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The map label for this gene is livM [H]

Identifier: 158334048

GI number: 158334048

Start: 839764

End: 840942

Strand: Reverse

Name: livM [H]

Synonym: AM1_0862

Alternate gene names: 158334048

Gene position: 840942-839764 (Counterclockwise)

Preceding gene: 158334049

Following gene: 158334047

Centisome position: 12.93

GC content: 49.62

Gene sequence:

>1179_bases
ATGATTACAGATTCTTCTCCCGTCAAGTCCTCATTTGCCATGCGCCGACGCTCTTTATTGATCGAAGGGGTGATCGTGGT
TGCGATCGCGCTCTTTATCCAATTTGGTGCGCCCCTACTACTCACCGCCATCGGCCAGGAATTCCGCATCAATATGCTGG
GCCGCTTTATGTCCTTAGCGATTGTGGCTTTGGGTATTGATCTGATCTGGGGCTACACTGGCATGCTTAGCCTCGGCCAC
GGTATCTTTTTCTCCTTAGGAGGATATGCCCTAGGCATGTTTCTACAGCTCCAACTCCCGGAAGGTCAGCTCCCAGAGTT
TTTTACCTTATTTGGGGTAGACAAGCTCCCCGGGTTTTGGCAACCCTTTCACTCCCTACCCTTTACCCTGGCAGCCATTG
TGTTGATTCCGGCTTCAATTGCTGCCCTACTCGGCTACTTAGTGTTTCGCAATCGCATTCGAGGAGTGTACTTCTCGATC
CTGACCCAAGCGTCTCTGATTGTGTTCTTTAACTTTTTCAATAGCCAACAGAAGCTGATTAACGGCACGAATGGCCTTAA
AACTGATACTACGAAAATATTTGGAGTCTATGCAGGATCAGACCAAGCCCAGTCTGTTTTCTACACCCTCACCGTTATTT
TCTTAGTCTTGGCCTACTTACTCTGTCGATGGTTGACCAGTGGTCGGCTGGGCCGATTATTGATTGCGATTCGGGATGAC
GAAAGTCGGGTGAGATTTTCAGGTTACGATCCAACGGGTTTCAAGGTTCTGGTCTTTGCCGTATCAGCAGGACTTGCCGG
AATTGCTGGCGCACTCTATACCGTCCAATCAGGGATTATCTCTCCCAAGACGATGGATATCGCCTTTTCCATTGAGATGG
TCATTTGGGTTGCCGTTGGGGGTAGAGCCAGTTTAGTCGGTGCCATTTTTGGGGCATTGGCCGTTAATTTTGCCAAAAGT
CTTTTGAGTGAGCAGTTCCCCGAAATTTGGCTGTTTTTCCAGGGGGCTTTGTTCTTGGTGGTTGTTACCGTCCTCCCCGG
TGGATTTGTCGGTTGGCTGCGCAACGAAGGACAGGAACTGCTCCGCTCTGTACTGGGTCGACCGCGGCGAATCATGACCT
ACCCTAGTTTGGCTGAAGATCCGGAAGTAGACTATGAGCGACAAAATCTTAGAAATTGA

Upstream 100 bases:

>100_bases
GAAAGTGCTGGTCTTTGCACTGATTGTCACGTTCTTGCAATTCCGACCTGCTGGCATATTCCCTCAAAAAGGACGCATGG
TAGATGCTTAAAAGCAGCAT

Downstream 100 bases:

>100_bases
AAATTTAACCGTCAGTTTTGAAGGGTTTAAAGCCCTGAACCAGCTCAACTTCAGCATGGACACCGGAGAGCTGCGGGTCA
TTATTGGTCCCAATGGCGCT

Product: urea/ branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MITDSSPVKSSFAMRRRSLLIEGVIVVAIALFIQFGAPLLLTAIGQEFRINMLGRFMSLAIVALGIDLIWGYTGMLSLGH
GIFFSLGGYALGMFLQLQLPEGQLPEFFTLFGVDKLPGFWQPFHSLPFTLAAIVLIPASIAALLGYLVFRNRIRGVYFSI
LTQASLIVFFNFFNSQQKLINGTNGLKTDTTKIFGVYAGSDQAQSVFYTLTVIFLVLAYLLCRWLTSGRLGRLLIAIRDD
ESRVRFSGYDPTGFKVLVFAVSAGLAGIAGALYTVQSGIISPKTMDIAFSIEMVIWVAVGGRASLVGAIFGALAVNFAKS
LLSEQFPEIWLFFQGALFLVVVTVLPGGFVGWLRNEGQELLRSVLGRPRRIMTYPSLAEDPEVDYERQNLRN

Sequences:

>Translated_392_residues
MITDSSPVKSSFAMRRRSLLIEGVIVVAIALFIQFGAPLLLTAIGQEFRINMLGRFMSLAIVALGIDLIWGYTGMLSLGH
GIFFSLGGYALGMFLQLQLPEGQLPEFFTLFGVDKLPGFWQPFHSLPFTLAAIVLIPASIAALLGYLVFRNRIRGVYFSI
LTQASLIVFFNFFNSQQKLINGTNGLKTDTTKIFGVYAGSDQAQSVFYTLTVIFLVLAYLLCRWLTSGRLGRLLIAIRDD
ESRVRFSGYDPTGFKVLVFAVSAGLAGIAGALYTVQSGIISPKTMDIAFSIEMVIWVAVGGRASLVGAIFGALAVNFAKS
LLSEQFPEIWLFFQGALFLVVVTVLPGGFVGWLRNEGQELLRSVLGRPRRIMTYPSLAEDPEVDYERQNLRN
>Mature_392_residues
MITDSSPVKSSFAMRRRSLLIEGVIVVAIALFIQFGAPLLLTAIGQEFRINMLGRFMSLAIVALGIDLIWGYTGMLSLGH
GIFFSLGGYALGMFLQLQLPEGQLPEFFTLFGVDKLPGFWQPFHSLPFTLAAIVLIPASIAALLGYLVFRNRIRGVYFSI
LTQASLIVFFNFFNSQQKLINGTNGLKTDTTKIFGVYAGSDQAQSVFYTLTVIFLVLAYLLCRWLTSGRLGRLLIAIRDD
ESRVRFSGYDPTGFKVLVFAVSAGLAGIAGALYTVQSGIISPKTMDIAFSIEMVIWVAVGGRASLVGAIFGALAVNFAKS
LLSEQFPEIWLFFQGALFLVVVTVLPGGFVGWLRNEGQELLRSVLGRPRRIMTYPSLAEDPEVDYERQNLRN

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=318, Percent_Identity=25.4716981132075, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 43135; Mature: 43135

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDSSPVKSSFAMRRRSLLIEGVIVVAIALFIQFGAPLLLTAIGQEFRINMLGRFMSLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHH
IVALGIDLIWGYTGMLSLGHGIFFSLGGYALGMFLQLQLPEGQLPEFFTLFGVDKLPGFW
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHCCCCCCCHH
QPFHSLPFTLAAIVLIPASIAALLGYLVFRNRIRGVYFSILTQASLIVFFNFFNSQQKLI
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
NGTNGLKTDTTKIFGVYAGSDQAQSVFYTLTVIFLVLAYLLCRWLTSGRLGRLLIAIRDD
CCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
ESRVRFSGYDPTGFKVLVFAVSAGLAGIAGALYTVQSGIISPKTMDIAFSIEMVIWVAVG
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHEEEEEEEEEEEEC
GRASLVGAIFGALAVNFAKSLLSEQFPEIWLFFQGALFLVVVTVLPGGFVGWLRNEGQEL
CHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRSVLGRPRRIMTYPSLAEDPEVDYERQNLRN
HHHHHCCCCEEEECCCCCCCCCCCCHHHCCCC
>Mature Secondary Structure
MITDSSPVKSSFAMRRRSLLIEGVIVVAIALFIQFGAPLLLTAIGQEFRINMLGRFMSLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHH
IVALGIDLIWGYTGMLSLGHGIFFSLGGYALGMFLQLQLPEGQLPEFFTLFGVDKLPGFW
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHCCCCCCCHH
QPFHSLPFTLAAIVLIPASIAALLGYLVFRNRIRGVYFSILTQASLIVFFNFFNSQQKLI
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
NGTNGLKTDTTKIFGVYAGSDQAQSVFYTLTVIFLVLAYLLCRWLTSGRLGRLLIAIRDD
CCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
ESRVRFSGYDPTGFKVLVFAVSAGLAGIAGALYTVQSGIISPKTMDIAFSIEMVIWVAVG
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHEEEEEEEEEEEEC
GRASLVGAIFGALAVNFAKSLLSEQFPEIWLFFQGALFLVVVTVLPGGFVGWLRNEGQEL
CHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRSVLGRPRRIMTYPSLAEDPEVDYERQNLRN
HHHHHCCCCEEEECCCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]