLAQILQDRQDDLLEANTIDLEVS
CCCCCCCCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
RDLAIPSLVLNWLKLAPERLQTLGLMLNHLATTTDPLAMIAFPQSTFMVAPGYAKLQPRG
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCCCC
VIAFIYEAFPDLALLLAGMCWKTGNALLLKGGAETKHSNQVVIELLHAALAAADLPLTCV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
QCIPCDRNISISDLVARDLPIDLVVPYGRPSLVQQVVQQSTVPTLQSAMGNCYLFWSDSG
HEECCCCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHCCCEEEEECCCC
STDLTRSMIINSHQGSPDAVNAIEKVLLTPNIKQSRLTLLWSTLKEKGFELRGDATLAAE
CCHHHHHHHEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEECCCEEEHHH
FPELILATKEEWQQPYLQKIVAFRVVDDLADALNWINRHSSHHADCLVTDSYQDSQQFIQ
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHH
GVRSSQVFINTSPQFLRQSSGPGGSLAIGMSSAKSLSPGVISLETLMTSKQIIQTS
HHCCCEEEEECCHHHHHHCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 2.5 {DL-pyrroline-5-carboxylic} 11 {phosphate}} 0.18 {NADP+}} 0.05 {NADP+}} 2.2 {glutamic} [C]

Substrates: L-glutamate 5-semialdehyde; phosphate; NADP+

Specific reaction: L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: 3-(Phosphonoacetyl amido)-L-alanine; Ba2+; Co2+; Cu2+; Iodoacetamide; Iodoacetate; NADPH; Ni2+; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]