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The map label for this gene is mraW

Identifier: 158334035

GI number: 158334035

Start: 829527

End: 830423

Strand: Reverse

Name: mraW

Synonym: AM1_0849

Alternate gene names: 158334035

Gene position: 830423-829527 (Counterclockwise)

Preceding gene: 158334037

Following gene: 158334034

Centisome position: 12.77

GC content: 50.28

Gene sequence:

>897_bases
ATGACACCAGACCCTGGGGCCTTTAACCACATTTCCGTCTTAAGCCATGAACTGATGGCAGGACTGATGCTGCAGGAGCA
GGGCTTATATTTGGATGCCACCGTAGGCGGAGGCGGCCATAGTGCCTTGATCTTGTCCCAATATCCCCAGACTCAGGTGA
TCGCCCTTGATCAAGATATTCAGGCTTTAGAGGCCGCTCGAGCTAAGTTGCATGATGCTTGCGATCGCATCCAGTTTTGG
CAAGGAAATTTCTCCACCTATCACCCCGGTGAACTTCGGTTTGACGGCATTATCGCGGATTTAGGAGTGAGTTCAGCCCA
ACTGGATCGACCTGAGCGGGGTTTTAGTTTCCGCTACGACGCAGATCTAGATATGCGCATGGATCAGAGTCAAGGGTTGA
CCGCTGCCGATGTCATCAACACCTATTCAGAACGGGATTTAGCCGACATCTTCTATCACTATGGTGAAGAACGGTTTTCT
CGACGCATTGCCCGCAAAATTGTCTCCAAACGTCCCCTGCGTACAACCTCTGATTTAGCCCGGGTGGTGGCGTCTTCCCT
GCCTGCTGCCAAAGGGCGTCGCCGCCGAATTCATCCAGCAACCCGAGTCTTTCAAGCCCTGCGAATTGCCGTTAATCAGG
AATTGCAGGTCCTTGAAAAATTTATTGAGATTGCGCCTACCTGGCTCAAACCAGGGGGCAGAATTGGCATCATTAGTTTT
CATAGTCTGGAAGATCGAATTGTTAAAATTCACTTCCGTCAGAATTCTCTACTGCAGGTGGTCACCAAAAAACCGATTGT
TGCCTCTGAACAAGAGAAAAGAGAAAACAGCCGCTCTCGCTCCGCTAAGCTACGGTTTGCTGAACGGGTTCCTTTGGCAG
ATGAAGCTGAATCGTAA

Upstream 100 bases:

>100_bases
CAGCTTTGTGTTGAGTTTATTTCCTGGGCCAGGAGCCAGGTACAATCTGGAAGTTTAGGTTAGATCTAAGAACAGTACCT
TGTTCAACTCAAGGGAGTAT

Downstream 100 bases:

>100_bases
CGGATATAATGACGCCTGGTTTTGCAACTATCCTGTTTAGTGTTTGCAAAGGATAAACGAAGCGCTCTATTTATAGGGAA
GCAGCAACTTGATGCAAACT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTPDPGAFNHISVLSHELMAGLMLQEQGLYLDATVGGGGHSALILSQYPQTQVIALDQDIQALEAARAKLHDACDRIQFW
QGNFSTYHPGELRFDGIIADLGVSSAQLDRPERGFSFRYDADLDMRMDQSQGLTAADVINTYSERDLADIFYHYGEERFS
RRIARKIVSKRPLRTTSDLARVVASSLPAAKGRRRRIHPATRVFQALRIAVNQELQVLEKFIEIAPTWLKPGGRIGIISF
HSLEDRIVKIHFRQNSLLQVVTKKPIVASEQEKRENSRSRSAKLRFAERVPLADEAES

Sequences:

>Translated_298_residues
MTPDPGAFNHISVLSHELMAGLMLQEQGLYLDATVGGGGHSALILSQYPQTQVIALDQDIQALEAARAKLHDACDRIQFW
QGNFSTYHPGELRFDGIIADLGVSSAQLDRPERGFSFRYDADLDMRMDQSQGLTAADVINTYSERDLADIFYHYGEERFS
RRIARKIVSKRPLRTTSDLARVVASSLPAAKGRRRRIHPATRVFQALRIAVNQELQVLEKFIEIAPTWLKPGGRIGIISF
HSLEDRIVKIHFRQNSLLQVVTKKPIVASEQEKRENSRSRSAKLRFAERVPLADEAES
>Mature_297_residues
TPDPGAFNHISVLSHELMAGLMLQEQGLYLDATVGGGGHSALILSQYPQTQVIALDQDIQALEAARAKLHDACDRIQFWQ
GNFSTYHPGELRFDGIIADLGVSSAQLDRPERGFSFRYDADLDMRMDQSQGLTAADVINTYSERDLADIFYHYGEERFSR
RIARKIVSKRPLRTTSDLARVVASSLPAAKGRRRRIHPATRVFQALRIAVNQELQVLEKFIEIAPTWLKPGGRIGIISFH
SLEDRIVKIHFRQNSLLQVVTKKPIVASEQEKRENSRSRSAKLRFAERVPLADEAES

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=335, Percent_Identity=37.3134328358209, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI165377202, Length=190, Percent_Identity=40, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1786270, Length=315, Percent_Identity=43.1746031746032, Blast_Score=212, Evalue=2e-56,
Organism=Drosophila melanogaster, GI62472493, Length=326, Percent_Identity=35.8895705521472, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI28571637, Length=326, Percent_Identity=35.8895705521472, Blast_Score=157, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_ACAM1 (B0BYK8)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001515207.1
- ProteinModelPortal:   B0BYK8
- SMR:   B0BYK8
- GeneID:   5679676
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_0849
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- BioCyc:   AMAR329726:AM1_0849-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 33565; Mature: 33433

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: BINDING 57-57 BINDING 84-84 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDPGAFNHISVLSHELMAGLMLQEQGLYLDATVGGGGHSALILSQYPQTQVIALDQDI
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEEECHHH
QALEAARAKLHDACDRIQFWQGNFSTYHPGELRFDGIIADLGVSSAQLDRPERGFSFRYD
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEECCEEECCCCCHHHCCCCCCCCCEEEC
ADLDMRMDQSQGLTAADVINTYSERDLADIFYHYGEERFSRRIARKIVSKRPLRTTSDLA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RVVASSLPAAKGRRRRIHPATRVFQALRIAVNQELQVLEKFIEIAPTWLKPGGRIGIISF
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEEEE
HSLEDRIVKIHFRQNSLLQVVTKKPIVASEQEKRENSRSRSAKLRFAERVPLADEAES
CCCCCEEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TPDPGAFNHISVLSHELMAGLMLQEQGLYLDATVGGGGHSALILSQYPQTQVIALDQDI
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEEECHHH
QALEAARAKLHDACDRIQFWQGNFSTYHPGELRFDGIIADLGVSSAQLDRPERGFSFRYD
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEECCEEECCCCCHHHCCCCCCCCCEEEC
ADLDMRMDQSQGLTAADVINTYSERDLADIFYHYGEERFSRRIARKIVSKRPLRTTSDLA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RVVASSLPAAKGRRRRIHPATRVFQALRIAVNQELQVLEKFIEIAPTWLKPGGRIGIISF
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEEEE
HSLEDRIVKIHFRQNSLLQVVTKKPIVASEQEKRENSRSRSAKLRFAERVPLADEAES
CCCCCEEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA