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The map label for this gene is bar [H]

Identifier: 158333963

GI number: 158333963

Start: 754724

End: 755251

Strand: Reverse

Name: bar [H]

Synonym: AM1_0777

Alternate gene names: 158333963

Gene position: 755251-754724 (Counterclockwise)

Preceding gene: 158333976

Following gene: 158333962

Centisome position: 11.61

GC content: 47.92

Gene sequence:

>528_bases
ATGTCAGACTCAGAGCCGGTTCAGATTCGAGCAAGTACATCAGCGGATTATGCTGCGATCGCATCTATATACAATGAAGC
GATCGCAACAGGCAGTATCACCTTCGATACTCGTCAATTCGAAGCGTCAGATATTCAAGCCTGGGTGAATAAATTTTGCG
ATCGCGAATGTCTGTTGGTCATTGAGCGTCAACAGCAGGTTCTAGGCTGGGGCATCGTCAAACAATATAGCGATCGCCCT
GGGTATCGGATCTGCTGCGAAACATCGATTTATCTGACCTTTGCAGAAAAGGGCCAAGGGTTAGGGAAAATGATGCAAAC
GGCTCTACTGCAAAAGGTGGTTGGGCTAGGATATCACCACGTAGTCGTAAAAATTGTCGCGACCAATGATGACAGCATTG
CCTTCCACAAAAGCTTTGGGTTTGAAATGGTCGGTGTGCAAAGAGAAGTTGGGCATGTTGCCGATCGCTGGCAAGATGTT
GCCATCATGCAGCTTATTCTGCCCCAAGTTCCACCCTACCCATCATGA

Upstream 100 bases:

>100_bases
CCCTGCCCTAGGAGAGATATTTTCCTATCCATGAGGGAGGTATAACTTGTCTACCTAGACGTGCTAGAACTGGCTTGAGT
TGCCAGGGAAAACGCCTTAC

Downstream 100 bases:

>100_bases
AAAGTAGGAGTTCTTGAGTTTTCTCTCTGCTGCCTAGATGAGAAAAATGTTCGGATGGATTCTGATGGGAATTGGAGTGC
TACTGATTTTCTTTGGCAGT

Product: acetyltransferase

Products: NA

Alternate protein names: PPT N-acetyltransferase; Phosphinothricin-resistance protein [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MSDSEPVQIRASTSADYAAIASIYNEAIATGSITFDTRQFEASDIQAWVNKFCDRECLLVIERQQQVLGWGIVKQYSDRP
GYRICCETSIYLTFAEKGQGLGKMMQTALLQKVVGLGYHHVVVKIVATNDDSIAFHKSFGFEMVGVQREVGHVADRWQDV
AIMQLILPQVPPYPS

Sequences:

>Translated_175_residues
MSDSEPVQIRASTSADYAAIASIYNEAIATGSITFDTRQFEASDIQAWVNKFCDRECLLVIERQQQVLGWGIVKQYSDRP
GYRICCETSIYLTFAEKGQGLGKMMQTALLQKVVGLGYHHVVVKIVATNDDSIAFHKSFGFEMVGVQREVGHVADRWQDV
AIMQLILPQVPPYPS
>Mature_174_residues
SDSEPVQIRASTSADYAAIASIYNEAIATGSITFDTRQFEASDIQAWVNKFCDRECLLVIERQQQVLGWGIVKQYSDRPG
YRICCETSIYLTFAEKGQGLGKMMQTALLQKVVGLGYHHVVVKIVATNDDSIAFHKSFGFEMVGVQREVGHVADRWQDVA
IMQLILPQVPPYPS

Specific function: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group [H]

COG id: COG1247

COG function: function code M; Sortase and related acyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787719, Length=164, Percent_Identity=29.8780487804878, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.183 [H]

Molecular weight: Translated: 19551; Mature: 19419

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSEPVQIRASTSADYAAIASIYNEAIATGSITFDTRQFEASDIQAWVNKFCDRECLLV
CCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHEEH
IERQQQVLGWGIVKQYSDRPGYRICCETSIYLTFAEKGQGLGKMMQTALLQKVVGLGYHH
HHHHHHHHHHHHHHHHCCCCCEEEEEECEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHH
VVVKIVATNDDSIAFHKSFGFEMVGVQREVGHVADRWQDVAIMQLILPQVPPYPS
EEEEEEEECCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDSEPVQIRASTSADYAAIASIYNEAIATGSITFDTRQFEASDIQAWVNKFCDRECLLV
CCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHEEH
IERQQQVLGWGIVKQYSDRPGYRICCETSIYLTFAEKGQGLGKMMQTALLQKVVGLGYHH
HHHHHHHHHHHHHHHHCCCCCEEEEEECEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHH
VVVKIVATNDDSIAFHKSFGFEMVGVQREVGHVADRWQDVAIMQLILPQVPPYPS
EEEEEEEECCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916276; 12000953; 2160942 [H]